Farm Animal Proteomics 2013: Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 and 3 of COST Action FA1002 Kosice, Slovakia - 25-26 April 2013

Proteomics may be defined as the large-scale study of the proteome, i.e. a set of proteins being expressed in a certain fluid, tissue, organ or organism. Although still of limited and restricted use in most areas of farm animal and veterinary research, proteomics potential is unequivocal holding a significant promise in applications such as vaccine and drug development, animal product quality, physiology or toxicology. Nevertheless, proteomics use has been growing steadily during the last 2-3 years and, as time goes by; proteomics-based studies are more and more common, not just to scientists but to the general public, unravelling their full potential. This book reflects the will of a group of scientists that merge innovation with excellence of research and to whom the dissemination of knowledge and innovation through cooperation is a key essential point. It will be of interest to scientists at the early stages of their careers as well as to researchers well established in the field and to whom proteomics may be the necessary next step towards more in-depth research activities. By providing a collection of diverse scientific interests, Farm Animal Proteomics 2013 is also a witness to the vitality of the area and the importance it holds to animal and food research, to science, industry, government agencies, the consumer and ultimately the society as a whole. Front Matter....Pages 1-15 Front Matter....Pages 17-17 Mining deeper into the proteome: pros and cons of pre-fractionation and depletion....Pages 19-20 Detection and annotation of common post-translational modifications in mass spectrometry data....Pages 21-22 Applied bioinformatics in the structural, post-genomic era....Pages 23-25 PTMomics – a potpourri of experimental approaches....Pages 26-26 Gel-free quantitative proteomics approaches, current status....Pages 27-27 New proteomics strategies applied to clinical studies....Pages 28-29 Neuro-immune proteomic crosstalk in health and disease: partners in love, partners in divorce....Pages 30-30 Top-down proteomics: 2D gels are an integral part of the process....Pages 31-33 MALDI imaging mass spectrometry: applications, limitations and potential....Pages 34-35 Parasite- and host-derived proteins involved in African trypanosome brain invasion and dysfunction....Pages 36-36 Proteomics at the host: pathogen interface....Pages 37-37 Microbial proteomics in food safety and animal welfare....Pages 38-39 The search of stress markers in porcine by using proteomics....Pages 40-42 Quest for biomarkers of the lean-to-fat ratio by proteomics in beef production....Pages 43-44 The LEXSY platform for recombinant protein expression....Pages 45-48 Front Matter....Pages 49-49 Data-independent acquisition strategies for quantitative proteomics....Pages 51-54 Small intestinal response to enterotoxigenic Escherichia coli infection in pigs as revealed by label free UPLC/MS E proteomics....Pages 55-58 Rapid protein production of Atlantic Salmon, Salmo salar , serum amyloid A (SAA) in an inducible Leishmania tarentolae expression system....Pages 59-62 Proteomic profiling of cerebrospinal fluid in canine degenerative myelopathy....Pages 63-66 Automatic prediction of PTMs in Ehrlichia ruminantium – creating new datasets for Quickmod analyses....Pages 67-70 Front Matter....Pages 49-49 Tandem mass spectrometry for species recognition and phenotyping in fish....Pages 71-74 MALDI imaging mass spectrometry of ageing and osteoarthritic cartilage....Pages 75-78 Protein expression in bovine mononuclear cells after stimulation with lipopolysaccharides and lipoteichoic acid: a proteomic approach....Pages 79-83 The ‘hidden’ proteome of cow’s and Jennys’ milk as revealed by combinatorial peptide ligand libraries....Pages 84-88 Comparison between Coomassie Blue and Silver staining in porcine saliva samples for proteomics: technical considerations: a preliminary experiment....Pages 89-92 Isoelectric focusing in characterisation of alkaline phosphatase isozyme from bovine nasal secretion and mucosa....Pages 93-96 Front Matter....Pages 97-97 PilE4 may contributes in the adhesion of Francisella to brain microvascular endothelial cells....Pages 99-102 Comparative proteomics analysis of campylobacteriosis in human and swine: a study of intestine epithelial cell response to bacterial infection....Pages 103-106 Identification of amino acid residues of OspA of Borrelia involved in binding to CD40 receptor....Pages 107-111 Omics approaches to study the Rickettsia Ehrlichia ruminantium : towards improved knowledge on Heartwater disease....Pages 112-115 Detection of Salmonella antigens expressed in swine gut by hydrophobic antigen tissue triton extraction (HATTREX)....Pages 116-119 New approaches in the diagnosis and treatment of endotoxemia in calves: proteomic investigation and effects of intravenous choline administration....Pages 120-123 Changes on bovine aorta endothelial cells (BAE) proteome upon infection with the rickettsia Ehrlichia ruminantium ....Pages 124-127 Effect of Staphylococcus aureus infection on fibrinogen and plasma protein concentrations in experimentally obese