Biochemistry and Biotechnology: Research and Development (Biochemistry Research Trends; Biotechnology in Agriculture, Industry and Medicine)

This book presents current research in the fields of biochemistry and biotechnology. Topics discussed include information about the production of thermostable DNA polymerase suitable for whole-blood polymerase chain reaction; isolation, purification and some properties of L-Lysine α-Oxidase from Trichodermа sp. 6; production of nitric oxide by endothelial cells infected with herpes simplex virus type 1; optimization of the process of cultivation of recombinant Escherichia coli TBI protein producing strain; development of HRP-functionalized carbon-coated iron nanoparticales using arenediazonium tosilates; experimental approach to the induction of nonculturable state of Lactococcus lactis and callus culture technology of spring soft wheat stress tolerant varieties. Biochemistry and Biotechnology: Research and Development 4 Contents 6 Preface 10 1 Production of Thermostable DNA Polymerase Suitable for Whole-Blood Polymerase Chain Reaction 16 Abstract 16 1. Introduction 16 2. Materials and Methods 17 3. Results and Discussion 18 Acknowledgments 20 References 20 2 Isolation, Purification and Some Properties of L-Lysine α-Oxidase from Trichodermа sp. 6 22 Abstract 22 Material and Methods 23 Results and Discussion 25 Conclusion 30 References 30 3 Production of Nitric Oxide by Endothelial Cells Infected with Herpes Simplex Virus Type 1, under the Influence of Interferon 32 Abstract 32 Introduction 33 Materials and Methods 33 Results and Discussion 34 References 38 4 Optimization of the Process of Cultivation of Recombinant Escherichia Coli TBI Protein Producing Strain 40 Abstract 40 Introduction 40 Materials and Methods 41 Results 42 Conclusion 47 Acknowledgments 48 References 48 5 Obtaining and Properties of Intracellular Aminoacylase Escherichia Coli 50 Abstract 50 Materials and Methods 51 References 53 6 Development of HRP-Functionalized Carbon-Coated Iron Nanoparticles Using Arenediazonium Tosylates 54 Abstract 54 Introduction 54 Materials and Methods 55 Results and Discussions 56 Conclusion 58 Acknowledgments 58 References 58 7 Experimental Approach to the Induction of Nonculturable State of Lactococcus Lactis 60 Abstract 60 Introduction 60 Materials and Methods 61 Results and Discussion 61 Conclusion 63 References 63 8 Callus Culture Technology of Spring Soft Wheat Stress Tolerant Varieties Selection 66 Abstract 66 Introduction 66 Materials and Methods 67 Results 67 Hormone Media Composition Optimization and Detection of Explants Parameters Suitable for Technology 67 Optimization of Levels of Selective Agents 70 Multiple Regeneration Phenomenon 71 Physiological Tolerance Tests of Regenerant Lines Created on Selective Media 73 Comparative Characterization of Salinity Tolerance 73 Acidity Tolerance Characterization 73 Conclusion 75 References 75 9 Structural Investigation of the Interaction between Bovine Serum Albumin and Acid Gelatin in Water 78 Abstract 78 Introduction 78 2. Experimental Section 80 2.1. Materials 80 2.2. Dynamic Light Scattering 81 2.3. Circular Dichroism Measurements 81 2.4. Fluorescence Measurements 81 2.5. Turbidimetry 81 2.6. High-Sensitivity DSC 82 3. Results 82 3.1. Hydrodynamic Radiuses and Zeta Potentials of BSA, Gelatin and their Mixtures 82 3.2. Effect of Gelatin on the Secondary Structure of BSA (CD Spectra) 84 3.3. Conformational Changes (Fluorescence Measurements) 85 Aggregation of BSA 86 3.4. Thermal Denaturation (DSC Measurements) 88 4. Discussion 89 4.1. Behaviour of BSA Molecules in Water 89 4.2. Behaviour of BSA Molecules in the Presence of