Bioluminescence And Chemiluminescence: Progress And Perspectives - Proceedings Of The 13th International Symposium

Synopsis''Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence.The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays.'' 9812561188......Page 1 CONTENTS......Page 16 Preface......Page 8 PART 1. BIOLUMINESCENCE......Page 30 MATERIALS AND METHODS......Page 32 RESULTS......Page 33 DISCUSSION......Page 34 REFERENCES......Page 35 INTRODUCTION......Page 36 Fluorinated dehydrocoelenterazine......Page 37 ACKNOWLEDGEMENTS......Page 38 REFERENCES......Page 39 RESULTS AND DISCUSSION......Page 40 REFERENCES......Page 43 INTRODUCTION......Page 44 The four conserved histidines are found in all seven species examined......Page 45 Structure of domain 3 of L. polyedrum giving the location of histidines......Page 46 REFERENCES......Page 47 LUMINESCENCE IN ECHINODERMS......Page 48 LUMINESCENCE IN OPHIUROIDS......Page 49 ACKNOWLEDGMENTS......Page 50 REFERENCES......Page 51 Habitat......Page 52 Cessation of luminescence......Page 53 Luminescence activity......Page 54 REFERENCES......Page 55 Aequorin and GFP: an historical account Shimomura O......Page 56 REFERENCES......Page 62 INTRODUCTION......Page 64 RESULTS AND DISCUSSION......Page 65 ACKNOWLEDGMENTS......Page 66 REFERENCES......Page 67 METHODS......Page 68 Electrophysiology......Page 69 DISCUSSION......Page 70 REFERENCES......Page 71 PART 2. BEETLE BIOLUMINESCENCE......Page 72 INTRODUCTION......Page 74 Concentration dependency of the N-domain activity......Page 75 Sensitive detection of LHz-AMP using the N-domain......Page 76 REFFERENCES......Page 77 INTRODUCTION......Page 78 Dependence of product yield on enzyme and substrate concentration......Page 79 REFERENCES......Page 81 MATERIALS AND METHODS......Page 82 RESULTS AND DISCUSSION......Page 83 REFERENCES......Page 85 EXPERIMENTAL METHODS......Page 86 RESULTS AND DISCUSSION......Page 87 REFERENCES......Page 89 METHODS......Page 90 RESULTS AND DISCUSSION......Page 91 REFERENCES......Page 93 METHODS......Page 94 RESULTS......Page 95 REFERENCES......Page 97 Absorption and fluorescence spectra of MMOL......Page 98 Stability of DMOL and MMOL in buffer solutions......Page 99 Interaction of MMOL and DMOL with the wild-type and mutant luciferases......Page 100 REFERENCES......Page 101 PART 3. MARINE BACTERIA BIOLUMINESCENCE......Page 102 MATERIALS AND METHODS......Page 104 RESULTS AND DISCUSSION......Page 105 REFERENCES......Page 107 THEORY......Page 108 REFERENCES......Page 111 INTRODUCTION......Page 112 RESULTS AND DISCUSSION......Page 113 REFERENCES......Page 115 METHODS......Page 116 RESULTS......Page 117 REFERENCES......Page 119 METHODS......Page 120 RESULTS......Page 121 REFERENCES......Page 123 LUMINOUS BACTERIA AS PRODUCERS OF ENZYMES......Page 124 DATABASE OF CULTURE COLLECTION IBSO......Page 126 REFERENCES......Page 127 INTRODUCTION......Page 128 RESULTS AND DISCUSSION......Page 129 REFERENCES......Page 131 Isolation of P. leiognathi from the light organ of L. rivulatus......Page 132 RESULTS AND DISCUSSION......Page 133 REFERENCES......Page 135 Preparation of supplements......Page 136 Effect of amino acids and chitosan on RLU of RL-1......Page 137 Effect of amino acids and chitosan on RLU of other strains......Page 138 REFERENCES......Page 139 PART 4. CYPRIDINA (VARGULA) BIOLUMINESCENCE......Page 140 INTRODUCTION......Page 142 RESULTS AND DISCUSSION......Page 143 REFERENCES......Page 145 RESULTS AND DISCUSSION......Page 146 REFERENCES......Page 149 MATERIALS AND METHODS......Page 150 RESULTS AND