Conn's Biological Stains : A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine

Ch 1. Introduction to dyes and stains, F.H. Kasen. Ch 2. The history of staining, B. Bracegirdle. Ch 3. Nomenclature and classification of dyes and other coloring agents, J.A. Kiernan. Ch 4. Applications of dyes, fluorochromes and pigments, M. Wainwright. Ch 5. Mechanisms of biological staining, R.W. Horobin. Ch 6. Dye purity and dye standardisation for biological staining, H. Lyon. Ch 7. Reactive staining reagents and fluorescent labels, J.C. Stockert. Ch 8. The use of dyes and fluorochromes as indicators, M. Wainwright. Ch 9. Nitroso and nitro dyes, R.W. Horobin. Ch 10. Monoazo dyes, R.W. Horobin. Ch 11. Dis-, tris- and poly azo dyes, R.W. Horobin. Ch 12. Diazonium salts and their reaction products with coupling agents, R.W. Horobin. Ch 13. Tetrazolium salts and formazans, R.W. Horobin. Ch 14. Amino di- and triarylmethane dyes, R.W. Horobin. Ch 15. Hydroxy triarylmethanes, R.W. Horobin. Ch 16. Xanthenes, R.W. Horobin. Ch 17. Acridines and phenanthridines, R.W. Horobin. Ch 18. Azines, R.W. Horobin. Ch 19. Oxazines and related dyes, R.W. Horobin. Ch 20. Thiazines, R.W. Horobin. Ch 21. Romanowsky-Giemsa stains, D.H. Wittekind. Ch 22. Polyene dyes and fluorochromes, R.W. Horobin. Ch 23. Polymethines - 1. Cyanines, oxonols, benzimidazoles, indolenines and azamethines, R.W. Horobin. Ch 24. Polymethine dyes - 2. Styryls, thiazoles, coumarins and flavonoids, R.W. Horobin. Ch 25. Carbonyl dyes including indigoids, anthraquinones and naphthalimides, R.W. Horobin. Ch 26. Phthalocyanines, porphyrins and related aza[18]annulenes, R.W. Horobin. Ch 27. Miscellaneous inorganic and organic substances used as biological stains, J.A. Kiernan and R.W. Horobin. Ch 28. Methods for testing biological stains, D.P. Penney and J.M. Powers with the assistance of C. Willis, M. Frank and C. Churukian.;Published on behalf of the Biological Stain CommissionFor 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application.This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications. Cover......Page 1 Half Title......Page 2 Title Page......Page 4 Copyright Page......Page 5 Table of Contents......Page 6 How to use this book......Page 8 Contributors list......Page 10 Preface......Page 11 Acknowledgements......Page 14 Dedications......Page 16 Chapter 1: Introduction to dyes and stains......Page 18 Chapter 2:The history of staining......Page 32 Chapter 3: Nomenclature and classification of dyes and other coloring agents......Page 40 Chapter 4: Applications of dyes, fluorochromes and pigments......Page 58 Chapter 5: Mechanisms of biological staining......Page 70 Chapter 6: Dye purity and dye standardization for biological staining......Page 84 Chapter 7: Reactive staining reagents and fluorescent labels......Page 94 Chapter 8: The use of dyes and fluorochromes as indicators......Page 106 Chapter 9: Nitroso and nitro dyes......Page 118 Chapter 10: Monoazo dyes......Page 124 Chapter 11: Dis-, tris- and polyazo dyes......Page 142 Chapter 12: Diazonium salts and their reaction products with coupling agents......Page 162 Chapter 13: Tetrazolium salts and formazans......Page 174 Chapter 14: Amino di- and triarylmethane dyes......Page 186 Chapter 15: Hydroxy triarylmethanes......Page 220 Chapter 16: Xanthenes......Page 236 Chapter 17: Acridines and phenanthridines......Page 270 Chapter 18: Azines......Page 284 Chapter 19: Oxazines and related dyes......Page 294 Chapter 20: Thiazines......Page 310 Chapter 21: Romanowsky–Giemsa stains......Page 320 Chapter 22: Polyene dyes and fluorochromes......Page 330 Chapter 23: Polymethine dyes – 1. Cyanines, oxonols, benzimidazoles, indolenines and azamethines......Page 340 Chapter 24: Polymethine dyes – 2. Styryls, thiazoles, coumarins and flavonoids......Page 366 Chapter 25: Carbonyl dyes including indigoids, anthraquinones and naphthalimides......Page 384 Chapter 26: Phthalocyanines, porphyrins and related aza[18]annulenes......Page 396 Chapter 27: Miscellaneous inorganic and organic substances used as biological stains......Page 406 Chapter 28: Methods for testing biological stains......Page 434 Bibliography......Page 444 Index......Page 556 Published on behalf of the Biological Stain Commission For 75 years Conn's Biological Stains has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long awaited tenth edition appears 25 years after R.D. Lillie's ninth and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application. This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalogue literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.