Gelatin 90 Conclusion 92 References 92 10 The Effect of Nano- and Microparticles on Phase Separation and the Structure of Semidilute Biopolymer Mixtures 96 Abstract 96 1. Introduction 97 Materials and Methods 98 Results and Discussion 100 Conclusion 106 References 107 11 Adsorption Behaviour of 5-Methylresorcinol and Its Mixtures with Lysozyme at Air-Water Interface 110 Abstract 110 Introduction 110 Materials and Methods 112 Surface Tension Measurement 112 Results and Discussion 112 Adsorption of Methylresorcinol 112 Adsorption of Methylresorcinol in the Presence of Lysozyme 117 Conclusion 118 References 118 12 New Probiotic Product for Prevention and Treatment of Enteritis and Respiratory Diseases of Farm Stock 120 Abstract 120 Materials and Methods 121 Results and Discussion 122 References 126 13 Prebiotics Extraction from Valeriana officinalis L. Extraction Cake 128 Abstract 128 References 132 14 Variations in Physical-Chemical Properties of Block-Copolymer Chitosan with Methyl Acrylate during Biodegradation under the Action of Aspergillus Terreus 134 Abstract 134 1. Introduction 134 2. Materials and Methods 135 3. Results and their Discussion 139 Conclusion 142 Acknowledgments 143 References 143 15 The Destrction of the Compositions Based on Chitosan Copolymers with Acrylic Monomers by Micromycetes 146 Abstract 146 Introduction 146 Experimental Conditions 148 Results and Argument 151 References 156 16 Brassinosteroids as Possible Nanoregulators of Biological Systems 158 Abstract 158 1. Introduction 158 2. Brief of Previous Work 159 3. Results 160 Conclusion 160 References 161 17 The Possibility of Use of Extracts from Higher Hydrophytes and Geophytes of the Astrakhan Region for the Needs of Cosmetology 162 Abstract 162 1. Introduction 163 2. Purpose and Research Methods 163 3. Results 165 Conclusion 166 References 166 18 Antioxidantive Activity of Dry and Germinated Seeds of Agricultural Cultures 168 Abstract 168 Introduction 168 Method of Experiment 170 Results and Discussion 171 References 173 19 Filamentous Fungi of Waste Biomass of Higher Water Plants 174 Abstract 174 References 177 20 Purification of Kaolin of Different Grades with Application of Silicate Microorganisms 178 Abstract 178 References 181 21 Influence of Verbactin Preparation on Microbiological Activity of Irrigated Serozyon Soils 182 Abstract 182 Methods of Study 183 Results and Discussion 183 References 187 22 Features of Teaching of the Ecological Right at Saving Biodiversity of Sochi Black Sea Coast 188 23 Clonal Micropropagation Endemic of the Northern Caucasus Mentha Longifolia (L.) Huds 192 Abstract 192 References 194 24 Biotechnology for Landscape Construction in Russian Subtropics 196 References 198 25 Tasks for Training of Specialists for Biotechnology in the Educational Process in the Sochi Branch of People's Friendship University in the Area of Environmental Problems in the Region 202 Abstract 202 References 204 Index 206 1. Production of Thermostable DNA Polymerase Suitable for Whole-Blood Polymerase Chain Reaction (A. S. Korovashkina, S. V. Kvach, L. A. Eroshevskaya, A. I. Zinchenko) 2. Isolation, Purification and Some Properties of L-Lysine α-Oxidase from Trichodermа sp. 6 (K.V. Makrushin, A.G. Medentzev, A.Yu Arinbasarova,E.V. Lukasheva, T.T. Berezov) 3. Production of Nitric Oxide by Endothelial Cells Infected with Herpes Simplex Virus Type 1, under the Influence of Interferon (O.N. Scheglovitova, Z.V., Kuropteva, L.G. Nagler, N.N. Skliankona, N.A. Boldyreva, L.M. Bayder, O.L. Belaia) 4. Optimization of the Process of Cultivation of Recombinant Escherichia coli TBI Protein Producing Strain (N.I. Akulova, T.A. Kashperova, E.A. Volosnikova, L.R. Lebedev) 