Atlas of Human Pluripotent Stem Cells in Culture

This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. __Atlas of Human Pluripotent Stem Cells in Culture__ is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing. This Lavishly-illustrated, Authoritative Atlas Explores The Intricate Art Of Culturing Human Pluripotent Stem Cells. Twelve Chapters – Containing More Than 280 Color Illustrations – Cover A Variety Of Topics In Pluripotent Stem Cell Culturing Including Mouse And Human Fibroblasts, Human Embryonic Stem Cells And Induced Pluripotent Stem Cells, Characteristic Staining Patterns, And Abnormal Cultures, Among Others. Atlas Of Human Pluripotent Stem Cells In Culture Is A Comprehensive Collection Of Illustrated Techniques Complemented By Informative And Educational Captions Examining What Good Quality Cells Look Like And How They Behave In Various Environments. Examples Of Perfect Cultures Are Compared Side-by-side To Less-than-perfect And Unacceptable Examples Of Human Embryonic And Induced Pluripotent Stem Cell Colonies. This Detailed And Thorough Atlas Is An Invaluable Resource For Researchers, Teachers, And Students Who Are Interested In Or Working With Stem Cell Culturing. 1 Before We Begin: An Introduction To The Culture Of Pluripotent Cells -- 2 Mouse And Human Fibroblasts -- 3 Inactivated Mouse And Human Fibroblasts -- 4 Human Embryonic Stem Cells -- 5 Human Induced Pluripotent Stem Cells On Feeders -- 6 Pluripotent Cell Lines Grown On Different Substrates And Surfaces -- 7 Passaging Pluripotent Stem Cells -- 8 Characteristic Staining Patterns Of Undifferentiated And Differentiated Pluripotent Stem Cells -- 9 Derivation Of Induced Pluripotent Stem Cells -- 10 Culture Adaptation And Abnormal Cultures -- 11 Infection -- 12 Miscellaneous Cell Types And Cell Lines Of Interest. By Lyn Healy, Ludmila Ruban. Front Matter....Pages i-xv Before We Begin: An Introduction to the Culture of Pluripotent Cells....Pages 1-2 Mouse and Human Fibroblasts....Pages 3-18 Inactivated Mouse and Human Fibroblasts....Pages 19-34 Human Embryonic Stem Cells....Pages 35-74 Human Induced Pluripotent Stem Cells on Feeders....Pages 75-100 Pluripotent Cell Lines Grown on Different Substrates and Surfaces....Pages 101-120 Passaging Pluripotent Stem Cells....Pages 121-129 Characteristic Staining Patterns of Undifferentiated and Differentiated Pluripotent Stem Cells....Pages 131-148 Derivation of Induced Pluripotent Stem Cells....Pages 149-165 Culture Adaptation and Abnormal Cultures....Pages 167-175 Infection....Pages 177-185 Miscellaneous Cell Types and Cell Lines of Interest....Pages 187-198 Back Matter....Pages 199-206