Bioproducts and Bioprocesses 2 : Third Conference to Promote Japan/U.S. Joint Projects and Cooperation in Biotechnology, Honolulu, Hawaii, January 6–10, 1991

Introduction During the week of January 6-10, 1991, the Third U.S.-Japan Conference on Biotechnology was held at the Asian-Pacific Conference Center at the Univer­ sity of Hawaii in Honolulu. This book is a compilation of the papers and posters presented at the Conference. The Conference was sponsored, in part, by the U.S. pharmaceutical companies including National Science Foundation and Ortho Pharmaceutical, Merck, Genentech, Smith Kline Beecham and ABEe. Its purpose was to promote information exchange between Japanese and U.S. researchers, primarily academics, in biotechnology and to seek ways to carry out collaborative research in biotechnology. The honorary chairmen of the Conference were Professor H. Okada and me. The formal program was organized by Professors 1. Bailey and T. Yoshida. Twelve invited formal presentations were given from each side. In addition, both sides were invited to bring along five observers to the Conference who were encouraged to prepare poster presentations on their research. Paper abstracts plus bibliographies were exchanged prior to the Conference in order to promote maximum technical interaction between the participants. Front Matter....Pages I-XIII Front Matter....Pages 1-1 Biochemical Engineering and Biotechnology—An NSF Perspective....Pages 3-3 Use of Fluorometry for On-Line Monitoring and Control of Bioreactors — Microbial Cell Concentration and Activity, Plant Cell Metabolism, Mixing Time, and Gas Hold-up....Pages 5-21 Computer Control of Glutamic Acid Production Based on Fuzzy Clustering of Culture Phases....Pages 23-30 National Science Foundation Engineering Centers Division — Biotechnology and Bioengineering Research Centers....Pages 31-32 The Effect of Glutathione Depletion in the Glutamine Synthetase Gene Amplification System....Pages 33-33 Modeling Vapor Phase Water Droplet Extraction of Proteins from the Medium of an Air Fluidized Bioreactor....Pages 35-46 Front Matter....Pages 47-47 Genetic Engineering and Protein Engineering on Chymosin and Mucor Rennin....Pages 49-61 Genetic Engineering of Carbon and Energy Metabolism....Pages 63-66 Microbial Production of Useful Compounds with recDNA Technique....Pages 67-80 Amplification of Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824....Pages 81-88 Cloning and Sequencing of the Alcohol Dehydrogenase Gene from Bacillus stearothermophilus and Alteration of the Optimum pH of the Enzyme by Protein Engineering....Pages 89-102 Bioprocess Kinetics and Optimization of Recombinant Fermentation: Genetic and Engineering Approaches....Pages 103-111 Front Matter....Pages 113-113 Comparison of Primary, Secondary and Tertiary Structures of Xylanase of Bacillus pumilus and Cellulase of Aspergillus acleatus ....Pages 115-122 Synthesis of Chiral Intermediates for D -Pantothenate Production by Microbial Enzymes....Pages 123-133 Mechanistic Aspects of Enzymatic Catalysis in Anhydrous Organic Solvents....Pages 135-135 Construction of a Novel Biocatalyst Based on an Organelle Model—Expression of the Latent Function of the Enzyme by Organized Assemblies....Pages 137-147 Enzyme Catalysis in Unusual Environments....Pages 149-149 Preparation and Characterization of Semisynthetic Oxidases....Pages 151-162 Front Matter....Pages 163-163 On-line Diagnosing System for Fed-Batch Fermentation....Pages 165-165 Sensors for Bioprocess Monitoring and Control....Pages 167-167 Front Matter....Pages 163-163 Development of Micro—Biosensors for Brain Research....Pages 169-175 Application of 2-D Gel Electrophoresis to Study Intracellular Events in Industrial Fermentations....Pages 177-191 A Knowledge-Based Approach for Control of Phenylalanine Production by a Recombinant Escherichia coli ....Pages 193-216 Modulator Sorption in Gradient Elution Chromatography....Pages 217-232 Front Matter....Pages 233-233 Strategies for Improving Productivity in Plant Cell, Tissue, and Organ Culture in Bioreactors....Pages 235-245 Genetic Engineering of Medicinal Plants....Pages 247-254 Engineering Growth Factor/Receptor Processes: Effects of EGF Receptor Trafficking Dynamics on Cell Proliferation Responses To EGF....Pages 255-270 The Effect of Light Irradiation on Secondary Metabolite Production by Coffea arabica Cells....Pages 271-282 A Model for Embryo Development in Dicotyledonous Plants....Pages 283-292 Kinetics of Hybridoma Cell Growth in Continuous Culture....Pages 293-308 Development of New Immobilization Method and Continuous Production of Bioproducts in Immobilized Mammalian Cell System....Pages 309-309 The current state of biotechnology is visualized very effectively in this volume, which reports on an American- Japanese meeting held in Honolulu, Hawaii. This well prepared and efficiently organized event covered all major aspects of bioprocess engineering. The broad spectrum indicates strengths and weaknesses of current efforts being made for the improvement of technology and the search for new products. The contributions in this volume have been carefully rewritten by the authors since the meeting took place. Thanks to the critical editorial work of Professor Yoshida from Osaka University and Professor Tanner from Vanderbilt University a most interesting picture of the present day status of bio-process engineering is made available