Commercial and Pre-commercial Cell Detection Technologies for Defence Against Bioterror : Technology, Market and Society

As a response to the rapidly emerging threat of bioterrorism, the objectives of this volume of Commercial and Pre-Commercial Cell Detection Technologies for Defence against Bioterror are to exchange information on commercially available technologies and equipment for defense against bioterrorism; to further the development of new biosensor system prototypes into a commercially available apparatus and to explore human factors in BWA biosensors. The new commercial and pre-commercial technologies that are currently emerging in the world are presented and explained. Furthermore, there is a discussion about the interaction of modern detection systems with society and a trial for improvement of the relation between the scientific community and commercial entities. There are four major areas highlighted: the first is a presentation of the most advanced biosensors and biodetection system which can be found in the market or are quite close to commercialization. Systems as the BIOHAWK???„??, SASS 2000, RAPTOR, Bionas?‚?® 2500, OWLS, or a portable SPR are presented in this section. The second issue is a presentation of the advances in the research of biodetection devices as DNA and protein microchips, micro and nanophotonic sensors, CMOS microsensor chips, electrochemical arrays, physical platforms, electro optical detection, mass detection, etc. Then, there is a description of the latest developments in the employment of bioreceptor layers for the selective detection of BWA, as protein signatures, molecular imprinted polymers, membrane engineering (MIME), cell signatures, monoclonal antibodies, synthetic antibodies and lytic phages, among others. The last part is the discussion of the human factor: societal issues related to sensor development and employment for BWA detection.IOS Press is an international science, technical and medical publisher of high-quality books for academics, scientists, and professionals in all fields. Some of the areas we publish in: -Biomedicine -Oncology -Artificial intelligence -Databases and information systems -Maritime engineering -Nanotechnology -Geoengineering -All aspects of physics -E-governance -E-commerce -The knowledge economy -Urban studies -Arms control -Understanding and responding to terrorism -Medical informatics -Computer Sciences Cover ......Page 1 Sub-Series E: Human and Societal Dynamics – Vol. 39 ISSN 1874-6276 ......Page 3 Title page......Page 4 Copyright ......Page 5 Preface......Page 6 DMITRI IVNITSKI......Page 7 ANATOLY RESHETILOV......Page 8 JAN KREJ......Page 9 Contents......Page 10 Introduction......Page 12 1.1 Databases......Page 13 1.2 Integrated Multiplexed Sensors......Page 14 1.4 Sensors in the Public and Private Domain......Page 15 Figure 2......Page 16 2. Societal Impact......Page 18 4. Management of Concerns......Page 19 Acknowledgements......Page 20 1. Introduction......Page 21 2.1. Sensor set-up......Page 22 2.2. Immunosensing of chemical pollutants......Page 23 2.3. Analysis of real water samples and multianalyte format......Page 25 2.4. Evaluation in human samples......Page 27 3.1. Sensor development......Page 28 3.2. DNA testing......Page 29 3.3. Lab-on-a-chip integration......Page 30 6. References......Page 31 Introduction......Page 32 Figure 1.......Page 33 Figure 2.......Page 34 Instrument Testing......Page 35 Field Testing Experience......Page 37 Acknowledgements......Page 39 References......Page 40 Introduction......Page 41 1. Screen-Printed Electrodes production......Page 42 2. Screen-Printed Biosensor......Page 43 3. Screen-Printed Biosensor as Alarm system against terrorism......Page 44 References......Page 47 Introduction......Page 49 Mediatorless Biosensor Based On Direct Electron Transfer (DET)......Page 50 Three-Dimensional Multi Microchannel Technology......Page 52 References......Page 54 Electro-Optical Analysis as a Tool for Determination of Microbial Cells with the