Current Issues in Plant Molecular and Cellular Biology : Proceedings of the VIIIth International Congress on Plant Tissue and Cell Culture, Florence, Italy, 12–17 June, 1994

Plant cell and tissue culture is a relevant area of experimental biology that has been developed for some decades to become an indispensable tool of plant biotechnology. Progress in this area, sometimes tumultuous, has been regularly recorded by the proceedings of the congresses of the International Association for Plant Tissue Culture which have been held every four years in several continents. This book reports plenary lectures, keynote lectures and invited oral presentations given at the last congress held in Florence. It is a useful reference guide both for established scientists and students on both traditional and emerging fields of plant biology. The following topics are covered: __In vitro__ Culture and Plant Regeneration; Plant Propagation; Haploids; Somatic Hybridisation; Reproductive Systems; Genetic Variability; Gene Transfer; Organelles; Biotechnology of Tropical and Subtropical Species; Agronomic Traits; Somatic Embryogenesis; Meristems; Cell Surface; Growth Regulators; Reception and Transduction of Signals; Gene Expression under Extreme Conditions; Primary Metabolism; Secondary Metabolism; Transport; Large Scale Production. Front Matter....Pages i-xv International Programmes of FAO and IAEA in Mutation Breeding and in Vitro Technology....Pages 1-3 Cellular and Molecular Genetic Improvement of Cereals....Pages 5-18 Plasma Membrane-Cell-Wall Adhesion and its Role in Response to Biotic and Abiotic Stresses....Pages 19-29 Molecular and Cellular Events in the Formation of New Meristems....Pages 31-35 Oligosaccharide Elicitors and Elicitor Receptors....Pages 37-58 In Vitro Culture and Plant Regeneration in Gramineous Crops....Pages 59-68 Protoplast, Callus and Suspension Culture of Perennial Ryegrass — Effect of Genotype and Culture System....Pages 69-74 Breakthrough in the Plant Regeneration from Protoplasts of Vicia Faba and V. Narbonensis ....Pages 75-80 Plant Regeneration from Suspension and Protoplast Cultures in the Temperate Grasses Festuca and Lolium ....Pages 81-86 In Vitro Organogenesis, Transformation and Expression of Drought-Related Proteins in Forest Tree Cultures....Pages 87-94 Evolution and Automation in Micropropagation and Artificial Seed Production....Pages 95-104 A New Concept of Plant In Vitro Cultivation Liquid Medium: Temporary Immersion....Pages 105-110 Hormone Requirements During the Successive Phases of Rooting of Malus Microcuttings....Pages 111-116 Propagation of Magnolia and Uriodendron Via Somatic Embryogenesis....Pages 117-122 Tuberization in Vitro and Dormancy of Potato (Solanum Tuberosum L.) Microtubers....Pages 123-125 Haploids — an Integral Part of Applied and Basic Research....Pages 127-135 Utilization of Anther Culture as a Breeding Tool in Rice Improvement....Pages 137-142 Application of Haploidy in Genetic Manipulation of Canola....Pages 143-148 Dihaploid Chicory ( cichorium Intybus L.) Via Microspore Culture....Pages 149-154 Use of Anther Culture Method in Pepper Breeding: Factors Affecting Plantlets Production....Pages 155-160 Protoplast-Fusion Derived Cybrids in Solanaceae....Pages 161-169 Brassica Oleracea Cybrids for Hybrid Vegetable Production....Pages 171-176 Recovery of Citrus Somatic Hybrids Tolerant to Phoma Tracheiphila Toxin, Combining Selection and Identification by RAPD Markers....Pages 177-183 Towards producing bloat-safe Medicago sativa L. through protoplast fusion....Pages 185-190 Self and Cross Pollination of Ovules in Test Tubes....Pages 191-199 Embryogenesis and Plant Regeneration from in Vitro Fused Isolated Gametes of