Cholesterol Modulation of Protein Function: Sterol Specificity and Indirect Mechanisms (Advances in Experimental Medicine and Biology, 1115)

"In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems"--Publisher's description Front Matter ....Pages i-x Front Matter ....Pages 1-1 Chirality Effect on Cholesterol Modulation of Protein Function (Jitendra D. Belani)....Pages 3-19 A Critical Analysis of Molecular Mechanisms Underlying Membrane Cholesterol Sensitivity of GPCRs (Md. Jafurulla, G. Aditya Kumar, Bhagyashree D. Rao, Amitabha Chattopadhyay)....Pages 21-52 Regulation of BK Channel Activity by Cholesterol and Its Derivatives (Anna N. Bukiya, Alex M. Dopico)....Pages 53-75 Chiral Specificity of Cholesterol Orientation Within Cholesterol Binding Sites in Inwardly Rectifying K+ Channels (Nicolas Barbera, Irena Levitan)....Pages 77-95 Front Matter ....Pages 97-97 Cholesterol Effects on the Physical Properties of Lipid Membranes Viewed by Solid-state NMR Spectroscopy (Trivikram R. Molugu, Michael F. Brown)....Pages 99-133 Effect of Cholesterol on the Dipole Potential of Lipid Membranes (Ronald J. Clarke)....Pages 135-154 Mass Spectrometry Imaging of Cholesterol (Stephanie M. Cologna)....Pages 155-166 Cholesterol-Dependent Gating Effects on Ion Channels (Qiu-Xing Jiang)....Pages 167-190 Back Matter ....Pages 191-197