5. Obtaining and Properties of Intracellular Aminoacylase Escherichia coli (H.S. Yepremyan) 6. Development of HRP-Functionalized Carbon-Coated Iron Nanoparticales using Arenediazonium Tosilates (Alexandra G. Pershina1, Olga A. Lytkina, Pavel S. Postnikov, Marina E. Trusova, Dmitri Yu. Svitich, Viktor D. Filimonov, Alexey E. Sazonov) 7. Experimental Approach to the Induction of Nonculturable State of Lactococcus Lactis (Pakhomov Yu.D., Belus S.K., Blinkova L.P., Stoyanova L.G., Ustyugova E.A.) 8. Callus Culture Technology of Spring Soft Wheat Stress Tolerant Varieties Selection (V.Ju. Stupko, N.V. Zobova) 9. Structural Investigation of the Interaction between Bovine Serum Albumin and Acid Gelatin in Water (Y.A. Antonov, I.L. Zhuravleva, I.G. Plaschina) 10. The Effect of Nano- and Microparticles on Phase Separation and the Structure of Semidilute Biopolymer Mixtures (Y.A. Antonov) 11. Adsorption Behaviour of 5-Methylresorcinol and its Mixtures with Lysozyme at Air/Water Interface (E.I. Martirosova, I.G. Plashchina) 12. New Probiotic Product for Prevention and Treatment of Enteritis and Respiratory Diseases of Farm Stock (N.V. Sverchkova, M.S. Kolosovskaya, T.V. Romanovskaya, G.V. Zhuk, E.I.Kolomiets, P.A. Krasochko, Y.V. Lomako) 13. Prebiotics Extraction from Valeriana officinalis L. Extraction Cake (E.V. Ozhimkova, A.I. Sidorov, B.B. Tikhonov, E.I. Martirosova) 14. Variations in Physical-Chemical Properties of Block-Copolymer Chitosan with Methyl Acrylate During Biodegradation under the Action of Aspergillus Terreus (V. F. Uryash, I. N. Kalashnikov, L. A. Smirnova, N. Yu. Kokurina, A. E. Mochalov, V. F. Smirnov, O. N. Smirnova, and V. N. Larina) 15. The Destrction of the Compositions based on Chitosan Copolymers with Acrylic Monomers By Micromycetes (K.A. Zotov, V.F. Smirnov, L.A. Smirnova, A.E. Motchalova, D.V. Kryazhev, N.E. Tsverova) 16. Brassinosteroids as Possible Nanoregulators of Biological Systems (Mikhail A. Egorov) 17. The Possibility of Use of Extracts from Higher Hydrophytes and Geophytes of the Astrakhan Region for the Needs of Cosmetology (Oxana V. Astafieva, Olga V. Novichenko, Mikhail A. Egorov) 18. Antioxidantive Activity of Dry and Germinated Seeds of Agricultural Cultures (Z.G. Kozlova and N.D. Shaskolskaya) 19. Filamentous Fungi of Waste Biomass of Higher Water Plants (S.I. Kukanova, J.J. Tashpulatov, L.I. Zaynitdinova, T.S. Shulman, M.C. Bakhtiyorova, T.F. Aripov) 20. Purification of Kaolin of Different Grades with Application of Silicate Microorganisms (L.I. Zaynitdinova, S.I. Kukanova, A.A. Ismatov, I.V. Lobanova) 21. Influence of Verbactin Preparation on Microbiological Activity of Irrigated Serozyon Soils (G.D. Zolotilina, S.M. Khodjibaeva, Fedorova O.A., J.J. Tashpulatov) 22. Features of Teaching of the Ecological Right at Saving Biodiversity of Sochi Black Sea Coast (A.A. Rybalko) 23. Clonal mMicropropagation Endemic of the Northern Caucasus Mentha Longifolia (L.) Huds (A.E. Rybalko A.E., S.L. Gubaz) 24. Biotechnology for Landscape Construction in Russian Subtropics (A.E. Rybalko, V.P. Tkachenko, A.A. Rybalko AA, L.S.Samarina) 25. Tasks for Training of Specialists for Biotechnology in the Educational Process in the Sochi Branch of People's Friendship University in the Area of Environmental Problems in the Region (N.I. Kozlowa, A.B. Rybalko, K.P. Skipina, L.G. Kharuta, A.A. Kozlowa) Index Production of thermostable DNA polymerase suitable for whole-blood polymerase chain reaction / A.S. Korovashkina ... [et al.] Isolation, purification, and some properties of L-lysine a-oxidase from trichoderm? sp. 6 / K.V. Makrushin ... [et al.] Production of nitric oxide by