Bioluminescence and chemiluminescence : light emission ; biology and scientific applications ; proceedings of the 15th International Symposium, Shanghai, 13-17 May 2008

In the last decade, great advances have been made in fundamental research and in the applications of bioluminescence and chemiluminescence. These techniques have become vital tools for laboratory analysis. Bioluminescence imaging has emerged as a powerful new optical imaging technique, offering real-time monitoring of spatial and temporal progression of biological processes in living animals. Bioluminescence resonance energy transfer (BRET) methodology has also emerged as a powerful technique for the study of protein-protein interactions. Luciferase reporter gene technology facilitates monitoring of gene expression and is used to probe molecular mechanisms in the regulation of gene expression. Chemiluminescence detection and analysis have also found diverse applications in life science research; for example, chemiluminescent labels and substrates are now widely used in immunoassay and nucleic acid probe-based assays. The latest advances in this exciting field, from fundamental research to cutting-edge applications, are explored in this most recent volume of the "Biannual Symposium" series, the "Proceedings of the 15th International Symposium on Bioluminescence and Chemiluminescence". The volume highlights advances in fundamental knowledge of luciferase-based bioluminescence, photoprotein-based bioluminescence, fundamental aspects and applications of chemiluminescence, luminescence imaging, fluorescence quantum dots and other inorganic fluorescent materials, phosphorescence and ultraweak luminescence, and instrumentation for measurement and imaging of luminescence. CONTENTS......Page 12 Preface......Page 6 Introduction to the 15th Symposium......Page 10 PART 1. BASIC BIOLUMINESCENCE......Page 26 INTRODUCTION......Page 28 REFERENCES......Page 37 INTRODUCTION......Page 40 RESULTS AND DISCUSSION......Page 41 REFERENCES......Page 43 MATERIALS AND METHODS......Page 44 RESULTS AND DISCUSSION......Page 45 REFERENCES......Page 47 MATERIAL AND METHODS......Page 48 RESULTS AND DISCUSSION......Page 49 REFERENCES......Page 51 MATERIALS AND METHODS......Page 52 RESULTS AND DISCUSSION......Page 53 REFERENCES......Page 55 METHODS......Page 56 RESULTS AND DISCUSSION......Page 57 REFERENCES......Page 59 MATERIALS AND METHODS......Page 60 RESULTS AND DISCUSSION......Page 61 REFERENCES......Page 63 METHODS......Page 64 RESULTS AND DISCUSSION......Page 65 REFERENCES......Page 67 MATERIALS AND METHODS......Page 68 RESULTS AND DISCUSSION......Page 69 REFERENCES......Page 71 WEB RESOURCE......Page 72 REFERENCES......Page 75 INTRODUCTION......Page 76 RESULTS AND DISCUSSION......Page 77 REFERENCES......Page 78 MATERIALS AND METHODS......Page 80 RESULTS......Page 81 CONCLUSION......Page 82 REFERENCES......Page 83 MATERIALS AND METHOD......Page 84 RESULTS AND DISCUSSION......Page 85 REFFERENCES......Page 87 BIOPHOTON EMISSION AS ODD AND EVEN COHERENT STATES......Page 88 REFERENCES......Page 91 INTRODUCTION......Page 92 RESULTS......Page 93 REFERENCES......Page 95 INTRODUCTION......Page 96 RESULTS AND DISCUSSION......Page 97 REFERENCES......Page 99 Mechanism responsible for the spectral differences in firefly bioluminescence UgarovaNN......Page 100 REFERENCES......Page 103 INTRODUCTION......Page 104 RESULTS AND DISCUSSION......Page 105 REFERENCES......Page 107 MATERIALS......Page 108 RESULTS AND DISSCUSION......Page 109 REFERENCES......Page 111 PART 2. APPLIED BIOLUMINESCENCE......Page 112 MATERIALS AND METHODS......Page 114 RESULTS......Page 115 REFERENCES......Page 117 INTRODUCTION......Page 118 RESULTS AND DISCUSSION......Page 119 REFERENCES......Page 121 