Cyanidioschyzon Merolae : A New Model Eukaryote for Cell and Organelle Biology

This comprehensive book highlights the importance of __Cyanidioschyzon merolae__ (__C. merolae__), an ultrasmall unicellular red alga, as a model eukaryote organism. The chapters introduce recent studies on __C. merolae__, from culture, synchronization and isolation methods of nucleic acids, proteins and organelles for molecular biological and cytological analyses, as well as its application in genetic engineering of environmental-stress-tolerant crops and oil production. In addition to discussing recent advances based on the complete genome information and molecular biological techniques such as genetic modifications and bioinformatics, the book includes visualization aids demonstrating that both classical and recent imaging techniques of fluorescent and electron microscopy can be applied to analyses of __C. merolae__. This publication offers a definitive resource for both beginners and professionals studying __C. merolae__, particularly in t he field of molecular biology, evolutionary biology, morphology, biochemistry and cell biology, as well as those interested in its applications in medical sciences and agriculture. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Cyanidiales: Evolution and Habitats (Shinya Miyagishima, Jong Lin Wei, Hisayoshi Nozaki, Shunsuke Hirooka)....Pages 3-15 Cellular Structure of Cyanidioschyzon merolae: A Minimum Set of Organelles (Yuuta Imoto, Yamato Yoshida)....Pages 17-27 Front Matter ....Pages 29-29 Procedures for Cultivation, Observation, and Conventional Experiments in Cyanidioschyzon merolae (Shinya Miyagishima, Jong Lin Wei)....Pages 31-41 Isolation of Dividing Organelles from Cyanidioschyzon merolae Cells (Yamato Yoshida, Yuuta Imoto, Fumi Yagisawa)....Pages 43-57 Front Matter ....Pages 59-59 The 100%-Complete Nuclear and Organellar Genome Sequences of the Ultrasmall Red Algal Species Cyanidioschyzon merolae 10D (Hisayoshi Nozaki, Yu Kanesaki, Motomichi Matsuzaki, Shunsuke Hirooka)....Pages 61-72 “Omics” Analyses of Cyanidioschyzon merolae Based on Complete Genome Information (Masaki Yoshida, Takayuki Fujiwara)....Pages 73-85 Procedures for Transformation and Their Applications in Cyanidioschyzon merolae (Takayuki Fujiwara, Mio Ohnuma)....Pages 87-103 Front Matter ....Pages 105-105 Basic Techniques in Fluorescence and Electron Microscopy (Haruko Kuroiwa)....Pages 107-127 Cytological Analyses by Advanced Electron Microscopy (Takako M. Ichinose, Atsuko H. Iwane)....Pages 129-151 Coherent X-ray Diffraction Imaging of Cyanidioschyzon merolae (Yuki Sekiguchi, Amane Kobayashi, Yuki Takayama, Mao Oide, Asahi Fukuda, Takahiro Yamamoto et al.)....Pages 153-173 Front Matter ....Pages 175-175 Cell Cycle and Organelle Division Cycle in Cyanidioschyzon merolae (Takayuki Fujiwara)....Pages 177-185 Regulation of Cell Cycle Progression by Circadian Rhythms in Cyanidioschyzon merolae (Shinya Miyagishima)....Pages 187-194 Control of Cell Nuclear DNA Replication by Chloroplast and Mitochondrion (Yuki Kobayashi, Yu Kanesaki, Mitsumasa Hanaoka, Kan Tanaka)....Pages 195-204 Double-Membrane-Bounded Organelles: Recent Findings Regarding Division, Inheritance, Structure, and Evolution of the Nucleus, Mitochondria, and Chloroplasts (Yamato Yoshida, Yuki Sakamoto, Katsuya Iwasaki, Shinichiro Maruyama, Sachihiro Matsunaga)....Pages 205-233 Single-Membrane-Bound Organelles: Division and Inheritance (Fumi Yagisawa, Yuuta Imoto, Takayuki Fujiwara, Shinya Miyagishima)....Pages 235-249 Regulation of Organelle and Cell Division by Cytoskeletal and Motor Proteins in Cyanidioschyzon merolae (Yamato Yoshida, Yuuta Imoto)....Pages 251-260 Front Matter ....Pages 261-261 Photosynthesis (Naoki Sato, Takashi Moriyama)....Pages 263-281 Nitrogen Metabolism (Sousuke Imamura, Kan Tanaka)....Pages 283-296 Carbon Metabolism (Takashi Moriyama, Natsumi Mori, Naoki Sato)....Pages 297-321 Front Matter ....Pages 323-323 Application of the Tolerance to Extreme Environment to Land Plants (Osami Misumi, Tsuneyoshi Kuroiwa, Shunsuke Hirooka)....Pages 325-341 Metabolic Engineering of Cyanidioschyzon merolae (Nobuko Sumiya, Shinya Miyagishima)....Pages 343-354 Back Matter ....Pages 355-365