Biological Magnetic Resonance: Volume 19: Distance Measurements in Biological Systems by EPR (Biological Magnetic Resonance)

Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.

the Foundation For Understanding The Function And Dynamics Of Biological Systems Is Knowledge Of Their Structure. Many Experimental Methodologies Are Used For Determination Of Structure, Each With Special Utilities. The Volumes In This Series On Biological Magnetic Resonance Emphasize The Methods That Involve Magnetic Resonance. This Volume Seeks To Provide A Critical Evaluation Of Epr Methods For Determining The Distances Between Two Unpaired Electrons. The Editors Invited The Authors To Make This A Very Practical Book, With Specific Numerical Examples Of How Experimental Data Are Worked Up To Produce A Distance Estimate, And Realistic Assessments Of Uncertainties And Of The Range Of Applicability, Along With Examples Of The Power Of The Technique To Answer Biological Problems.

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some Of The Pioneers In The Field Explain The Use Of Nuclear Magnetic Resonance And Electron Spin Resonance Spectroscopy To Observe And Monitor The Physiology And Pathology Of Internal Organs And Tissues. Among The Topics Are Clinical Localization Techniques, Studying Brain Ischemia, Animal Studies, And Techniques Using Sodium, Fluoride, And Hydrogen. Annotation C. Book News, Inc., Portland, Or (booknews.com)

Front Matter....Pages i-xviii Distance Measurements by CW and Pulsed EPR....Pages 1-27 Relaxation Times of Organic Radicals and Transition Metal Ions....Pages 29-154 Structural Information from CW-EPR Spectra of Dipolar Coupled Nitroxide Spin Labels....Pages 155-184 Determination of Protein Folds and Conformational Dynamics Using Spin-Labeling EPR Spectroscopy....Pages 185-247 EPR Spectroscopic Ruler: the Method and its Applications....Pages 249-276 TOAC....Pages 277-307 Depth of Immersion of Paramagnetic Centers in Biological Systems....Pages 309-345 Determination of Distances Based on T 1 and T m Effects....Pages 347-381 Double-Quantum ESR and Distance Measurements....Pages 383-459 “2+1” Pulse Sequence as Apllied for Distance and Spatial Distribution Measurements of Paramagnetic Centers....Pages 461-491 Double Electron-Electron Resonance....Pages 493-512 Electron Paramagnetic Resonance Distance Measurements in Photosystems....Pages 513-567 Photo-Induced Radical Pairs Investigated Using Out-Of-Phase Electron Spin Echo....Pages 569-596 Back Matter....Pages 597-614 Although X-ray crystallography might seem to be the paradigm for structural studies, it is of little use for studying species in intact cells, for example.