Dendritic Cells: Biophysics, Tumor Microenvironment and Chinese Traditional Medicine (SpringerBriefs in Biochemistry and Molecular Biology)

In this book, the authors discuss the biophysical characteristics of Dendritic cells (DCs) at various differentiation stages and in tumor microenvironments in detail. A type of Chinese traditional herb, which can improve the biophysical and microrheological properties of DCs, is also introduced. DCs are potent and specialized antigen-presenting cells that play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The biophysical and microrheological characteristics of DCs are important for their motility and normal immune function. In tumor microenvironments, the motility and immune functions of DCs are impaired, but the mechanisms are largely unknown. This book offers researchers a comprehensive and deep insight into the biophysical characteristics of DCs and may also help to improve the efficiency of anti-tumor therapy based on DCs. Contents 6 Abstract 7 1 Introduction 8 Abstract 8 References 10 2 The Measurement Protocols for Cell's Biophysical Characteristics 12 Abstract 12 2.1 Isolation of Monocytes (MOs) and Generation of DCs 12 2.2 Fluorescence Polarization 13 2.3 Cell Osmotic Fragility 13 2.4 Cell Deformcapability 13 2.5 Flow Chamber Assay 15 2.6 Cell Electrophoretic Mobility 16 2.7 Fourier-Transformed Infrared Spectra 16 2.8 Confocal Laser Scanning Microscopy Analysis 17 References 17 3 Biophysical Characteristics of DCs at Different Differentiation Stages 18 Abstract 18 References 29 4 Biophysical Characteristics of DCs in Tumor Microenvironment 31 Abstract 31 References 57 5 Effects of Traditional Chinese Medicine on DCs Under Tumor Microenvironment 60 Abstract 60 References 66 6 Future Perspectives 69 Abstract 69 Acknowledgments 70 Front Matter....Pages i-vii Introduction....Pages 1-4 The Measurement Protocols for Cell’s Biophysical Characteristics....Pages 5-10 Biophysical Characteristics of DCs at Different Differentiation Stages....Pages 11-23 Biophysical Characteristics of DCs in Tumor Microenvironment....Pages 25-53 Effects of Traditional Chinese Medicine on DCs Under Tumor Microenvironment....Pages 55-63 Future Perspectives....Pages 65-66 Introduction.-℗lThe measurement protocols of cell biophysical characteristics -- Biophysical characteristics of dendritic cells at different differentiation stages -- Biophysical characteristics of dendritic cells in tumor microenvironment -- Effects of Chinese traditional herbs on dendritic cells -- Future perspectives Introduction.- The measurement protocols of cell biophysical characteristics -- Biophysical characteristics of dendritic cells at different differentiation stages -- Biophysical characteristics of dendritic cells in tumor microenvironment -- Effects of Chinese traditional herbs on dendritic cells -- Future perspectives