Biomembranes, Part M: Transport in Bacteria, Mitochondria, and Chloroplasts: General Approaches and Transport Systems, Volume 125: Volume 125: Biomembranes Part M (Methods in Enzymology)

The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume 125 Pages xi-xv Preface Page xvii Sidney Fleischer, Becca Fleischer Volumes in series Pages xix,xxi-xxx [1] Energetics of proton transport and secondary transport Original Research Article Pages 3-15 Hagai Rottenberg [2] Isolation of highly coupled heart mitochondria in high yield using a bacterial collagenase Original Research Article Pages 16-27 Peter P. Toth, Shelagh M. Ferguson-Miller, Clarence H. Suelter [3] Alkyl glycoside detergents: Synthesis and applications to the study of membrane proteins Original Research Article Pages 27-35 T. Vanaken, S. Foxall-Vanaken, S. Castleman, S. Ferguson-Miller [4] Lipid enrichment and fusion of mitochondrial inner membranes Original Research Article Pages 35-45 Charles R. Hackenbrock, Brad Chazotte [5] Changes of surface and membrane potentials in biomembranes Original Research Article Pages 46-58 Naoki Kamo, Yonosuke Kobatake [6] Rate of redox reactions related to surface potential and other surface-related parameters in biological membranes Original Research Article Pages 58-86 Shigeru Itoh, Mitsuo Nishimura [7] Use of N,N′ -dicyclohexylcarbodiimide to study membrane-bound enzymes Original Research Article Pages 86-108 Maceij J. Nałȩcz, Robert P. Casey, Angelo Azzi [8] Voltammetric measurements of quinones Original Research Article Pages 109-119 Roger C. Prince, Paul Lloyd-Williams, J. Malcolm Bruce, P. Leslie Dutton [9] Determination of partition coefficients of quinones by high-performance liquid chromatography Original Research Article Pages 119-129 Barry S. Braun, Uli Benbow, Paul Lloyd-Williams, J. Malcolm Bruce, P. Leslie Dutton [10] Function of protonmotive force in translocation of protein across membranes Original Research Article Pages 129-138 Linda L. Randall [11] Use of secretion cloning vectors for guiding the localization of proteins in Escherichia coli Original Research Article Pages 138-149 Charles A. Lunn, Masayasu Takahara, Masayori Inouye [12] Use of 1ac gene fusions to study transport proteins Original Research Article Pages 150-156 Howard A. Shuman [13] Use of transposons to isolate and characterize mutants lacking membrane proteins, illustrated by the sugar transport systems of Escherichia coli Original Research Article Pages 157-180 Maurice C. Jones-Mortimer, Peter J.F. Henderson [14] ECF locus in Escherichia coli : Defect in energization for ATP synthesis and active transport Original Research Article Pages 180-186 Jen-Shiang Hong [15] Mutations in the eup locus of Escherichia coli , energy uncoupled phenotype Original Research Article Pages 187-190 Charles A. Plate [16] Methods for mutagenesis of the bacterioopsin gene Original Research Article Pages 190-214 Marie A. Gilles-Gonzalez, Neil R. Hackett, Simon J. Jones, H. Gobind Khorana, Dae-Sil Lee, Kin-Ming Lo, John M. McCoy [17] Oligonucleotide-directed site-specific mutagenesis of the 1ac permease of Escherichia coli Original Research Article Pages 214-230 Hemanta K. Sarkar, Paul V. Viitanen, Etana Padan, William R. Trumble, Mohindar S. Poonian, Warren McComas, H. Ronald Kaback [18] DNA sequence and transcription of genes for ATP synthase subunits from photosynthetic bacteria Original Research Article Pages 230-249 Victor L.J. Tybulewicz, Gunnar Falk, John E. Walker [19] Membrane transport in rickettsiae Original Research Article Pages 253-259 Herbert H. Winkler [20] Transport in mycoplasmas Original Research Article Pages 259-264 Shlomo Rottem, Vincent P. Cirillo [21] Transport through the outer membrane of bacteria Original Research Article Pages 265-278 Hiroshi Nikaido [22] Binding protein-dependent active transport in Escherichia coli and Salmonella typhimurium Original Research Article Pages 279-289 Clement E. Furlong [23] Calcium-induced permeabilization of the outer membrane: A method for reconstitution of periplasmic binding protein-dependent transport systems in Escherichia coli and Salmonella typhimurium Original Research Article Pages 289-302 Johann M. Brass [24] Reconstitution of periplasmic binding protein-dependent glutamine transport in vesicles Original Research Article Pages 302-309 Arthur G. Hunt, Jen-Shiang Hong [25] Isolation and crystallization of bacterial porin Original Research Article Pages 309-328 R. Michael Garavito, Jürg P. Rosenbusch [26] Ion extrusion systems in Escherichia coli Original Research Article Pages 328-336 Barry P. Rosen [27] Intracellular pH regulation in bacterial cells Original Research Article Pages 337-352 Etana Padan, Shimon Schuldiner [28] Regulation of internal pH in acidophilic and alkalophilic bacteria Original Research Article Pages 352-365 