Conditional Mutagenesis: An Approach to Disease Models (Handbook of Experimental Pharmacology (178))

Leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse (part 1) and their application to model human physiology and pathophysiology (part 2). It illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. Finally the book considers the development of new drugs to treat them. Front Matter....Pages I-XI Front Matter....Pages 1-1 Conditional Somatic Mutagenesis in the Mouse Using Site-Specific Recombinases....Pages 3-28 Cre/ loxP -Mediated Chromosome Engineering of the Mouse Genome....Pages 29-48 Tetracycline-Controlled Genetic Switches....Pages 49-72 Novel Gene Switches....Pages 73-105 Improved Embryonic Stem Cell Technologies....Pages 107-128 Gene Trap Mutagenesis....Pages 129-147 RNA Interference in Mice....Pages 149-176 Viral Vectors: A Wide Range of Choices and High Levels of Service....Pages 177-202 Conditional Mutagenesis by Cell-Permeable Proteins: Potential, Limitations and Prospects....Pages 203-232 Front Matter....Pages 233-233 Analysis of Mouse Development with Conditional Mutagenesis....Pages 235-262 Conditional Mouse Models of Cancer....Pages 263-287 Conditional Mutagenesis Reveals Immunological Functions of Widely Expressed Genes: Activation Thresholds, Homeostatic Mechanisms and Disease Models....Pages 289-314 Conditional Transgenesis and Recombination to Study the Molecular Mechanisms of Brain Plasticity and Memory....Pages 315-345 A Novel Conditional Knockout Strategy Applied to Serotonin Receptors....Pages 347-363 Conditional Mouse Models for Friedreich Ataxia, a Neurodegenerative Disorder Associating Cardiomyopathy....Pages 365-375 Animal Models in Cardiovascular Diseases: New Insights from Conditional Models....Pages 377-405 Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders....Pages 407-439 Conditional Mouse Models to Study Developmental and Pathophysiological Gene Function in Muscle....Pages 441-468 Analysis of Calcium Channels by Conditional Mutagenesis....Pages 469-490 Conditional Mutagenesis of G-Protein Coupled Receptors and G-Proteins....Pages 491-509 Front Matter....Pages 233-233 Contribution of Targeted Conditional Somatic Mutagenesis to Deciphering Retinoid X Receptor Functions and to Generating Mouse Models of Human Diseases....Pages 511-524 Back Matter....Pages 525-529 Conditional somatic mutagenesis in the mouse using site-specific recombinases Cre/loxP-mediated chromosome engineering of the mouse genome Tetracycline-controlled genetic switches Novel gene switches Improved embryonic stem cell technologies Gene trap mutagenesis RNA interference in mice Viral vectors: a wide range of choices and high levels of service Conditional mutagenesis by cell-permeable proteins: potential, limitations and prospects Analysis of mouse development with conditional mutagenesis Conditional mouse models of cancer Conditional mutagenesis reveals immunological functions of widely expressed genes: activation thresholds, homeostatic mechanisms and disease models Conditional transgenesis and recombination to study the molecular mechanisms of brain plasticity and memory A novel conditional knockout strategy applied to serotonin receptors Conditional mouse models for Friedreich Ataxia, a neurodegenerative disorder associating cardiomyopathy Animal models in cardiovascular diseases: new insights from conditional models Conditional animal models for the study of lipid metabolism and lipid disorders Conditional mouse models to study developmental and pathophysiological gene function in muscle Analysis of calcuim channels by conditional mutagenesis Conditional mutagenesis of G-protein coupled receptors and G-proteins Contribution of targeted conditional somatic mutagenesis to deciphering retinoid X receptor functions and to generating mouse models of human diseases. Provides comprehensive information on methods for conditional mutagenesis in the mouse and their application to model human physiology and pathophysiology. This book illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms