Animal cell technology. Volume 5 : basic and applied aspects : proceedings of the Fifth International Meeting of the Japanese Association for Animal Cell Technology, Omiya, Japan, November 30-December 4, 1992

Animal cell technology has been making tremendous progress. Originally this term reminded people of engineering for high density and large volume culture of animal cells. At present many fields of biological sciences are aiming at advance in animal cell technology. Cell culture engineering is aided not only with developments in apparatus, matrix, media, and computational analysis, but also with new biological procedures in gene and protein technology, cell biological resources and immunological methods. Results obtained with animal cell technology are applied to production of pharmaceuticals, diagnosis reagents and food endowed with physiological functions, and cell and gene therapy of animals and humans, and useful for elucidating scientific phenomena. It is also essential to establish methods of evaluation for functionality and safety of newly discovered molecules and cells. The progress in animal cell technology is supported by, and attributes in both of basic and applied sciences. The proceedings of the Fifth International Meeting of the Japanese Association for Animal Cell Technology (JAACT) covers the subjects above mentioned. The articles in this book will help researchers in many fields to understand the current status and future trends in animal cell technology. JAACT organized this Meeting and we express our gratitude to the members of JAACT. We gratefully acknowledge all the members of the organizing committee for their dedication in assuring the Meeting's success. For their valuable supports, we also thank the Japanese Biolndustry Association and Saitama Foundation for Culture and Industry. Front Matter....Pages i-xvii Search for Edible Plant Constituents of Anti-Tumor Promotion Using an Inhibition Test of Epstein-Barr Virus Activation in Raji Cells....Pages 1-8 The Nature and Effect of Cell-Cell Interactions between Recombinant CHO Cells Producing Human Interferon-Gamma.....Pages 9-18 Characterization of Newly Established Cells Which Provide an Animal Model for Spontaneous Metastasis....Pages 19-24 Bovine Mammary Epithelial Cells: Morphology, Protein Synthesis and Gene Transfection....Pages 25-31 Growth and Differentiation of a Small Intestinal Epithelial Cell Line IEC-6....Pages 33-40 Subunit Assembly of Laminin Variants in Cultured Animal Cells....Pages 41-48 Regulation of Mitochondrial Gene Expression by Interferons....Pages 49-55 Production of the Human Growth Hormone in Serum-Free UC203 Medium....Pages 57-61 Stable Production of Pro-urokinase and its Derivative by Namalwa KJM-1 Cells Adapted to a Serum-free Medium....Pages 63-70 Production and Characterization of Recombinant Soluble form Erythropoietin Receptor....Pages 71-77 A ts-SV40 Based New Mammalian Plasmid Vector and Its Copy Number Control by Temperature Change....Pages 79-84 Selection of Regulatory Mutants of μ Gene Expression from Myeloma Cells Transfected with the Modified μ Gene....Pages 85-91 Process Control of BHK Cells by Nucleotide Pool Measurement....Pages 93-97 Purification and cDNA Cloning of Flatfish Interferon....Pages 99-105 Detection by Fluorescence in Situ Hybridization of the Erythropoietin Gene Introduced into Animal Cells....Pages 107-113 High-Level Expression of Recombinant Bovine ß-Lactoglobulin in Insect Cells....Pages 115-121 Mass Production and Purification of Recombinant Flatfish Interferon....Pages 123-127 Stimulation of Protein Degradation By Tumor Necrosis Factor a in Cultured Muscle Cells....Pages 129-133 From in Vitro Cell Culture to in Vivo Animal Study: Demonstration that Epidermal Growth Factor (EGF) Is a Potent Inhibitor of Adipose Differentiation.....Pages 135-141 Co-Operative Action of Cysteine and Some Hormones on