Circulating Nucleic Acids in Serum and Plasma – CNAPS IX (Advances in Experimental Medicine and Biology Book 924)

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them. Front Matter....Pages i-xxii Front Matter....Pages 1-1 Circulating Cell-Free miR-373, miR-200a, miR-200b and miR-200c in Patients with Epithelial Ovarian Cancer....Pages 3-8 Cell-Free miRNA-141 and miRNA-205 as Prostate Cancer Biomarkers....Pages 9-12 Clinical Utility of Circulating Tumor DNA for Molecular Assessment and Precision Medicine in Pancreatic Cancer....Pages 13-17 An Enquiry Concerning the Characteristics of Cell-Free DNA Released by Cultured Cancer Cells....Pages 19-24 Detection of p53 Mutations in Circulating DNA of Transplanted Hepatocellular Carcinoma Patients as a Biomarker of Tumor Recurrence....Pages 25-28 Unbiased Detection of Somatic Copy Number Aberrations in cfDNA of Lung Cancer Cases and High-Risk Controls with Low Coverage Whole Genome Sequencing....Pages 29-32 Liquid Profiling in Lung Cancer – Quantification of Extracellular miRNAs in Bronchial Lavage....Pages 33-37 Screening of KRAS Mutation in Pre- and Post-Surgery Serum of Patients Suffering from Colon Cancer by COLD-PCR HRM....Pages 39-41 Non-dividing Cell Virtosomes Affect In Vitro and In Vivo Tumour Cell Replication....Pages 43-45 Features of Circulating DNA Fragmentation in Blood of Healthy Females and Breast Cancer Patients....Pages 47-51 Liquid Profiling of Circulating Nucleic Acids as a Novel Tool for the Management of Cancer Patients....Pages 53-60 Front Matter....Pages 61-61 Characterization of Human Pregnancy Specific Glycoprotein (PSG) Gene Copy Number Variations in Pre-eclampsia Patients....Pages 63-65 Non-invasive Prenatal Diagnosis of Feto-Maternal Platelet Incompatibility by Cold High Resolution Melting Analysis....Pages 67-70 Implementing Non-Invasive Prenatal Diagnosis (NIPD) in a National Health Service Laboratory; From Dominant to Recessive Disorders....Pages 71-75 Front Matter....Pages 77-77 Comparative Analysis of Harmful Physical Factors Effect on the Cell Genome....Pages 79-84 Heterochromatic Tandem Repeats in the Extracellular DNA....Pages 85-89 A Historical and Evolutionary Perspective on Circulating Nucleic Acids and Extracellular Vesicles: Circulating Nucleic Acids as Homeostatic Genetic Entities....Pages 91-95 Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs....Pages 97-100 A Quantitative Assessment of Cell-Free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different Cell Lines....Pages 101-103 Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-Induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-Dependent Manner....Pages 105-107 Front Matter....Pages 77-77 GC-Rich DNA Fragments and Oxidized Cell-Free DNA Have Different Effects on NF-kB and NRF2 Signaling in MSC....Pages 109-112 Evaluation of the State of Transplanted Liver Health by Monitoring of Organ-Specific Genomic Marker in Circulating DNA from Receptor....Pages 113-116 Vesicular and Extra-Vesicular RNAs of Human Blood Plasma....Pages 117-119 Artificial Analogues of Circulating Box C/D RNAs Induce Strong Innate Immune Response and MicroRNA Activation in Human Adenocarcinoma Cells....Pages 121-125 Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells....Pages 127-131 Protein Content of Circulating Nucleoprotein Complexes....Pages 133-136 Front Matter....Pages 137-137 Digital PCR of Genomic Rearrangements for Monitoring Circulating Tumour DNA....Pages 139-146 mFast-SeqS as a Monitoring and Pre-screening Tool for Tumor-Specific Aneuploidy in Plasma DNA....Pages 147-155 Methodological Variables in the Analysis of Cell-Free DNA....Pages 157-163 Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing....Pages 165-169 A Rapid and Sensitive Method for Detection of the T790M Mutation of EGFR in Plasma DNA....Pages 171-174 Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker....Pages 175-178 Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument....Pages 179-185 Detection and Quantification of KIT Mutations in ctDNA by Plasma Safe-SeqS....Pages 187-189 Front Matter....Pages 191-191 Lost in Translation? Ethical Challenges of Implementing a New Diagnostic Procedure....Pages 193-197 Front Matter....Pages 199-199 Academia Meets Industry....Pages 201-215 Back Matter....Pages 217-220