DISCUSSION......Page 151 REFERENCES......Page 153 MATERIALS AND METHODS......Page 154 RESULTS AND DISCUSSION......Page 155 CONCLUSION......Page 156 REFERENCES......Page 157 INTRODUCTION......Page 158 RESULTS AND DISCUSSION......Page 159 REFERENCES......Page 161 PART 5. CHEMILUMINESCENCE......Page 162 INTRODUCTION......Page 164 Reaction of DMD with tertiary amines......Page 165 Effect of DABCO on the singlet oxygen dimol emission......Page 166 REFERENCES......Page 167 METHODS......Page 168 RESULTS AND DISCUSSION......Page 169 REFERENCES......Page 171 Chemiluminescence of the uranium compounds4......Page 172 Chemiluminescence of lanthanide chelates during decomposition of dioxetanes *......Page 173 REFERENCES......Page 175 Apparatus and Procedure......Page 176 RESULTS AND DISCUSSIONS......Page 177 REFERENCES......Page 179 Apparatus and Procedure......Page 180 RESULTS AND DISCUSSION......Page 181 REFERENCES......Page 183 INTRODUCTION......Page 184 RESULTS......Page 185 REFERENCES......Page 187 RESULTS AND DISCUSSIONS......Page 188 REFERENCES......Page 191 RESULTS......Page 192 DISCUSSIONS......Page 194 REFERENCES......Page 195 COMPUTATIONAL METHODS......Page 196 Theoretical interpretation of the large difference in the quantum yield of luminescence between DD and DHA......Page 197 REFERENCES......Page 199 RESULTS AND DISCUSSION......Page 200 REFERENCES......Page 203 CL measurements of indole, I and II......Page 204 Chemiluminescence......Page 205 REFERENCES......Page 207 Apparatus......Page 208 RESULTS......Page 209 REFERENCES......Page 211 RESULTS AND DISCUSSION......Page 212 REFERENCES......Page 214 Materials......Page 216 Measurement of CL spectra (heated samples)......Page 217 Effect of the preservation temperature on chemiluminescence......Page 218 REFERENCES......Page 219 EXPERIMENTAL......Page 220 Metallic salts......Page 221 Application to Hz02 analysis......Page 222 REFERENCES......Page 223 PART 6. 1,ZDIOXETANES......Page 224 RESULTS AND DISCUSSION......Page 226 REFERENCES......Page 229 RESULTS AND DISCUSSION......Page 230 REFERENCES......Page 233 Charge and electron transfer......Page 234 Charge density in PHOD anion......Page 235 HOMO suggests nature of excited state......Page 236 REFERENCES......Page 237 Intramolecular CIEEL mechanism on chemiluminescence of phenoxide substituted 1, 2-dioxetanes Tanaka C, Tanaka J and Matsumoto M......Page 238 REFERENCES......Page 241 PART 7. INSTRUMENTATION & DEVICES......Page 242 Instrumentation......Page 244 RESULTS......Page 245 REFERENCES......Page 247 scanning near-field opWatomic force microscope (SNOWAFM)......Page 248 DNA solution and stretching method......Page 249 Detection of PNA pmbe on DNA molecule......Page 250 REFERENCES......Page 251 INSTRUMENTATION DEVELOPMENT......Page 252 Reagents......Page 253 DISCUSSION......Page 254 REFERENCES......Page 255 PART 8. APPLICATIONS OF LUMINESCENCE......Page 256 INTRODUCTION......Page 258 Measurement of Ara-C activity......Page 259 REFERENCES......Page 260 Reagents and samples......Page 262 Detection......Page 263 RESULTS......Page 264 REFERENCE......Page 265 RESULTS......Page 266 ACKNOWLEDGMENTS......Page 268 REFERENCES......Page 269 Chemicals......Page 270 RESULTS AND DISCUSSION......Page 271 REFERENCES......Page 273 Materials and reagents......Page 274 Optimization of UV irradiation......Page 275 Optimization of CL condition......Page 276 REFERENCES......Page 277 Tagged expression in mammalian cells and cell culture conditions......Page 278 In vitro transcription and translation of Photinam......Page 279 