Chemistry of Metalloproteins: Problems and Solutions in Bioinorganic Chemistry (Wiley Series in Protein and Peptide Science)

**Addresses the full gamut of questions in metalloprotein science** Formatted as a question-and-answer guide, this book examines all major families of metal binding proteins, presenting our most current understanding of their structural, physicochemical, and functional properties. Moreover, it introduces new and emerging medical applications of metalloproteins. Readers will discover both the underlying chemistry and biology of this important area of research in bioinorganic chemistry. __Chemistry of Metalloproteins__ features a building block approach that enables readers to master the basics and then advance to more sophisticated topics. The book begins with a general introduction to bioinorganic chemistry and metalloproteins. Next, it covers: * Alkali and alkaline earth cations * Metalloenzymes * Copper proteins * Iron proteins * Vitamin B~12~ * Chlorophyll Chapters are richly illustrated to help readers fully grasp all the chemical concepts that govern the biological action of metalloproteins. In addition, each chapter ends with a list of suggested original research articles and reviews for further investigation of individual topics. Presenting our most current understanding of metalloproteins, __Chemistry of Metalloproteins__ is recommended for students and researchers in coordination chemistry, biology, and medicine. Each volume of the Wiley Series in Protein and Peptide Science addresses a specific facet of the field, reviewing the latest findings and presenting a broad range of perspectives. The volumes in this series constitute essential reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, and physiologists as well as researchers in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.

Addresses the full gamut of questions in metalloprotein science

Formatted as a question-and-answer guide, this book examines all major families of metal binding proteins, presenting our most current understanding of their structural, physicochemical, and functional properties. Moreover, it introduces new and emerging medical applications of metalloproteins. Readers will discover both the underlying chemistry and biology of this important area of research in bioinorganic chemistry.

Chemistry of Metalloproteins features a building block approach that enables readers to master the basics and then advance to more sophisticated topics. The book begins with a general introduction to bioinorganic chemistry and metalloproteins. Next, it covers:

• Alkali and alkaline earth cations
• Metalloenzymes
• Copper proteins
• Iron proteins
• Vitamin B 12
• Chlorophyll

Chapters are richly illustrated to help readers fully grasp all the chemical concepts that govern the biological action of metalloproteins. In addition, each chapter ends with a list of suggested original research articles and reviews for further investigation of individual topics.

Presenting our most current understanding of metalloproteins, Chemistry of Metalloproteins is recommended for students and researchers in coordination chemistry, biology, and medicine.

Each volume of the Wiley Series in Protein and Peptide Science addresses a specific facet of the field, reviewing the latest findings and presenting a broad range of perspectives. The volumes in this series constitute essential reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, and physiologists as well as researchers in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides.

