Arthropod Cell Cultures And Their Application To The Study Of Viruses (current Topics In Microbiology And Immunology)

To lose is human, to win is fortune, but to try is our destiny. EARL C. SUITOR, JR. The idea of a Symposium on "Arthropod Cell Cultures" started in July of 1969 shortly after the untimely death of our colleague, EARL C. SUITOR, JR. , at the age of 38. At first we thought an afternoon or evening session would be sufficient, but we were soon convinced that the scope of the Symposium should be greatly enlarged. Interest in this topic was increasing at an astonishing rate. Since EARL SUITOR had made a distinct imprint in this new field, many scientists who knew him well or just casually wished to honor him in this manner. EARL SUITOR was born and raised in New England and received a B. S. degree in bacteriology from the University of Massachusetts in 1952. As a spirited young man, he enlisted in the U. S. Navy to see the world. Instead, he was assigned for most of his four-year "hitch" to the Naval Medical Research Institute. I met him there in 1954, an enthusiastic and imaginative young fellow with many interests, an avid reader of the classics, an occasional writer of poetry, who blended his interest in scientific literature with that of Science Fiction. In 1956, EARL left the Navy to attend George Washington University, where he earned an M. S. degree in 1958 and a Ph. D. degree in 1963. Front Matter....Pages N3-XX Cultivation of Dipteran Cells in Vitro ....Pages 1-12 Culture of Cells of the Flesh Fly, Sarcophaga bullata ....Pages 12-19 Establishment and Characterization of Two New Cell Lines (CP - 1268 and CP -169) from the Codling Moth, Carpocapsa pomonella (with a Review of Culture of Cells and Tissues from Lepidoptera)....Pages 19-28 A Cell Line Isolated from Hemocytes of Samia cynthia Pupae....Pages 28-32 Present Status of Tick Tissue Culture....Pages 32-41 Introduction....Pages 42-42 Morphological and Cultural Characteristics of Culiseta inornata and Aedes vexans Mosquito Cell Lines....Pages 42-47 Antigenic Relationships of Mosquito Cell Lines as Determined by Immunodiffusion Techniques....Pages 47-51 Characterization and Identification of Insect Cell Cultures....Pages 51-61 Cytogenetic Studies on Cells in Culture from the Class Insecta....Pages 61-69 Introduction....Pages 70-71 Drosophila Neuron Differentiation in Vitro....Pages 71-75 Cultivation of Insect Endocrine Glands in Vitro ....Pages 75-85 Production of Human-Mosquito Somatic Cell Hybrids and their Response to Virus Infection....Pages 85-92 The Uptake of Free and Serum-Bound Sterols by Insect Cells in Vitro ....Pages 92-97 Comparative Lipid Analysis of Aedes aegypti and Monkey Kidney Cells (MK-2) Cultivated in Vitro ....Pages 97-102 Studies Attempting to Elucidate the in Vivo Function(s) of Grace’s Antheraea eucalypti Cell Strains....Pages 102-107 The Effects of Insecticides on Insect Cells Grown in Vitro ....Pages 108-112 Arthropod Tissue Culture in the Study of Arboviruses and Rickettsiae: A Review....Pages 113-126 Growth of Arboviruses in Aedes albopictus and A. aegypti Cell Lines....Pages 127-133 Multiplication of Chikungunya and O’nyong-nyong Viruses in Singh’s Aedes Cell Lines....Pages 133-137 Growth Studies of California Encephalitis Virus in Two Aedes Mosquito Cell Line Cultures....Pages 138-139 Preliminary Observations on Growth of Arboviruses in a Newly Established Line of Mosquito Cell ( Culex quinquefasciatus Say )....Pages 140-148 Attempts to Grow Tacaribe and Junin Viruses in Grace’s Continuous Line of Moth Cells....Pages 149-150 A Comparative Study of the Viral Susceptibility of Monolayer and Suspended Mosquito Cell Lines....Pages 150-154 Attenuation of Semliki Forest (SF) Virus in Continuously Cultured Aedes aegypti Mosquito Cells ( Peleg ) as a Step in Production of Vaccines....Pages 155-161 Cultivation of Oncogenic Viruses in Mosquito Cells in Vitro ....Pages 161-164 RNA of Uninfected and Sindbis Virus-Infected Aedes albopictus Cells....Pages 164-169 Introduction....Pages 170-173 In Vitro Cultivation....Pages 173-186 Application to the Study of Plant Pathogens....Pages 186-195 Cultivation of Gypsy Moth Hemocytes....Pages 196-200 Detection of Viral Antigens of the Densonucleosis Virus by Antibodies Labeled with Peroxidase Enzyme....Pages 200-204 Growth of a Microsporidian Parasite in Cultured Cells of Tent Caterpillars ( Malacosoma )....Pages 204-208 Drosophila Cells in Vitro : Behavior and Utilization for Genetic Purpose....Pages 209-219 Established Diploid Cell Lines of Drosophila melanogaster as Potential Material for the Study of Genetics of Somatic Cells....Pages 220-227 Arbovirus Multiplication in an Established Diploid Cell Line of Drosophila melanogaster ....Pages 227-230 Sigma Virus Multiplication in Drosophila Cell Lines of Different Genotypes....Pages 230-233 The SR Condition in Drosophila bifasciata : In Vivo and in Vitro Growth....Pages 233-240 Some Speculations on the Possible Role of Arthropods in the Evolution of Arboviruses....Pages 241-245 Future Possibilities of Arthropod Cell Culture in Parasitology and in Developmental Biology....Pages 245-248 Back Matter....Pages 249-288