RESULTS AND DISCUSSION......Page 122 REFERENCES......Page 125 MATERIALS AND METHODS......Page 126 REFERENCES......Page 129 MATERIALS AND METHODS......Page 130 DISCUSSION......Page 132 REFERENCES......Page 133 MATERIAL AND METHODS......Page 134 RESULTS AND DISCUSSION......Page 135 CONCLUSIONS......Page 136 REFERENCES......Page 137 PART 3. BASIC CHEMILUMINESCENCE......Page 138 MATERIALS AND METHOD......Page 140 RESULTS AND DISCUSSION......Page 141 REFERENCES......Page 143 COMPUTATIONAL METHOD......Page 144 RESULTS AND DISCUSSIONS......Page 145 REFERENCES......Page 147 MATERIALS AND METHODS......Page 148 RESULTS AND DISCUSSION......Page 149 REFERENCES......Page 151 INTRODUCTION......Page 152 EXPERIMENTAL......Page 153 RESUL TS AND DISCUSSION......Page 154 REFERENCES......Page 155 INTRODUCTION......Page 156 RESULTS AND DISCUSSION......Page 157 REFERENCES......Page 159 INTRODUCTION......Page 160 RESULTS......Page 161 REFERENCES......Page 163 RESULTS AND DISCUSSION......Page 164 REFERENCES......Page 167 RESULTS......Page 168 REFERENCES......Page 171 MATERIALS AND METHODS......Page 172 RESULTS AND DISCUSSION......Page 173 REFERENCES......Page 175 RESULTS AND DISCUSSION......Page 176 REFERENCES......Page 179 PART 4. APPLIED CHEMILUMINESCENCE......Page 180 BIOSPECIFIC RECOGNITION SYSTEMS......Page 182 ANALYTICAL DEVICES......Page 183 MICROFLUIDICS......Page 184 REFERENCES......Page 185 INTRODUCTION......Page 186 REFERENCES......Page 196 RESULTS AND DISCUSSION......Page 198 REFERENCES......Page 201 EXPERIMENTAL......Page 202 RESULTS AND DISCUSSION......Page 203 CONCLUSIONS......Page 204 REFERENCES......Page 205 INTRODUCTION......Page 206 MATERIALS AND METHODS......Page 207 RESULTS AND DISCUSSION......Page 208 REFERENCES......Page 209 MATERIALS AND METHODS......Page 210 RESULTS AND DISCUSSION......Page 211 REFERENCES......Page 213 MATERIALS AND METHODS......Page 214 RESULTS......Page 215 REFERENCES......Page 217 MATERIALS AND METHODS......Page 218 RESULTS......Page 219 REFERENCES......Page 221 MATERIALS AND METHODS......Page 222 RESULTS AND DISCUSSION......Page 224 REFERENCES......Page 225 MATERIALS AND METHODS......Page 226 RESULTS AND DISCUSSION......Page 227 ACKNOWLEDGEMENT......Page 228 REFERENCES......Page 229 EXPERIMENTAL......Page 230 RESULTS AND DISCUSSION......Page 231 REFERENCES......Page 233 EXPERIMENTAL......Page 234 RESULTS AND DISCUSSION......Page 235 REFERENCES......Page 237 EXPERIMENTAL......Page 238 RESUL TS AND DISCUSSION......Page 239 REFERENCES......Page 241 EXPERIMENTAL......Page 242 REFERENCES......Page 244 MATERIALS AND METHODS......Page 246 RESULTS AND DISCUSSION......Page 247 REFERENCES......Page 249 METHODS......Page 250 RESULTS AND DISCUSSION......Page 251 REFERENCES......Page 253 EXPERIMENTAL......Page 254 RESULTS AND DISCUSSION......Page 255 REFERENCES......Page 257 MATERIALS AND METHODS......Page 258 RESULTS AND DISCUSSION......Page 259 REFERENCES......Page 261 INTRODUCTION......Page 262 REFERENCES......Page 265 MATERIALS AND METHODS......Page 266 RESULTS......Page 267 DISCUSSION......Page 268 REFERENCES......Page 269 MATERIALS & METHODS......Page 270 RESULTS AND DISCUSSION......Page 271 CONCLUSION......Page 272 REFERENCES......Page 273 MATERIALS AND METHODS......Page 274 RESULTS AND DISCUSSION......Page 275 REFERENCES......Page 277 MATERIALS AND METHODS......Page 278 RESULTS AND DISCUSSION......Page 279 REFERENCES......Page 281 METHODS......Page 282 RESULTS......Page 283 REFERENCES......Page 285 THEORETICAL BACKGROUNDS......Page 286 MECHANISM OF CHEMI- AND BIO-LUMINESCENCE REACTIONS......Page 287 REFERENCES......Page 289 RESULTS AND DISCUSSION......Page 290 REFERENCES......Page 293 MATERIALS AND METHODS......Page 294 RESULTS AND DISCUSSION......Page 295 REFERENCES......Page 297 