Applications of Hplc in Biochemistry (Laboratory Techniques in Biochemistry and Molecular Biology, Vol 17)

This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice. Content: Advisory board Page ii Edited by Page iii Copyright page Page iv Acknowledgements Page vi Chapter 1 The Origins and development of liquid chromatography Pages 1-7 Chapter 2 The theory of HPLC Pages 8-22 Chapter 3 Instrumentation Pages 23-42 Chapter 4 High Performance Ion-Exchange Chromatography Pages 43-55 Chapter 5 High performance size exclusion chromatography Pages 56-64 Chapter 6 High performance normal phase chromatography Pages 65-72 Chapter 7 High performance reversed phase chromatography Pages 73-89 Chapter 8 High performance reverse phase ion-pair chromatography Pages 90-101 Chapter 9 High performance affinity chromatography Pages 102-114 Chapter 10 Practical aspects Pages 115-142 Chapter 11.1 Nucleosides and nucleotides Pages 144-168 Chapter 11.2 Proteins, peptides and amino acids Pages 169-192 Chapter 11.3 Lipids Pages 193-212 Chapter 11.4 Carbohydrates Pages 213-229 Chapter 11.5 Prostaglandins, leukotrienes and hydroxyeicosatetraenoic acids Pages 230-243 Chapter 11.6 Steroids Pages 244-259 Chapter 11.7 Biogenic amines Pages 260-270 Chapter 11.8 Vitamins Pages 271-294 Chapter 11.9 Antibiotics Pages 295-305 Appendix Pages 306-311 References Pages 312-331 Subject index Pages 332-338