Recombinant Photinam and measurement of light production......Page 280 REFERENCES......Page 281 DESIGN OF FLUORESCENCE PROBES BASED ON PHOTOINDUCED ELECTRON TRANSFER......Page 282 FLUORESCENCE PROBES BASED ON BORON DIPYRROMETHENE......Page 284 REFERENCES......Page 285 HPLC system and chromatographic conditions......Page 286 Derivatization conditions with DIB-CI......Page 287 Method validation......Page 288 REFERENCES......Page 289 METHODS......Page 290 RESULTS......Page 291 REFERENCES......Page 293 Principles......Page 294 Experiments......Page 295 RESULTS......Page 296 REFERENCES......Page 297 Materials......Page 298 RESULTS......Page 299 REFERENCES......Page 301 METHODS......Page 302 RESULTS AND DISCUSSION......Page 304 REFERENCES......Page 305 PART 9. ANTIOXIDANTS, REACTIVE OXYGEN SPECIES & PHAGOCYTOSIS......Page 306 MATERIAL AND METHODS......Page 308 RESULTS......Page 309 ACKNOWLEDGMENTS......Page 311 METHODS......Page 312 Analyte in gaseous phase......Page 313 REFERENCES......Page 315 Neutrophil chemiluminescence......Page 316 RESULTS......Page 317 DISCUSSION......Page 318 REFERENCES......Page 319 MATERIALS AND METHODS......Page 320 RESULTS AND DISCUSSION......Page 321 REFERENCES......Page 323 Isolation of peroxisomes......Page 324 Respiration and ROS generation in mitochondria......Page 325 ROS generation in peroxisomes......Page 326 REFERENCES......Page 327 MATERIALS AND METHODS......Page 328 RESULTS AND DISCUSSION......Page 329 REFERENCES......Page 331 INTRODUCTION......Page 332 RESULTS......Page 333 REFERENCES......Page 334 MATERIALS AND METHODS......Page 336 RESULTS AND DISCUSSION......Page 337 REFERENCES......Page 339 Chemiluminescence measurement......Page 340 RESULTS AND DISCUSSION......Page 341 REFERENCES......Page 343 MATERIALS AND METHODS......Page 344 RESULTS AND DISCUSSION......Page 345 REFERENCES......Page 347 Preparation of sample solution for detection of HzOz in plants extract......Page 348 Antibacterial effect of natural plant extract......Page 349 Generation of Hz02 from natural plant extract......Page 350 REFERENCES......Page 351 All-trans retinoic acid and monomers treatment......Page 352 Cell viability and inhibition of cell growth......Page 353 DISCUSSION......Page 354 REFERENCES......Page 355 EXPERIMENTAL......Page 356 RESULTS AND DISCUSSION......Page 357 REFERENCES......Page 359 MATERIALS AND METHODS......Page 360 RESULTS AND DISCUSSION......Page 361 REFERENCES......Page 363 MATERIALS AND METHODS......Page 364 RESULTS AND DISCUSSION......Page 365 REFERENCES......Page 367 Instrument and measurement principle......Page 368 RESULTS AND DISCUSSION......Page 370 REFERENCES......Page 371 GENERATION METHODS OF SINGLET OXYGEN......Page 372 APPLICATIONS OF SINGLET OXYGEN TO STERILIZATION/ PURIFICATION......Page 373 ACKNOWLEDGEMENTS......Page 374 REFERENCES......Page 375 General procedure for synthesis and analysis of probes......Page 376 RESULTS AND DISCUSSION......Page 377 REFERENCES......Page 379 Subjects, blood collection and PMN isolation......Page 380 RESULTS......Page 381 DISCUSSION AND CONCLUSIONS......Page 382 REFERENCES......Page 383 METHODS......Page 384 RESULTS......Page 385 REFERENCES......Page 387 INTRODUCTION......Page 388 RESULTS AND DISCUSSION......Page 390 REFERENCES......Page 391 PART 10. APPLICATIONS IN MICROBIOLOGY, ECOLOGY, AND ENVIRONMENTAL & FOOD TESTING......Page 392 Phagocytosis of S. Typhimurium DT104 lux' by THP-1 cell......Page 394 Assay to assess phagocytosis of S. Typhimurium DT104 Zux' by THP-1 cells......Page 395 DISCUSSION......Page 396 REFERENCES......Page 397 