EXPERIMENTAL......Page 298 RESULTS AND DISCUSSION......Page 300 REFERENCES......Page 301 MATERIALS AND METHODS......Page 302 RESULTS AND DISCUSSION......Page 303 REFERENCES......Page 305 RESULTS......Page 306 REFERENCES......Page 310 MATERIALS AND METHODS......Page 312 RESULTS AND DISCUSSION......Page 313 REFERENCES......Page 315 MATERIALS AND METHODS......Page 316 RESULTS......Page 317 REFERENCES......Page 319 PART 5. APPLIED ELECTROLUMINESCENCE......Page 320 EXPERIMENTAL......Page 322 RESULTS AND DISCUSSION......Page 323 REFERENCES......Page 324 MATERIALS AND METHODS......Page 326 RESULTS AND DISCUSSION......Page 327 REFERENCES......Page 329 INTRODUCTION......Page 330 RESUL TS AND DISCUSSION......Page 331 REFERENCES......Page 333 PART 6. BIOMEDICAL APPLICATION OF FLUORESCENT PROTEINS......Page 334 RESULTS AND DISCUSSION......Page 336 REFERENCES......Page 339 RESULTS......Page 340 REFERENCES......Page 343 MATERIALS AND METHODS......Page 344 RESULTS......Page 346 REFERENCES......Page 347 PART 7. DEVELOPMENT AND BIOMEDICAL APPLICATIONS OF QUANTUM DOTS AND OTHER INORGANIC FLUORESCENT MATERIALS......Page 348 EXPERIMENTAL......Page 350 RESULT AND DISCUSSION......Page 351 REFERENCES......Page 353 RESULTS AND DISCUSSION......Page 354 REFERENCES......Page 357 RESULTS AND DISCUSSION......Page 358 REFERENCES......Page 361 PART 8. BIOLUMINESCENCE, CHEMILUMINESCENCE AND FLUORESCENCE IMAGING......Page 362 MATERIALS AND METHODS......Page 364 RESULTS AND DISCUSSION......Page 365 REFERENCES......Page 367 INTRODUCTION......Page 368 RESULTS......Page 369 REFERENCES......Page 370 MATERIALS AND METHODS......Page 372 RESULTS AND DISCUSSION......Page 373 REFERENCES......Page 375 MATERIALS AND METHODS......Page 376 RESULTS AND DISCUSSION......Page 378 REFERENCES......Page 379 MATERIALS AND METHODS......Page 380 REFERENCES......Page 383 MATERIALS AND METHODS......Page 384 RESULTS AND DISCUSSION......Page 385 REFERENCES......Page 387 MATERIALS AND METHODS......Page 388 REFERENCES......Page 390 RESULTS AND DISCUSSION......Page 392 REFERENCES......Page 394 PART 9. ASPECTS OF FLUORESCENCE AND PHOSPHORESCENCE......Page 396 EXPERIMENTAL......Page 398 RESULTS AND DISCUSSION......Page 399 REFERENCES......Page 400 Fluorescence enhancement of KI for the morin-fsDNA system and its analytical application Ding H, Wu X, Yang J and Wang F......Page 402 REFERENCES......Page 404 MATERIALS AND METHODS......Page 406 RESULTS AND DISCUSSION......Page 407 REFERENCES......Page 408 EXPERIMENTAL......Page 410 RESULTS AND DISCUSSION......Page 411 REFERENCES......Page 412 EXPERIMENTAL......Page 414 RESUL TS AND DISCUSSION......Page 415 REFERENCES......Page 417 EXPERIMENTAL......Page 418 RESULTS AND DISCUSSION......Page 419 REFERENCES......Page 421 EXPERIMENTAL......Page 422 RESULTS AND DISCUSSION......Page 423 REFERENCES......Page 424 EXPERIMENTAL......Page 426 RESULTS AND DISCUSSION......Page 428 REFERENCES......Page 429 MATERIAL AND METHODS......Page 430 RESULTS AND DISCUSSION......Page 431 REFERENCES......Page 432 Mechanism and properties of bio-photon emission and absorption of protein molecules in living systems Pang X-F......Page 434 REFERENCES......Page 439 The mechanism of photon emission of bio-tissues and its properties Pang X-F and Cao X-Y......Page 440 REFERENCES......Page 445 EXPERIMENTAL SECTION......Page 446 RESULTS AND DISCUSSION......Page 447 REFERENCES......Page 448 MATERIALS AND METHODS......Page 450 RESUL TS AND DISCUSSION......Page 451 ACKNOWLEDGEMENT......Page 452 REFERENCES......Page 453 RESULTS AND DISCUSSION......Page 454 REFERENCES......Page 457 MATERIALS AND METHODS......Page 458 RESULTS AND DISCUSSION......Page 459 REFERENCES......Page 461 MATERIALS AND METHODS......Page 462 REFERENCES......Page 464 MATERIALS AND METHODS......Page 