Circulating Nucleic Acids in Plasma and Serum : Proceedings of the 6th International Conference on Circulating Nucleic Acids in Plasma and Serum Held on 9-11 November 2009 in Hong Kong.

DNA and RNA fractions have been isolated from a variety of sources including: whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and patients. The ability to isolate, quantify, and analyze these molecules has led to the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis. This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong. The topics that are covered in these proceedings include: - Nucleic Acids in Oncology - Nucleic Acids in Foetal Medicine - The Biology of CNAPS - New Technologies for CNAPS - Other Clinical Exploitation of CNAPS Front Matter....Pages i-xx Front Matter....Pages 1-1 Current Developments in Circulating Nucleic Acids in Plasma and Serum....Pages 3-12 Front Matter....Pages 13-13 Reflections on a Life of CNAPS: From Circulating DNA to the Virtosome....Pages 15-20 Circulating Tumor-Related DNA Alterations as Prostate Cancer Biomarkers....Pages 21-27 Parallel Tagged 454 Sequencing for the Characterization of Circulating DNA....Pages 29-33 Advanced Analysis of Human Plasma Circulating DNA Sequences Produced by Parallel Tagged Sequencing on the 454 Platform....Pages 35-40 Concentration and Distribution of Single-Copy β -Actin Gene and LINE-1 Repetitive Elements in Blood of Lung Cancer Patients....Pages 41-45 Plasma DNA Methylation Analysis in Predicting Short-Term Recurrence of Surgical Patients with Non-small Cell Lung Cancer (NSCLC)....Pages 47-55 Blood Based Methylated DNA and Tumor-Specific Protein Analysis in Gastric Cancer Diagnostics....Pages 57-61 Increase in Circulating MicroRNA Levels in Blood of Ovarian Cancer Patients....Pages 63-71 The Course of Circulating Nucleosomes in Liver Cancer Patients Undergoing Transarterial Chemoembolization Therapy....Pages 73-77 Presence of Nucleosomes in Cerebrospinal Fluid of Glioblastoma Patients – Potential for Therapy Monitoring....Pages 79-84 Circulating Nucleosomes and DNAse in Breast Cancer Patients During Neoadjuvant Chemotherapy....Pages 85-89 Circulating Nucleosomes in Cancer Patients with Liver Metastases Undergoing Selective Internal Radiation Therapy Using Yttrium-90 Labelled Microspheres....Pages 91-95 H3K9me3/H4K20me3 Ratio in Circulating Nucleosomes as Potential Biomarker for Colorectal Cancer....Pages 97-103 Functionality of CNAPS in Cancer: The Theory of Genometastasis....Pages 105-108 Front Matter....Pages 109-109 Circulating Fetal DNA/RNA in Maternal Plasma for Aneuploidy Detection....Pages 111-124 A “Fluid-Agnostic” Approach to Analysis of Fetal and Neonatal Developmental Gene Expression....Pages 125-132 Non-invasive Prenatal Diagnosis: An Epigenetic Approach to the Detection of Common Fetal Chromosome Disorders by Analysis of Maternal Blood Samples....Pages 133-142 Comparative Study of Extracellular DNA by FISH....Pages 143-146 An Additional Pre-amplification Step for the Early Determination of Fetal RHD from Maternal Plasma....Pages 147-151 Front Matter....Pages 109-109 The Correlation of Circulating Cell-Free DNA, Cell-Free Fetal DNA and MicroRNA 325 Levels to Clinical Characteristics and Laboratory Parameters in Pre-eclampsia....Pages 153-156 Front Matter....Pages 157-157 Comparison of Plasma Cell-Free DNA Levels with Gene Expression Profiles of Peripheral Blood Cells During Haemodialysis....Pages 159-163 Low-Molecular-Weight DNA of Blood Plasma as an Indicator of Pathological Processes....Pages 165-170 The Clinical Significance of Plasma DNA Quantification for Quake Trauma Patients....Pages 171-182 Front Matter....Pages 183-183 Methylated Cell-Free DNA In Vitro and In Vivo....Pages 185-194 Circadian Rhythmicity and Clearance of Cell-Free DNA in Human Plasma....Pages 195-198 Fragments of Cell-Free DNA (cfDNA) Enhance Transcription Activity in Human Mesenchymal Stem Cells (hMSCs) and Inhibit Their In Vitro Differentiation....Pages 199-205 Cell-Surface-Bound DNA Inhibits Poly(I:C)-Activated IL-6 and IL-8 Production in Human Primary Endothelial Cells and Fibroblasts....Pages 207-211 Accumulating Fragments of Extracellular DNA (ecDNA) Influence Rat Primary Cerebellum Granule Cell Culture....Pages 213-218 Cell Free DNA (cfDNA) Influences Nitric Oxide and ros Levels in Human Endothelial Cells....Pages 219-223 Development of the Adaptive Response and Bystander Effect Induced by Low-Dose Ionising Radiation in Human Mesenchymal Stem Cells....Pages 225-231 Extracellular RNA as Regulators of Cellular Processes....Pages 233-237 Microvesicles Circulating in Plasma of Rats Contain DNA: Are These Small Vesicles a Main Source of Cell-Free DNA in Plasma?....Pages 239-244 Front Matter....Pages 245-245 Rapid Isolation and Detection of Cell Free Circulating DNA and Other Disease Biomarkers Directly from Whole Blood....Pages 247-257 Yields of Viral and Circulating Cell-Free Nucleic Acids Using the QIAamp ® Circulating Nucleic Acid Kit....Pages 259-268 Comparison of Nucleosomes and Quantitative PCR Using Diverse DNA Isolation Methods....Pages 269-273 MicroRNA Analysis in the Spinal Fluid of Alzheimer Patients: A Methodological Feasibility Study....Pages 275-282 Back Matter....Pages 283-285 DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.