Cell and Tissue Reaction Engineering : With a Contribution by Martin Fussenegger and Wilfried Weber

The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

Today mammalian cell products, primarily monoclonals, cytokines, recombinant glycoproteins and increasingly vaccines dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells for regenerative medicine have been introduced on the market.

This book elucidates the interactions between biological as well as biochemical and engineering principles in processes derived from cell cultures. The authors explain the basic characteristics of mammalian cells related to cultivation systems and the consequences on design and operation of bioreactor systems, so that the importance of cell culture technology as well as differences compared to microbial fermentation technology are understood. Further, special cell culture applications, such as insect cell-based recombinant protein production, bioartificial organs and plant cell-based bioprocessing, are presented.

This book is of interest to students of biotechnology as well as scientists and industrial users.

Cover 1 Cell and Tissue Reaction Engineering 3 Principles and Practice 2 ISBN: 3540681752 4 Preface 5 Contents 7 Chapter 1 Mammalian Cell Culture Technology: An Emerging Field 12 Chapter 2 Characteristics of Mammalian Cells and Requirements for Cultivation 21 Chapter 3 Bioreactors for Mammalian Cells: General Overview 62 Chapter 4 Special Engineering Aspects 90 Chapter 5 Bioreactor Design and Scale-Up 180 Chapter 6 Insect Cell-Based Recombinant Protein Production 267 Chapter 7 Bioreactors for Bioartificial Organs 282 Chapter 8 Plant Cell-Based Bioprocessing 317 Index 359 ISBN-13:,9783540681755 Springer Today mammalian cell products, primarily monoclonals, cytokines, recombinant glycoproteins and increasingly vaccines dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells for regenerative medicine have been introduced on the market. This book elucidates the interactions between biological as well as biochemical and engineering principles in processes derived from cell cultures. The authors explain the basic characteristics of mammalian cells related to cultivation systems and the consequences on design and operation of bioreactor Front Matter....Pages i-xi Mammalian Cell Culture Technology: An Emerging Field....Pages 3-11 Characteristics of Mammalian Cells and Requirements for Cultivation....Pages 13-53 Bioreactors for Mammalian Cells: General Overview....Pages 55-82 Special Engineering Aspects....Pages 83-172 Bioreactor Design and Scale-Up....Pages 173-259 Insect Cell-Based Recombinant Protein Production....Pages 263-277 Bioreactors for Bioartificial Organs....Pages 279-313 Plant Cell-Based Bioprocessing....Pages 315-356 Back Matter....Pages 357-363