Construction of self-bioluminescent reporter strains......Page 398 RESULTS AND DISCUSSION......Page 399 REFERENCES......Page 401 Bioluminescent detection......Page 402 Concentration dependent effects of H202 on bioluminescence......Page 403 DMSO Enhances the delivery of H202 and the promotion of bioluminescence......Page 404 REFERENCES......Page 405 INTRODUCTION......Page 406 CONCLUSIONS......Page 407 ACKNOWLEDGMENTS......Page 408 References......Page 409 Bacterial strains and chemical reagents......Page 410 RESULTS AND DISCUSSION......Page 411 REFERENCES......Page 413 MATERIALS AND METHODS......Page 414 RESULTS......Page 415 REFERENCES......Page 417 METHODS......Page 418 RESULTS......Page 419 REFERENCES......Page 421 Inhibiting growth of Escherichia coli 3-5......Page 422 Inhibiting growth of Escherichiu coli......Page 423 Decontaminating water-bloom......Page 424 REFERENCES......Page 425 EXPERIMENTAL......Page 426 RESULTS AND DISCUSSION......Page 427 REFERENCES......Page 429 Sample......Page 430 RESULTS AND DISCUSSION......Page 431 REFERENCES......Page 433 EXPERIMENTAL......Page 434 RESULTS AND DISCUSSION......Page 435 REFERENCES......Page 437 METHODS......Page 438 RESULTS......Page 439 REFERENCES......Page 441 METHODS......Page 442 Bioluminescent ecological monitoring of salt lake......Page 443 System of biosensors for ecological monitoring......Page 444 REFERENCES......Page 445 Instrumentation......Page 446 Methods......Page 447 RESULTS......Page 448 REFERENCES......Page 449 GLASS MICROCHIP FOR TRANSITION METAL ION ANALYSIS......Page 450 A MICROCHIP BASED ON THE OXALATE-H202 CL SYSTEM......Page 451 SEPARATION AND CL DETECTION OF DOPAMINE AND CATECHOL......Page 452 REFERENCES......Page 453 IMPROVED ATP REAGENTS......Page 454 EXTRACTION OF INTRACELLULAR ATP......Page 455 ALTERNATIVES TO TRADITIONAL ATP ASSAYS......Page 456 REFERENCES......Page 457 INTRODUCTION......Page 458 RESULTS & DISCUSSION......Page 459 REFERENCES......Page 461 BIOLUMINESCENT BIOREPORTER INTEGRATED CIRCUITS (BBICS)......Page 462 BBIC SENSING OF THE CHEMICAL ENVIRONMENT......Page 463 BIOREPORTER SENSING OF BIOLOGICAL AGENTS......Page 464 REFERENCES......Page 465 INTRODUCTION......Page 466 Detection of bioluminescence in BOD standard solutions......Page 467 REFERENCES......Page 468 INTRODUCTION......Page 470 Improvement of the HPLC system......Page 471 Application to airborne particulates......Page 472 REFERENCES......Page 473 METHODS......Page 474 RESULTS......Page 476 REFERENCES......Page 477 A. NASA APPLICATIONS OF THE ATP-BASED ASSAY......Page 478 B. ADVANTAGES AND DISADVANTAGES......Page 480 REFERENCES......Page 481 Measurement of emission spectra......Page 482 Imaging of ROS, hydrogen donor and mediator......Page 483 Application to food analysis......Page 484 REFERENCES......Page 485 PART 11. LUMINESCENT IMMUNOASSAYS......Page 486 INTRODUCTION......Page 488 Simultaneous BL-EIA for BDNF and NT-4/5......Page 489 REFERENCES......Page 490 Calcium-regulated photoprotein obelin as a label in immunoassay: an outlook for applications Frank LA, Borisova Wand Vysotski ES......Page 492 REFERENCES......Page 495 Assay procedure......Page 496 Cross-reactivity......Page 497 Method comparison......Page 498 REFERENCES......Page 499 Tandem BL-EIA for Angiotensin I and Endothelin-1......Page 500 RESULTS AND DISCUSSION......Page 501 REFERENCE......Page 503 A HIGH-THROUGHPUT METHOD FOR DEVELOPMENT OF FRET- BASED INDICATORS FOR PROTEOLYSIS*......Page 504 EXPANDED DYNAMIC RANGE OF FRET-BASED Ca^ INDICATORS BY CIRCULARLY PERMUTED YELLOW FLUORESCENT