Enzymes and Proteins From Thermophilic Microorganisms Structure and Function : Proceedings of the International Symposium Zürich, July 28 to August 1, 1975

LIST OF PARTICIPANTS 11 THERMOPHILIC HYDROLYTIC ENZYMES (PROTEINASES, AMYLASES) 17 Thermal Stability of Homologous Neutral Metalloendopeptidases in Thermo­ philic and Mesophilic Bacteria: Structural Considerations M. K. PANGBURN, P. L. LEVY, K. A. WALSH and H. NEURATH 19 The Structure and Stability of Thermolysin L. H. WEAVER, W. R. KESTER, K. F. TEN EYCK and B. W. MATTHEWS 31 Studies of the Inhibition of Thermolysin J. FEDER, N. AUFDERHEIDE and B. S. WILDI 41 Effect of EDTA on the Conformational Stability of Thermolysin A. FONTANA, E. BOCCU and F. M. VERONESE 55 Role of a Sulfhydryl Group in the Structure and Function of Alkaline Proteases from a Thermophilic Actinomycete K. MIZUSAWA and F. YOSHIDA 61 Partial Characterization of a Thermophilic Actinomycete Rennin S. LAXER, A. PINSKY and B. BARTOOV 67 Amylase Activity and Stability at High and Low Temperature Depending on Calcium and other Divalent Cations W. HEINEN and A. M. LAUWERS 77 Role of Calcium Ion in the Thermostability of a-Amylase Produced from Bacillus stearothermophilus K. YUTAN I 91 THERMOPHILIC DEHYDROGENASES 105 Thermophilic Glyceraldehyde-3-P Dehydrogenase R. E. AMELUNXEN and R. SINGLETON JR. 107 Glyceraldehyde 3-Phosphate Dehydrogenase from an Extreme Thermophile, Thermus aquaticus 121 J. D. HOCKING and J. I. HARRIS Thermal Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Escherichia coli A. FONTANA, C. GRANDI, E. BOCCU and F. M. VERONESE 135 Comparative Conformational Properties of Thermophilic and Mesophilic 6-Phosphogluconate Dehydrogenase F. M. VERONESE, C. GRANDI, E. BOCCU and A. Front Matter....Pages 1-15 Front Matter....Pages 17-17 Thermal Stability of Homologous Neutral Metalloendopeptidases in Thermophilic and Mesophilic Bacteria: Structural Considerations....Pages 19-30 The Structure and Stability of Thermolysin....Pages 31-39 Studies on The Inhibition of Thermolysin....Pages 41-54 Effect of Edta on the Conformational Stability of Thermolysin....Pages 55-59 Role of a Sulfhydryl Group in the Structure and Function of Alkaline Proteases from a Thermophilic Actinomycete, Streptomyces rectus var. proteolyticus ....Pages 61-66 Partial Characterization of a Thermophilic Actinomycete Rennin....Pages 67-75 Amylase Activity and Stability at High and Low Temperature Depending on Calcium and Other Divalent Cations....Pages 77-89 Role of Calcium Ion in the Thermostability of α-Amylase Produced from Bacillus Stearothermophilus ....Pages 91-103 Front Matter....Pages 105-105 Thermophilic Glyceraldehyde-3-P Dehydrogenase....Pages 107-120 Glyceraldehyde 3-Phosphate Dehydrogenase from an Extreme Thermophile, Thermus Aquaticus ....Pages 121-133 Thermal Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Escherichia Coli....Pages 135-145 Comparative Conformational Properties of Thermophilic and Mesophilic 6-Phosphogluconate Dehydrogenase....Pages 147-155 Purification and Properties of Malate Dehydrogenase from Thermus Aquaticus....Pages 157-167 Purification and some Properties of NADP + -Specific Isocitrate Dehydrogenase from an Extreme Thermophile, Thermus flavus AT-62....Pages 169-183 Front Matter....Pages 185-185 Maintainance of Specificity, Information, and Thermostability in Thermophilic Bacillus sp. Glutamine Synthetase....Pages 187-197 Phosphofructokinase from Thermophilic Micro-Organisms....Pages 199-206 Purification and Catalytic Properties of “Thermostable” Fumarase from Bacillus Stearothermophilus NU-10 and Thermus X-1....Pages 207-222 Analysis of the Thermostability of Enolases....Pages 223-227 Isolation and some Properties of Enolase from Bacillus Stearothermophilus ....Pages 229-236 Properties of Enzymes from Clostridium Thermoaceticum and Clostridium Formicoaceticum....Pages 237-248 Front Matter....Pages 185-185 Chemical, Physical and Enzymatic Comparisons of Formyltetrahydrofolate Synthetases from Thermo- and Mesophilic Clostridia....Pages 249-262 Anaplerotic Enzymes of Acetate and Pyruvate Metabolism: Distinctive Characteristics in Bacillus Stearothermophilus ....Pages 263-275 Oxygen Induced Transformation of the NADH-Nitrate Oxidoreductase from Bacillus Stearothermophilus ....Pages 277-290 Thermophilic Phycocyanin....Pages 291-305 Ferredoxin from Bacillus Stearothermophilus ....Pages 307-313 Front Matter....Pages 315-315 Biochemical Studies on an Extreme Thermophile Thermus thermophilus : Thermal Stabilities of Cell Constituents and a Bacteriophage....Pages 317-331 The Effect of Polyamines on the Thermostability of a Cell Free Protein Synthesizing System of an Extreme Thermophile....Pages 333-345 Purification, Characterization and Mechanism of Action of Several Aminoacyl-tRNA Synthetases from Bacillus Stearothermophilus....Pages 347-362 Thermophilicity and Thermostability of RNA Polymerases from Two Strains of Caldariella Acidophila , an Extremely Thermo-Acidophilic Bacterium....Pages 363-373 Thermophilic and Mesophilic Enzymes from B. Caldotenax and B. Stearothermophilus: Properties, Relationships and Formation....Pages 375-389 Studies on the Genetical and Biochemical Basis of Thermophily....Pages 391-400 Bacterial Diversity at High Temperature....Pages 401-410 Round Table and General Discussion on Structure and Function of Thermophilic Enzymes and Proteins....Pages 411-438 Back Matter....Pages 439-445 LIST OF PARTICIPANTS 11 THERMOPHILIC HYDROLYTIC ENZYMES (PROTEINASES, AMYLASES) 17 Thermal Stability of Homologous Neutral Metalloendopeptidases in ThermoƯ philic and Mesophilic Bacteria: Structural Considerations M.K. PANGBURN, P.L. LEVY, K.A. WALSH and H. NEURATH 19 The Structure and Stability of Thermolysin L.H. WEAVER, W.R. KESTER, K.F. TEN EYCK and B.W. MATTHEWS 31 Studies of the Inhibition of Thermolysin J. FEDER, N. AUFDERHEIDE and B.S. WILDI 41 Effect of EDTA on the Conformational Stability of Thermolysin A. FONTANA, E. BOCCU and F.M. VERONESE 55 Role of a Sulfhydryl Group in the Structure and Function of Alkaline Proteases from a Thermophilic Actinomycete K. MIZUSAWA and F. YOSHIDA 61 Partial Characterization of a Thermophilic Actinomycete Rennin S. LAXER, A. PINSKY and B. BARTOOV 67 Amylase Activity and Stability at High and Low Temperature Depending on Calcium and other Divalent Cations W. HEINEN and A.M. LAUWERS 77 Role of Calcium Ion in the Thermostability of a-Amylase Produced from Bacillus stearothermophilus K. YUTAN I 91 THERMOPHILIC DEHYDROGENASES 105 Thermophilic Glyceraldehyde-3-P Dehydrogenase R.E. AMELUNXEN and R. SINGLETON JR. 107 Glyceraldehyde 3-Phosphate Dehydrogenase from an Extreme Thermophile, Thermus aquaticus 121 J.D. HOCKING and J.I. HARRIS Thermal Properties of Glyceraldehyde 3-Phosphate Dehydrogenase from Escherichia coli A. FONTANA, C. GRANDI, E. BOCCU and F.M. VERONESE 135 Comparative Conformational Properties of Thermophilic and Mesophilic 6-Phosphogluconate Dehydrogenase F.M. VERONESE, C. GRANDI, E. BOCCU and A