Diffusion chamber culture : hemopoiesis, cloning of tumors, cytogenetic and carcinogenic assays ; [proceedings of a Conference on Application of Diffusion Chamber Culture in Study of Hemopoiesis, Clonal Growth of Tumors, and Cytogenetic and Carcinogenic E

Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question. Front Matter....Pages I-XIII Front Matter....Pages 1-1 History and Overview of the In Vivo Diffusion Chamber (DC) Culture System....Pages 3-10 Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrow....Pages 11-18 Different Classes of Granulocyte Progenitor Cells Expressed in In Vivo Plasma Clot Diffusion Chamber Cultures....Pages 19-27 Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers....Pages 28-43 Studies on the Regulation of Diffusion Chamber Granulopoiesis....Pages 44-56 Megakaryocyte Growth in In Vitro Cultures and in Diffusion Chambers....Pages 57-61 Front Matter....Pages 63-63 Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinetics....Pages 65-71 Marrow Culture in Diffusion Chambers in Rabbits IV. Effects of Leukocyte Products on Granulopoiesis....Pages 72-79 Host-Mediated Regulatory Activities as Measured on Bone Marrow Cells Cultured in Diffusion Chambers and In Vitro....Pages 80-94 Growth of Canine Bone Marrow and Peripheral Blood Mononuclear Cells in In Vivo Plasma Clot Diffusion Chambers in Mice: Cytopoietic Activity, GM-CFC Content, and Physical Separation....Pages 95-109 Normal and Leukemic Hematopoiesis in Diffusion Chambers: Comparison with In Vitro Culture Systems....Pages 110-123 Stimulating Factor(s) of Hematopoiesis in the Bone Marrow of Mice Administered Hydroxyurea. Effects of Diffusion Chamber Progenitor Cells (DCPC) and Observations on Pluripotent Stem Cells (CFU-S)....Pages 124-135 Front Matter....Pages 137-137 Use of Diffusion Chambers for Studying Cell Interactions: Sequential Studies of Eosinophil Granulocytopoiesis In Vivo....Pages 139-149 An Evaluation of Host Cell Contact in the Regulation of Diffusion Chamber Hematopoiesis....Pages 150-156 Stem Cells in Human Peripheral Blood: A Method for Histologic Analysis of Colony Formation in Plasma Clot Diffusion Chambers....Pages 157-176 The Growth of Colonies of Human Bone Marrow and Tumour Cells in Soft Agar in Diffusion Chambers....Pages 177-187 Diffusion Chamber Cultures for Mutagenic and Carcinogenic Assays....Pages 188-211 Front Matter....Pages 213-213 A Colony-Forming Assay for Experimental Tumours Using the Plasma Clot Diffusion Chamber....Pages 215-223 Long-Term Culture of Monoclonal Human Tumour Cells in Diffusion Chambers....Pages 224-233 The Use of Diffusion Chambers in the Study of Tumour Immunobiology and Lymphokines....Pages 234-241 Front Matter....Pages 213-213 Differentiation into Granulopoiesis in Human Acute Leukemia and Blast Crisis in Chronic Myelocytic Leukemia....Pages 242-250 Isolated Leukemic Cell Influence in Normal Hemic Colony Growth....Pages 251-258 Erythropoiesis in Plasma Clot Diffusion Chamber (PCDC) Culture....Pages 259-262 Summary and Conclusions....Pages 263-268 Back Matter....Pages 269-277 Proceedings of a Conference on Application of Diffusion Chamber Culture in Study of Hemopoiesis, Clonal Growth of Tumors, and Cytogenetic and Carcinogenic Effects of Chemicals, Held at Brookhaven National Laboratory, Upton, NYfrom 25 - 29 June 1979