Current research in protein chemistry. Techniques, structure, and function. (3. annual symposium of the Protein Society, held ... in Seattle on July 29 - August 2, 1989.) Ed. by Joseph J. Villafranca

Current Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful. Content: Front Matter , Page iii Copyright , Page iv Foreword , Page xiii Preface , Page xv Dedication to Emil T. Kaiser and Irving S. Sigal , Pages xvii-xxv Acknowledgments , Page xxvii 1 - A RAPID AND NOVEL MEANS OF PROTEIN HYDROLYSIS BY MICROWAVE IRRADIATION USING TEFLON-PYREX TUBES , Pages 3-10 2 - Analysis of Amino Acids by High Performance Capillary Electrophoresis , Pages 11-22 3 - An Evaluation of Microwave Heating for the Vapor Phase Hydrolysis of Proteins: I. Comparison to Vapor Phase Hydrolysis for 24 Hours , Pages 23-36 4 - PERFORMANCE EVALUATION AUTOMATIC HYDROLYSIS AND PTC AMINO ACID ANALYSIS , Pages 37-48 5 - A COLLABORATIVE AMINO ACID ANALYSIS STUDY FROM THE ASSOCIATION OF BIOMOLECULAR RESOURCE FACILITIES , Pages 49-61 6 - RECENT DEVELOPMENTS IN SOLID PHASE PEPTIDE SYNTHESIS USING THE 9-FLUORENYLMETHYOXYCARBONYL (FMOC) PROTECTING GROUP STRATEGY , Pages 63-77 7 - RELATIONSHIPS OF PARAMECIUM AND ENDOCRINE SECRETORY PROTEINS , Pages 79-85 8 - Quantitative Electrotransfer of Proteins From Polyacrylamide Gels onto PVDF Membranes , Pages 87-93 9 - Selective Isolation of Disulfide-Containing Peptides from Trypsin Digests Using Strong Cation Exchange HPLC , Pages 95-102 10 - ANALYSIS OF PEPTIDES AND PROTEINS BY TANDEM QUADRUPOLE MASS SPECTROMETRY , Pages 105-116 11 - PROTEIN AND OLIGOPEPTIDE SEQUENCE ANALYSIS ON THE TSQ-70 TRIPLE QUADRUPOLE MASS SPECTROMETER , Pages 117-126 12 - Sequence Analysis Of Peptides By Plasma Desorption Mass Spectrometry , Pages 127-138 13 - Study of the Primary Structure of Recombinant Proteins with a Benchtop Gas Chromatograph-Mass Spectrometer-Computer Data System , Pages 139-149 14 - Synthesis and Mass Spectrometry of Phosphotyrosine-Containing Peptides , Pages 151-158 15 - Design, Characterization and Results of ABRF-89SEQ: A Test Sample For Evaluating Protein Sequencer Performance in Protein Microchemistry Core Facilities , Pages 159-166 16 - Extended N-Terminal Protein Sequencing of Clostridium botulinum Neurotoxin Type A Fragments: Continuous Sequence to Seventy Residues Both from Glass-Fiber Filter Using the Gas Phase and Polyvinylidene Difluoride Using the Pulsed Liquid Phase Sequencer , Pages 167-178 17 - PEPTIDE MAPPING AND INTERNAL SEQUENCING OF PROTEINS FROM ACRYLAMIDE GELS , Pages 179-190 18 - New Approaches to Covalent Sequence Analysis , Pages 191-202 19 - ANALYSIS OF BRAIN CONSTITUENTS BY CAPILLARY ELECTROPHORESIS , Pages 203-216 20 - Capillary Electrophoresis Analysis of Species Variations in the Tryptic Maps of Cytochrome C , Pages 217-232 21 - ULTRAFAST PROTEIN ANALYSIS: A POWERFUL TECHNIQUE FOR RECOMBINANT PROTEIN PROCESS MONITORING , Pages 233-244 22 - Separation and Characterization of Proteins in the Range of 1 to 200 kDa with HPECTM , Pages 245-262 23 - Protein Detection During Polyacrylamide Gel Electrophoresis Using High Resolution Dynamic Imaging , Pages 263-269 24 - DESIGN, COMPUTER DERIVED STRUCTURE AND BIOLOGICAL ACTIVITY OF THREE BICYCLIC GONADOTROPIN RELEASING HORMONE (GnRH) ANTAGONISTS , Pages 273-281 25 - THE H-HELIX OF MYOGLOBIN AS A POTENTIAL INDEPENDENT PROTEIN FOLDING DOMAIN , Pages 283-293 26 - Probing the Role of Position 49 in the Folding of the Alpha Subunit of Tryptophan Synthase from Escherichia Coli , Pages 295-300 27 - Tests Of Isolated β–Sheet Formation In A Cyclic Peptide From BPTI , Pages 301-308 28 - Spectroscopic and Chromatographic Studies of Native and Denatured States of T4 Lysozymes , Pages 309-322 29 - Studies of the Alkaline Denaturation of Hemoglobin