Biochemical Engineering

Preface Chapter 1 Introduction Importance of bioprocessing and scale-up in new biotechnology. Chapter 2 Enzyme Kinetics Simple enzyme kinetics, enzyme reactor, inhibition, other influences, and experiments Chapter 3 Immobilized Enzymes Immobilization techniques and effect of mass transfer resistance Chapter 4 Industrial Applications of Enzymes Carbohydrates, starch conversion, cellulose conversion, and experiments Chapter 5 Cell Cultivations Microbial, animal, and plant cell cultivations, cell growth measurement, cell immobilization, and experiments Chapter 6 Cell Kinetics and Fermenter Design Growth cycle, cell kinetics, batch or plug-flow stirred-tank fermenter, continuous stirred-tank fermenter (CSTF), multiple fermenters in series, CSTF with cell recycling, alternative fermenters, and structured kinetic models Chapter 7 Genetic Engineering DNA and RNA, cloning of genes, stability of recombinant cells, and genetic engineering of plant cells Chapter 8 Sterilization Sterilization methods, thermal death kinetics, design criterion, batch and continuous sterilization, and air sterilization Chapter 9 Agitation and Aeration Basic mass-transfer concepts, correlations form mass-transfer coefficient, measurement of interfacial area, correlations for interfacial area, gas hold-up, power consumption, oxygen absorption rate, scale-up, and shear sensitive mixing Chapter 10 Downstream Processing Solid-liquid separation, cell rupture, recovery, and purification