Cellular Fatty Acid-Binding Proteins II : Proceedings of the 2nd International Workshop on Fatty Acid-Binding Proteins, Maastricht, August 31 and September 1, 1992

Twenty years have elapsed since cytoplasmic proteins exhibiting high-affinity binding of long-chain fatty acids were first identified (Ockner et al., Science 177:56-58, 1972). These cellular fatty acid-binding proteins (FABPs) are now well established to comprise a ligand-defined group of macromolecules belonging to a family of cytoplasmic lipid binding proteins. Unique features of the FABPs are the existence of distinct types of FABP and that these are found in a variety of tissues in remarkable abundance, with some cells expressing more than one type. The physiological significance of the FABPs has only partly been elucidated. By increasing the cytoplasmic solubilization of fatty acids, the cellular FABPs are considered to function primarily in intracellular fatty acid transport, but may also be assigned important regulatory roles in cellular lipid homeostasis as well as in the modulation of cell growth and differentiation. The broad interests in cellular FABPs has led to the organization of the 1st International Workshop on Fatty Acid-Binding Protein, held in Maastricht, the Netherlands, in 1989. Prompted by the success of the first meeting, the 2nd International Workshop on Fatty-Acid-Binding Proteins, which was held again in Maastricht, on August 31 and September 1, 1992, brought together scientific scpecialists in the field of FABP research for two days of intensive and fruitful discussion. This volume is a collection of selected papers from this conference, and thus provides the state-of-the-art knowledge of cellular FABPs. The contributors to this issue represent pioneering as well as new investigators, and also reflect the multidisciplinary nature of research in this exciting and rapidly progressing field. Front Matter....Pages i-vi Preface....Pages 1-1 High resolution X-ray studies of mammalian intestinal and muscle fatty acid-binding proteins provide an opportunity for defining the chemical nature of fatty acid: protein interactions....Pages 3-13 Solution structure of bovine heart fatty acid-binding protein (H-FABP C )....Pages 15-22 Involvement of arginine in the binding of heme and fatty acids to fatty acid-binding protein from bovine liver....Pages 23-27 Titration calorimetry as a binding assay for lipid-binding proteins....Pages 29-37 Identification of high affinity membrane-bound fatty acid-binding proteins using a photoreactive fatty acid....Pages 39-44 Diversity of fatty acid-binding protein structure and function: studies with fluorescent ligands....Pages 45-53 Genome organization and expression of the rat ACBP gene family....Pages 55-61 Regulation of transcription factor mRNA accumulation during 3T3-L1 preadipocyte differentiation by antagonists of adipogenesis....Pages 63-71 Expression of rat L-FABP in mouse fibroblasts: role in fat absorption....Pages 73-83 Research of an in vitro model to study the expression of fatty acid-binding proteins in the small intestine....Pages 85-92 Mechanisms of regulation of liver fatty acid-binding protein....Pages 93-100 Fatty acid-binding protein and its relation to fatty acid oxidation....Pages 101-106 Modulation of fatty acid-binding protein content of adult rat heart in response to chronic changes in plasma lipid levels....Pages 107-112 Expression and localization of intestinal 15 kDa protein in the rat....Pages 113-120 Retinal FABP principally localizes to neurons and not to glial cells....Pages 121-127 The function of acyl-CoA-binding protein (ACBP)/Diazepam binding inhibitor (DBI)....Pages 129-138 Purification and partial characterisation of an α-tocopherol-binding protein from rabbit heart cytosol....Pages 139-144 Role of fatty acid-binding protein in lipid metabolism of insect flight muscle....Pages 145-152 Developmental changes of FABP concentration, expression, and intracellular distribution in locust flight muscle....Pages 153-158 Characterization of the non-specific lipid transfer protein EP2 from carrot ( Daucus carota L.)....Pages 159-166 Significance of cytoplasmic fatty acid-binding protein for the ischemic heart....Pages 167-173 Release of fatty acid-binding protein and long chain fatty acids from isolated rat heart after ischemia and subsequent calcium paradox....Pages 175-184 Fatty acid-binding proteinuria diagnoses myocardial infarction in the rat....Pages 185-190 Cellular binding proteins for fatty acids and retinoids: similar or specialized functions?....Pages 191-202 Back Matter....Pages 203-205 This volume is a collection of selected papers presenting state-of-the-art knowledge of cellular FABPs. The contributors to this issue represent new investigations and also reflect the multidisciplinary nature of research in this rapidly-progressing field.