Anion Carriers of Mitochondrial Membranes

In order to provide a functional organisation of the various biochemical processes, the intracellular environment is subdivided into compartments formed by organelles. Mitochondria serve to restore energy-rich compounds, while energy is mainly consumed in the cytosolic space. Molecules involved in these metabolic cycles have to cross the mitochondrial membrane via specialized carriers embedded in the inner mitochondrial membrane. All relevant structural and functional aspects of the **Anion Carriers of** **Mitochondrial Membranes** as well as isolation procedures, reconstitution experiments and biogenesis of the carriers, kinetics of transport as well as the metabolic regulation mechanisms are treated in this volume. Front Matter....Pages I-XI Front Matter....Pages 1-1 Purification and Characterization of Three Mitochondrial Substrate Carriers: the Phosphate, the 2-Oxoglutarate and the Dicarboxylate Carriers....Pages 3-15 Purification and Reconstitution of the 2-Oxoglutarate Carrier from Bovine Heart and Liver Mitochondria....Pages 17-34 Hydroxyapatite Chromatography as a Tool for the Isolation of Anion Carriers and Other Membrane Proteins....Pages 35-44 Purification of the Monocarboxylate Carrier by Affinity Chromatography....Pages 45-57 Recent Developments in the Extraction, Reconstitution, and Purification of the Mitochondrial Citrate Transporter from Normal and Diabetic Rats....Pages 59-69 Isolation and Functional Reconstitution of the Dicarboxylate Carrier from Bovine Liver Mitochondria....Pages 71-85 New Photoaffinity Derivatives of Malonate and Succinate to Study Mitochondrial Carrier Systems....Pages 87-97 Reaction Mechanism of the Reconstituted Aspartate/Glutamate Antiporter from Mitochondria. Reversible Switching to Uniport Function....Pages 99-110 Front Matter....Pages 111-111 Recent Studies on the Mitochondrial Phosphate Transport Protein (PTP) and on its Relationship to the ADP/ATP Translocase (AAC) and the Uncoupling Protein (UCP)....Pages 113-121 Mitochondrial Phosphate Carrier: Relation of its SH Groups to Oligomeric Organization....Pages 123-131 Recent Developments in the Study of the Conformational States and the Nucleotide Binding Sites of the ADP/ATP Carrier....Pages 133-146 Immunological and Enzymatic Approaches of the Orientation of the Membrane Bound ADP/ATP Carrier....Pages 147-157 The ATP/ADP Antiporter is Involved in the Uncoupling Effect of Fatty Acids....Pages 159-168 Molecular Aspects of the Adenine Nucleotide Carrier from Mitochondria....Pages 169-181 Kinetic Mechanisms of the Adenylic and the Oxoglutaric Carriers: a Comparison....Pages 183-195 Front Matter....Pages 197-197 Porins from Mitochondrial and Bacterial Outer Membranes: Structural and Functional Aspects....Pages 199-214 Modulation of the Mitochondrial Channel VDAC by a Variety of Agents....Pages 215-224 Bioenergetic Consequences of the Lack of Mitochondrial Porin: Identification of a Putative New Pore....Pages 225-235 Purification of Mammalian Porins....Pages 237-248 Front Matter....Pages 249-249 A Molecular Biology Study of the Uncoupling Protein of Brown Fat Mitochondria. A Contribution to the Analysis of Genes of Mitochondrial Carriers....Pages 251-260 Front Matter....Pages 249-249 On the Mechanism of Transport by the Uncoupling Protein from Brown Adipose Tissue Mitochondria....Pages 261-268 Regulation of the Amount and Activity of the Uncoupling Protein Thermogenin in Brown Adipose Tissue....Pages 269-281 Regulation of Uncoupling Protein and Formation of Thermogenic Mitochondria....Pages 283-291 Front Matter....Pages 293-293 Biogenesis of Mitochondrial Proteins....Pages 295-306 Insensitivity of Carbamoyl-Phosphate Synthetase Towards Inhibition by Carbamoyl Phosphate Makes it Unlikely that Mitochondrial Metabolite Transport Controls Ornithine Cycle Flux....Pages 307-315 Mitochondrial Adenine Nucleotide Translocation During Fatty Acid Metabolism in the Intact Cell....Pages 317-325 Control of Oxidative Phosphorylation in Yeast Mitochondria: The Role of Phosphate Carrier and pH....Pages 327-336 The Role of Pyrophosphate and the Adenine Nucleotide Transporter in the Regulation of the Intra-Mitochondrial Volume....Pages 337-348 Role of the Mitochondrial Outer Membrane in Dynamic Compartmentation of Adertine Nucleotides....Pages 349-359 Topology of Peripheral Kinases: its Importance in Transmission of Mitochondrial Energy....Pages 361-372 Control of Mitochondrial Energy Production in Vivo....Pages 373-381