466 CONCLUSIONS......Page 468 REFERENCES......Page 469 RESULTS......Page 470 DISCUSSION......Page 472 REFERENCES......Page 473 EXPERIMENTAL......Page 474 RESULTS AND DISCUSSION......Page 475 REFERENCES......Page 477 MATERIALS AND METHODS......Page 478 RESULTS AND DISCUSSION......Page 479 CONCLUSION......Page 480 REFERENCES......Page 481 MATERIALS AND METHODS......Page 482 RESULTS AND DISCUSSION......Page 483 REFERENCES......Page 484 MATERIALS AND METHODS......Page 486 RESULTS AND DISCUSSIONS......Page 487 REFERENCES......Page 489 INTRODUCTION......Page 490 RESULTS AND DISCUSSION......Page 491 REFERENCES......Page 493 Index......Page 494 Pt. 1. Basic bioluminescence. Plenary lecture - Progress, perspectives and problems in basic aspects of bioluminescence / Hastings JW. Bioluminescence of sharks, a case study : Etmopterus spinax / Claes JM and Mallefet J. Chemiexcitation mechanism for Cypridina (Vargula) and Aequorea bioluminescence / Hirano T [und weitere]. Site-directed mutagenesis of Lampyris turkestanicus luciferase : The effect of conserved residue(s) in bioluminescence emission spectra among firefly luciferases / Hosseinkhani S [und weitere]. Chemiluminescent and bioluminescent analysis of plant cell responses to reactive oxygen species produced by a new water conditioning apparatus equipped with titania-coated photo-catalytic fibers / Kagenishi T [und weitere]. pH-tolerant mutants of Luciola mingrelica luciferase created by random mutagenesis / Koksharov MI and Ugarova NN. Bacterial bioluminescence with flavinmononucleotide activated by N-methylimidazole / Krasnova OI, Tyulkova NA and Doroshenko IO. New method of measuring bacterial bioluminescence / Krasnova OI, Tyulkova NA and Doroshenko IO. Enhancement of thermostability of Luciola mingrelica firefly luciferase by mutagenesis of non-conservative residues CYS62 and CYS146 / Lomakina GY, Modestova YA and Ugarova NN. Web-resource : "Bioluminescence and luminous organisms" of the IBSO culture collection / Medvedeva SE, Kotov DA and Rodicheva EK. Chemistry of symplectin bioluminescence with fluorodehydrocoelenterazine / Nakashima Y [und weitere]. Mechanisms of heavy atom effect in bioluminescent reactions / Nemtseva EV [und weitere]. Theoretical analysis on the absorption spectra of intermediates of firefly luciferin in deoxygenated dimethyl sulfoxide / Sakai Hand and Wada N. Biophoton emission of biological systems in terms of odd and even coherent states / Kun SI, Liu C and Jia H-Y. Study on ATP-dependent luminescence reaction of the arm light organs of the luminous squid Watasenia scintillans / Teranishi K and Shimomura O. Mechanism of bacterial luciferase : Energetic and quantum yield considerations / Tu S-C. Mechanism responsible for the spectral differences in firefly bioluminescence / Ugarova NN. Luminous mushrooms / Vydryakova GA [und weitere]. Use of Cypridina luciferin analog for assessing the monoamine oxidase-like superoxide-generating activities of two peptide sequences corresponding to the helical copper-binding motif in human prion protein and its model analog / Yokawa K, Kagenishi T and Kawano T -- pt. 2. Applied bioluminescence. Bioluminescent assay of antibiotic susceptibility of clinical samples / Frundzhyan VG and Ugarova NN. BART : Smart biochemistry, bright bioluminescence, low-cost hardware / Gandelman GA [und weitere]. BART applications in medical and food diagnostics / Gandelman GA [und weitere]. Change of expression efficiency of natural and cloned lux-operon in conditions of famine / Gusev AA. Construction of recombinant luminescence bacteria vector to evaluate genetoxic environmental pollutants / Huang X-X [und weitere]. Development of a novel bioluminescent assay for nitric oxide by using soluble guanylate