Help of Specific Bacteriophages......Page 56 Introduction......Page 57 Measurement of the electrorotation spectra (ES)......Page 58 Results and discussion......Page 59 Figure 2......Page 60 Figure 3......Page 61 Acknowledgements......Page 62 References......Page 63 Introduction......Page 65 Method......Page 66 Figure 1......Page 67 Figure 2......Page 68 Conclusion......Page 69 Reference......Page 70 Introduction......Page 71 1.1. The Bulk Acoustic Wave Devices......Page 73 Unknown......Page 0 1.2. The Surface Acoustic Wave Devices......Page 76 2.1. Synthetic Antibodies—Imprinting......Page 77 2.2. Surface Imprinting with Bio-analytes......Page 78 3.1. Yeast Cells......Page 79 3.2. Bacteria......Page 81 3.3.1. Tobacco Mosaic Viruses (TMV)......Page 83 3.3.3. Parapox Ovis Viruses......Page 84 Imprinted......Page 85 References......Page 86 Introduction......Page 88 Conclusion and Outlook......Page 89 References......Page 90 Introduction......Page 91 Energy metabolism......Page 92 Oxidative phosphorylation / Oxygen consumption......Page 93 Cell adhesion and confluence......Page 94 Detection System......Page 95 Cells......Page 97 ATP assay......Page 98 Biological mechanisms of action......Page 99 Dynamic effects of compounds......Page 100 Discussion......Page 106 Conclusions......Page 107 References......Page 108 Introduction......Page 110 Materials.......Page 114 Immobilization of Enzymes and Registration of Enzyme Activities.......Page 115 Detection of Microorganisms on the Basis of Registration of Their Enzymatic Activity......Page 116 Matrix Type Selection......Page 120 Analysis of Enzyme Substrates......Page 121 Characteristics of the Developed Immunoassay......Page 122 Acknowledgements......Page 123 References......Page 124 Molecular Identification Through Membrane-Engineering (MIME): State-of-the-Art Biosensor Technology for Instant, Ultra-Specific and Ultra-Sensitive Detection of Infectious Disease Agents at Global Scale......Page 126 Introduction: the new era of cellular biosensors......Page 127 Bioelectric Recognition Assay (BERA)......Page 128 Systems integration......Page 131 Commercialization efforts......Page 132 Global implementation of BERA/MIME sensors and the future of early warning biodefense systems......Page 133 References......Page 134 Introduction......Page 136 Figure 1......Page 137 Results and discussion......Page 140 Figure 4......Page 138 Acknowledgements......Page 141 References......Page 142 Introduction......Page 143 Experiments......Page 144 Data Analysis......Page 146 Training Set: = Experiment 1 / Test Set = Experiment 2......Page 147 Training Set = Experiment 2 / Test Set = Experiment 1......Page 148 Conclusion......Page 149 References......Page 150 Cross Validation......Page 151 Introduction......Page 152 Glucose Dehydrogenases in Biosensor Design......Page 153 Alcohol Dehydrogenases in Biosensor Design......Page 156 Glycerol Dehydrogenases in Biosensor Design......Page 158 References......Page 159 Introduction......Page 161 Organophosphorous toxic compounds......Page 162 The principle of biosensor nerve agents detection......Page 164 Technical solution of biosensor technology for NA measurement......Page 167 Results and discussion......Page 169 Conclusions......Page 175 Reference......Page 176 Author Index......Page 178 Edited By Laura M. Lechuga ... [et Al.]. Published In Cooperation With Nato Public Diplomacy Division. Proceedings Of The Nato Advanced Research Workshop On A Commercial And Pre-commercial Cell Detection Technologies For Defence Against Bioterror- Technology, Market And Society, Brno, Czech Republic, 3-6 September 2006--t.p. Verso. Includes Bibliographical References And Index. As a response to the rapidly emerging threat of bioterrorism, this volume aims to exchange information on commercially available technologies and equipment for defense against bioterrorism; to further the development of new biosensor system prototypes into a commercially available apparatus and to explore human factors in BWA biosensors.