Detecting Pathogens in Food (Woodhead Publishing Series in Food Science, Technology and Nutrition)

Identifying pathogens in food quickly and accurately is one of the most important requirements in food processing. The ideal detection method needs to combine such qualities as sensitivity, specificity, speed and suitability for on-line applications. Detecting pathogens in food brings together a distinguished international team of contributors to review the latest techniques in microbiological analysis and how they can best be used to ensure food safety. Part one looks at general issues, beginning with a review of the role of microbiological analysis in food safety management. There are also chapters on the critical issues of what to sample and how samples should be prepared to make analysis effective, as well as how to validate individual detection techniques and assure the quality of analytical laboratories. Part two discusses the range of detection techniques now available, beginning with traditional culture methods. There are chapters on electrical methods, ATP bioluminescence, microscopy techniques and the wide range of immunological methods such as ELISAs. Two chapters look at the exciting developments in genetic techniques, the use of biosensors and applied systematics. Detecting pathogens in food is a standard reference for all those concerned in ensuring the safety of food. Reviews the latest techniques in microbiological analysis and how they can best be used to ensure food safety Examines the role of microbiological analysis in food safety management and discusses the range of detection techniques available Includes chapters on electrical methods, ATP bioluminescence, microscopy techniques and immunological methods such as ELISAs Identifying pathogens in food quickly and accurately is one of the most important requirements in food processing. The ideal detection method needs to combine such qualities as sensitivity, specificity, speed and suitability for on-line applications. Detecting pathogens in food brings together a distinguished international team of contributors to review the latest techniques in microbiological analysis and how they can best be used to ensure food safety.

Part one looks at general issues, beginning with a review of the role of microbiological analysis in food safety management. There are also chapters on the critical issues of what to sample and how samples should be prepared to make analysis effective, as well as how to validate individual detection techniques and assure the quality of analytical laboratories. Part two discusses the range of detection techniques now available, beginning with traditional culture methods. There are chapters on electrical methods, ATP bioluminescence, microscopy techniques and the wide range of immunological methods such as ELISAs. Two chapters look at the exciting developments in genetic techniques, the use of biosensors and applied systematics.

Detecting pathogens in food is a standard reference for all those concerned in ensuring the safety of food.

• Reviews the latest techniques in microbiological analysis and how they can best be used to ensure food safety
• Examines the role of microbiological analysis in food safety management and discusses the range of detection techniques available
• Includes chapters on electrical methods, ATP bioluminescence, microscopy techniques and immunological methods such as ELISAs

Content: Related titles from Woodhead's food science, technology and nutrition list , Page ii Front Matter , Page iii Copyright , Page iv Contributor contact details , Pages xi-xiv Introduction , Pages xv-xix , T. McMeekin 1 - Microbiological analysis and food safety management: GMP and HACCP systems , Pages 3-19 , C. de W. Blackburn 2 - Sampling techniques , Pages 20-51 , D. Legan, M.H. Vandeven 3 - Separation and concentration of samples , Pages 52-68 , A.N. Sharpe 4 - Validating detection techniques , Pages 69-92 , J. Debevere, M. Uyttendale 5 - Quality assurance of laboratory performance , Pages 93-119 , R. Wood, J.E.L. Corry 6 - Culture methods , Pages 123-146 , P. Stephens 7 - Electrical methods , Pages 147-164 , D. Gibson 8 - ATP bioluminescence , Pages 165-185 , M. Griffiths, L. Brovko 9 - Microscopy techniques: DEFT and flow cytometry , Pages 186-216 , R. Raybourne, M. Tortorello 10 - Immunological techniques: immunochromotography, enzyme-linked immonoflourescent assays and agglunination techniques , Pages 217-240 , C.L. Baylis 11 - Immunological techniques: ELISA , Pages 241-258 , J. McCarthy 12 - Genetic techniques: PCR, NASBA, hybridisation and microarrays , Pages 259-270 , K. Sanderson, D. Nichols 13 - Genetic techniques: molecular subtyping methods , Pages 271-293 , C. Fitzgerald, B. Swaminathan, A. Sails 14 - New biosensors for microbiological analysis of food , Pages 294-331 , G. Volpe, G. Palleschi, A.P.F. Turner 15 - The use of applied systematics to identify foodborne pathogens , Pages 332-359 , M. Uyttendaele, J. Debevere Index , Pages 360-370 Introduction / -- T. McMeekin -- PART I. GENERAL ISSUES Microbiological analysis and food safety management: GMP and HACCP systems -- C. de W. Blackburn Sampling techniques -- D. Legan, -- M.H. Vandeven Separation and concentration of samples -- A. Sharpe Validating detection techniques -- J. Debevere, -- M. Uyttendaele Quality assurance of laboratory performance -- R. Wood, -- J.E.L. Corry -- PART II. PARTICULAR TECHNIQUES Culture methods -- P. Stephens Electrical methods -- D.M. Gibson ATP bioluminescence -- M. Griffiths, -- L. Brovko Microscopy techniques: DEFT and flow cytometry -- R. Raybourne, -- M. Tortorello Immunological techniques: immunochromatography, enzyme linked immunofluorescent assays and agglutination techniques -- C.L. Baylis Immunological techniqeus: ELISA -- Genetic techniques: PCR, NASBA, hybridisation and microarrays -- K. Sanderson, -- D. Nichols Genetic techniques: molecular subtyping methods -- C. Fitzgerald, -- B. Swaminathan New biosensors for microbiological analysis of food -- G. Volpe, -- G. Palleschi The use of applied systematics to identify foodborne pathogens -- M. Uyttendaele, -- J. Debevere.

detecting Pathogens In Food Brings Together A Distinguished International Team Of Contributors To Review The Latest Techniques In Microbiological Analysis And How They Can Best Be Used To Ensure Food Safety. The First Part Of The Book Deals With General Issues, Such As The Role Of Microbiological Analysis In Food Safety Management, As Well As The Critical Issues Of What To Sample And How Samples Should Be Prepared To Make Analysis Effective. The Second Section Discusses The Range Of Detection Techniques Now Available, Including Exciting Developments In The Use Of Biosensors And Applied Systematics.