cyclase / Sano Y [und weitere] -- pt. 3. Basic chemiluminescence. Mass spectrometric approach to elucidation of chemiexcitation of dioxetanes / Ijuin HK [und weitere]. Theoretical considerations on the roles of hydrogen bonding in thermal decomposition of peroxides / Isobe H [und weitere]. A new bright chemiluminescent reaction : Interaction of acetone with solid-phase potassium monoperoxysulfate in the complex of europium nitrate / Kazakov DV [und weitere]. Study of novel aryloxalate chemiluminescence reaction without addition of hydrogen peroxide / Kishikawa N [und weitere]. Nucleophilic acylation catalysts effect on luminol chemiluminescence / Marzocchi E [und weitere]. Effect of surfactants on peroxyoxalate chemiluminescence reaction / Nakashima K [und weitere]. Solvent-promoted chemiluminescent decomposition of bicyclic dioxetanes bearing a 4-(benzothiazol-2-yl)-3-hydroxyphenyl / Tanimura M [und weitere]. Synthesis and characterization of near-infrared chemiluminescent probes / Teranishi K. Generation of high-energy chemiluminophores in ambient light / Tsaplev Yu B, Vasil'ev RF and Trofimov AV. Alkaline metal ion enhanced chemiluminescence of bicyclic dioxetanes bearing a 3-hydroxynaphthalen-2-yl group / Watanabe N [und weitere] -- pt. 4. Applied chemiluminescence. Plenary lecture - analytical challenges for luminescence-based point-of-care testing devices in biomedical diagnostics / Roda A [und weitere]. Plenary lecture - molecular imprinted polymer-based chemiluminescence sensors / Zhang Z. Flow injection chemiluminescence determination of hydroxylamine hydrochloride / Baezzat MR and Izadpanah M. Study on gold-sensitised chemiluminescence for the determination of norfloxacin / Bao J-F, Jiang Z-H and Yu X-J. Conjugates of (acridinium)x-BSA-anti-HCV core to enhance the detection of HCV core antigen / Chang CD [und weitere]. Chemiluminescence determination of rutin based on a micelle-sensitizing N-bromosuccinimide-H[symbol]O[symbol] reaction / Du JX, Hao L and Lu JR. Luminol-dependent chemiluminescence increases with formation of phenothiazine cation radicals by horseradish peroxidase / Hadjimitova VA, Traykov T and Bakalova R. Variety of chemiluminescent methods for antioxidant activity : Investigation of Crataegus oxicantha extract / Hadjimitova VA, Traykov T and Bakalova R. Simultaneous multiplex bio- and chemiluminescent enzyme immunoassay for PCR products derived from genetically modified Papaya / Ito K [und weitere]. Effect of sugars on aluminum-induced oxidative burst and cell death in suspensions of tomato cells / Kadono T [und weitere]. Chemiluminescence determination of sparfloxacin using Ru(bipy)[symbol]-Ce(IV) system / Karim MM [und weitere]. Flow injection analysis with chemiluminescence detection : Determination of gatifloxacin using the KMn[symbol]-formaldehyde system / Khan MA, Alam SM and Lee SH. Determination of ciprofloxacin in pharmaceutical formulation by chemiluminescence method / Khan MA [und weitere]. Chemiluminescence flow-through biosensor for hydrogen peroxide based on enhanced HRP activity by gold nanoparticles / Lan D and Li B. Flow injection chemiluminescence determination of thiamine by the enhancement of luminol-K[symbol]Fe(CN)[symbol] system / Li YH, Yang Y and Lu JR. Chemiluminescent and electron spin resonance spectroscopic measurements of reactive oxygen species generated in water treated with Titania-coated photocatalytic fibers / Lin C [und weitere]. A sensitive micellar-enhanced chemiluminescence method for the determination of ofloxacin by flow injection analysis / Ma H [und weitere]. Excessive extracellular chemiluminescence and necrosis of neutrophils in bovine neonates and potentially supportive role of vitamin C / Mehrzad J, Mohri M and Burvenich