Genetic engineering techniques recent developments:proceedings of the symposium on recent advances in genetic engineering held January 11-12, 1982, Taiwan, Republic of china/ edited by P.C. Haung, T.t. Kuo and Ray Wu

Genetic Engineering Techniques: Recent Developments covers the proceedings of the 1982 Genetic Engineering Techniques symposium held in Taipei. The book is organized into 21 chapters that discuss the application of recombination DNA methods in the study of DNA structure and DNA-protein interactions; the use of chemically synthesized genes in cloning; and gene expression. After briefly presenting the major strategies underlying genetic engineering technology and rapid method for sequencing DNA, the book examines the reaction mechanism of a multifunctional Type I enzyme and the organization and expression of the human adenovirus. The second section describes several approaches in analyzing transcriptional processes in prokaryotic and eukaryotic systems. This section also deals with cloning vectors and procedures of cDNA. The subsequent section describes a molecular approach to functional analysis of the influenza virus surface hemagglutinin; the transposition specificity for the transposons 3 and 4 elements; and the biological properties of human T-cell growth factor gene. The fourth section discusses the principles of hybridoma technology and its numerous applications to biological research. The remaining chapters of the book present laboratory courses designed to familiarize researchers with the principles and basic procedures in biological experiments. Genetic engineering researchers, agriculturists, and geneticists will find this book invaluable. Content: Inside Front Cover , Page ii Front Matter , Page iii Copyright , Page iv CONTRIBUTORS , Pages ix-xi PREFACE , Pages xiii-xiv , P.C. Huang, T.T. Kuo, Ray Wu GENETIC ENGINEERING TECHNOLOGY - AN OVERVIEW AND SOME RECENT ADVANCES , Pages 3-21 , Ray Wu, Li-He Guo, Richard C. Scarpulla THE METHYLATED STATE OF A DNA SEQUENCE AND ITS EFFECT ON THE FUNCTIONS OF THE RESTRICTION ENDONUCLEASE FROM E. COLI K. , Pages 23-39 , Robert Yuan, Jean Burckhardt, Daniel L. Hamilton THE APPLICATION OF RECOMBINANT DNA METHODS TO STRUCTURAL STUDIES OF DNA , Pages 41-56 , James C. Wang ORGANIZATION AND EXPRESSION OF THE HUMAN ADENOVIRUS GENES ENCODING THE DNA BINDING PROTEIN AND THE DNA TERMINAL PROTEIN , Pages 57-90 , Louise T. Chow, Mara Rossini, Jeffrey A. Engler, Mark P. van Bree, Richard A. Guilfoyle, Thomas R. Broker SOME APPROACHES FOR ANALYZING TRANSCRIPTIONAL PROCESSES IN PROKARYOTIC AND EUKARYOTIC SYSTEMS , Pages 93-110 , Ru-Chih C. Huang CLONING OF DOUBLE STRANDED cDNA FROM MAJOR AND MINOR COMPONENTS OF mRNA , Pages 111-127 , Yuan Lin NUCLEOTIDE SEQUENCE, DNA MODIFICATION, AND IN VITRO TRANSCRIPTION OF ALU FAMILY REPEATS IN THE HUMAN α-LIKE GLOBIN GENE CLUSTER , Pages 129-158 , Che-Kun James Shen, Tom Maniatis STUDY ON THE PRIMARY STRUCTURE OF THE ISOLEUCYL-tRNA SYNTHETASE FROM ESCHERICHIA COLI MRE 600 , Pages 159-172 , Hsin Tsai, Maria-Regina Kula, Werner Lindenmaier EXPRESSION OF THE CLONED p21 TRANSFORMING ras GENE OF HARVEY MURINE SARCOMA VIRUS , Pages 175-188 , Thomas Y. Shih INFLUENZA A VIRUSES AS STUDIED BY RECOMBINANT DNA TECHNIQUES , Pages 189-202 , Ching-Juh Lai, Lewis J. Markoff, Michael M. Sveda, Bor-Chian Lin, Robert A. Lamb SPECIFICITY OF Tn3 AND Tn4 TRANSPOSITION , Pages 203-224 , Chen-Pei D. Tu, Kurt P. Timmerman, David R. Hyde HUMAN T-CELL GROWTH FACTOR (TCGF) BIOLOGICAL PROPERTIES AND ISOLATION OF BIOLOGICALLY ACTIVE mRNA , Pages 225-239 , Robert C. Ting, Mark M. Manak EXPRESSION OF EUKARYOTIC GENES IN BACILLUS SUBTILIS , Pages 243-250 , Shing Chang, Sheng-Yung Chang, Diana Ho, Oanh Gray, James Kroyer, Jane McLaughlin EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN MAMMALIAN CELLS , Pages 251-262 , Chung-Cheng Liu, Craig Crowley, Arthur D. Levinson MONOCLONAL ANTIBODIES: PREPARATION BY HYBRIDOMA METHOD AND APPLICATIONS IN BIOLOGICAL RESEARCH , Pages 263-281 , Tse-Wen Chang New Vistas of Genetics-1982 , Pages 285-295 , P.C. Huang PRODUCTION OF MONOCLONAL ANTIBODIES BY HYBRIDOMA METHOD , Pages 299-324 , Tse-Wen Chang, Nancy T. Chang Electron Microscopy , Pages 325-327 , Thomas R. Broker, Louise Chow Isolation of Recombinant Clones from Genomic DNA Library by In Situ Hybridization , Pages 329-331 , James Shen PLASMID TRANSFORMATION AND PREPARATION , Pages 333-336 , Shing Chang THE PURIFICATION OF RESTRICTION ENZYMES AND DNA SUBSTRATES. THEIR USE IN DNA SEQUENCING , Pages 337-356 , Robert Yuan, Yuan Lin INDEX , Pages 357-360