Animal Cell Technology: Products of Today, Prospects for Tomorrow (ESACT Proceedings)

Content: Front Matter , Page iii Copyright , Page iv European Society for Animal Cell Technology , Page v ESACT Executive Committee (9/91 - 5/93) , Page vi Organising Committee , Page vi ACKNOWLEDGEMENT , Page vii Presence and Future of Animal Cell Technology: Chairman's introduction to the 12th ESACT Meeting , Pages 3-9 , Wolf Berthold, Karl Thomae GmbH INTRODUCTION OF DNA INTO MAMMALIAN CELLS , Pages 13-23 , A.J. van der Eb Retrotargeting: Use of defective retroviral DNA fragments to improve recombinant protein production in mammalian cells , Pages 24-29 , F.M. Wurm, A. Johnson, T. Etcheverry, Y.S. Lie, C.J. Petropoulos RECEPTOR-MEDIATED GENE DELIVERY INTO MAMMALIAN CELLS , Pages 30-34 , Ernst Wagner, Matt Cotten, Christian Plank, Karl Mechtler, Kurt Zatloukal, Max L. Birnstiel Improvement of Cell Lines for Large-scale Mammalian Cell Culturing , Pages 35-42 , Kazuaki Kitano Drosophila cell lines as hosts for recombinant protein expression , Pages 43-49 , C. Cavegn, J. Young, M. Bertrand, A.R. Bernard IMMORTALISED CELL LINES FOR VIRUS DIAGNOSIS , Pages 50-54 , J. Clarke, C. MacDonald, U. Kreuzburg-Duffy, J.B. Griffiths SELECTION STRATEGIES FOR HIGHLY PRODUCTIVE RECOMBINANT CELL LINES , Pages 55-60 , Helen N. Brand, Stephen J. Froud, Hilary K. Metcalfe, Adekunle O. Onadipe, Angela Shaw, Ashley J. Westlake Population analysis of a recombinant Chinese hamster ovary cell line expressing recombinant human protein cultured in the presence and absence of methotrexate selective pressure , Pages 63-68 , M.S. Sinacore, S. Brodeur, S. Brennan, M. Fallon, S.R. Adamson, D. Cohen GENETIC AND BIOCHEMICAL ANALYSIS OF A MURINE HYBRIDOMA IN LONG-TERM CONTINUOUS CULTURE , Pages 69-75 , AJ Racher, GN Stacey, BJ Bolton, A Doyle, JB Griffiths DNA FINGERPRINTING AS A QUALITY CONTROL MARKER FOR THE GENETIC STABILITY OF PRODUCTION CELLS , Pages 76-79 , Iain Doherty, Kenneth T. Smith, Gillian M. Lees ANALYSIS OF SV40 EARLY REGION EXPRESSION IN IMMORTALIZED MOUSE MACROPHAGE CELL LINES , Pages 80-82 , Ute Ch.K. Kreuzburg-Duffy, Caroline MacDonald Stimulation of Murine Hybridomas by Cytokines , Pages 85-87 , J.H Dempsey, D.H. Glass, F.B. Ward THE USE OF 2-HYDROXY-2,4,6-CYCLOHEPTARIN-l-ONE (TROPOLONE) AS A REPLACEMENT FOR TRANSFERRIN , Pages 88-90 , Hilary Metcalfe, Ray P. Field, Stephen J. Froud Comparison of long R 3 IGF-1 with insulin in the support of cell growth and recombinant protein expression in CHO cells , Pages 91-95 , James N. Thomas, Victor Fung DEVELOPMENT OF A HYBRIDOMA CLONING MEDIUM , Pages 96-98 , R. Weedle, K. Carroll, K. Kavanagh, R. O'Kennedy, M. Clynes 241 9.22 Performance of Gamma-irradiated Fetal Bovine Serum in Cell Culture , Pages 99-101 , J.D. Keathley, D. Wyatt, C.M. Williams, R. Festen, C. Maben LOW-LEVEL FCS ADAPTATION AND MEDIA DEVELOPMENT FOR THE CULTURE OF TWO HYBRIDOMA CELL LINES PRODUCING IgG AND IgM MONOCLONAL ANTIBODIES , Pages 102-104 , A. Sanfeliu, B. Damgaard, J.J. Cairó, C. Casas, C. Solà, F. Gòdia Improved bioreactor productivity and manufacturing efficiency using liquid medium concentrates , Pages 105-109 , D.W. Jayme, R.M. Fike, J.M. Kubiak, P.J. Price Cell Culture Raw Materials Screening By Calcein-AM Fluorescence Using A 96 Well Plate Format , Pages 110-114 , T. Ihrig, M. Tsao, F. Jacobson, M.B. Sliwkowski, M. Hilton Importance of the extra-cellular matrix in the long term, anchorage dependent growth of cell line CHO Kl , Pages 115-117 , Michael Bridges, Carol Gebert, Peter P. Gray DEVELOPMENT OF SERUM FREE MEDIA FOR ENGINEERED NSO CELL LINES , Pages 121-123 , Claudia W. Buser, Rachelle Beaudet, Nancy Soohoo, Gordon G. Pugh Cholesterol: An important additive for serum-free media? , Pages 124-126 , H. Büntemeyer, M. Burg, J. Lehmann Serum-free UltraCULTURE™ medium : an alternative to the unstability generated by long-term cultivation of mouse hybridomas , Pages 127-129 , N. KESSLER, S. BERTRAND, G. THOMAS, M. AYMARD INVESTIGATIONS OF THE TSH ANTIBODY PRODUCING MOUSE HYBRIDOMA CELL LINE UNDER SERUM-FREE CONDITION , Pages 130-133 , E.-J. Schlaeger, T. Klier, K. Christensen INTERFERON GAMMA PRODUCTION IN LABORATORY BIOREACTOR BY Lens culinaris LECTIN STIMULATED HUMAN BUFFY COAT USING PROTEIN FREE MEDIUM , Pages 134-136 , Knezevic Miomir, Bratko Filipic, Christine Schmatz, Katarina Carlsson, Sonja Zupan, Peter Vrhovec, Martin Batic, Boris Kovac, Peter Raspor Evaluation of CHO Cell Growth in a Protein-free Environment , Pages 137-139 , DE Wyatt INACTIVATION OF SERUM CONTAMINANTS , Pages 140-143 , D.E. Wyatt, J. Keathley, C. Williams, R. Broce Adaptation of Mammalian Cells to Protein-Free Growth , Pages 144-146 , DE Wyatt AMMONIUM IONS INHIBIT CELL GROWTH BY INTRACELLULAR FORMATION OF UDP-ACTIVATED HEXOSAMINES , Pages 149-151 , Thomas Ryll, Roland Wagner Influence of the pH on the ammonia sensitivity of a murine