Maize....Pages 201-206 Fertile Barley Plants can be Regenerated from Mechanically Isolated Protoplasts of Fertilized Egg Cells....Pages 207-212 Experiments on Inter-Genera and Inter-Families Pollen Grains Protoplast Fusion....Pages 213-218 Mitochondrial Regulation of Petal and Stamen Development in Cytoplasmic Male Sterile Cultivars of Nicotiana Tabacum....Pages 219-224 Exploiting Somaclonal Variation — Especially GENE Introgression from Alien Chromosomes....Pages 225-234 Acetohydroxyacid Synthase GENE Amplification Induces Clorsulfuron Resistance in Daucus Carota L.....Pages 235-240 RAPD Analysis of Sugarcane During Tissue Culture....Pages 241-246 Evidence of Somaclonal Variation in Somatic Embryo-Derived Plantlets of White Spruce ( Picea Glauca (Moench) Voss....Pages 247-252 GENE Technology for Developing Countries: Genetic Engineering of Indica Rice....Pages 253-262 Production of Transgenic Cereal Crops....Pages 263-269 The Embryo as a Tool for Genetic Engineering in Higher Plants....Pages 271-277 Production of Fertile Transgenic Maize Plants by Silicon Carbide Whisker-Mediated Transformation....Pages 279-284 Studies on the Stability of Foreign Genes in the Progeny of Transgenic Lines of Vicia Narbonensis ....Pages 285-290 Molecular Analysis of Chloroplast Division....Pages 291-296 Integration of Solanum DNA into the Nicotiana Plastome Through Peg-Mediated Transformation of Protoplasts....Pages 297-301 Physical Maps of Mitochondrial Genomes from Male-Fertile and Male-Sterile Sugar Beets....Pages 303-308 A tRNA Gene Mapping Within the Chloroplast rDNA Cluster is Differentially Expressed During the Development of Daucus carota....Pages 309-314 Cryopreservation of Several Tropical Plant Species using Encapsulation/Dehydration of Apices....Pages 315-320 Application of Biotechnology to Carica Papaya and Related Species....Pages 321-326 Towards the Production of Transgenic Tropical Maize Germplasm with Enhanced Insect Resistance....Pages 327-331 DNA-Based Chromosome Markers: Brassica Crops as Case Study....Pages 333-339 Application of DNA Amplification Fingerprinting in the Breeding of Phalaenopsis Orchid....Pages 341-346 Maintenance of an in Vitro Collection of Allium in the Gatersleben Genebank — Problems and Use....Pages 347-352 In Vitro Selection of a Salt-Tolerant Cell Line from PEA Calli Cultures....Pages 353-358 The Role of Secreted Proteins in Carrot Somatic Embryogenesis....Pages 359-368 Expression of Floral Specific Genes in Tomato Hypocotyls in Liquid Culture....Pages 369-375 Regeneration of Plants from Protoplasts of Arabidopsis Thaliana CV. Columbia L. (C24), Via Direct Embryogenesis....Pages 377-382 Controlling Factors and Markers for Embryogenic Potential and Regeneration Capacity in Barley ( Hordeum Vulgare L.) Cell Cultures....Pages 383-388 Importance of Extracellular Proteins for Somatic Embryogenesis in Picea Abies....Pages 389-392 Nutrient Absorption and The Development and Genetic Stability of Cultured Meristems....Pages 393-403 Multiple Floral Bud Formation In Vitro on the Giant Dome of Eleusine Coracana Gaertn....Pages 405-410 Effect of Gibberellic Acid (GA 3 ) and Micropropagation on Axillary Shoot Induction in Monostem Genotype (To-2) of Tomato (L. Esculentum Mill)....Pages 411-416 The Plant Oncogene Rolb Enhances Meristem Formation in Tobacco thin Cell Layers....Pages 417-422 Results, Showing Possibilities of Meristem Method for Improving Some Characteristics of Potato Varieties....Pages 423-426 Sieve Elements Isolated from Callus Cultures Can be used to Raise Phloem-Specific Monoclonal Antibodies....Pages 427-432 Oligosaccharides from Xyloglucan Affect the Development of Rubus Fruticosus Cell Suspension Culture....Pages 433-443 Rapid Wall