Antibody production in man : in vitro synthesis and clinical implications : proceedings of the Catharijne Conference on Immunology held in Utrecht, Holland on March 15-18, 1978

Antibody Production in Man: In Vitro Synthesis and Clinical Implications is composed of the proceedings of the Catharijne Conference on immunology held in Utrecht, Holland on March 15-18, 1978. The conference focuses on the in vitro induction and regulation of immunoglobulin and antibody synthesis by human lymphocytes. It aims to present, analyze, exchange, and criticize data among the different investigators and to attempt to resolve apparent inconsistencies and contradictions. The book first discusses the mitogen- and antigen-induced B-cell activation. It then explains the subpopulations of lymphocytes involved in the regulation of in vitro B-cell function and the clinical implications in the study of in vitro B-cell function. This book reflects the stimulation as well as the frustration during the workshop. It will serve as a small force and focus of direction in this most interesting, complex, and relevant area of human immunobiology. Front Cover......Page 1 Antibody Production in Man: In Vitro Synthesis and Clinical Implications......Page 4 Copyright Page......Page 5 Table of Contents......Page 6 CONTRIBUTORS......Page 10 PREFACE......Page 14 CHAPTER 1. CONSIDERATION OF ANTIBODY DEFICIENCY DISEASES IN RELATION TO NORMAL B-CELL DEVELOPMENT......Page 16 I. NORMAL DEVELOPMENT OF B CELLS......Page 17 REFERENCES......Page 28 I. INTRODUCTION......Page 32 II. MATERIALS AND METHODS......Page 33 III. RESULTS ......Page 37 IV. DISCUSSION......Page 43 REFERENCES......Page 47 I. INTRODUCTION......Page 50 II. OPTIMAL CONDITIONS FOR THE INDUCTION OF POLYCLONAL ANTIBODY RESPONSE BY LPS......Page 52 III. LOCALIZATION OF B CELLS RESPONDING TO DIFFERENT BACTERIAL STRAINS WITH ANTIBODY FORMATION......Page 55 IV. PBA PROPERTIES OF DIFFERENT BACTERIAL PREPARATIONS......Page 57 V. AGE-DEPENDENT RESPONSE TO DIFFERENT BACTERIA IN ADENOID LYMPHOCYTES......Page 58 VI. POLYCLONAL B CELL RESPONSE TO PPD IN CHILDREN WITH BCG INFECTION......Page 59 VII. SUMMARY......Page 61 REFERENCES......Page 62 I. INTRODUCTION......Page 64 II. METHODS......Page 65 III. RESULTS......Page 66 IV. DISCUSSION......Page 73 REFERENCES......Page 75 PART I: GENERAL DISCUSSION MITOGEN INDUCED B CELL ACTIVATION. I.......Page 76 I. INTRODUCTION......Page 84 II. MATERIALS AND METHODS......Page 85 III. RESULTS ......Page 89 IV. DISCUSSION......Page 95 V. SUMMARY......Page 97 REFERENCES......Page 98 I. INTRODUCTION......Page 100 II. MATERIALS AND METHODS......Page 101 Ill. RESULTS......Page 103 IV. DISCUSSION......Page 109 REFERENCES......Page 112 I. INTRODUCTION......Page 116 II. MATERIAL AND METHODS......Page 117 III. RESULTS......Page 120 REFERENCES......Page 127 PART II: GENERAL DISCUSSION MITOGEN-INDUCED B-CELL ACTIVATION. II.......Page 130 I. INTRODUCTION......Page 136 II. MATERIALS AND METHODS......Page 137 III. RESULTS AND DICUSSION......Page 138 REFERENCES......Page 152 I. INTRODUCTION......Page 156 II. MATERIALS AND METHODS......Page 157 III. RESULTS ......Page 161 IV. DISCUSSION......Page 165 V. SUMMARY......Page 170 REFERENCES......Page 171 I. INTRODUCTION......Page 174 III. RESULTS......Page 175 IV. DISCUSSION......Page 178 REFERENCES......Page 180 PART III: GENERAL DISCUSSION ANTIGEN INDUCED B CELL ACTIVATION. I.......Page 182 CHAPTER 11. T - B COLLABORATION IN THE IN VITRO AB RESPONSE TO DIPHTHERIA