hybridoma cell line , Pages 152-154 , Ines Lüdemann, Ralf Pörtner, Herbert Märkl THE INFLUENCE OF AMMONIUM ON t-PA PRODUCTION FROM CHINESE HAMSTER OVARY (CHO) CELLS , Pages 155-157 , C. Dyring, H.A. Hansen, C. Emborg Detoxification of ammonium in bioreactor cultivations , Pages 158-160 , Kristina Martinelle, Lena Häggström Influence of lactate and ammonia on the death rate of hybridoma , Pages 161-163 , J.L. GOERGEN, A. MARC, J.M. ENGASSER THE EFFECT OF GLUTAMINE-CONTAINING DIPEPTIDES ON THE GROWTH AND PRODUCTIVITY OF A HYBRIDOMA , Pages 164-166 , A. Christie, M. Butler Inhibitory Substance(s) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line , Pages 167-169 , S. Siwiora, M. Fingberg, H. Büntemeyer, J. Lehmann GROWTH OF HYBRIDOMA CELLS IS INHIBITED BY GANGLIOSIDES , Pages 170-172 , Heike Brandt, Johannes Müthing, Jürgen Lehmann Programmed to Die : Cell Death via Apoptosis , Pages 175-182 , Thomas G. Cotter CELL DEATH (APOPTOSIS) IN CELL CULTURE , Pages 183-186 , E.-J. Schlaeger, S.D. Simpson CELL DEATH BY NECROSIS AND APOPTOSIS DURING THE CULTURE OF COMMERCIALLY IMPORTANT CELL LINES , Pages 187-191 , R.P. Singh, M. Al-Rubeai, A.N. Emery, C.D. Gregory SPONTANEOUS APOPTOSIS CONTRIBUTES NEGATIVELY TO THE REGULATION OF HYBRIDOMA CELL VIABILITY , Pages 192-198 , F. Franěk Metabolic characteristics and specific requests of hybridoma and CHO cell lines , Pages 199-201 , P. Schorn, W. Noé, W. Berthold NATURE OF CELL-CELL INTERACTIONS BETWEEN RECOMBINANT CHO CELLS EXPRESSING HUMAN INTERFERON GAMMA , Pages 202-204 , S.R. Coppen, R. Newsam, A.J. Baines, A.T. Bull HIGH DENSITY INSECT CELL CULTURE FOR THE PRODUCTION OF RECOMBINANT PROTEINS WITH THE BACULOVIRUS EXPRESSION SYSTEM , Pages 207-211 , Volker Jäger, Anja Kobold, Christiane Köhne, Sven M. Deutschmann, Eckart Grabenhorst, Christine Karger, Harald S. Conradt THE SUPER-SPINNER A MASS CELL CULTURE BIOREACTOR FOR THE CO, INCUBATOR , Pages 212-214 , R. Heidemann, U. Riese, D. Lütkemeyer, H. Büntemeyer, J. Lehmann CONTINUOUS SUSPENSION CHO CELL CULTURE , Pages 215-217 , Henrik Albahn Hansen, Claus Emborg Progress in animal cell perfusion technology with Superspin , Pages 218-221 , R. Plüss, D. Doyen, L. van der Pol, J-L Romette PRODUCTION OF A RECOMBINANT PROTEIN IN A HIGH DENSITY INSECT CELL CYTOFLOW REACTOR , Pages 222-226 , F-X. Deramoudt, S. Monnet, J-N. Rabaud, J-M. Quiot, M. Cerutti, G. Devauchelle, M. Kaczorek A compact gravitational settling device for cell retention , Pages 227-229 , Keith J Thompson, James S Wilson Conical Bioreactor with Internal Lamella Settler for Perfusion Culture of Suspension Cells , Pages 230-233 , C. Knaack, G. André, C. Chavarie Lamellar Clarifier - A New Device for Animal Cell Retention in Perfusion Culture Systems , Pages 234-239 , J. Stevens, S. Eickel, U. Onken PERFUSION CULTURE OF SUSPENDED CHO CELLS EMPLOYING INCLINED SEDIMENTATION , Pages 240-242 , James A. Searles, Paul Todd, Dhinakar S. Kompala Collagenase production by a cell line in collagen microspheres in a fluidized-bed reactor and its modelization , Pages 245-247 , Th. Marique, Th. Paul, D. Blankaert, I. Texeira Guerra, J. Wérenne DEVELOPMENT OF A BIOREACTOR PROCESS FOR RECOMBINANT CELL LINES , Pages 248-251 , AJ Racher, Griffiths. JB GROWTH AND METABOLISM OF CHO-CELLS IN POROUS GLASS CARRIERS , Pages 252-255 , E. Lüllau, M. Biselli, C. Wandrey EVOLUTION OF THE CAPACITANCE OF CYTODEX 3 MICROBEADS SUSPENDED IN GROWTH MEDIUM , Pages 256-258 , V. Degouys, F.D. Menozzi, D.A. Dubois, L. Fabry, A.O.A. Miller HIGH DENSITY CULTURE USING MACROPOROUS MICROCARRIER , Pages 261-263 , J. Shirokaze, M. Nogawa, R. Ogura Growth of Spodoptera cells on porous microcarriers (Verax), production of recombinant protein , Pages 264-266 , G.R. Pettman, C.J. Mannix PROTEIN FREE CULTURE OF R-CHO AND HYBRIDOMA CELLS ON THE MACROPOROUS POLYPORE® CARRIER , Pages 267-269 , G. Blüml, M. Reiter, N. Zach, T. Gaida, C. Schmatz, A. Assadian, K. Strutzenberger, H. Katinger A NOVEL HEPATOCYTE ENTRAPMENT, HOLLOW FIBER BIOREACTOR AS A BIO-ARTIFICIAL LIVER , Pages 273-277 , M.V. Peshwa, S.L. Nyberg, F.J. Wu, Bruce Amiot, F.B. Cerra, W-S. Hu Pitfalls of bioprocessing a human monoclonal multireactive IgM antibody , Pages 278-280 , U. Marx, D. Roggenbuck, M. Wilding, H. Tanzmann, S. Jahn Hollow Fiber Cell Culture - A Proven Alternative , Pages 281-283 , Bud Harwath Development of a new membrane reactor for large scale mammalian cell culture , Pages 287-289 , J.L. GOERGEN, A. MARC, J.M. ENGASSER, J.N. RABAUD, G. PIERRY, V. GEAUGEY, I. GEAHEL, J. HACHE A SIMPLE MAGNETIC DRIVEN MINI-REACTOR FOR FLUIDIZED AND PACKED BEDS , Pages 290-292 , T. Gaida, W. Schich, M. Reiter, G. Blüml, N. Zach, H. Katinger Animal-Cell Cultivation in the NASA Rotating-Wall Vessel , Pages 293-295 , K.C. O'Connor, T.L. Prewett, T.J. Goodwin, K.M. Francis, A.D. Andrews, G.F. Spaulding HUMAN CANCER AND PRIMARY CELL CULTURE IN THE NEW