Biotechnological Approaches to Enhance Plant Secondary Metabolites : Recent Trends and Future Prospects

Thousands of secondary metabolites are produced by plants to withstand unfavourable environmental conditions and are important molecules for nutraceutical, agro, cosmetic and pharmaceutical industries, etc. Harvesting of plants for the extraction of these important metabolites can threaten the plant germplasm, and various medicinally important plants are at the verge of extinction. Based on need, various methods and strategies were developed and followed by researchers from time to time to save the plant germplasm and produce important secondary metabolites efficiently to meet their growing demands. Biotechnological Approaches to Enhance Plant Secondary Metabolites: Recent Trends and Future Prospects provides a comprehensive introduction and review of state-of-the-art biotechnological tools in this field of research at global level. The methodologies are highlighted by real data examples in both __in vitro__ and __in vivo__ level studies. The book: • Highlights and provides overviews of the synthesis, classification, biological function and medicinal applications of the recent advancements for the enhanced production of novel secondary metabolites in plants • Provides an overview of the role of induced mutation, salinity stress and brassinosteroids impact to increase the secondary metabolic contents in plants and suggests an increase in enzymatic activity in plants could be due to various point mutations, which in turn could play a role at transcriptome levels • Discusses the significant role of endophytes to enhance the contents of plant secondary metabolites • Alternatively, suggests the urgent need to set up the standard operating procedures using hydroponics system of cultivation for significant enhancement of secondary metabolite contents • Enlists various __in vitro__ techniques to enhance plant secondary metabolites contents using plant tissue culture approaches • Provides a systematic overview of state-of-the-art biotechnological tools CRISPER Cas9 and RNAi to enhance the plant secondary metabolite contents • Recommends CRISPER Cas9 technology over RNAi, ZFNs and TALENs because of its relatively simple and high precision method with an easily programmable tool This serves as a reference book for the researchers working in the field of plant secondary metabolites and pharmaceutical industries at global level. "Thousands of secondary metabolites are produced by the plants to withstand under unfavourable environmental conditions and are reported commercially important molecules for nutraceutical, agro, cosmetic and pharmaceutical industries etc. Harvesting of plants for the extraction of these important metabolites leads to threat the plant germplasm and various medicinally important plants are at the verge of extinction. Based on the needs various methods and strategies were discovered and followed by the researchers from time to time to save the plant germplasm and produce important secondary metabolites efficiently to meet their growing demands. Biotechnological approaches to enhance plant secondary metabolites: recent trends and future prospects provide a comprehensive introduction and review of state-of-the-art of biotechnological tools in this field of research at global level. The methodologies are highlighted by real data examples at both in vitro and in vivo level studies. The book is having the following key features: It highlights the synthesis, classification, biological function, medicinal applications, and an overview of the recent advancements for the enhanced production of novel secondary metabolites in plants. It overviewed the role of induced mutation, salinity stress, Brassinosteroids impact to increase the secondary metabolic contents in plants and suggests increase in enzymatic activity in plants could be due to various point mutations which in turn could play a role at transcriptome levels. It discussed the significant role of endophytes to enhance the contents of plant secondary metabolites. Alternatively, it also suggests urgent need to set up the standard operating procedures using hydroponics system of cultivation for significant enhancement of secondary metabolite contents. It enlists various in vitro techniques to enhance plant secondary metabolites contents using plant tissue culture approaches. It systematically overviewed the state of art in biotechnological tools viz. CRISPER Cas9, and RNAi to enhance the plant secondary metabolite contents and recommend CRISPER Cas9 technology over the RNAi, ZFNs and TALENs because of its relatively simple and high precision method with easily programmable tool by changing the guide sequence of the sgRNA to any DNA sequence of interest with its capacity to recognize multiple targeted genes with very low off-target effects due to reduced CpG methylation. The book will serve as a reference book for the researchers working in the field of plant secondary metabolites and pharmaceutical industries at global level"-- Provided by publisher Cover Half Title Title Page Copyright Page Dedication Contents Preface Acknowledgements Editor Contributors Chapter 1: Plant Secondary Metabolites—The Key Drivers of Plant’s Defence Mechanisms: A General Introduction Chapter 2: Generation of Plant Mutant Lines Using Gamma Radiation with Enhanced Secondary Metabolite Contents Chapter 3: Salinity Stress and Plant Secondary Metabolite Enhancement: An Overview Chapter 4: Enhancement of Plant Secondary Metabolites Using Fungal Endophytes Chapter 5: Hydroponic Cultivation Approaches to Enhance the Contents of the Secondary Metabolites in Plants Chapter 6: Tissue Culture Approaches to Enhance Plant Secondary Metabolites Production Chapter 7: Hairy Roots and Plant Secondary Metabolites Production: An Update Chapter 8: Brassinosteroids: The Phytohormones with Potential to Enhance the Secondary Metabolite Production in Plants Chapter 9: CRISPR-Cas9 Approaches to Enhance Contents of Plant Secondary Metabolites Chapter 10: RNA Interference for Improvement of Secondary Metabolite Production in Plants Index This book provides a comprehensive introduction and review of state-of-the-art of biotechnological tools in this field of research at global level. The methodologies are highlighted by real data examples at both in vitro and in vivo level studies.