Chirurgisches Forum und DGAV Forum 2009: für experimentelle und klinische Forschung 126. Kongress der Deutschen Gesellschaft für Chirurgie München, 28.04.-01.05.2009

Über 100 Beiträge ausgewählt vom Forum-Ausschuss der Deutschen Gesellschaft für Chirurgie für das Chirurgische Forum 2009 und von Gutachtern der Deutschen Gesellschaft für Allgemein- und Viszeralchirurgie für das DGAV Forum 2009, geben den Stand der experimentellen und klinischen Forschung wieder. Sie finden die Vorträge der Fritz-Linder-Preisträgersitzung und weitere Arbeiten aus vielen Bereichen der Chirurgie. Onkologie, Molekulare Biologie, Stammzellenforschung, Pathophysiologie, Transplantation, Tissue Engineering, Biomaterialien, Klinische Studien Sichern Sie sich einen exzellenten Überblick über die aktuellen Forschungsschwerpunkte und Forschungsergebnisse in der Chirurgie und die Perspektiven, die sich für Diagnostik und Therapie ergeben. Front Matter....Pages I-XXIV MicroRNA miR-155 is a biomarker of early pancreatic neoplasia....Pages 1-2 Targeted stem cell based RANTES/TK suicide gene-therapy in a murine pancreatic cancer tumour model....Pages 3-5 Chronic inflammation and tumor progression: Analysis of Toll-like receptor expression in colorectal carcinoma....Pages 7-8 Role of four and one half LIM domain protein FHL2 on intestinal anastomotic healing....Pages 9-10 Studies on biocompatibility and interaction of silver nanoparticles on human mesenchymal stem cells (hMSCs)....Pages 11-13 Prednisolon reduces the ATG-dependent microvascular thrombus formation in postischemic tissue in vivo....Pages 15-16 Overexpression of the gene IFIT3 enhances tumor growth, angiogenesis, metastasing and chemoresistance of the pancreas carcinoma cells....Pages 17-18 Enhanced CXC-chemokine expression in patients with malignant pancreatic diseases....Pages 19-20 Expression and regulation of Krüppel-like factor 5 (KLF5) in human pancreatic cancer cells: a new molecular target?....Pages 21-22 Host Defense peptides are upregulated in tumors of the upper pharynx....Pages 23-25 Identification and functional validation of potential target genes in colorectal cancers....Pages 27-28 Identification of a MSI-H tumor specific cytotoxic T cell epitope generated by the (−1) frame of U79260(FTO)....Pages 29-30 The role of the transcription factor ZEB1 in epithelial-mesenchymal transition and progression of pancreatic carcinoma....Pages 31-32 Loss of 15-Lipoxygenase expression during pancreatic carcinogenesis provides growth advantage for tumor cells....Pages 33-35 Culture and malignant transformation of pancreatic β-cells in the hamster model....Pages 37-38 Epithelial-mesenchymal transition in parathyroid neoplasms....Pages 39-40 Characterization of the role of CK1δ in tumorogenesis and tumor progression of the mamma carcinoma using a SV40 T-Ag/mtCK1δ bitransgenic mouse model....Pages 41-42 CCR6 involvement in tumor cell homing to the liver....Pages 43-44 Identification of pancreatic cancer initiating cells: The role of the MDR gene ABCB5....Pages 45-47 Simultaneous gene-inhibtion of Survivin, XIAP and Bcl-2 re-sensitises pancreatic cancer cell line MIA PaCa-2 to apoptosis....Pages 49-50 Inhibition of the enzyme Dihydroorotate Dehydrogenase by Leflunomid and the active metabolite A 771726 inhibits in vitro tumour cell proliferation and in vivo tumour growth in pancreatic carcinoma SCID mouse model....Pages 51-53 Tumor stem cell targeted therapy with mTOR inhibitor RAD001 and hedgehog signalling inhibitor Cyclopamine reverts chemoresistance towards 5-Fluorouracil in human pancreatic carcinoma cells....Pages 55-56 Gemcitabine therapy in pancreas carcinoma: Dependence of survival on gene polymorphisms in the nucleoside transporters ENT1 and CNT1....Pages 57-59 Bacteriolytic therapy of experimental pancreatic carcinoma....Pages 61-62 Molecular characterization of disseminated tumor cells from colorectal cancer: nuclear beta-catenin defines subpopulation with higher chromosomal instability (CIN)....Pages 63-64 Selection of efficient drugs for the therapy of gastrointestinal cancers using the multicellular spheroid model....Pages 65-66 Inflammation-associated inhibition of angiogenesis in colorectal carcinoma: molecular mechanisms and prognostic