Functional Genomics and Proteomics in the Clinical Neurosciences (Volume 158) (Progress in Brain Research, Volume 158)

The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses. * Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry * Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies * Use of actual data to illustrate many principles of functional genomics and proteomics. * Introduction to bioinformatics and database management techniques Content: List of Contributors Pages v-vi Foreword Pages vii-viii Stephen H. Koslow Tissue preparation and banking Review Article Pages 3-14 Maree J. Webster Functional genomic methodologies Review Article Pages 15-40 Stephen D. Ginsberg, Károly Mirnics Methods for proteomics in neuroscience Review Article Pages 41-82 Nilesh S. Tannu, Scott E. Hemby Functional genomics and proteomics in the clinical neurosciences: data mining and bioinformatics Review Article Pages 83-108 John H. Phan, Chang-Feng Quo, May D. Wang Reproducibility of microarray studies: concordance of current analysis methods Review Article Pages 109-125 Matt T. Wayland, Sabine Bahn The genomics of mood disorders Review Article Pages 129-139 Salvatore Alesci, Michelle Rodak, Ioannis Ilias, Rulun Zhou, Husseini K. Manji Transcriptome alterations in schizophrenia: disturbing the functional architecture of the dorsolateral prefrontal cortex Review Article Pages 141-152 David A. Lewis, Karoly Mirnics Strategies for improving sensitivity of gene expression profiling: regulation of apoptosis in the limbic lobe of schizophrenics and bipolars Review Article Pages 153-172 Francine M. Benes Assessment of genome and proteome profiles in cocaine abuse Review Article Pages 173-195 Scott E. Hemby Neuronal gene expression profiling: uncovering the molecular biology of neurodegenerative disease Review Article Pages 197-222 Elliott J. Mufson, Scott E. Counts, Shaoli Che, Stephen D. Ginsberg Epileptogenesis-related genes revisited Review Article Pages 223-241 Katarzyna Lukasiuk, Michal Dabrowski, Alicja Adach, Asla Pitkänen Functional genomics of sex hormone-dependent neuroendocrine systems: specific and generalized actions in the CNS Review Article Pages 243-272 Anna W. Lee, Nino Devidze, Donald W. Pfaff, Jin Zhou Implications for the practice of psychiatry Review Article Pages 275-293 Elisabeth B. Binder, Charles B. Nemeroff Human brain evolution Review Article Pages 295-309 Hilliary Creely, Philipp Khaitovich Subject Index Pages 311-317 Erratum to Progress in Brain Research Vol. 158 Functional Genomics and Proteomics in the Clinical Neurosciences Scott E. Hemby and Sabine Bahn Page 318 The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses.

* Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry
* Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies
* Use of actual data to illustrate many principles of functional genomics and proteomics.
* Introduction to bioinformatics and database management techniques