HYBRID BIOREACTOR SYSTEM TECNOMOUSE , Pages 296-298 , A. Nagel, E. Effenberger, S. Koch, U. Marx, L. Lübbe Analysis of a long term hybridoma culture in a new minimized high cell density bioreactor , Pages 299-301 , S. Koch, H. Tanzmann, Ch. Riese, R.V. Baehr, U. Marx Cell-bubble interactions during aeration are strongly influenced by surfactants in the medium and can be minimized in the newly developed bubble bed reactor , Pages 302-304 , M. Jordan, H. Sucker, F. Widmer, H.M. Eppenberger MICRO-BUBBLE SPARGING OF ANCHORAGE DEPENDENT ANIMAL CELL CULTURES , Pages 305-308 , A. Handa-Corrigan, S. Nikolay, R. Brydges RATIONALIZATION OF THE DESIGN OF HIGH DENSITY PERFUSION CULTURES FOR SUSPENDED ANIMAL CELLS , Pages 309-311 , A. Handa-Corrigan, S. Nikolay, S. Zhang SIMULATION OF HYBRIDOMA GROWTH AND MONOCLONAL ANTIBODY PRODUCTION IN A HOMOGENOUS DIALYSIS BIOREACTOR , Page 312 , B. Szperalski, F. Geipel, T. Lorenz, U. Behrendt, J. Wahl, M. Comer Comparison of Bioreactor Technologies for Efficient Production of Recombinant α-Amidating Enzyme , Pages 315-320 , D.E. Matthews, K.E. Piparo, V.H. Burkett, C.R. Pray A COMPARISON OF ANIMAL CELL GROWTH USING MICROCARRIERS AND SUSPENDED NATURAL AGGREGATES , Pages 321-323 , P.M. Alves, J.L. Moreira, A.S. Feliciano, M.J.T. Carrondo COMPARATIVE STUDIES OF CELL PROPAGATION SYSTEMS FOR PRODUCTION OF HUMAN ACETYLCHOLINESTERASE BY RECOMBINANT CELLS , Pages 324-328 , A. Lazar, S. Reuveny, C. Kronman, B. Velan, A. Shafferman Production of a Monoclonal Antibody with a Serum Dependant Hybridoma in Different Reactor Systems , Pages 329-330 , Horst D. Blasey, Cedric O. Isch, Alain R. Bernard Transient expression with COS cells on spinner scale , Pages 331-332 , Horst D. Blasey, Alain R. Bernard PRODUCTION OF THE HIV-1 NEUTRALISING HUMAN MONOCLONAL ANTIBODY 2F5: STIRRED TANK VERSUS FLUIDIZED BED CULTURE , Pages 333-335 , M. Reiter, A. Buchacher, G. Blüml, N. Zach, W. Steinfellner, C. Schmatz, T. Gaida, A. Assadian, H. Katinger THE CYTOSENSOR™-MICROPHYSIOMETER: BIOLOGICAL APPLICATIONS OF LIVING CELLS ON SILICON SENSOR , Pages 339-344 , R. Metzger Mass spectroscopy determination of insect cell respiration rates in culture and production processes , Pages 345-350 , A.A. Kamen, R. Tom Intracellular pH evolution during batch cultures using flow cytometry , Pages 351-353 , M. Cherlet, N. Petitpain, M. Dardenne, P. Franck, J.M. Engasser, A. Marc CELL CYCLE ANALYSIS AS A TOOL FOR CONTROL AND REGULATION OF MAMMALIAN CELL CULTURES IN BIOREACTORS , Pages 354-356 , Sven M. Deutschmann, Ulrich Valley, Volker Jäger, Roland Wagner Use of MTT-assay in studies of the CHO:DG44 cell line , Pages 357-359 , Michael Hasenson On-line-measurements of metabolites in mammalian cell bioreactors by means of enzyme electrodes and of the number of living and dead cells , Pages 360-362 , U. Wellnitz, A. Rakow FULLY AUTOMATED IMAGE ANALYSIS OF CELL NUMBER, VIABILITY AND MORPHOLOGY , Pages 363-365 , K.G. Tucker, J. Welsh, M. Al-Rubeai, C.R. Thomas Fast Quantification of Mouse IgG from Cell Culture Supernatants , Pages 366-368 , Dietmar Schell On-line monitoring of growth and viral infection of VERO cells in collagen microspheres in a fluidized-bed bioreactor , Pages 369-371 , Th. Marique, D. Malarme, P. Stragier, J. Wérenne ON-LINE GLUCOSE CONTROL OF ANIMAL CELL CULTURES IN FLUIDIZED BEDS , Pages 372-375 , A.P. Loibner, N. Zach, O. Doblhoff-Dier, M. Reiter, K. Bayer, H. Katinger On-line indirect measurement of oxygen uptake rate in bench scale hybridoma cultures , Pages 376-378 , L.K. Nielsen, P.F. Greenfield Software sensors for the monitoring of high-density hybridoma perfusion cultures , Pages 379-382 , F. PELLETIER, C. FONTEIX, A. LOURENÇO da SILVA, J.L. GOERGEN, A. MARC, J.M. ENGASSER Application of an industrial disc stack centrifuge for the separation of hybridoma cells as a first step in downstream processing , Pages 383-385 , R. Kempken, A. Preißmann, J. Schäfer, W. Berthold DYNAMIC INTERFACIAL TENSION AND RHEOLOGICAL PROPERTIES OF CELL CULTURE MEDIUM WITH SHEAR PROTECTANT ADDITIVES , Pages 389-391 , James Michaels, Ameet K. Mallik, Jason E. Nowak, Darsh T. Wasan, E.T. Papoutsakis MIXING PHENOMENA IN INDUSTRIAL BIOREACTORS WITH PERFUSION SPIN FILTERS , Pages 392-396 , K. Jim Jem, Seif Fateen, James Michaels HYDRODYNAMIC CONTROL OF SUSPENDED NATURAL ANIMAL CELL AGGREGATES , Pages 397-401 , J.L. Moreira, A.S. Feliciano, P.M. Alves, J.G. Aunins, M.J.T. Carrondo MODELLING AND PREDICTION OF ANIMAL CELL DISRUPTION IN SIMPLE FLOWS , Pages 402-404 , Z. Zhang, C. Born, M. Al-Rubeai, C.R. Thomas BIOSYNTHETIC RESPONSES AND SCANNING ELECTRON MICROSCOPIC STUDIES OF MURINE HYBRIDOMAS SUBJECTED TO HYDRODYNAMIC STRESSES , Pages 405-409 , Steve K.W. Oh, Mohamed Al-Rubeai, Nick Emery, Alvin Nienow Fed-batch Strategies for Mammalian Cell Cultures , Pages 413-418 , W. Noe, P. Schorn, R. Bux, W. Berthold GROWTH AND PRODUCTIVITY OF