impact....Pages 67-68 Rapamycin inhibits hepatectomy-induced stimulation of metastatic tumor growth by reduction of angiogenesis and tumor cell proliferation....Pages 69-70 Darbepoetin promotes tumor growth and angiogenesis of colorectal liver metastases particularly after liver resection....Pages 71-72 Hepatic-arterial infusion of bevacizumab, particularly in combination with oxaliplatin, reduces growth of CC531 colorectal metastasis confined to the liver....Pages 73-74 Biocompatibility and pharmacokinetics of in erythrocytes encapsulated chemotherapeutics for local chemotherapy....Pages 75-76 Laparoscopic sentinel lymph node mapping and excision of the rectum using a radioactive tracer — a prospective experimental study....Pages 77-79 First Resection of a Non Visible, Non Palpable Liver tumour Using Modern Visualization Techniques and Navigation....Pages 81-83 Electro-physiological parameters of hepatic radiofrequency ablation — a comparison of in vitro vs. in vivo porcine liver model....Pages 85-87 Uncontrolled cooling effects of intrahepatic vessels by RF ablation: An ex vivo study about the influence of vessel diameter and flow velocity on heat dissipation during therapy....Pages 89-91 Ex vivo quantification of the cooling effect of liver vessels on radiofrequency ablation volume....Pages 93-94 Prediction of prognosis by expression profiling analysis of pancreatic ductal adenocancinoma using DNA-arrays....Pages 95-97 XPC 939 and XRCC3 polymorphisms as prognostic markers in locally advanced esophageal and gastric adenocarcinoma treated with cisplatin and 5-FU based neoadjuvant chemotherapy....Pages 99-101 Impact of ERCC1 gene polymorphisms for prognosis in gastric cancer....Pages 103-104 Correlation of molecular profiles and clinical course of stage II colon cancer patients....Pages 105-106 The Hedgehog inhibitor cyclopamine is an effective chemopreventive and chemotherapeutic agent in transgenic Rip1Tag2 mice....Pages 107-108 A phase I/II clinical trial in patients with locally advanced and/or metastatic pancreatic cancer using a combination of low dose chemotherapy and RAD 001....Pages 109-110 PDGFRA-overexpression is associated with a favourable prognosis after resection of primary gastrointestinal stromal tumors — an analysis of mutation and expression status of KIT and PDGFR-alpha in 109 resected patients....Pages 111-113 Prognostic significance of EpCAM positive tumour cells in perirectal lymph nodes from patients with rectal carcinoma UICC stage I....Pages 115-116 Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer....Pages 117-118 p16 Expression Differentiates High-Risk Gastrointestinal Stromal Tumors and Predicts Poor Outcome....Pages 119-122 Neoexpression of EpCAM in Esophageal Squamous Cell Carcinoma — Prognostic Marker and Potential Therapeutic Target....Pages 123-124 Down-regulation of EpCAM correlates with an unfavorable Prognosis in the Metaplasia-Carcinoma-Sequence of Barrett’s Carcinoma....Pages 125-126 Impact of the IDO2 blocker D-1-methyltryptophan on the IDO activity of human tumors and dendritic cells....Pages 127-129 Does expression of receptor tyrosine kinases in gastric adenocarcinoma correlate with clinicopathological parameters?....Pages 131-133 The value of survivin gene expression in peripheral blood as a non-invasive prognostic factor in patients with esophageal cancer....Pages 135-136 Erythropoietin reduces ischemia-reperfusion injury after fatty liver transplantation in rats....Pages 137-139 Impairment of insulin production is induced by a toxic tacrolimus dosage....Pages 141-143 Pancreatic-duodenal homeobox 1 (Pdx1) expression distinguishes between duodenal and pancreatic gastrinomas....Pages 145-146 Mutations of the RET proto-oncogene in medullary thyroid cancer....Pages 147-149 NeoHepatocytes generated from patients with chronic liver disease can be used for autologous cell transplantation....Pages 151-153 The application of autologous serum improves production of NeoHepatocytes for cell transplantation....Pages 155-156 Pharmacological inhibition of cytokine synthesis with CPSI-2364 ameliorates ischemia reperfusion injury (IRI) after small bowel transplantation (SBTx)....Pages 157-158 A causal role of C-reactive protein (CRP) in