C. Chemiluminescence of 9-benzylidene-lO-methylacridans with electron-donating groups by chemically generated singlet oxygen - Application to metal ion sensing using azacrowned compound / Motoyoshiya J [und weitere]. Effects of l,4-butanediol dimethacrylate on HL-60 cells metabolism / Nocca G [und weitere]. Determination of pyrogallol by imidazole chemiluminescence enhanced with hydrogen peroxide / Nozaki O [und weitere]. Chemiluminescence study on the regulation of NADPH oxidase activity by thioredoxin reductase in vascular endothelial cells / Shen X and Liu Z-B. Quantitative detection of singlet oxygen with a chemiluminescence probe during photodynamic reactions / Wei Y [und weitere]. Flow-injection chemiluminescence determination of human serum albumin based on fluoresceinyl Cypridina luciferin analog-[symbol]O[symbol] reaction / Xu W [und weitere]. Charge-transfer-induced luminescence (CTIL) mechanisms of chemi- and bioluminescence reactions / Yamaguchi K [und weitere]. A novel synergistic enhancer for HRP-Luminol-H[symbol]O[symbol] based chemiluminescence and its application in immunoassay / Yang X and Sun X Quantum dots as fluorescent resonance energy transfer donors in antibody-antigen systems / Hu S [und weitere]. Synthesis and photoluminescence of green-emitting X[symbol] phosphor under VUV excitation / Zhang ZH, Wang YH and Li XX. Luminescent properties of Na[symbol] nano-sized phosphor / Zhou L-Y [und weitere] -- pt. 8. Bioluminescence, chemiluminescence and fluorescence imaging. The measurement of cytosolic ATP during apoptosis : Bioluminescence imaging at the single cell level / Akiyoshi R and Suzuki H. Bioluminescence imaging of bacteria-host interplay : Interaction of E. coli with epithelial cells / Brovko LY [und weitere]. Ultrasensitive chemiluminescent immunochemical localisation of protein components in painting cross-sections / Dolci LS [und weitere]. Development of a new device for ultrasensitive electrochemiluminescence microscope imaging / Dolci LS [und weitere]. Visualization of sequential response in intra cellular signal transduction cascade by fluorescence and luminescence imaging in the same living cell / Hatta-Ohashi Y, Takahashi T and Suzuki H. Bioluminescence imaging of intracellular calcium dynamics by the photoprotein obelin / Thet MM, Sugiyama T and Suzuki H. Applications of delayed fluorescence and laser confocal scanning microscope techniques in monitoring artificial acid rain stress on plants / Zhang H, Wen F and Zhou X. Delayed fluorescence and optical molecule imaging techniques for detecting the stress response of plants to high temperature / Zhang L and Wen F -- pt. 9. Aspects of fluorescence and phosphorescence. The interaction of Tb[symbol]-protocatechuic acid complex with nucleic acids and its application in determination of nucleic acids based on fluorescence quenching / Chen Y, Yang Y and Yang J. Fluorescence enhancement of KI for the morin-fsDNA system and its analytical application / Ding H [und weitere]. Microemulsion sensitized determination of BSA with 3-(4'-methylphenyl)-5-(2'-sulfophenylazo) rhodanine by resonance Rayleigh scattering method / Ge S [und weitere]. Fluorimetric determination of rutin using rutin-Fe(IlI) system / Karim MM [und weitere]. Micelle enhanced fluorimetric determination of benserazide in pharmaceutical formulations / Lee SH [und weitere]. Improvement in carbaryl assay by fluorescence in a micellar medium / Lee SH [und weitere]. Study of the interaction between human serum albumin and 7-ethyl-1O-hydroxycamptothecin / Li G and Liu Y. Resonance Rayleigh scattering method for determination of alginic sodium diester with methylene blue / Liu Y and Li G. Effects of metal ions on peroxynitrite nitrifying protein / Luo Y [und weitere]. Mechanism