ANIMAL CELLS: A CONTRIBUTION TO SOME FACTORS INFLUENCING BOTH , Pages 419-421 , G. Kretzmer, A. Ludwig, R. Weidemann, M. Teige, J. Tomeczkowski Simultaneous Control of Growth and Productivity Using a Mutant CHO Cell Line , Pages 422-424 , Alison Hovey, Chris Bebbington, Nigel Jenkins FACTORS AFFECTING GROWTH, INFECTION AND PROTEIN YIELD IN BACULOVIRUS -INFECTED INSECT CELLS , Pages 425-427 , N. Kioukia, M. Al-Rubeai, A.W. Nienow, A.N. Emery Effects of the feed medium glucose concentration on growth and metabolism of hybridomas in chemostat culture , Pages 428-430 , J.J. Meijer, J.P. van Dijken, K.Ch.A.M. Luyben Effects of Glucose on the Production of Recombinant Protein C in Mammalian Cell Culture , Pages 431-433 , Takeyuki Sugiura Substrate level control in fed-batch hybridoma cultures , Pages 434-436 , B. Romein, P. van Londen, J.J. Meijer, C. Hellinga, J.P. van Dijken, K.Ch.A.M Luyben Effect of Dissolved Oxygen on Monoclonal Antibody Production from Hybridoma Cultured in Haemodialysers , Pages 437-456 , G.B. Ryan, M.T. Simpson, W.T. Jones, M.J. Nicol, P.H.S. Reynolds Metabolic parameters of a hybridoma cell line in batch and continuous cultivation , Pages 457-459 , Ralf Pörtner, Armin Bohmann, Ines Lüdemann, Herbert Märkl Overproduction Of Monoclonal Antibodies In High Salt Medium In The Presence of Butyrate , Pages 460-464 , Steve K.W. Oh, Pooja Vig, Florence Chua, W.K. Teo, Miranda G.S. Yap GROWTH CONTROL OF PRODUCTION PROCESSES WITH BHK CELLS AND REGULATION OF THE PERFUSION RATE BY MONITORING THE INTRACELLULAR NUCLEOTIDE POOLS , Pages 465-469 , Ulrich Valley, Thomas Ryll, Roland Wagner Control of the maximal cell density in a membrane perfusion reactor , Pages 470-475 , H. PINTON, A. LOURENCO da SILVA, J.L. GOERGEN, A. MARC, J.M. ENGASSER, J.N. RABAUD, G. PIERRY Continuous Medium Recycling in Pilot Scale with a Recombinant Animal Cell Culture for Antithrombin III Production , Pages 476-480 , U. Riese, D. Lutkemeyer, R. Heidemann, H. Büntemeyer, J. Lehmann OPTIMIZATION OF ANTIBODY PRODUCTION IN A FLUIDIZED BED BIOREACTOR , Pages 481-484 , G. Rolef, M. Biselli, R. Dunker, C. Wandrey MONOCLONAL ANTIBODIES RELEASED FROM VIABLE HYBRIDOMA CELLS AT DIFFERENT STAGES OF GROWTH , Pages 485-493 , H. Musielski, K. Rüger, M. Zwanzig, K. Lehmann HIGH CELL DENSITY IN INSECT CELL CULTURES , Pages 494-503 , S. Reuveny, C.W. Kemp, J. Shiloach Semi-continuous production with the baculovirus expression system , Pages 504-506 , Frank L.J. van Lier, Menno M. Engelkes, Leo A. van der Pol, Cornelis D. de Gooijer, Johannes Tramper LONG TERM CULTURES OF BHK SUSPENDED AGGREGATES , Pages 507-510 , J.L. Moreira, A.S. Feliciano, J.G. Aunins, M.J.T. Carrondo Batch Kinetic Data of Hybridoma Growth and Productivity as a Basis for Simulation of Antibody Production in Different Culture Systems , Pages 513-517 , Jörg Thömmes, Manfred Biselli, Maria-Regina Kula, Christian Wandrey Modelling insect cell cultures infected with recombinant baculovirus , Pages 518-520 , L.K. Nielsen, J. Power, K. Radford, S. Reid, P.F. Greenfield KINETIC ANALYSIS OF HYBRIDOMA GROWTH AND MONOCLONAL ANTIBODY PRODUCTION IN SEMICONTINUOUS AND CONTINUOUS CULTURES WITH RESPECT TO BATCH CULTURES , Pages 521-531 , O-W MERTEN, D MOEURS, H KELLER, M LENO, L CABANIE, E COUVE PROCESS DEVELOPMENT AND KINETIC ANALYSIS OF A PROTEIN-FREE HYBRIDOMA PERFUSION SYSTEM , Pages 532-538 , Sylvain Mercille, Mark Johnson, Lucie Bourget, Réal Lemieux, Bernard Massie Steady-state analysis of hybridomas in chemostat culture , Pages 539-541 , J.J. Meijer, B. Romein, J.P. van Dijken, K.Ch.A.M. Luyben KINETIC STUDIES OF FED-BATCH HYBRIDOMA CULTURES: EFFECT OF VARIOUS FEEDING COMPOSITIONS AND FLOW RATES , Pages 542-544 , M. DARDENNE, M. CHERLET, J.M. ENGASSER, A. MARC A general two-step procedure for the kinetic modelling of animal cell cultures , Pages 545-547 , V. Chotteau, G. Bastin Mathematical Modeling of the Growth and Production Kinetics in Hybridoma Cultures , Pages 548-550 , R.K. Biener, R. King, M. Howaldt, W. Noé, E.D. Gilles Impact of Improved Chromatographic Media on Productivity and Process Design in Downstream Processing , Pages 553-556 , Carina Schmidt, Jan H. Berglöf, Lars-Olof Lindquist Direct Capture of Recombinant Proteins from Animal Cell Culture using a Fluidized Bed , Pages 557-562 , John C. Erickson, John D. Finch, David C. Greene Chromatographic Separation of Immunoglobulins on a New “Tentacle”-type “TA”-Phase: Fractogel EMD TA 650(S) , Pages 563-566 , W. Brümmer, E. Müller, W. Müller, E. Merck VALIDATION OF CHROMATOGRAPHIC PROCEDURES USED IN THE FRACTIONATION OF PLASMA DERIVATIVES , Pages 567-570 , A.J. Darling, U.Ch.K. Kreuzburg-Duffy, L. Borland, A.E. Morrow, J.H. Berglöf, I. Andersson Successful products and future business prospects , Pages 573-579 , Rolf G. Werner PRODUCTION OF RECOMBINANT FACTOR VIII FROM PERFUSION CULTURES: I. LARGE-SCALE FERMENTATION , Pages 