PROTEINS......Page 505 REFERENCES......Page 507 INTRODUCTION......Page 508 Assay Format......Page 509 RESULTS AND DISCUSSION......Page 510 REFERENCES......Page 511 METHODS & RESULTS......Page 512 REFERENCES......Page 515 METHODS & RESULTS......Page 516 REFERENCES......Page 519 MATERIALS AND METHODS......Page 520 RESULTS AND DISCUSSION......Page 521 REFERENCES......Page 523 Avidin-biotin chemiluminescence immunoassay procedure......Page 524 The optimization of solid phase conditions......Page 525 Comparison with Radioimmunoassay (RIA)......Page 526 REFERENCES......Page 527 PART 12. LUMINESCENT ASSAYS FOR ENZYMES, SUBSTRATES, INHIBITORS & CO-FACTORS......Page 528 MATERIALS AND METHODS......Page 530 RESULTS AND DISCUSSION......Page 531 REFERENCES......Page 533 Reagents and solutions......Page 534 Optimization of assay condition......Page 535 CONCLUSION......Page 536 REFERENCES......Page 537 Simultaneous bioluminescent assay for aequorin and biotinylated luciferase......Page 538 Simultaneous bioluminescent assay for aequorin and biotinylated luciferase......Page 539 REFERENCES......Page 541 INTRODUCTION......Page 542 Principles for accumulated light detection by HRP catalyzed imidazole CL......Page 543 REFERENCES......Page 544 PART 13. LUMINESCENT DNA PROBE, GENE EXPRESSION & REPORTER GENE ASSAYS......Page 546 PCR......Page 548 RESULTS AND DISCUSSION......Page 549 Bioluminescent detection of Allele specific PCR products......Page 550 ACKNOWLEDGEMENTS......Page 551 INTRODUCTION......Page 552 RESULTS......Page 553 REFERENCES......Page 555 METHODS......Page 556 RESULTS......Page 557 DISCUSSION......Page 558 REFERENCES......Page 559 INTRODUCTION......Page 560 RESULTS......Page 561 CONCLUSION......Page 562 REFERENCES......Page 563 METHODS......Page 564 Efficiency of split Renillu luciferase complementation......Page 565 Time course of the interaction between Y941 and SH2n......Page 566 REFERENCES......Page 567 Reapents:......Page 568 RESULTS......Page 569 REFERENCES......Page 571 MATERIALS AND METHODS......Page 572 DISCUSSION......Page 573 ACKNOWLEDGEMENTS......Page 574 REFERENCES......Page 575 METHODS......Page 576 RESULTS......Page 577 DISCUSSIONS......Page 578 REFERENCES......Page 579 Index......Page 580 Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence. The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays. The proceedings have been selected for coverage in : Biochemistry & Biophysics Citation Index[symbol]; Chemistry Citation Index[symbol]; Index to Scientific & Technical Proceedings[symbol] (ISTP[symbol] / ISI Proceedings); Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings); CC Proceedings - Engineering & Physical Sciences; and CC Proceedings - Biomedical, Biological & Agricultural Sciences Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence.The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence.The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays.The proceedings have been selected for coverage in:• Biochemistry & Biophysics Citation IndexTM• Chemistry Citation IndexTM• Index to Scientific & Technical Proceedings® (ISTP® / ISI Proceedings)• Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings)• CC Proceedings — Engineering & Physical Sciences• CC Proceedings — Biomedical, Biological & Agricultural Sciences Edited By Akio Tsuji ... [et Al.]. Isbc 2004. These Are The Proceedings Of The 13th International Symposium On Bioluminescence And Chemiluminescence Held At Pacifico Yokohama, Yokohama, Japan From 2 - 6 August 2004.--pref. Includes Bibliographical References And Index.