Content: Preface ix 1 Introduction 1 Proteins: Formation, Structures, and Metalloproteins, 4 References, 28 2 Alkali and Alkaline Earth Cations 31 References, 67 3 Nonredox Metalloenzymes 71 Carboxypeptidases, 75 Carbonic Anhydrase, 84 Alcohol Dehydrogenase, 88 References, 91 4 Copper Proteins 95 Introduction, 95 Electronic Spectra of Copper Ions, 96 ESR Spectra of Copper Ions, 105 Copper Proteins, 117 Plastocyanin, 119 Azurin and Stellacyanin, 127 Superoxide Dismutase, 131 Hemocyanin, 135 Ascorbic Oxidase, 139 References, 142 5 Iron Proteins 147 Introduction, 147 Electronic Spectra of Iron Ions, 148 Mossbauer Spectroscopy of Iron Ions, 155 ESR Spectra of Iron (III), 161 Iron Bioavailability, 166 Siderophores, 171 Iron Storage and Transfer Proteins, 184 Ferritin, 184 Transferrin, 187 Dioxygenase Iron Proteins, 195 Iron-Sulfur Proteins, 207 Rubredoxin, 207 Ferredoxins, 212 2Fe-2S Ferredoxins, 212 4Fe-4S Ferredoxins, 221 Aconitase, 226 Hydroxylases, 229 Hydrogenases, 232 Nitrogenases, 240 Binuclear Iron Proteins, 251 Hemerythrin, 253 Ribotide Reductase, Purple Acid Phosphate, and Methane Monooxygenase, 260 Hemoproteins: Classification and Behavior of Heme in Absence of Globins, 267 Myoglobin and Hemoglobin, 275 Myoglobin, 275 Hemoglobin, 280 Cytochrome C, 298 Electron Transfer in Porphyrins and Metalloporphyrins, 301 Catalases, 311 Peroxidases, 315 Cytochrome P-450, 322 Electronic Spectra of Hemoproteins, 327 ESR Spectra of Hemoproteins, 362 References, 378 6 Vitamin B12 393 References, 405 7 Chlorophyll 407 References, 421 Index 423 Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies—and on their use in biotechnological and medical applications The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications. Protein Aggregation in Bacteria covers: Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria Physiological importance and consequences of aggregation for the bacterial cell Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science. "This question-and-answer guide on metalloproteins reflects the broad range of aspects within this branch of protein science. It is divided into seven parts, addressing: the cell, the alkali and alkaline earth cations, non-redox metalloenzymes, carboxypeptases, carbonic anhydrase, alcohol dehydrogenase, copper proteins, electronic and ESR spectra of copper ions, plastocyanin, stellacyanin, superoxide dismutase, electronic spectra of iron ions, Mossbauer spectroscopy of iron ions, ESR spectra of Iion (III), iron bioavailability, siderophores, non-heme iron proteins, ferritin, transferrin, dioxygenase-iron proteins, rubredoxin, 2Fe-2S Ferredoxins, 4FE-4S Ferredoxins and hiPIP, aconitase, hydroxylases, hydrogenases, nitrogenases, binuclear Fe proteins, hemerythrin, ribotide reductase, purple acid phosphate and methane mono-oxygenase, heme proteins, myoglobin and hemoglobin, electronic spectra of hemoproteins, cytochrome C, peroxidase, cytochrome P450, ESR spectra of hemoproteins, vitamin B12, and chlorophyll. Each section ends with suggestions for further reading"--Provided by publisher. � Read more... Abstract: Addresses the full gamut of questions in metalloprotein science. This book examines all major families of metal binding proteins, presenting our understanding of their structural, physicochemical, and functional properties. It introduces medical applications of metalloproteins. � Read more... "This question-and-answer guide on metalloproteins reflects the broad range of aspects within this branch of protein science. It is divided into seven parts, addressing: the cell, the alkali and alkaline earth cations, non-redox metalloenzymes, carboxypeptases, carbonic anhydrase, alcohol dehydrogenase, copper proteins, electronic and ESR spectra of copper ions, plastocyanin, stellacyanin, superoxide dismutase, electronic spectra of iron ions, Mossbauer spectroscopy of iron ions, ESR spectra of Iion (III), iron bioavailability, siderophores, non-heme iron proteins, ferritin, transferrin, dioxygenase-iron proteins, rubredoxin, 2Fe-2S Ferredoxins, 4FE-4S Ferredoxins and hiPIP, aconitase, hydroxylases, hydrogenases, nitrogenases, binuclear Fe proteins, hemerythrin, ribotide reductase, purple acid phosphate and methane mono-oxygenase, heme proteins, myoglobin and hemoglobin, electronic spectra of hemoproteins, cytochrome C, peroxidase, cytochrome P450, ESR spectra of hemoproteins, vitamin B12, and chlorophyll. Each section ends with suggestions for further reading"-- Provided by publisher This book focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies. Recent reports revolutionized the current view of inclusion bodies from that of inert deposits of inactive proteins to reservoirs of proteins that can eventually maintain biological activity and/or be rescued by cells. Aggregation is put in the context of updated knowledge about the folding and aggregation of proteins in simple cells and new perspectives derived from the application of this knowledge are presented. The following topics are addressed: a) molecular and c