endothelial cells infected with herpes simplex virus type 1, under the influence of interferon / O.N. Scheglovitova ... [et al.] Optimization of the process of cultivation of recombinant Escherichia coli TBI protein producing strain / N.I. Akulova ... [et al.] Obtaining and properties of intracellular aminoacylase Escherichia coli / H.S. Yepremyan Development of HRP-functionalized carbon-coated iron nanoparticales using arenediazonium tosilates / Alexandra G. Pershinal ... [et al.] Experimental approach to the induction of nonculturable state of lactococcus lactis / Pakhomov Yu.D. ... [et al.] Callus culture technology of spring soft wheat stress tolerant varieties selection / V.Ju. Stupko, N.V. Zobova Structural investigation of the interaction between bovine serum albumin and acid gelatin in water / Y.A. Antonov, I.L. Zhuravleva, I.G. Plaschina The effect of nano- and microparticles on phase separation and the structure of semidilute biopolymer mixtures / Y.A. Antonov Adsorption behaviour of 5-methylresorcinol and its mixtures with lysozyme at air/water interface / E.I. Martirosova, I.G. Plashchina New probiotic product for prevention and treatment of enteritis and respiratory diseases of farm stock / N.V. Sverchkova ... [et al.] Prebiotics extraction from Valeriana officinalis L. extraction cake / E.V. Ozhimkova ... [et al.] Variations in physical-chemical properties of block-copolymer chitosan with methyl acrylate during biodegradation under the action of aspergillus terreus / V.F. Uryash ... [et al.] The destrction of the compositions based on chitosan copolymers with acrylic monomers by micromycetes / K.A. Zotov ... [et al.] Brassinosteroids as possible nanoregulators of biological systems / Mikhail A. Egorov The possibility of use of extracts from higher hydrophytes and geophytes of the Astrakhan region for the needs of cosmetology / Oxana V. Astafieva, Olga V. Novichenko, Mikhail A. Egorov Antioxidantive activity of dry and germinated seeds of agricultural cultures / Z.G. Kozlova and N.D. Shaskolskaya Filamentous fungi of waste biomass of higher water plants / S.I. Kukanova ... [et al.] Purification of kaolin of different grades with application of silicate microorganisms / L.I. Zaynitdinova ... [et al.] Influence of verbactin preparation on microbiological activity of irrigated serozyon soils / G.D. Zolotilina ... [et al.] Features of teaching of the ecological right at saving biodiversity of Sochi Black Sea coast / A.A. Rybalko Clonal mmicropropagation endemic of the northern caucasus mentha longifolia (L.) huds / A.E. Rybalko A.E., S.L. Gubaz Biotechnology for landscape construction in Russian subtropics / A.E. Rybalko ... [et al.] Tasks for training of specialists for biotechnology in the educational process in the Sochi branch of People's Friendship University in the area of environmental problems in the region / N.I. Kozlowa ... [et al.]. This book presents current research in the fields of biochemistry and biotechnology. Topics discussed include information about the production of thermostable DNA polymerase suitable for whole-blood polymerase chain reaction; isolation, purification and some properties of L-Lysine Æ'¿-Oxidase from Trichoderm„p sp. 6; production of nitric oxide by endothelial cells infected with herpes simplex virus type 1; optimization of the process of cultivation of recombinant Escherichia coli TBI protein producing strain; development of HRP-functionalized carbon-coated iron nanoparticales using arenediazonium tosilates; experimental approach to the induction of nonculturable state of Lactococcus lactis and callus culture technology of spring soft wheat stress tolerant varieties.