inflammation: Conformational rearrangement in C-reactive protein is required for enhanced pro-inflammatory leukocyte activation and leukocyte adhesion in ischemiareperfusion of the striated muscle....Pages 159-160 Cell-based therapy using endothelial progenitor cells rescues kidney from ischemia-reperfusion injury....Pages 161-162 The role of Endothelial Cell-Selective Adhesion Molecule (ESAM) for leukocyte migration and vascular permeability during hepatic ischemia-reperfusion....Pages 163-164 Transient portal ischaemia and irradiation as preparative regimen for liver repopulation....Pages 165-166 Intravital microscopic analysis of vascularization of transplanted pancreatic islets, pseudoislets and modified pseudoislets....Pages 167-169 The dual role of tetrahydrobiopterin in the prevention of early parenchymal damage following murine pancreas transplantation....Pages 171-173 Do blood monocytes form gap-junctions in renal grafts?....Pages 175-176 IL-15 determines the role of Natural Killer cells in alloimmune response....Pages 177-178 Intrahepatic Memory-T-cells dominate the immune response of naive recipients following mouse liver transplantation....Pages 179-180 Donor treatment with Simvastatin reduces graft immunogenicity following prolonged cold ischemia and ameliorates long-term survival....Pages 181-183 Age-dependent immune response after organ transplantation....Pages 185-186 Inhalation of prostacyclin reduces edema formation in flushed and ventilated isolated pig lungs....Pages 187-189 Partial Hepatectomy is paralleled by peripheral, resection-volume depending mobilisation of CD133+ stem cells....Pages 191-193 Negative hepatic arterial buffer response (HABR) after major partial hepatectomy is counter-balanced by nitric oxide liberation....Pages 195-196 Small hepatocytes (SH) for an alternative liver therapy....Pages 197-198 The hepatotrophic growth factor Augmenter of liver regeneration (ALR) is protective against metabolic damage via the PI3K/AKT pathway....Pages 199-200 Angiotensin-1-receptor blocking improves regeneration and reduces fibrosis after resection of cirrhotic rat livers....Pages 201-202 Does the highly selective cyclooxigenase-2-inhibitor Parecoxib decrease eicosanoid metabolism and histopathological severity of acute necrotizing pancreatitis in rats?....Pages 203-204 Impact of low-molecular-weight heparin on tumour growth and apoptosis in ductal pancreatic cancer....Pages 205-207 Differential expression of COL11A1 in chronic pancreatitis and periampullary adenocarcinomas....Pages 209-210 Does the high-selective Cyclooxygenase-2-Inhibitior Parecoxib induce apoptosis and necrosis in pancreatic cancer?....Pages 211-213 Inhibition of Sonic Hedgehog signaling reduces tumor growth in pancreatic cancer in vitro and in vivo....Pages 215-216 Mechanical stretch aggravates the LPS-induced inflammatory reaction in intestinal smooth muscle cells and macrophages....Pages 217-219 An enteral immunonutrition with long chain triglycerides significantly reduces the recruitment of immune cells into the liver and apotosis during experimental sepsis....Pages 221-222 Characterisation of tight junction and adherens junction in an inflammatory model of cultured human peritoneal mesothelial cells....Pages 223-224 Annexin A1 regulates intestinal mucosal injury, inflammation, and repair....Pages 225-227 Preoperative application of CPSI 2364 per os prevents postoperative ileus in rodents and swine....Pages 229-231 Enhanced activity of immunoproteasomes in patiens with Crohn’s disease....Pages 233-234 Detection and quantification of defensin positive cells in the ileac submucosal layer of patients with Crohn’s disease under medical treatment....Pages 235-237 Percutaneous-endoscopic Sacral Nerve Stimulation in a sheep model — Feasibility of an Endoscopic procedure for Sacral Nerve Stimulation in the Treatment of Fecal Incontinence....Pages 239-240 Comparative proteomics-analysis for the isolation of markers for metastasis of the colorectal carcinoma....Pages 241-243 Epithelial-Mesenchymal-Transition (EMT): The role of E-cadherin transcription regulators SIP1, Twist and Snail in colorectal adenomas....Pages 245-246 Comparative analysis of endothelial cells and fibroblasts from human