rabbits....Pages 128-131 Trypanosoma brucei brucei binds human complement regulatory protein C4BP....Pages 132-134 A proteomic analysis of canine serum during the course of babesiosis....Pages 135-138 The acute phase reaction in goats after experimentally induced E. coli mastitis: a proteomic approach....Pages 139-142 The effect of microbial challenge on the intestinal proteome of broiler chickens....Pages 143-146 2D DIGE comparative analysis of Escherichia coli strains with induced resistance to enrofloxacin....Pages 147-150 In situ assessment of differential adhesion of neuroinvasive and non-neuroinvasive Borrelia strains to BMECs....Pages 151-154 Front Matter....Pages 97-97 Caecal IEL, blood lymphocytes and intestinal mucin study in chickens after probiotic prevention and S. Enteritidis infection....Pages 155-158 Bovine anaplasmosis: IgG2 reactivity in vaccinated cattle against conserved recombinant outer membrane proteins....Pages 159-162 Identification BMECs receptors interacting with Trypanosoma brucei brucei ....Pages 163-166 Effect of short-time overweight gain on the plasma PON1 activity after acute Staphylococcus aureus infection in rabbits....Pages 167-171 Front Matter....Pages 173-173 Biomarkers of winter disease in gilthead seabream: a proteomics approach....Pages 175-178 The absorption of colostral proteins in newborn lambs: an iTRAQ proteomics study....Pages 179-183 Plasma proteome profiles predict diet-induced metabolic syndrome and the early onset of metabolic syndrome in a pig model....Pages 184-187 Changes in protein abundance in the vertebral columnof Atlantic salmon ( Salmosalar ) fed variable dietary P levels....Pages 188-191 Neurotransmitter levels and proteomic approach in pig brain: pre-slaughter handling stress and cognitive biases....Pages 192-195 The sheep ( Ovis aries ) mammary gland mitochondrial complexes: establishment of a Blue-Native PAGE separation method as a model for other ruminant species....Pages 196-198 Bitter taste in water-buffalo ( Bubalus bubalis ): from T2R gene identification to expression studies....Pages 199-203 Seasonal weight loss in dairy goats from the Canary Islands: towards an integrated omics approach?....Pages 204-208 Analysis of endogenous and recombinant bovine somatotropin in serum....Pages 209-212 Investigation of salivary acute phase proteins in calves....Pages 213-216 Targeted proteomics as a tool for porcine acute phase proteins measurements....Pages 217-220 LC-MS/MS analyses of visceral and subcutaneous adipose tissue proteomes in goats....Pages 221-225 Comparison of the patterns of milk serum proteins in subclinical mastitis caused by four common pathogens in dairy cows....Pages 226-229 Intrauterine treated lambs as a model for the study of Intrauterine Growth Restriction (IUGR) and consequent metabolic disorders....Pages 230-233 Effect of estradiol on biochemical bone metabolism markers in dairy cows....Pages 234-237 Dynamics of metabolic and oxidative stress parameters in dairy heifers during transition period....Pages 238-241 Front Matter....Pages 173-173 The proteomics of wool follicles....Pages 242-244 Relationships between mediators of the acute phase response and altered energetic metabolism in dairy cows after calving....Pages 245-248 Serum protein electrophoretic pattern in clinically healthy calves and cows....Pages 249-252 Front Matter....Pages 253-253 A mass spectrometric scoring system for oxidative damage in dairy foods....Pages 255-257 Shotgun proteomics in blue mussels exposed to benthic trawler-induced sediment resuspension from a polluted fjord....Pages 258-261 Milk and cheese microbiome for safety and quality of dairy products....Pages 262-265 Molecular characterization of Maltese honey: diastase and proline levels changes in Maltese honey seasons....Pages 266-269 Untargeted metabolomic analyses open new scenarios in post mortem pig muscles: Casertana and Large White....Pages 270-273 The role of salt in dry cured ham processing characterized by LC-MS/MS-based proteomics....Pages 274-277 Proteolytic action of caspases 3 and 7 on the hydrolysis of bovine and porcine muscle myofibrillar proteins....Pages 278-281 Allergen characterization in Mediterranean farmed fish: a proteomic approach....Pages 282-286 Protein exudation from salted meat in processing by cold tumbling....Pages 287-289 Peptidomics in extended shelf life dairy products, for assessment of proteolysis and product quality....Pages 290-294 Egg proteome: the protein and carotenoids characteristics deriving from laying hens reared in different housing systems and under different environmental conditions....Pages 295-297 Proteomics and transcriptomics investigation on longissimus muscles in Large White and Casertana pig breeds....Pages 298-301 Back Matter....Pages 303-304 Proteomics May Be Defined As The Large-scale Study Of The Proteome, I.e. A Set Of Proteins Being Expressed In A Certain Fluid, Tissue, Organ Or Organism. Although Still Of Limited And Restricted Use In Most Areas Of Farm Animal And Veterinary Research, Proteomics Potential Is Unequivocal Holding A Significant Promise In Applications Such As Vaccine And Drug Development, Animal Product Quality, Physiology Or Toxicology. Nevertheless, Proteomics Use Has Been Growing Steadily