Surface Rearrangements Induced by Oligosaccharides in Suspension-Cultured Cells....Pages 445-455 Cell Wall Break Down and Enhancement of Cellulase Activity During the Emergence of Callus from Rice Roots in the Presence Of 2,4-D....Pages 457-462 Auxin Perception at the Plasma Membrane of Plant Cells: Recent Developments and Large Unknowns....Pages 463-472 Perception of the Auxin Signal in Carrot Cell Membranes: ABPs, Hormone-Conjugate Processing and Auxin-Regulated Enzyme Activities....Pages 473-479 Endogenous Levels of Four Plant Hormones may Affect the Culture Conditions of Poplar Protoplasts to Regenerate Plants....Pages 481-486 Auxin and Ethylene in Hairy Root Cultures of Hyoscyamus muticus ....Pages 487-492 Effects of Phytohormones on Lateral Root Differentiation in Eucalyptus globulus and Transgenic Nicotiana tabacum Seedlings....Pages 493-499 Theoretical and Experimental Definition of Minimal Photoresponsive Elements in cab and rbcS genes....Pages 501-511 T-DNA Tagging of a Gene Inducing Desiccation Tolerance in Craterostigma plantagineum ....Pages 513-518 Are Arrestin-Like Proteins Involved in Plant Signal Transduction Pathways?....Pages 519-524 An Alternative Approach Towards Understanding Monocot Zygotic Embryogenesis....Pages 525-530 Mutations Conferring Early Flowering on a Late-Flowering Ecotype Of Arabidopsis thaliana ....Pages 531-538 Strategies for Increasing Tolerance Against Low Temperature Stress by Genetic Engineering of Membrane Lipids....Pages 539-544 Aluminum Effects on In Vitro Tissue Cultures of Phaseolus vulgaris ....Pages 545-549 Effect of Hyperosmotic 3-O-methyl-D-Glucose in the Medium on Metabolic Parameters in Actinidia deliciosa Callus....Pages 551-556 Gene Expression in Photosynthetically Active Tomato Cell Cultures is Influenced by Potato Spindle Tuber Viroid Infection....Pages 557-562 Molecular Analysis of the Nitrate Assimilatory Pathway in Solanaceous Species....Pages 563-570 Inheritance of 5-Methyltryptophan-Resistance, Selected In Vitro, In Tobacco and Datura innoxia Plants....Pages 571-576 Molybdenum Cofactor (Nitrate Reductase) Biosynthesis in Plants: First Molecular Analysis....Pages 577-582 Analysis of the Structure of the 5′ End of the Gene Coding for Carrot Dihydrofolate Reductase-Thymidylate Synthase....Pages 583-588 Glutamine Synthetase in Cells from Carrot ( Daucus carota L.): Interaction between Phosphinothricin and Glutamate....Pages 589-594 Changes of Secondary Metabolism by Elicitor Treatment in Pueraria lobata Cell Cultures....Pages 595-604 Expression of Hyoscyamine 6ß-Hydroxylase in Plants and in E. coli ....Pages 605-609 Plant Cell Culture Combined with Chemistry — Potentially Powerful Routes to Clinically Important Compounds....Pages 611-616 Molecular and Metabolic Control of Secondary Metabolism in Tagetes ....Pages 617-622 Aquaporins: Water Channel Proteins in the Plasma Membrane and the Tonoplast....Pages 623-631 The Effect of Brefeldin a on the Golgi Apparatus in Norway Spruce Cells....Pages 633-638 Brefeldin A differentially affects protein secretion from suspension cultured tobacco cells ( Nico-tiana tabacum L.)....Pages 639-645 A Tonoplast Intrinsic Protein, α-TIP, is Present in Seeds and Somatic Embryos of Norway Spruce ( Picea abies )....Pages 647-652 The Characteristics of Atropine Metabolism in Cultured Tissues and Seedlings of Duboisia ....Pages 653-658 Environmental Control for Large Scale Production of In Vitro Plantlets....Pages 659-667 Large Scale Production of Secondary Metabolites....Pages 669-674 SOMES2: Image-Analysis-Based Somatic Embryo Sorter....Pages 675-680 Nutritional Aspects of Daucus Carota Somatic Embryo Cultures Performed in Bioreactors....Pages 681-687 Back Matter....Pages 689-700