in an NMR Optical Stopped-Flow Apparatus , Pages 323-329 30 - CONFORMATIONAL STUDIES OF PEPTIDES CORRESPONDING TO THE LDL RECEPTOR CYTOPLASMIC TAIL AND TRANSMEMBRANE DOMAIN , Pages 331-338 31 - Biochemical and Biophysical Comparison of Bacterial DnaK and Mammalian hsc73, Two Members of an Ancient Stress Protein Family , Pages 339-347 32 - The Crystal Structure of Human Interleukin-1α , Pages 351-358 33 - Crystal Structure of a Protein-Toxin Solved from a Predicted Model , Pages 359-366 34 - Segmental Accommodation: A Novel Conformational Change Induced Upon Ligand Binding by Thymidylate Synthase , Pages 367-382 35 - Subunit Interactions and the Function of Tumor Necrosis Factor , Pages 383-394 36 - STRUCTURE PREDICTION METHODS FOR MEMBRANE PROTEINS: COMPARISON WITH THE X-RAY STRUCTURE OF THE R. VIRIDIS PHOTOSYNTHETIC REACTION CENTRE , Pages 395-404 37 - THE SEQUENCE ATTRIBUTES METHOD FOR DETERMINING CORRELATIONS BETWEEN AMINO ACID SEQUENCE AND PROTEIN SECONDARY STRUCTURE , Pages 405-415 38 - Acid-Denatured States of Proteins , Pages 417-424 39 - ESTIMATION OF PROTEIN STABILITY COMPARISON OF CALORIMETRIC AND DENATURANT STUDIES , Pages 425-434 40 - ENGINEERING OF MULTIPLE DISULFIDE BONDS DRAMATICALLY STABILIZES T4 LYSOZYME , Pages 435-447 41 - E. coli Thioredoxin Stability Is Greatly Enhanced by Substitution of Aspartic Acid 26 by Alanine , Pages 449-456 42 - MEASUREMENTS OF DISULFIDE BOND STABILITIES IN PROTEIN FOLDING INTERMEDIATES , Pages 457-466 43 - SOLUBILITY OF RECOMBINANT PROTEINS EXPRESSED IN ESCHERICHIA COLI , Pages 467-478 44 - MULTIPLE DETERMINANTS FOR SUBSTRATE SPECIFICITIES IN CYTOCHROME P450 ISOZYMES , Pages 481-488 45 - Catalysis by Chimeric Proteins: Conversion of a β-Iactamase to a D,D-Carboxypeptidase , Pages 489-497 46 - Molecular Mapping of Immunogenic Determinants of Human CD4 Using Chimeric Interspecies Molecules and Anti-CD4 Antibodies , Pages 499-508 47 - EETI II ( Ecballium elaterium Trypsin Inhibitor II) as a Vehicle for New Functions , Pages 509-519 48 - Comparison of the Dimerization, Stability and Inhibitor Binding Between HIV-1 Protease Wild Type and Active Site Mutant , Pages 521-528 49 - STRUCTURE / FUNCTION ANALYSIS OF IL-2-TOXIN: FRAGMENT B REQUIREMENTS FOR THE TRANSLOCATION OF FRAGMENT A INTO THE CYTOSOL OF TARGET CELLS , Pages 529-540 50 - ALTERATION OF THE SITE OF ADENYLYLATION OF GLUTAMINE SYNTHETASE BY SITE-DIRECTED MUTAGENESIS , Pages 541-548 51 - Three-Dimensional Protein Structure Prediction Workshop: Overview and Summary , Pages 551-556 52 - PROTEIN STRUCTURE PREDICTION , Pages 557-565 53 - Knowledge-Based Protein Modelling: Human Plasma Kallikrein and Human Neutrophil Defensin , Pages 567-574 54 - Application of a Knowledge Based Strategy to the Prediction of Protein Tertiary Structure , Pages 575-582 55 - Structure Determination of Haloalkane Dehalogenase , Pages 583-587 Index , Pages 589-606 Current Research in Protein Chemistry: Techniques, Structure, and Function reviews new techniques and methods for determining the structure and function of proteins. Topics covered include protein folding and stability, chimeric proteins, amino acid and peptide analysis, mass spectrometric methods, and protein sequencing techniques. This book is divided into six sections comprised of 55 chapters. The discussion begins with a description of microwave irradiation that uses Teflon-Pyrex tubes for protein hydrolysis, followed by the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow explore mass spectrometry, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. Chapters focus on the crystal structure of human interleukin-1a, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins. This book will be of value to students and researchers interested in protein chemistry.