"Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine"

This book is not intended to be a basic text in infrared spectroscopy. Many such books exist and I have referred to them in the text. Rather, I have tried to find applications that would be interesting to a variety of people: advanced undergraduate chemistry students, graduate students and research workers in several disciplines, spectros­ copists, and physicians active in research or in the practice of medicine. With this aim in mind there was no intent to have exhaustive coverage of the literature. I should like to acknowledge my use of several books and reviews, which were invaluable in my search for material: G. H. Beaven, E. A. Johnson, H. A. Willis and R. G. 1. Miller, Molecular Spec­ troscopy, Heywood and Company, Ltd., London, 1961. J. A. Schell man and Charlotte Schellman, 'The Conformation of Polypeptide Chains in Proteins," in The Proteins, Vol. II, 2nd Ed. (H. Neurath, ed.), Academic Press, New York, 1964. R. T. O'Connor, "Application of Infrared Spectrophotometry to Fatty Acid Derivatives," J. Am. Oil Chemists' Soc. 33, 1 (1956). F. L. Kauffman, "Infrared Spectroscopy of Fats and Oils," J. Am. Oil Chemists' Soc. 41,4 (1964). W. J. Potts, Jr., Chemical Infrared Spectroscopy, Vol. I, Techniques, Wiley, New York, 1963. R. S. Tipson, Infrared Spectroscopy of Carbohydrates, National Bureau of Standards Monograph llO, Washington, D.C., 1968. C. N. R. Rao, Chemical Applications of Infrared Spectroscopy, Academic Press, New York, 1963. Front Matter....Pages i-xiv Introduction and Brief Theory....Pages 1-24 Near-Infrared Spectroscopy....Pages 25-40 Sampling Methods....Pages 41-79 Quantitative Analysis....Pages 80-83 Hydrogen Bonding....Pages 84-99 Carbohydrates....Pages 100-141 Lipids....Pages 142-164 Amides and Amines....Pages 165-172 Amino Acids, Related Compounds, and Peptides....Pages 173-187 Proteins and Polypeptides....Pages 188-231 Hydrogen-Deuterium Exchange....Pages 232-270 Nucleic Acids and Related Compounds....Pages 271-314 Steroids....Pages 315-349 Porphyrins and Related Compounds....Pages 350-363 Enzymology....Pages 364-389 Drugs, Pharmaceuticals, and Pharmacological Applications....Pages 390-417 Microbiology....Pages 418-439 Applications in Medicine and Related Fields....Pages 440-467 Special Topics of Interest in Biochemistry and Related Fields....Pages 468-535 Information Sources Concerning Infrared Spectroscopy and Spectra....Pages 536-543 Back Matter....Pages 544-601 1. Introduction and Brief Theory.- Theory (1).- Wave Number and Wavelength Units (2).- The Double-Beam Recording Spectrophotometer (2).- The Infrared Spectrum (4).- Calibration of Wave Number and Wavelength (5).- Examples of the Interpretation of Spectra (7).- Spurious bands (20).- References (24).- 2. Near-Infrared Spectroscopy.- (25).- Applications (26).- References (39).- 3. Sampling Methods.- Solids (41).- Solution Techniques (46).- Calibration of Infrared Absorption Cells (56).- Cells for Gases (57).- Micromethods (58).- Low-Temperature Work (61).- Attenuated Total Reflection (70).- The U