Dynamic studies in biology : phototriggers, photoswitches and caged biomolecules

With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods. With Contributions By More Than 30 Expert Researchers, This Handbook Covers The Whole Spectrum From Chemistry To Cell Biology And From Theory To Application. In So Doing, It Deals With A Broad Range Of Topics From The Chemistry And Biophysics Of Caged Compounds To Their Application In Time-resolved Studies, Comparing The Properties Of Different Caging Groups. The Authors Describe In Detail Light-activation Of Proteins As Well As Nucleic Acids, While A Special Section Is Devoted To Multiphoton Phototriggers. A Must-have For Every Biochemist, Biophysicist And Molecular Biologist Developing And W. Photoremovable Protecting Groups Used For The Caging Of Biomolecules -- Mechanistic Overview Of Phototriggers And Cage Release / Richard S. Givens, Mani B. Kotala, And Jong-ill Lee -- Caged Compounds And Solid-phase Synthesis / Yoshiro Tatsu, Yasushi Shigeri, And Noboru Yumoto -- Control Of Cellular Activity -- Photoregulation Of Proteins -- Photoremovable Protecting Groups In Dna Synthesis And Microarray Fabrication / Michael C. Pirrung And Vipul S. Rana -- Analytical Time-resolved Studies Using Photochemical Triggering Methods -- Multiphoton Phototriggers For Exploring Cell Physiology / Timothy M. Dore -- New Challenges. Edited By Maurice Goeldner, Richard Givens. Includes Bibliographical References And Index. The pioneering work of Kaplan et al. [7], although preceded by other examples of 2-nitrobenzyl photolysis in synthetic organic chemistry, was the first to apply this to a biological problem, the erythrocytic Na : K ion pump.