During The Last 2-3 Years And, As Time Goes By; Proteomics-based Studies Are More And More Common, Not Just To Scientists But To The General Public, Unraveling Their Full Potential. This Book Reflects The Will Of A Group Of Scientists That Merge Innovation With Excellence Of Research And To Whom The Dissemination Of Knowledge And Innovation Through Cooperation Is A Key Essential Point. It Will Be Of Interest To Scientists At The Early Stages Of Their Careers As Well As To Researchers Well Established In The Field And To Whom Proteomics May Be The Necessary Next Step Towards More In-depth Research Activities. By Providing A Collection Of Diverse Scientific Interests, Farm Animal Proteomics 2013 Is Also A Witness To The Vitality Of The Area And The Importance It Holds To Animal And Food Research, To Science, Industry, Government Agencies, The Consumer And Ultimately The Society As A Whole.--page 4 Of Cover. Edited By Andre De Almeida [and 6 Others]. Includes Bibliographical References. Mode Of Access: World Wide Web. "Proteomics may be defined as the large-scale study of the proteome, i.e. a set of proteins being expressed in a certain fluid, tissue, organ or organism. Although still of limited and restricted use in most areas of farm animal and veterinary research, proteomics potential is unequivocal holding a significant promise in applications such as vaccine and drug development, animal product quality, physiology or toxicology. Nevertheless, proteomics use has been growing steadily during the last 2-3 years and, as time goes by; proteomics-based studies are more and more common, not just to scientists but to the general public, unraveling their full potential. This book reflects the will of a group of scientists that merge innovation with excellence of research and to whom the dissemination of knowledge and innovation through cooperation is a key essential point. It will be of interest to scientists at the early stages of their careers as well as to researchers well established in the field and to whom proteomics may be the necessary next step towards more in-depth research activities. By providing a collection of diverse scientific interests, Farm Animal Proteomics 2013 is also a witness to the vitality of the area and the importance it holds to animal and food research, to science, industry, government agencies, the consumer and ultimately the society as a whole."-- Taken from publisher web site Small intestinal response to enterotoxigenic Escherichia coli infection in pigs as revealed by label free UPLC/MSE proteomicsObjectives; Material and methods; Results and discussion; Acknowledgements; References; Rapid protein production of Atlantic Salmon, Salmo salar, serum amyloid A (SAA) in an inducible Leishmania tarentolae expression system; Introduction; Materials and methods; Results and discussion; Conclusion; Acknowledgements; References; Proteomic profiling of cerebrospinal fluid in canine degenerative myelopathy; Objectives; Material and methods; Results and discussion; References Proteomics at the host: pathogen interfaceMicrobial proteomics in food safety and animal welfare; References; The search of stress markers in porcine by using proteomics; Acknowledgements; References; Quest for biomarkers of the lean-to-fat ratio by proteomics in beef production; References; The LEXSY platform for recombinant protein expression; References; Part II Advancing methodology for farm animal proteomics and bioinformatics; Data-independent acquisition strategies for quantitative proteomics; Objectives; Material and methods; Results and discussion; Acknowledgements; References The Cost Organisation; Editors; Scientific committee for the book of proceedings; Table of contents; Farm animal proteomics: going to the European capital of culture 2013; Part I Invited plenary communications; Mining deeper into the proteome: pros and cons of pre-fractionation and depletion; References; Detection and annotation of common posttranslational modifications in mass spectrometry data; Objectives; Material and methods; Results and discussion; References; Applied bioinformatics in the structural, postgenomic era; Objectives; Material and methods; Results and discussion AcknowledgementsReferences; PTMomics - a potpourri of experimental approaches; References; Gel-free quantitative proteomics approaches, current status; New proteomics strategies applied to clinical studies; Neuro-immune proteomic crosstalk in health and disease: partners in love, partners in divorce; Acknowledgements; Top-down proteomics: 2D gels are an integral part of the process; References; MALDI imaging mass spectrometry: applications, limitations and potential; References; Parasiteand host-derived proteins involved in African trypanosome brain invasion and dysfunction Automatic prediction of PTMs in Ehrlichia ruminantium - creating new datasets for Quickmod analysesObjectives; Material and methods; Results and discussion; Acknowledgements; References; Tandem mass spectrometry for species recognition and phenotyping in fish; Objectives; Material and methods; Results and discussion; Acknowledgment; References; MALDI imaging mass spectrometry of ageing and osteoarthritic cartilage; Aim; Introduction; Methods; Results; Discussion; References