TOXOID; EVIDENCE FAVOURING A ROLE FOR B CELLS IN THE GENERATION OF T CELL HELP......Page 188 REFERENCES......Page 197 I. INTRODUCTION......Page 200 III. EFFECT OF EPSTEIN-BARR VIRUS (EBV)......Page 201 IV. EFFECT OF MODIFIED CULTURE CONDITIONS......Page 204 REFERENCES......Page 206 CHAPTER 13. HUMAN ANTIGEN SPECIFIC HELPER T CELL FACTORS IN B CELL RESPONSES......Page 208 II. MATERIALS AND METHODS......Page 209 Ill. RESULTS......Page 213 IV. DISCUSSION......Page 217 REFERENCES......Page 220 PART IV: GENERAL DISCUSSION ANTIGEN INDUCED B CELL ACTIVATION. II.......Page 224 I. INTRODUCTION......Page 230 II. MATERIALS AND METHODS......Page 231 III. RESULTS AND DISCUSSION......Page 232 REFERENCES......Page 235 I. INTRODUCTION......Page 238 II. MATERIALS AND METHODS......Page 239 III. RESULTS AND DISCUSSION......Page 240 REFERENCES......Page 245 I. INTRODUCTION......Page 246 II. MATERIALS AND METHODS......Page 247 III. RESULTS......Page 250 IV. DISCUSSION......Page 259 ACKNOWLEDGEMENTS......Page 265 REFERENCES......Page 266 PART V: GENERAL DISCUSSION SUBPOPULATIONS OF LYMPHOCYTES INVOLVED IN THE IN VITRO REGULATION OF B CELL FUNCTION. I.......Page 268 CHAPTER 17. GENERATION OF PLAQUE-FORMING CELLS: ALLOGENEIC EFFECT AND SIGNIFICANCE OF MONOCYTE REMOVAL......Page 274 I. MATERIALS AND METHODS......Page 275 II. RESULTS......Page 277 III. DISCUSSION......Page 282 ACKNOWLEDGMENTS......Page 285 REFERENCES......Page 286 I. INTRODUCTION......Page 288 II. POLYCLONAL INDUCTION OF AN IN VITRO PFC RESPONSE......Page 289 III. CHARACTERIZATION OF HUMAN PERIPHERAL BLOOD SUPPRESSOR CELLS......Page 294 IV. DISCUSSION......Page 301 REFERENCES......Page 303 I. INTRODUCTION......Page 306 II. METHODS......Page 307 III. RESULTS......Page 309 REFERENCES......Page 316 PART VI: GENERAL DISCUSSION SUBPOPULATIONS OF LYMPHOCYTES INVOLVED IN THE IN VITRO REGULATION OF B CELL-FUNCTION. II.......Page 318 I. INTRODUCTION......Page 324 II. PRIMARY HUMORAL IMMUNODEFICIENCY......Page 325 III. SEVERE COMBINED IMMUNODEFICIENCY DISEASE......Page 335 REFERENCES......Page 338 CHAPTER 21. IMMUNOGLOBULIN SECRETING CELLS IN THE CIRCULATING BLOOD AND IN MITOGEN STIMULATED CULTURES FROM PATIENTS WITH IMMUNODEFICIENCY DISEASES......Page 340 REFERENCES......Page 346 CHAPTER 22. POLYCLONAL B-CELL ACTIVATORS IN THE ANALYSIS OF IMMUNODEFICIENCY STATES......Page 348 I. SPECIFIC UNRESPONSIVENESS DUE TO LACK OF V GENE EXPRESSION......Page 349 III. UNRESPONSIVENESS DUE TO LACK OF A PARTICULAR PBA RECEPTOR......Page 351 IV. UNRESPONSIVENESS DUE TO LACK OF A SUBSTANTIAL SUBPOPULATION OF B CELLS......Page 352 V. UNRESPONSIVENESS DUE TO INDUCTION OF IMMUNOLOGICAL TOLERANCE......Page 353 VI. GENERALIZED IMMUNODEFICIENCIES......Page 355 REFERENCES......Page 357 PART VII: GENERAL DISCUSSION CLINICAL IMPLICATIONS IN THE STUDY OF IN VITRO B CELL FUNCTION. I.......Page 360 I. INTRODUCTION......Page 366 II. MATERIAL AND METHODS......Page 367 III. RESULTS......Page 369 REFERENCES......Page 380 CHAPTER 24. THE CELLULAR DEFECT IN IgA DEFICIENCY: POSSIBLE LINKS TO HYPOGAMMAGLOBULINAEMIA......Page 382 REFERENCES......Page 391 I. INTRODUCTION......Page 394 II. MATERIALS AND METHODS......Page 395 III. RESULTS......Page 396 IV. DISCUSSION......Page 400 REFERENCES......Page 401 PART VIII: GENERAL DISCUSSION CLINICAL IMPLICATIONS IN THE STUDY OF IN VITRO B CELL FUNCTION. II.......Page 404 SUMMARY OF WORKSHOP: HUMAN B CELL FUNCTION: RECENT ADVANCES, UNANSWERED QUESTIONS, AND FUTURE DIRECTIONS......Page 408 INDEX......Page 412