Dna Damage Detection In Situ, Ex Vivo, And In Vivo: Methods And Protocols (methods In Molecular Biology)

Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science. In Situ Detection Of Apoptosis By The Tunel Assay : An Overview Of Techniques / Deryk T. Loo -- Combination Of Tunel Assay With Immunohistochemistry For Simultaneous Detection Of Dna Fragmentation And Oxidative Cell Damage / Alexander E. Kalyuzhny -- Em-isel : A Useful Tool To Visualize Dna Damage At The Ultrastructural Level / Antonio Migheli -- In Situ Labeling Of Dna Breaks And Apoptosis By T7 Dna Polymerase / Vladimir V. Didenko -- In Situ Ligation : A Decade And A Half Of Experience / Peter J. Hornsby And Vladimir V. Didenko -- In Situ Ligation Simplified : Using Pcr Fragments For Detection Of Double-strand Dna Breaks In Tissue Sections / Vladimir V. Didenko -- 5'oh Dna Breaks In Apoptosis And Their Labeling By Topoisomerase-based Approach / Vladimir V. Didenko -- Detection Of Dna Strand Breaks In Apoptotic Cells By Flow- And Image-cytometry / Zbigniew Darzynkiewicz And Hong Zhao -- Fluorochrome-labeled Inhibitors Of Caspases : Convenient In Vitro And In Vivo Markers Of Apoptotic Cells For Cytometric Analysis / Zbigniew Darzynkiewicz ... [et Al.] -- Combining Fluorescent In Situ Hybridization With The Comet Assay For Targeted Examination Of Dna Damage And Repair / Sergey Shaposhnikov, Preben D. Thomsen, And Andrew R. Collins -- Simultaneous Labeling Of Single- And Double-strand Dna Breaks By Dna Breakage Detection-fish (dbd-fish) / José Luis Fernández, Dioleyda Cajigal, And Jaime Gosálvez -- Co-localization Of Dna Repair Proteins With Uv-induced Dna Damage In Locally Irradiated Cells / Jennifer Guerrero-santoro, Arthur S. Levine, And Vesna Rapić-otrin -- Ultrasound Imaging Of Apoptosis : Spectroscopic Detection Of Dna-damage Effects At High And Low Frequencies / Roxana M. Vlad, Michael C. Kolios, And Gregory J. Czarnota -- Quantifying Etheno-dna Adducts In Human Tissues, White Blood Cells, And Urine By Ultrasensitive 32p-postlabeling And Immunohistochemistry / Jagadeesan Nair ... [et Al.] -- Elispot Assay As A Tool To Study Oxidative Stress In Peripheral Blood Mononuclear Cells / Jodi Hagen, Jeffrey P. Houchins, And Alexander E. Kalyuzhny -- Cytokinesis-block Micronucleus Cytome Assay In Lymphocytes / Philip Thomas And Michael Fenech -- Buccal Micronucleus Cytome Assay / Philip Thomas And Michael Fenech -- [gamma]-h2ax Detection In Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts, And Skin / Christophe E. Redon ... [et Al.] -- Immunologic Detection Of Benzo(a)pyrene-dna Adducts / Regina M. Santella And Yu-jing Zhang -- Non-invasive Assessment Of Oxidatively Damaged Dna : Liquid Chromatography-tandem Mass Spectrometry Analysis Of Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine / Vilas Mistry ... [et Al.] -- Assessing Sperm Dna Fragmentation With The Sperm Chromatin Dispersion Test / José Luis Fernández ... [et Al.] Edited By Vladimir V. Didenko. Includes Bibliographical References And Index. Also Available Online. Front Matter....Pages i-xiii Front Matter....Pages 1-1 Front Matter....Pages 3-13 Front Matter....Pages 15-27 Front Matter....Pages 29-36 Back Matter....Pages 37-48 ....Pages 49-63