Bioproducts and Bioprocesses: Second Conference to Promote Japan/U.S. Joint Projects and Cooperation in Biotechnology, Lake Biwa, Japan, September 27–30, 1986.

The Japanese biotechnology community has developed a unique system of scientific bilateral communications at the international level. It consists of closed seminars where a restricted number of speakers from Japan and a selected country are invited. Thereby the actual state of biotechnology is visualized very effectively and valuable information exchanged during the discussions and personal contacts. As a result strong relationships between several European and American countries have been built up during recent years and numerous colleagues from abroad have improved their knowledge of Japanese science, economy, and culture. The present volume reports on an American-Japanese meeting held in Moriyama, which is situated on the lovely Lake Biwa in Japan. The well prepared and efficiently organized event covered the major aspects of bioprocess engineering including animal and plant cell culture, biocatalysts, downstream processing, sensor and bioprocess control, as well as genetic engineering. Altogether a broad spectrum, indicating the strengths and weaknesses of the current efforts being made for the improvement of process technology and the search for new products. Front Matter....Pages I-XI Front Matter....Pages 1-1 Cultured Plant Cells as the Catalysts for Bioreactor....Pages 3-12 Characteristics and Utility of Human Hematopoietic Cell Lines....Pages 13-29 Scale-up of Oxygen Transfer in Animal Cell Cultures....Pages 31-31 Stoichiometric Considerations of Mammalian Cell Metabolism in Bioreactors....Pages 33-46 Front Matter....Pages 47-47 Microbial and Enzymatic Processes for the Production of Biologically Useful Compounds....Pages 49-50 Inducible Recombinant Cell Cultures and Bioreactors....Pages 51-70 Intradiffusion Effect on Reactivity of Immobilized Microorganisms....Pages 71-85 The Adsorption of Trichoderma reesei C30 Cellulase Protein to Protein Adsorbents: Application to Cellulase Recovery and Reuse....Pages 87-101 The Construction of a Bioreactor for the Production of an Optically Active Compound....Pages 103-112 A New Method of Graphical Analysis of Batch Data for Continuous Operation, the “Tangential Method”....Pages 113-145 Enzymes in Organic Synthesis: Aldol Reactions as a Route to Unusual Sugars....Pages 147-154 Production of L-Phenylalanine Using Enzymes....Pages 155-167 Front Matter....Pages 169-169 Genetic Engineering in Coryneform Bacteria....Pages 171-171 Formation of Jumbo Yeast Cells by Introduction of Methylglyoxal Resistant Genes, Development of New Vector for Wild or Industrially Used Cells....Pages 173-180 Cloning and Over-Expression of the E. coli D-Xylose Isomerase Gene in Various Microorganisms....Pages 181-188 Production of Useful Proteins by Bacillus brevis ....Pages 189-199 Extracellular Secretion of Cloned Serratia marcescens Protease through the Outer Membrane from Escherichia coli Cells....Pages 201-201 Cumulative Effect of Intragenic Amino Acid Replacements on Thermostability of a Protein....Pages 203-203 Front Matter....Pages 205-205 Separation and Purification in Bioprocess....Pages 207-207 Bioseparations of Milk Proteins....Pages 209-221 Front Matter....Pages 205-205 Design and Use of Enzyme Systems for Selective Product Release from Microbial Cells....Pages 223-234 Dynamic In-Situ Fractionation of Extracellular Proteins Produced in a Baker’s Yeast Cultivation Process....Pages 235-255 Supercritical Fluid Bioreactor....Pages 257-265 Application of Affinity Adsorption in Antibiotic Production....Pages 267-277 Application of Cross-Flow Filtration for High Density Cultivation and Regulation of trp Promoter....Pages 279-279 Extraction of Volatile Fatty Acids from Spent Medium with a Supported Liquid Membrane....Pages 281-293 Front Matter....Pages 295-295 Microbiosensors for Clinical and Process Analysis....Pages 297-306 Use of Infrared Spectroscopy for the On-Line Multicomponent Analysis and Control of Bioprocesses....Pages 307-307 The Use of Fluorometry in Monitoring and Control of Cell Cultures....Pages 309-320 Mass Production of Intracellular Metabolite by Fully Automatic Fed-batch Culture of Microorganism....Pages 321-336 A Database System and an Expert System for Realizing Factory Automation in the Bioindustries....Pages 337-346 Recombinant Bioprocess Technology: Is It Different from Traditional Microbial Culture Process Technology....Pages 347-358 Effect of Shear Stress on Xanthan Gum Production....Pages 359-370 The Biological Component of Expert Systems....Pages 371-377 The current state of biotechnology is visualized very effectively. The present volume reports on an American-Japanese meeting held in Moryama, Japan. The well prepared and efficiently organized event covered the major aspects of bioprocess engineering including animal and plant cell culture, biocatalysts, downstream processing, sensoring and bioprocess control, as well as genetic engineering. The broad spectrum indicates strengths and weaknesses of current efforts being made for the improvement of process technology and the search for new products. The contributions collected in this volume have been drafted carefully by the authors since the meeting took place. Thanks to the critical editorial work of Professor Okada from Osaka University and Professor Tanner from Vanderbilt University a most interesting picture of the present day status of bioprocess engineering is made available