Bile Acid Synthesis in Primary Cultured Rat Hepatocytes....Pages 143-148 Suppression of Killer T Cell Induction by Prodigiosin 25-C....Pages 149-154 Immunopotentiator from Bifidobacterium Adolescentis ....Pages 155-164 Immunosuppressive Properties of FK-506....Pages 165-169 Enhancement of Antibody Production and Cell Proliferation by Marine Bioactive Substances....Pages 171-176 Effect of a dialyzate of spinach on the chromosomal aberration induced by mutagens in cultured Chinese hamster cells....Pages 177-181 Suppression of Melanin Production in a Mouse B16 Melanoma 4A5 Cell Line by a Spinach Extract....Pages 183-188 Effect of Spinach Extracts on the Proliferation and on Some Phenotypic Characteristics of Human Myeloid Leukemia Cell Lines....Pages 189-193 Sustained Inducibility of Cytochrome P450 Activity in Rat Hepatocytes Cultured in a Serum-Free Medium....Pages 195-201 Biosynthesis of Protein Products by Animal Cells - Are Growth and Non-Growth Associated Concepts Valid or Useful?....Pages 203-213 Use of Liquid Medium Concentrates to Enhance Biological Productivity.....Pages 215-222 Optimisation of α-Interferon Expression of Namalwa Cells....Pages 223-230 Changes in the Glycosylation of Interferon-γ during Culture....Pages 231-235 Growth Control of Hybridoma Cultures by Adding or Depriving Interleukin-6, Caffeine, Amino Acids, Growth Factors, or Antisense C-myc....Pages 237-242 Chemostat Culture Studies on CHO Cells Producing Interferon-γ....Pages 243-250 Chinese Hamster Ovary (CHO) Cell Growth and Recombinant Protein Production in Serum-Free Media....Pages 251-257 Mechanism of IL-10 Production by a CD8 + T Cell Clone....Pages 259-266 Generation of CHO Cell Mutants for Growth Control....Pages 267-272 Removability and Permeability of DNA in a Solution by Cuprammonium Regenerated Cellulose Hollow Fiber (BMM TM ) for Process Validation of Purification Process of Bio-Drugs....Pages 273-279 Principles and Methods of Process Validation Studies for Virus Removal and Inactivation....Pages 281-288 Combination of Virus Removal and Inactivation for the Process Validation of Bio-Drugs....Pages 289-294 A New, Validated Method for the Destruction of Conventional Viruses and Unconventional Infectious Agents (e.g. BSE and Scrapie)’ in Proteins and Sera....Pages 295-300 Amino Acid Metabolism of a High Density Perfusion Culture....Pages 301-307 Kinetics of Cell Metabolism and Antibody Production in High Concentration Perfusion Cultures....Pages 309-324 Growth Yields of Hybridoma Cells and Monoclonal Antibody Production in High Density Culture....Pages 325-333 Towards a Strategy for High Density Cultures of Vero Cells in Stirred Tank Reactors....Pages 335-340 50L Scale Perfusion Culture of Hybridoma Cells by Gravitational Settling for Cell Separation....Pages 341-346 Production of Monoclonal Antibodies with a Radial-Flow Bioreactor....Pages 347-353 Factors Affecting the Yield of a Baculovirus Insect Cell System by Hollow-fiber Culture....Pages 355-360 High-Density Animal Cell Culture by Gas Sparging....Pages 361-367 Non-Woven Fabrics for Immobilizing Mammalian Cells....Pages 369-374 Statistical Design of Culture Medium for Recombinant Interferon-γ Production....Pages 375-381 Medium Optimization for Perfused Culture to Provide High Product Titer....Pages 383-390 eRDF Medium Enhances Antibody Production of IgG and IgM Secreting Hybridomas.....Pages 391-396 Serum-Free Media for Large-Scale Suspension Cultures....Pages 397-401 Suspension Culture of Mouse Hybridoma HB8852 Cells with a Protein-Free Medium....Pages 403-408 Improved Monoclonal Antibody Production via Controlled Feeding Strategies during Fedbatch Cultures of a Hybridoma Cell Line Utilizing Protein Free Media....Pages 409-415 Prediction of Disruption of Animal Cells by Laminar and Turbulent Flows....Pages 417-423 Fed-Batch Propagation of Mouse Hybridoma and Human Lymphoblastoid Cell Lines (LCLs)....Pages 425-431 Bioreactor with a Radial-flow Non-woven Fabric Bed for Animal Cell Culture....Pages 433-442 Partial Cell Culture Medium Recirculation Experience in Pilot Scale....Pages 443-446 Adaptation to Stirred Culture - A Study of a Moderately Shear Sensitive Cell Line (PQXB1/2).....Pages 447-454 Evaluation of optimal process parameters for the membrane dialysis bioreactor with integrated radial-flow fixed bed....Pages 455-462 High Cell Density Perfusion Culture of Hybridoma Cells for Production of Monoclonal Antibodies in the Celligen Packed Bed Reactor....Pages 463-469 Metabolic parameters of a hybridoma cell line in batch and continuous cultivation....Pages 471-478 Development of a New Disposable Culture System for Human Lymphokine-Activated Killer (LAK) Cells....Pages 479-486 New Method for Observing Cells Inside a Macroporous Microcarrier....Pages 487-491 Production of an α-Amidating Enzyme (α-AE) in Recombinant CHO Cells....Pages 493-499 Development of Instrumentation and Control Strategy in Bioreactor Culture of Hybridoma Cells....Pages 501-507 Effect of Dissolved Oxygen Concentration on Growth of Mouse-Mouse Hybridoma....Pages 509-514 Isolation of Autocrine Growth Factors Producing Mouse Hybridoma Cell Lines....Pages 515-520 Production of Multisubunit Particles for Use as Vaccines Using the Baculovirus Expression Vector System (BEVS)....Pages 521-528 Spontaneous Apoptosis in Mouse Hybridoma Culture....Pages 529-534 Cell Cycle Dynamics of Antibody Formation in Hybridomas....Pages 535-541 Effect of the Cell-Cycle Phase on Hybridoma Culture....Pages 543-549 Physiological Aspects of Scaling Up and Monitoring....Pages 551-556 A Comparative Study of the Immunopotentiating Activity of Adjuvant Formulations Containing Glycopeptides....Pages 557-564 Establishment and Characterization of Monoclonal Antibodies against Fish Virus ( Iridoviridae )....Pages 565-571 The Production of Recombinant Antibodies Using the Glutamine Synthetase (GS) System....Pages 573-577 Generation of Supranatural Antibodies by Modifying the L Chains: Cell Hybridization and Glycotechnology....Pages 579-583 Two-Stage Chemostat Studies of Hybridoma Growth, Nutrient Utilisation, and Monoclonal Antibody Production....Pages 585-594 The Use of Artificial Intelligence to Enhance the Accuracy of Hybridoma Growth and Metabolism....Pages 595-600 Immunoglobulin Production Stimulating Factor (IPSF) Derived from Namalwa Cells....Pages 601-607 One-Step Purification of F(ab’) 2 Fragments of Mouse Monoclonal Antibodies (Immunoglobulins G1 and M) by Hydrophobic Interaction HPLC....Pages 609-616 Application of Anti-Tetanus Human Monoclonal Antibodies....Pages 617-623 Genetically Engineered Antibodies....Pages 625-639 Applications of Human Monoclonal Antibodies to Various Clinical Uses....Pages 641-646 Reactivity of Human Monoclonal Antibody BD9-D12 to Cancer Cells and Tissues....Pages 647-652 Cell Culture in Europe: A Review of Today’s Market for Cell Culture Products and Services....Pages 653-658 Back Matter....Pages 659-668 This is the fifth volume of the series, highlighting the latest research in the field of animal cell technology. The most recent data on cell growth and productivity in various cell culture systems are provided, including systems for recombinant animal gene expression and production of monoclonal antibodies, extending the investigations in the previous volumes. In addition, application of cell culture systems in new areas are presented: biosafety testing, removal of microorganisms, and the assessment of physiologically functional substances in food and natural sources. Also, a special section deals with applications of human monoclonal antibodies. All those involved in animal cell culture will find this volume an invaluable source on current research. Proceedings of the Fourth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 13-15 November 1991