Assessment of the Use of Single Cytochrome P450 Enzymes in Drug Research (Ernst Schering Foundation Symposium Proceedings, 13)

Metabolic Research And Especially That Which Focuses On The Monooxygenase Cytochrome P450 System Has Gone Through Dramatic Progressive Changes Within The Last Decade. Availability Of Highly Sophisticated Analytical Techniques Has Contributed To Increased Molecular Understanding Of Biotransformation Pathways. Nowadays Designer Drugs Are Computer Composable Focussing On Metabolic Stability. The Rapid Development Of Recombinant Enzymes Has, In Many Cases, Replaced The Intensive Work On P450 Isolation From Naturally Occurring Sources. Recombinant P450s Can Be Expressed In Cell Lines And Thus Made Available For Reproducible And Constant Use Addressig A Large Number Of Scientific Questions. This Book Describes The Present Position Of Such Research. Front Matter....Pages I-XII Xenobiotic Metabolism: From Intact Biosystem to Single Enzymes....Pages 1-19 Regulation of Xenobiotic-Metabolizing Cytochromes P450....Pages 21-42 Cytochrome P450 in Human Drug Metabolism: How Much Is Predictable?....Pages 43-56 Metabolic Activation of Anticancer Oxazaphosphorines by Cytochrome P450s: Development of a Model for Cancer Gene Therapy....Pages 57-80 The Use of Bacteria for Cytochrome P450 Expression....Pages 81-95 Expression of Cytochromes P450 in Yeast: Practical Aspects....Pages 97-110 Human Cells as an Expression System for Cytochromes P450....Pages 111-134 Metabolic Reactions and Recombinant Isoenzymes of Cytochrome P450: Information Generated and Value for Pharmaceutical Development....Pages 135-160 The Importance of Cytochrome P450 3A Enzymes in Drug Metabolism....Pages 161-186 Strategies for Verifying the Involvement of Specific Cytochrome P450 Enzymes in Xenobiotic Metabolism....Pages 187-194 Regulation of Cytochromes P450 by Substrate Interactions....Pages 195-211 Strategies for the Use of Single Cytochrome P450 Enzymes in Drug Research and Future Prospects....Pages 213-223 Back Matter....Pages 225-227