Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications (RNA Technologies)

La 4e de couv. indique : "This volume contains 29 engrossing chapters contributed by worldwide, leading research groups in the field of chemical biology. Topics include pre-biology; the establishment of the genetic code; isomerization of RNA; damage of nucleobases in RNA; the dynamic structure of nucleic acids and their analogs in DNA replication, extra- and intra-cellular transport; molecular crowding by the use of ionic liquids; new technologies enabling the modification of gene expression via editing of therapeutic genes; the use of riboswitches; the modification of mRNA cap regions; new approaches to detect appropriately modified RNAs with EPR spectroscopy and the use of parallel and high-throughput techniques for the analysis of the structure and new functions of nucleic acids. This volume discusses how chemistry can add new frontiers to the field of nucleic acids in molecular medicine, biotechnology and nanotechnology and is not only an invaluable source of information to chemists, biochemists and life scientists but will also stimulate future research." Front Matter....Pages i-xi RNA as Major Components in Chemical Evolvable Systems....Pages 1-24 How the Early Genetic Code Was Established?: Inference from the Analysis of Extant Animal Mitochondrial Decoding Systems....Pages 25-40 Isomerization of RNA Phosphodiester Linkages....Pages 41-56 Effects of Ionic Liquid and Liposomes on the Structure, Stability, and Function of Nucleic Acids....Pages 57-74 Oxidative Damage on RNA Nucleobases....Pages 75-94 Use of FRET to Study Dynamics of DNA Replication....Pages 95-111 Design, Characterization, and Application of Imidazopyridopyrimidine:Naphthyridine Base-Pairing Motifs Consisting of Four Hydrogen Bonds....Pages 113-129 Creation of Unnatural Base Pair Systems Toward New DNA/RNA Biotechnologies....Pages 131-148 Flexible Nucleobase Analogues: Novel Tools for Exploring Nucleic Acids....Pages 149-165 Sequence-Selective Recognition of Double-Stranded RNA....Pages 167-180 Determining Transient Nucleic Acid Structures by NMR....Pages 181-198 Diastereomer-Specific Repertoire of 7′ R - or 7′ S -Me-Carba- L ocked N ucleic A cids (cLNAs) in Antisense Oligo/RNA Duplexes and Engineering of Physico-chemical and Enzymological Properties....Pages 199-226 Challenges and Opportunities for Oligonucleotide-Based Therapeutics by Antisense and RNA Interference Mechanisms....Pages 227-242 Progress in Chemically Modified Nucleic Acid Aptamers....Pages 243-270 Aptamers as Molecular Smugglers....Pages 271-292 Biochemical Aspects of Subcellular RNA Transport and Localization....Pages 293-308 Small Size, Big Impact: Bacterial Functional Nucleic Acids and Their Applications....Pages 309-323 Towards Defined DNA and RNA Delivery Vehicles Using Nucleic Acid Nanotechnology....Pages 325-345 Targeted Editing of Therapeutic Genes Using DNA-Based Transcriptional Activators: Scope and Challenges....Pages 347-365 Interaction of DNA Intramolecular Structures with Their Complementary Strands: A Thermodynamic Approach for the Control of Gene Expression....Pages 367-383 Site-Directed Spin Labeling of RNA for Distance Measurements by EPR....Pages 385-407 Chemo-enzymatic Strategies to Modify RNA in vitro or in Living Cells....Pages 409-421 Metal Dependence of Ligand Binding and Heavy-Atom Derivatization of Evolutionarily Distinct PreQ 1 Riboswitches....Pages 423-440 DNA G-Quadruplexes and I-Motifs in Therapeutics and Diagnostics....Pages 441-458 Peptides Targeting G-Quadruplex Structures....Pages 459-475 Synthesis of Site-Specifically Modified Long-mer RNAs....Pages 477-496 Synthesis and Exon-Skipping Activity of Chemically Modified RNAs....Pages 497-510 mRNA and snRNA Cap Analogs: Synthesis and Applications....Pages 511-561 Innovative Chemistry for Synthesis of Regular RNA, 5′-Triphosphate RNA, or 5′-Capped RNA....Pages 563-589 Back Matter....Pages 591-599