T.A. Krulwich, A.A. Guffanti [29] Peptide transport in bacteria Original Research Article Pages 365-377 Christopher F. Higgins, Margaret M. Gibson [30] Methods for the study of the melibiose carrier of Escherichia coli Original Research Article Pages 377-387 Dorothy M. Wilson, Tomofusa Tsuchiya, T. Hastings Wilson [31] Assay, genetics, proteins, and reconstitution of proton-linked galactose, arabinose, and xylose transport systems of Escherichia coli Original Research Article Pages 387-429 Peter J.F. Henderson, Andrew J.S. Macpherson [32] Purification, reconstitution, and characterization of the lac permease of Escherichia coli Original Research Article Pages 429-452 Paul Viitanen, Michael J. Newman, David L. Foster, T. Hastings Wilson, H. Ronald Kaback [33] Preparation of monoclonal antibodies and site-directed polyclonal antibodies against the lac permease of Escherichia coli Original Research Article Pages 453-467 Nancy Carrasco, Doris Herzlinger, Waleed Danho, H. Ronald Kaback [34] Glycerol facilitator in Escherichia coli Original Research Article Pages 467-473 E.C.C. Lin [35] Polyhydric alcohol transport by bacteria Original Research Article Pages 473-485 Joseph W. Lengeler [36] Alanine carrier from thermophilic bacteria Original Research Article Pages 485-491 H. Hirata [37] Generation of a protonmotive force in anaerobic bacteria by end-product efflux Original Research Article Pages 492-510 Bart Ten Brink, Wil N. Konings [38] Light-driven chloride transport in halorhodopsin-containing cell envelope vesicles Original Research Article Pages 510-520 Brigitte Schobert, Janos K. Lanyi [39] Sodium translocation by NADH oxidase of Vibrio alginolyticus : Isolation and characterization of the sodium pump-defective mutants Original Research Article Pages 520-530 Hajime Tokuda [40] Preparation, characterization, and reconstitution of oxaloacetate decarboxylase from Klebsiella aerogenes , a sodium pump Original Research Article Pages 530-540 Peter Dimroth [41] Sodium pump methylmalonyl-CoA decarboxylase from Veillonella alcalescens Original Research Article Pages 540-546 Wilhelm Hilpert, Peter Dimroth [42] Biotin-dependent decarboxylases as bacterial sodium pumps: Purification and reconstitution of glutaconyl-CoA decarboxylase from Acidaminococcus fermentans Original Research Article Pages 547-558 Wolfgang Buckel [43] Anion exchange in bacteria: Reconstitution of phosphate: Hexose 6-phosphate antiport from Streptococcus lactis Original Research Article Pages 558-563 Suresh V. Ambudkar, Peter C. Maloney [44] Proton-driven bacterial flagellar motor Original Research Article Pages 563-581 Robert M. Macnab [45] Sodium-driven flagellar motors of alkalophilic Bacillus Original Research Article Pages 582-592 Yasuo Imae, Hiroshi Matsukura, Syoyu Kobayasi [46] Mitochondrial outer membrane channel (VDAC, Porin) two-dimensional crystals from Neurospora Original Research Article Pages 595-610 Carmen A. Mannella [47] Reconstitution of ADP/ATP translocase in phospholipid vesicles Original Research Article Pages 610-618 Reinhard Krämer [48] Fluorescent nucleotide analogs as active site probes for the ADP/ATP carrier and the uncoupling protein Original Research Article Pages 618-630 Martin Klingenberg [49] Techniques for NMR and ESR studies on the ADP/ATP carrier Original Research Article Pages 631-639 Klaus Beyer, Anton Munding [50] Fluorescent probes of the mitochondrial ADP/ATP carrier protein Original Research Article Pages 639-649 Marc R. Block, François Boulay, Gérard Brandolin, Yves Dupont, Guy J.M. Lauquin, Pierre V. Vignais [51] ADP/ATP carrier: Analysis of transmembrane folding using pyridoxal phosphate Original Research Article Pages 650-658 Werner Bogner, Heinrich Aquila [52] Chemical modifications and active site labeling of the mitochondrial ADP/ATP carrier Original Research Article Pages 658-670 Marc R. Block, François Boulay, Gérard Brandolin, Guy J.M. Lauquin, Pierre V. Vignais [53] Measurement of citrate transport in tumor mitochondria Original Research Article Pages 671-691 Ronald S. Kaplan, Risa A. Parlo, Peter S. Coleman [54] Partial purification and reconstitution of the tricarboxylate carrier from rat liver mitochondria Original Research Article Pages 692-696 Ferdinando Palmieri, Italo Stipani, Girolamo Prezioso, Reinhard Krämer [55] Isolation and reconstitution of the phosphate transport protein from mitochondria Original Research Article Pages 697-705 Hartmut Wohlrab, Hanno V.J. Kolbe, Anne Collins [56] Transport of metabolites across the chloroplast envelope Original Research Article Pages 705-716 H.W. Heldt, U.I. Flügge [57] Chloroplast phosphate-triose phosphate-phosphoglycerate translocator: Its identification, isolation, and reconstitution Original Research Article Pages 716-730 U.I. Flügge, H.W. Heldt Author index Pages 731-759 Subject index Pages 761-788