Autophagy in Disease and Clinical Applications, Part C (Volume 453) (Methods in Enzymology, Volume 453)

The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth. * Provides an overview of autophagy in regards to humans, specifically regarding disease connections and clinical samples. * Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture *Presents reliable methods that, in this relatively new field, allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes Content: Series Editors Page ii Copyright Page Page iv Contributors to Volume 453 Pages xiii-xix Preface Pages xxi-xxii Daniel J. Klionsky Volumes in Series Pages xxiii-xlix Chapter 1 Initiation of Autophagy by Photodynamic Therapy Review Article Pages 1-16 David Kessel, Nancy L. Oleinick Chapter 2 Autophagic Cell Death Review Article Pages 17-31 Michael J. Lenardo, Christina K. McPhee, Li Yu Chapter 3 Autophagic Neuron Death Review Article Pages 33-51 Yasuo Uchiyama, Masato Koike, Masahiro Shibata, Mitsuho Sasaki Chapter 4 Assessing Metabolic Stress and Autophagy Status in Epithelial Tumors Review Article Pages 53-81 Robin Mathew, Vassiliki Karantza‐Wadsworth, Eileen White Chapter 5 Autophagic Clearance of Aggregate‐Prone Proteins Associated with Neurodegeneration Review Article Pages 83-110 Sovan Sarkar, Brinda Ravikumar, David C. Rubinsztein Chapter 6 Monitoring Autophagy in Alzheimer's Disease and Related Neurodegenerative Diseases Review Article Pages 111-144 Dun‐Sheng Yang, Ju‐Hyun Lee, Ralph A. Nixon Chapter 7 Live‐Cell Imaging of Autophagy Induction and Autophagosome‐Lysosome Fusion in Primary Cultured Neurons Review Article Pages 145-158 Mona Bains, Kim A. Heidenreich Chapter 8 Using Genetic Mouse Models to Study the Biology and Pathology of Autophagy in the Central Nervous System Review Article Pages 159-180 Zhenyu Yue, Gay R. Holstein, Brian T. Chait, Qing Jun Wang Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy‐Deficient Mice Review Article Pages 181-196 Satoshi Waguri, Masaaki Komatsu Chapter 10 Analyzing Autophagy in Clinical Tissues of Lung and Vascular Diseases Review Article Pages 197-216 Hong Pyo Kim, Zhi‐Hua Chen, Augustine M.K. Choi, Stefan W. Ryter Chapter 11 Autophagy in Neurite Injury and Neurodegeneration: In Vitro and In Vivo Models Review Article Pages 217-249 Charleen T. Chu, Edward D. Plowey, Ruben K. Dagda, Robert W. Hickey, Salvatore J. Cherra III, Robert S.B. Clark Chapter 12 Monitoring the Autophagy Pathway in Cancer Review Article Pages 251-271 Frank C. Dorsey, Meredith A. Steeves, Stephanie M. Prater, Thomas SchrГ¶ter, John L. Cleveland Chapter 13 Autophagy Pathways in Glioblastoma Review Article Pages 273-286 Hong Jiang, Erin J. White, Charles Conrad, Candelaria Gomez-Manzano, Juan Fueyo Chapter 14 Autophagy in Lung Cancer Review Article Pages 287-304 Jerry J. Jaboin, Misun Hwang, Bo Lu Chapter 15 Signal-Dependent Control of Autophagy-Related Gene Expression Review Article Pages 305-324 Fulvio Chiacchiera, Cristiano Simone Chapter 16 Novel Methods for Measuring Cardiac Autophagy In Vivo Review Article Pages 325-342 Cynthia N. Perry, Shiori Kyoi, Nirmala Hariharan, Hiromitsu Takagi, Junichi Sadoshima, Roberta A. Gottlieb Chapter 17 Autophagy in Load‐Induced Heart Disease Review Article Pages 343-363 Hongxin Zhu, Beverly A. Rothermel, Joseph A. Hill Chapter 18 Evaluation of Cell Death Markers in Severe Calcified Aortic Valves Review Article Pages 365-378 Wilhelm Mistiaen, Michiel Knaapen Chapter 19 Monitoring Autophagy in Muscle Diseases Review Article Pages 379-396 May Christine V. Malicdan, Satoru Noguchi, Ichizo Nishino Chapter 20 Analyzing Macroautophagy in Hepatocytes and the Liver Review Article Pages 397-416 Wen-Xing Ding, Xiao-Ming Yin Chapter 21 Monitoring Autophagy in Lysosomal Storage Disorders Review Article Pages 417-449 Nina Raben, Lauren Shea, Victoria Hill, Paul Plotz Author Index Pages 451-470 Subject Index Pages 471-481 The third and final installment of Daniel J. Klionsky's new three-volume treatment of autophagy, this volume focuses on monitoring autophagy with regard to disease connections, and presents methods that can be used to analyze autophagy in clinical samples. Edited by one of the leading authorities in the field, this volume and its companion volumes, Autophagy: Lower Eukaryotes and Autophagy in Mammalian Systems, provide a comprehensive overview of the techniques involved in studying autophagy in eukaryotes and simple animal systems, mammalian cells and non-human animals, and humans. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house cleaning" in the cell is called autophagy - literally self-eating. In the future, research in this field will help to design clinical approaches that can turn on autophagy and halt tumor growth. Provides an overview of autophagy in regards to humans, specifically regarding disease connections and clinical samples. Includes methods to evaluate the role of autophagy in the drug-induced cell death of cancer cells in culture, helping researchers design clinical approaches that can halt tumor growth Presents reliable methods that, in this relatively new field, allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal 'house-cleaning' in the cell is called autophagy - literally self-eating. This text looks at the monitoring of autophagy