and properties of bio-photon emission and absorption of protein molecules in living systems / Pang X-F. The mechanism of photon emission of bio-tissues and its properties / Pang X-F and Cao X-Y. Synthesis of a novel fluorescence probe of [symbol]-CD and cuprous iodide pyridine and its application / Qiao J [und weitere]. Phosphorescence properties of 2-bromoquinoline-3-boronic acid in sodium deoxycholate and its potential application in recognition of carbohydrates / Shen QJ [und weitere]. Study on the interaction between methyl blue and HSA in the presence of [symbol]-CD/HP-[symbol]-CD by molecular spectroscopy / Song S [und weitere]. Study on the interaction of kaempferol with human serum albumin by spectroscopy and molecular modeling / Tian J [und weitere]. Selection of salt-tolerant rice variety using light-induced delayed fluorescence / Wang J [und weitere]. Effects of LMWOA on biodegradation of phenanthrene studied by fluorimetry / Wei XY [und weitere]. Alleviation effects of salicylic acid and lanthanum on ultra weak bioluminescence in maize leaves under cadmium stress / Wei ZL [und weitere]. Rhodamine B-quinoline-8-amide as a fluorescent "ON" probe for Fe[symbol] in acetonitrile / Xiang Y, Li ZF and Tong AJ. Studies on determination of deoxyribonucleic acid by second order scattering with a novel rhodanine / Yu J [und weitere]. Fluorescence characteristics of novel chlorophenyl-arsenoxylphenylazo rhodanines and application in the determination of thallium (I) / Yu J [und weitere]. Molecular recognition of amino acids by hematoporphyrin and metallohematoporphyrin receptors / Zhang Y, Lei Y-C and Liu D-S. Determination of BSA by its enhancement effect on second order scattering of 3-(4'-methyl phenyl)-5-(4'-methyl-2'-sulfophenylazo) rhodanine / Zhu Y [und weitere] Separation and detection of amino acids with a novel capillary electrophoresis chemiluminescence system / Yin DG [und weitere]. A novel chemiluminescent immunoassay of total thyroxine using the acridinium ester 2',6'-dimethyl-4' -(N-succinimidyloxycarbonyl) phenyl-1O-methyl-acridinium-9-carboxylate methosulfate as label / Yin DG [und weitere]. Determination of ascorbic acid by a flow injection chemiluminescence method with a novel rhodanine / Yu J [und weitere]. Study of superweak luminescence in plants and application to salt tolerance in alfalfa / Zhou H, Yang Q and Liu Y. Development and optimization of a quantitative western blot and dot blot procedure for the determination of residual host cell proteins present in inactivated polio vaccine using a GZll based signal reagent / Zomer G [und weitere]. Development and optimization of a fast and sensitive ELISA for polio D-antigen using a GZll based signal reagent / Zomer G and Hamzink M -- pt. 5. Applied electroluminescence. Detection of Xanthomonas oryzae pv. Oryzicola by electrochemiluminescence polymerase chain reaction method / Wei J and Zhang L.A novel electrochemiluminescent sensor based on cationic polymer/chitosan for ultrasensitive detection of hydrogen peroxide / Wu X [und weitere]. Capillary electrophoresis - electrochemiluminescence detection of ciprofloxacin in biological fluids / Zhou X and Jia L -- pt. 6. Biomedical application of fluorescent protens. A novel multicolor fluorescent protein from the soft coral Scleronephthya gracillima Kuekenthal / Kato Y [und weitere]. Fluorescence from S[symbol]-level of complexes of tryptophan with europium (III) in water-ethanol solution / Osina IO, Ostahov Sand Kazakov V. Identification of developmental enhancers using targeted regional electroporation (TREP) of evolutionarily conserved regions / Pira CU [und weitere] -- pt. 7. Development and biomedical applications of quantum dots and other inorganic fluorescent materials