colorectal cacinoma....Pages 247-248 Kinetics of inflammatory mediators in peritoneal adhesions....Pages 249-252 Chronic alcohol ingestion increases hepatic and pulmonary damage in experimental necrotizing pancreatitis....Pages 253-255 Molecular analysis of early disseminated cells from esophageal cancer patients: EpCAM, a potential target for adjuvant therapy, is rarely expressed on cytokeratin-positive disseminated tumor cells....Pages 257-258 Establishment of a standardized septic rat model....Pages 259-260 The impact of cGMP and cAMP dependent pathways on the differentiation of human preadipocytes....Pages 261-262 Development and validation of a new bioreactor for cyclic loading of bovine articular osteochondral explants over a period of 14 days....Pages 263-264 Biodegradable shape-memory polymers as implant materials....Pages 265-268 In vivo analysis of biocompatibility and vascularization of elastic polyurethane scaffolds for tissue engineering....Pages 269-270 Evaluation of Small Intestinal Submucosa (SIS) for Colonic Tissue Regeneration in a large, animal model....Pages 271-272 Bioartificial vascularized human tissues as implants for reconstructive surgery: Implant generation and first clinical results....Pages 273-274 Magnetofection of acoustically active „magnetic microspheres“ (Magnetobubbles) — Induction of angiogenesis via non-viral transfection of VEGF....Pages 275-276 Pharmacological induction of the “delay phenomenon”: Ghrelin, a gastric peptide to prevent musculocutaneous necrosis from ischemia?....Pages 277-279 Simvastatin reduces ischemia-induced inflammation and stimulates neovascularisation of critically perfused flaps....Pages 281-283 Embryonic Stem Cell Derived Motor Neurons Form Neuromuscular Junctions in vitro and Enhance Motor Functional Recovery in vivo....Pages 285-286 Improved Regeneration of Autologous Nerve Transplants by Means of VEGF-Gene Therapy....Pages 287-288 Peripheral nervous system: Neuro-Tissue Engineering using a microstructured collagen matrix....Pages 289-291 Signal transduction of the innate and adaptive immune system after transient cutaneous adenoviral gene delivery....Pages 293-295 Beyond epithelial to mesenchymal transition: A novel role for the transcription factor Snail in inflammation and wound healing....Pages 297-298 Lactate induces collagen synthesis, VEGF expression and migration in endothelial cells via generation of superoxide....Pages 299-301 Application of FGF-2 mimics bone healing potential of frontal bones in parietal calvarial defects....Pages 303-304 Effects of phytoestrogens and estrogen on fracture healing in severe experimental osteoporotic bone: Equol and estrogen improve — Genistein inhibits....Pages 305-307 Investigation of the biocompatibility of cell-loaded nickel titanium under dynamic mechanical loading....Pages 309-310 Immune response to xenogeneic extracellular matrix grafts: „Remodeling rather than rejection“....Pages 311-312 Cellular expression of cathepsin proteases in symptomatic and asymptomatic AAA....Pages 313-315 Nitric oxide as a stimulator for the expression of TRPV-4 in collateral vessels during arteriogenesis....Pages 317-320 Quantitative MRI-based measurement of blood flow in the rat-circle of Willis by Bruker PharmaScan 70/16....Pages 321-323 Could cell therapy for the treatment of microangiopathic and diabetic lesions be a promising alternative to limb amputation?....Pages 325-327 Improvement of translational and clinical research introducing quality management....Pages 329-330 Laparoscopic fluorescence angiography with indocyanine green to control the perfusion of colorectal anastomoses intraoperatively....Pages 331-333 The use of a silicone spray for prevention and treatment of scars: a double blinded study of 20 patients....Pages 335-337 Back Matter....Pages 339-345 Über 100 sorgfältig ausgewählte Arbeiten zeigen das breite Spektrum der deutschsprachigen chirurgischen Forschung. Behandelt werden Themen aus allen Bereichen der Chirurgie – unter Berücksichtigung der klinischen Forschung wie der Grundlagenforschung. Der Band zum neuesten Stand der chirurgischen Forschung erscheint noch vor der Präsentation der Ergebnisse bei der 126. Jahrestagung der Deutschen Gesellschaft für Chirurgie.