Epitope Mapping Protocols : Second Edition

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In __Epitope Mapping Protocols, Second Edition__, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular __Methods in Molecular BiologyTM__ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, __Epitope Mapping Protocols, Second Edition__ is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping. What is a B-cell epitope? / Marc H.V. Van Regenmortel Structural basis of antibody-antigen interactions / Eric J. Sundberg Epitope mapping of antibody-antigen complexes by nuclear magnetic resonance spectroscopy / Osnat Rosen and Jacob Anglister A solid-phase mutual inhibition assay with labeled antigen / Masahide Kuroki Epitope mapping by surface plasmon resonance / Pär Säfsten Proteolytic fragmentation for epitope mapping / Maria R. Mazzoni, Francesca Porchia, and Heidi E. Hamm Epitope mapping by proteolysis of antigen-antibody complexes / Suraj Dhungana ... [et al.] Identifying residues in antigenic determinants by chemical modification / Howard M. Reisner and Roger L. Lundblad Epitope mapping by differential chemical modification of antigens / Suraj Dhungana, Michael B. Fessler, and Kenneth B. Tomer Linear B-cell epitope mapping using enzyme-linked immunosorbent assay for libraries of overlapping synthetic peptides / Michael W. Heuzenroeder ... [et al.] Antibody epitope mapping using SPOT peptide arrays / Ulrich Reineke and Robert Sabat Peptide microarrays for profiling of modification state-specific antibodies / Johannes Zerweck, Antonia Masch, and Mike Schutkowski Epitope mapping using phage display peptide libraries / Volker Böttger and Angelika Böttger Antibody epitope mapping using de novo generated synthetic peptide libraries / Ulrich Reineke Antibody specificity profiling on functional protein microarrays / Dawn R. Mattoon and Barry Schweitzer Peptide microarrays for determination of cross-reactivity / Alexandra Thiele Epitope mapping using randomly generated peptide libraries / Juliane Bongartz, Nicole Bruni, and Michal Or-Guil Probing the epitope signatures of IgG antibodies in human serum from patients with autoimmune disease / Peter Lorenz ... [et al.] Microarrayed allergen molecules for diagnostics of allergy / Jing Lin, Ludmilla Bardina, and Wayne G. Shreffler Monitoring B cell response to immunoselected phage-displayed peptides by microarrays / Lina Cekaite, Eiving Hovig, and Mouldy Sioud Epitope mapping using homolog-scanning mutagenesis / Lin-Fa Wang Epitope mapping by region-specified PCR-mutagenesis / Tsutomu Mikawa, Masayuki Ikeda, and Takehiko Shibata Epitope mapping using phage-display random fragment libraries / Lin-Fa Wang and Meng Yu Prediction of linear B-cell epitopes / Ulf Reimer Molecular recognition of diverse ligands by T-cell receptors / Eric J. Sundberg Identification of human MHC class I binding peptides using the iTOPIA epitope discovery system / Markus Wulf, Petra Hoehn, and Peter Trinder T-cell epitope mapping in Mycobacterium tuberculosis using PepMixes created by micro-scale SPOT synthesis / Marisa Frieder and David M. Lewinsohn High-throughput T-cell epitope discovery through MHC peptide exchange / Sine Reker Hadrup ... [et al.] T-cell epitope processing (the epitope flanking regions matter) / Alejandra Nacarino Martinez, Stefan Tenzer, and Hansjörg Schild Identification of MHC class II binding peptides : microarray and soluble MHC class II molecules / Simani Gaseitsiwe and Markus J. Maeurer T-cell epitope mapping / Raija K.S. Ahmed and Markus J. Maeurer Identification and validation of T-cell epitopes using the IFN-[gamma] EliSpot assay / Markus Wulf, Petra Hoehn, and Peter Trinder. "Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls." "Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping."--Jacket Front Matter....Pages i-xiii Front Matter....Pages 1-1 Front Matter....Pages 3-20 Front Matter....Pages 22-22 Front Matter....Pages 23-36 Front Matter....Pages 37-57 Front Matter....Pages 59-66 Front Matter....Pages 67-76 Back Matter....Pages 77-86 ....Pages 87-101