580-583 , B.G.D. Bödeker, R. Newcomb, P. Yuan, A. Braufman, W. Kelsey PRODUCTION OF RECOMBINANT FACTOR VIII FROM PERFUSION CULTURES: II. LARGE-SCALE PURIFICATION , Pages 584-590 , B.G.D. Bödeker, E. Potere, G. Dove ADVANTAGES OF HIGH CELL DENSITY IN VITRO CULTURES IN DOWN STREAM PROCESSING OF A MONOCLONAL IgG ANTIBODY FOR IN VIVO USE , Pages 591-593 , P. Preikschat, U. Marx, R.v. Baehr Production, purification and application of flatfish interferon , Pages 594-602 , Hiroki Murakami, Tadakazu Tamai, Tohru Noguchi, Nobuyuki Sato, Shouji Kimura, Sanetaka Shirahata Monoclonal Antibody Production srategy for serum dependent clone , Pages 603-604 , Cedric Isch, Alain R. Bernard, Horst D. Blasey HIGH LEVEL MEASLES VIRUS N PROTEIN EXPRESSION USING A RECOMBINANT BACULOVIRUS: ASSEMBLY OF NUCLEOCAPSID-LIKE STRUCTURES , Pages 605-609 , Anthony R. Fooks, John R. Stephenson, Alan Warnes, Barry Dowsett, Gavin W. Wilkinson, Bert K. Rima EQUIMOLAR EXPRESSION OF TWO PROTEIN CHAINS IN RECOMBINANT MAMMALIAN CELLS , Pages 610-616 , Wilhelm Dirks, Hansjörg Hauser Expression of Mammalian Protein Farnesyltransferase in a Baculovirus System , Pages 617-618 , John F. Moomaw, Patrick J. Casey, Wen-Ji Chen, Laurie Overton, Thomas A. Kost LEVELS OF TROPONIN I AND MYOSIN HEAVY CHAIN IN DEDIFFERENTIATED ADULT RABBIT CARDIAC MYOCYTES , Pages 619-621 , I. McPhee, K.A. Kane, C. MacDonald EXPRESSION OF THE D 2 AND D 3 DOPAMINE RECEPTORS IN INSECT CELLS USING THE BACULOVIRUS SYSTEM , Pages 622-624 , C. Woodcock, P.G. Strange, B.C. Rooney PRODUCTION AND CHARACTERIZATION OF SOLUBLE MOUSE AND HUMAN INTERFERON-γ RECEPTORS (IFNγ-R) FROM INSECT CELLS , Pages 625-632 , Georg Schmid, Nathalie Wild, Michael Fountoulakis, Harald Gallati, Reiner Gentz, Laurence Ozmen, Gianni Garotta, F. Hoffmann-La Generation of DNA-packaging proteins by overexpression in the Baculovirus/insect cell system , Pages 633-637 , V. Sandig, J. Forstova, N. Krauzewicz, B.E. Griffin, M. Strauss Metalloproteinases and TIMPs production by AIDS-Kaposi sarcoma derived cells , Pages 638-642 , D. Blankaert, D. Parent, C.M. Farber, J.P. van Vooren, C. Liesnard, J. Wérenne Endothelial cells and their infection with the rickettsia Cowdria ruminantium : prospects for vaccine production , Pages 643-645 , Ph. Totté, D. Blankaert, T. Marique, N. Vachiery, Guerra I. Teixeira, J. Werenne, C. Kirkpatrick, J.P. Vanvooren CHANGES IN THE GLYCOSYLATION PATTERN OF RECOMBINANT PROTEINS EFFECTED BY DEFINED CULTURE CONDITIONS OF BHK-21 CELLS , Pages 649-651 , Martin Gawlitzek, Roland Wagner, Harald S. Conradt, Corinne Villers, André Verbert Glycosylation analysis of a human monoclonal IgG antibody derived from a human- mouse heterohybridoma , Pages 652-657 , H. Leibiger, A. Hansen, H. Böhme, U. Marx Culture pH and ammonia affect expression rates and glycosylation of recombinant mouse placental lactogen proteins by CHO cells , Pages 658-660 , E.T. Papoutsakis, D.I.H. Linzer, M.C. Borys Analysis of the product consistency: Independent of process parameters, rt-PA shows a stable glycosylation pattern , Pages 661-666 , Kristina Kopp, Wolfgang Noé, Michael Schlüter, Franz Walz, Rolf Werner GALACTOSYLATION OF HUMAN/MOUSE IGG ANTI–NP ANTIBODIES AND ITS DETECTION BY ELISA , Pages 667-669 , M. Goodall, T. Bentley, J. Lund, R. Jefferis, N. Takahashi Characterization of carbohydrate chain on light chain of human monoclonal antibody , Pages 670-675 , Hiroki Murakami, Hirofumi Tachibana Comparison of the posttranslational modifications of human proteins secreted from different recombinant baculovirus infected insect cell lines , Pages 676-678 , Eckart Grabenhorst, Albrecht Gröner, Volker Jäger, Marcus Ackermann, Christine Karger, Kathrin Rollwage, Manfred Nimtz, Harald S. Conradt CHARACTERIZATION OF INTRA- AND EXTRACELLULAR PROTEASES IN RECOMBINANT MAMMALIAN AND HYBRIDOMA CELLS , Pages 679-682 , Ricardo B. Kratje, Waldemar Lind, Roland Wagner IMPACT OF FACTORS SECRETED BY MAMMALIAN AND INSECT CELLS ON HUMAN MONOCLONAL ANTIBODY INTEGRITY , Pages 683-687 , Marcus Ackermann, Volker Jäger, Uwe Marx BiP - a chaperone involved in antibody formation , Pages 688-693 , INGRID G. HAAS, CORNELIA R. KALOFF, MICHAEL R. KNITTLER Cell systems for in vitro toxicology , Pages 697-710 , Herbert Georg Miltenburger HEPATOCYTES: in vitro PHARMACOLOGY, TOXICOLOGY AND DRUG METABOLISM , Pages 711-722 , J.V. Castell, M.J. Gómez-Lechón, M.T. Donato Development of an Optimised in vitro System for Measurement of Human Tumour Response to Cytotoxic Agents , Pages 723-728 , C. Mothersill, M. Sheridan, J. Harney, J. Bonnar, T.P. Hennessy, C.B. Seymour Threedimensional cell cultures for toxicity testing and for the estimation of the affinity of cells to biocompatible materials? , Pages 729-731 , U. Wellnitz, A. Rakow Stable Expression of the Bovine Adrenal 11ß-Hydroxylase, a Cytochrome P 450 Enzyme, in CHO Cells as Tool for Drug Characterization , Pages 732-734 , Thomas Petri, Manfred Husemann ACTIVATION AND EXPANSION OF HUNAN CYTOTOXIC T LYMPHOCYTES IN HOLLOW FIBER BIOREACTORS , Pages 735-737 , C.H.J. Lamers, R.J. van de Griend, J.W. Gratama, R.L.H. Bolhuis Significance of contamination with viruses of cell lines used in the production of biological medicinal products , Pages 741-750 , P D Minor RESIDUAL IN VITRO ACTIVITY OF DNA IN BIOLOGICALS TREATED BY BETA-PROPIOLACTONE: RABIES VACCINES AS MODEL , Pages 751-753 , S. Morgeaux, N. Tordo, O. Merten, P. Perrin The development of appropriate viral models for the validation of viral inactivation procedures , Pages 754-756 , C.Z. Tao, R. Cameron, C. Harbour, J.P. Barford REGULATORY AFFAIRS SAFETY TESTING FOR GENE THERAPY PRODUCTS , Pages 757-759 , Exeen Morgan, Jeffrey Ostrove PRODUCT QUALITY STUDIES OF ANTITHROMBIN III DURING MEDIUM RECYCLING IN PILOT SCALE , Pages 760-762 , D. Lütkemeyer, U. Riese, R. Heidemann, M. Bretschneider, J. Lehmann, H.S. Conradt Product Consistency During Long-Term Fed-Batch Culture , Pages 763-768 , D.K. Robinson, C.P. Chan, T.C. Seamans, D.K. Lee, A.B. Lenny, J.-S. Tung, D.J. DiStefano, S. Munshi, S.L. Gould, G.E. Mark, C.C. Yu Ip, P.K. Tsai, J. Irwin, M. Silberklang THE APPLICATION OF INSECT CELLS FOR BIOPHARMACEUTICAL PRODUCTION: IMPLICATIONS FOR SAFETY TESTING , Pages 769-774 , C. McLean, A.J. Shepherd Generation of Linear Epitope Specific Antibodies Against the Human Recombinant Tissue-Plasminogen-Activator (rt-PA) , Pages 775-778 , Frank Hanakam, Günther Jung, Wolfgang Berthold, Rolf G. Werner, William Werz List of Participants , Pages 779-815 AUTHOR INDEX , Pages 816-819 Index , Pages 820-830 Content: Front Matter , Page iii Copyright , Page iv European Society for Animal Cell Technology , Page v ESACT Executive Committee (9/91 - 5/93) , Page vi Organising Committee , Page vi ACKNOWLEDGEMENT , Page vii Presence and Future of Animal Cell Technology: Chairman's introduction to the 12th ESACT Meeting , Pages 3-9 , Wolf Berthold, Karl Thomae GmbH INTRODUCTION OF DNA INTO MAMMALIAN CELLS , Pages 13-23 , A.J. van der Eb Retrotargeting: Use of defective retroviral DNA fragments to improve recombinant protein production in mammalian cells , Pages 24-29 , F.M. Wurm, A. Johnson, T. Etcheverry, Y.S. Lie, C.J. Petropoulos RECEPTOR-MEDIATED GENE DELIVERY INTO MAMMALIAN CELLS , Pages 30-34 , Ernst Wagner, Matt Cotten, Christian Plank, Karl Mechtler, Kurt Zatloukal, Max L. Birnstiel Improvement of Cell Lines for Large-scale Mammalian Cell Culturing , Pages 35-42 , Kazuaki Kitano Drosophila cell lines as hosts for recombinant protein expression , Pages 43-49 , C. Cavegn, J. Young, M. Bertrand, A.R. Bernard IMMORTALISED CELL LINES FOR VIRUS DIAGNOSIS , Pages 50-54 , J. Clarke, C. MacDonald, U. Kreuzburg-Duffy, J.B. Griffiths SELECTION STRATEGIES FOR HIGHLY PRODUCTIVE RECOMBINANT CELL LINES , Pages 55-60 , Helen N. Brand, Stephen J. Froud, Hilary K. Metcalfe, Adekunle O. Onadipe, Angela Shaw, Ashley J. Westlake Population analysis of a recombinant Chinese hamster ovary cell line expressing recombinant human protein cultured in the presence and absence of methotrexate selective pressure , Pages 63-68 , M.S. Sinacore, S. Brodeur, S. Brennan, M. Fallon, S.R. Adamson, D. Cohen GENETIC AND BIOCHEMICAL ANALYSIS OF A MURINE HYBRIDOMA IN LONG-TERM CONTINUOUS CULTURE , Pages 69-75 , AJ Racher, GN Stacey, BJ Bolton, A Doyle, JB Griffiths DNA FINGERPRINTING AS A QUALITY CONTROL MARKER FOR THE GENETIC STABILITY OF PRODUCTION CELLS , Pages 76-79 , Iain Doherty, Kenneth T. Smith, Gillian M. Lees ANALYSIS OF SV40 EARLY REGION EXPRESSION IN IMMORTALIZED MOUSE MACROPHAGE CELL LINES , Pages 80-82 , Ute Ch.K. Kreuzburg-Duffy, Caroline MacDonald Stimulation of Murine Hybridomas by Cytokines , Pages 85-87 , J.H Dempsey, D.H. Glass, F.B. Ward THE USE OF 2-HYDROXY-2,4,6-CYCLOHEPTARIN-l-ONE (TROPOLONE) AS A REPLACEMENT FOR TRANSFERRIN , Pages 88-90 , Hilary Metcalfe, Ray P. Field, Stephen J. Froud Comparison of long R 3 IGF-1 with insulin in the support of cell growth and recombinant protein expression in CHO cells , Pages 91-95 , James N. Thomas, Victor Fung DEVELOPMENT OF A HYBRIDOMA CLONING MEDIUM , Pages 96-98 , R. Weedle, K. Carroll, K. Kavanagh, R. O'Kennedy, M. Clynes 241 9.22 Performance of Gamma-irradiated Fetal Bovine Serum in Cell Culture , Pages 99-101 , J.D. Keathley, D. Wyatt, C.M. Williams, R. Festen, C. Maben LOW-LEVEL FCS ADAPTATION AND MEDIA DEVELOPMENT FOR THE CULTURE OF TWO HYBRIDOMA CELL LINES PRODUCING IgG AND IgM MONOCLONAL ANTIBODIES , Pages 102-104 , A. Sanfeliu, B. Damgaard, J.J. Cairó, C. Casas, C. Solà, F. Gòdia Improved bioreactor productivity and manufacturing efficiency using liquid medium concentrates , Pages 105-109 , D.W. Jayme, R.M. Fike, J.M. Kubiak, P.J. Price Cell Culture Raw Materials Screening By Calcein-AM Fluorescence Using A 96 Well Plate Format , Pages 110-114 , T. Ihrig, M. Tsao, F. Jacobson, M.B. Sliwkowski, M. Hilton Importance of the extra-cellular matrix in the long term, anchorage dependent growth of cell line CHO Kl , Pages 115-117 , Michael Bridges, Carol Gebert, Peter P. Gray DEVELOPMENT OF SERUM FREE MEDIA FOR ENGINEERED NSO CELL LINES , Pages 121-123 , Claudia W. Buser, Rachelle Beaudet, Nancy Soohoo, Gordon G. Pugh Cholesterol: An important additive for serum-free media? , Pages 124-126 , H. Büntemeyer, M. Burg, J. Lehmann Serum-free UltraCULTURETM medium : an alternative to the unstability generated by long-term cultivation of mouse hybridomas , Pages 127-129 , N. KESSLER, S. BERTRAND, G. THOMAS, M. AYMARD INVESTIGATIONS OF THE TSH ANTIBODY PRODUCING MOUSE HYBRIDOMA CELL LINE UNDER SERUM-FREE CONDITION , Pages 130-133 , E.-J. Schlaeger, T. Klier, K. Christensen INTERFERON GAMMA PRODUCTION IN LABORATORY BIOREACTOR BY Lens culinaris LECTIN STIMULATED HUMAN BUFFY COAT USING PROTEIN FREE MEDIUM , Pages 134-136 , Knezevic Miomir, Bratko Filipic, Christine Schmatz, Katarina Carlsson, Sonja Zupan, Peter Vrhovec, Martin Batic, Boris Kovac, Peter Raspor Evaluation of CHO Cell Growth in a Protein-free Environment , Pages 137-139 , DE Wyatt INACTIVATION OF SERUM CONTAMINANTS , Pages 140-143 , D.E. Wyatt, J. Keathley, C. Williams, R. Broce Adaptation of Mammalian Cells to Protein-Free Growth , Pages 144-146 , DE Wyatt AMMONIUM IONS INHIBIT CELL GROWTH BY INTRACELLULAR FORMATION OF UDP-ACTIVATED HEXOSAMINES , Pages 149-151 , Thomas Ryll, Roland Wagner Influence of the pH on the ammonia sensitivity of a murine hybridoma cell line , Pages 152-154 , Ines Lüdemann, Ralf Pörtner, Herbert Märkl THE INFLUENCE OF AMMONIUM ON t-PA PRODUCTION FROM CHINESE HAMSTER OVARY (CHO) CELLS , Pages 155-157 , C. Dyring, H.A. Hansen, C. Emborg Detoxification of ammonium in bioreactor cultivations , Pages 158-160 , Kristina Martinelle, Lena Häggström Influence of lactate and ammonia on the death rate of hybridoma , Pages 161-163 , J.L. GOERGEN, A. MARC, J.M. ENGASSER THE EFFECT OF GLUTAMINE-CONTAINING DIPEPTIDES ON THE GROWTH AND PRODUCTIVITY OF A HYBRIDOMA , Pages 164-166 , A. Christie, M. Butler Inhibitory Substance(s) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line , Pages 167-169 , S. Siwiora, M. Fingberg, H. Büntemeyer, J. Lehmann GROWTH OF HYBRIDOMA CELLS IS INHIBITED BY GANGLIOSIDES , Pages 170-172 , Heike Brandt, Johannes Müthing, Jürgen Lehmann Programmed to Die : Cell Death via Apoptosis , Pages 175-182 , Thomas G. Cotter CELL DEATH (APOPTOSIS) IN CELL CULTURE , Pages 183-186 , E.-J. Schlaeger, S.D. Simpson CELL DEATH BY NECROSIS AND APOPTOSIS DURING THE CULTURE OF COMMERCIALLY IMPORTANT CELL LINES , Pages 187-191 , R.P. Singh, M. Al-Rubeai, A.N. Emery, C.D. Gregory SPONTANEOUS APOPTOSIS CONTRIBUTES NEGATIVELY TO THE REGULATION OF HYBRIDOMA CELL VIABILITY , Pages 192-198 , F. Franěk Metabolic characteristics and specific requests of hybridoma and CHO cell lines , Pages 199-201 , P. Schorn, W. Noé, W. Berthold NATURE OF CELL-CELL INTERACTIONS BETWEEN RECOMBINANT CHO CELLS EXPRESSING HUMAN INTERFERON GAMMA , Pages 202-204 , S.R. Coppen, R. Newsam, A.J. Baines, A.T. Bull HIGH DENSITY INSECT CELL CULTURE FOR THE PRODUCTION OF RECOMBINANT PROTEINS WITH THE BACULOVIRUS EXPRESSION SYSTEM , Pages 207-211 , Volker Jäger, Anja Kobold, Christiane Köhne, Sven M. Deutschmann, Eckart Grabenhorst, Christine Karger, Harald S. Conradt THE SUPER-SPINNER A MASS CELL CULTURE BIOREACTOR FOR THE CO, INCUBATOR , Pages 212-214 , R. Heidemann, U. Riese, D. Lütkemeyer, H. Büntemeyer, J. Lehmann CONTINUOUS SUSPENSION CHO CELL CULTURE , Pages 215-217 , Henrik Albahn Hansen, Claus Emborg Progress in animal cell perfusion technology with Superspin , Pages 218-221 , R. Plüss, D. Doyen, L. van der Pol, J-L Romette PRODUCTION OF A RECOMBINANT PROTEIN IN A HIGH DENSITY INSECT CELL CYTOFLOW REACTOR , Pages 222-226 , F-X. Deramoudt, S. Monnet, J-N. Rabaud, J-M. Quiot, M. Cerutti, G. Devauchelle, M. Kaczorek A compact gravitational settling device for cell retention , Pages 227-229 , Keith J Thompson, James S Wilson Conical Bioreactor with Internal Lamella Settler for Perfusion Culture of Suspension Cells , Pages 230-233 , C. Knaack, G. André, C. Chavarie Lamellar Clarifier - A New Device for Animal Cell Retention in Perfusion Culture Systems , Pages 234-239 , J. Stevens, S. Eickel, U. Onken PERFUSION CULTURE OF SUSPENDED CHO CELLS EMPLOYING INCLINED SEDIMENTATION , Pages 240-242 , James A. Searles, Paul Todd, Dhinakar S. Kompala Collagenase production by a cell line in collagen microspheres in a fluidized-bed reactor and its modelization , Pages 245-247 , Th. Marique, Th. Paul, D. Blankaert, I. Texeira Guerra, J. Wérenne DEVELOPMENT OF A BIOREACTOR PROCESS FOR RECOMBINANT CELL LINES , Pages 248-251 , AJ Racher, Griffiths. JB GROWTH AND METABOLISM OF CHO-CELLS IN POROUS GLASS CARRIERS , Pages 252-255 , E. Lüllau, M. Biselli, C. Wandrey EVOLUTION OF THE CAPACITANCE OF CYTODEX 3 MICROBEADS SUSPENDED IN GROWTH MEDIUM , Pages 256-258 , V. Degouys, F.D. Menozzi, D.A. Dubois, L. Fabry, A.O.A. Miller HIGH DENSITY CULTURE USING MACROPOROUS MICROCARRIER , Pages 261-263 , J. Shirokaze, M. Nogawa, R. Ogura Growth of Spodoptera cells on porous microcarriers (Verax), production of recombinant protein , Pages 264-266 , G.R. Pettman, C.J. Mannix PROTEIN FREE CULTURE OF R-CHO AND HYBRIDOMA CELLS ON THE MACROPOROUS POLYPORE® CARRIER , Pages 267-269 , G. Blüml, M. Reiter, N. Zach, T. Gaida, C. Schmatz, A. Assadian, K. Strutzenberger, H. Katinger A NOVEL HEPATOCYTE ENTRAPMENT, HOLLOW FIBER BIOREACTOR AS A BIO-ARTIFICIAL LIVER , Pages 273-277 , M.V. Peshwa, S.L. Nyberg, F.J. Wu, Bruce Amiot, F.B. Cerra, W-S. Hu Pitfalls of bioprocessing a human monoclonal multireactive IgM antibody , Pages 278-280 , U. Marx, D. Roggenbuck, M. Wilding, H. Tanzmann, S. Jahn Hollow Fiber Cell Culture - A Proven Alternative , Pages 281-283 , Bud Harwath Development of a new membrane reactor for large scale mammalian cell culture , Pages 287-289 , J.L. GOERGEN, A. MARC, J.M. ENGASSER, J.N. RABAUD, G. PIERRY, V. GEAUGEY, I. GEAHEL, J. HACHE A SIMPLE MAGNETIC DRIVEN MINI-REACTOR FOR FLUIDIZED AND PACKED BEDS , Pages 290-292 , T. Gaida, W. Schich, M. Reiter, G. Blüml, N. Zach, H. Katinger Animal-Cell Cultivation in the NASA Rotating-Wall Vessel , Pages 293-295 , K.C. O'Connor, T.L. Prewett, T.J. Goodwin, K.M. Francis, A.D. Andrews, G.F. Spaulding HUMAN CANCER AND PRIMARY CELL CULTURE IN THE NEW HYBRID BIOREACTOR SYSTEM TECNOMOUSE , Pages 296-298 , A. Nagel, E. Effenberger, S. Koch, U. Marx, L. Lübbe Analysis of a long term hybridoma culture in a new minimized high cell density bioreactor , Pages 299-301 , S. Koch, H. Tanzmann, Ch. Riese, R.V. Baehr, U. Marx Cell-bubble interactions during aeration are strongly influenced by surfactants in the medium and can be minimized in the newly developed bubble bed reactor , Pages 302-304 , M. Jordan, H. Sucker, F. Widmer, H.M. Eppenberger MICRO-BUBBLE SPARGING OF ANCHORAGE DEPENDENT ANIMAL CELL CULTURES , Pages 305-308 , A. Handa-Corrigan, S. Nikolay, R. Brydges RATIONALIZATION OF THE DESIGN OF HIGH DENSITY PERFUSION CULTURES FOR SUSPENDED ANIMAL CELLS , Pages 309-311 , A. Handa-Corrigan, S. Nikolay, S. Zhang SIMULATION OF HYBRIDOMA GROWTH AND MONOCLONAL ANTIBODY PRODUCTION IN A HOMOGENOUS DIALYSIS BIOREACTOR , Page 312 , B. Szperalski, F. Geipel, T. Lorenz, U. Behrendt, J. Wahl, M. Comer Comparison of Bioreactor Technologies for Efficient Production of Recombinant α-Amidating Enzyme , Pages 315-320 , D.E. Matthews, K.E. Piparo, V.H. Burkett, C.R. Pray A COMPARISON OF ANIMAL CELL GROWTH USING MICROCARRIERS AND SUSPENDED NATURAL AGGREGATES , Pages 321-323 , P.M. Alves, J.L. Moreira, A.S. Feliciano, M.J.T. Carrondo COMPARATIVE STUDIES OF CELL PROPAGATION SYSTEMS FOR PRODUCTION OF HUMAN ACETYLCHOLINESTERASE BY RECOMBINANT CELLS , Pages 324-328 , A. Lazar, S. Reuveny, C. Kronman, B. Velan, A. Shafferman Production of a Monoclonal Antibody with a Serum Dependant Hybridoma in Different Reactor Systems , Pages 329-330 , Horst D. Blasey, Cedric O. Isch, Alain R. Bernard Transient expression with COS cells on spinner scale , Pages 331-332 , Horst D. Blasey, Alain R. Bernard PRODUCTION OF THE HIV-1 NEUTRALISING HUMAN MONOCLONAL ANTIBODY 2F5: STIRRED TANK VERSUS FLUIDIZED BED CULTURE , Pages 333-335 , M. Reiter, A. Buchacher, G. Blüml, N. Zach, W. Steinfellner, C. Schmatz, T. Gaida, A. Assadian, H. Katinger THE CYTOSENSORTM-MICROPHYSIOMETER: BIOLOGICAL APPLICATIONS OF LIVING CELLS ON SILICON SENSOR , Pages 339-344 , R. Metzger Mass spectroscopy determination of insect cell respiration rates in culture and production processes , Pages 345-350 , A.A. Kamen, R. Tom Intracellular pH evolution during batch cultures using flow cytometry , Pages 351-353 , M. Cherlet, N. Petitpain, M. Dardenne, P. Franck, J.M. Engasser, A. Marc CELL CYCLE ANALYSIS AS A TOOL FOR CONTROL AND REGULATION OF MAMMALIAN CELL CULTURES IN BIOREACTORS , Pages 354-356 , Sven M. Deutschmann, Ulrich Valley, Volker Jäger, Roland Wagner Use of MTT-assay in studies of the CHO:DG44 cell line , Pages 357-359 , Michael Hasenson On-line-measurements of metabolites in mammalian cell bioreactors by means of enzyme electrodes and of the number of living and dead cells , Pages 360-362 , U. Wellnitz, A. Rakow FULLY AUTOMATED IMAGE ANALYSIS OF CELL NUMBER, VIABILITY AND MORPHOLOGY , Pages 363-365 , K.G. Tucker, J. Welsh, M. Al-Rubeai, C.R. Thomas Fast Quantification of Mouse IgG from Cell Culture Supernatants , Pages 366-368 , Dietmar Schell On-line monitoring of growth and viral infection of VERO cells in collagen microspheres in a fluidized-bed bioreactor , Pages 369-371 , Th. Marique, D. Malarme, P. Stragier, J. Wérenne ON-LINE GLUCOSE CONTROL OF ANIMAL CELL CULTURES IN FLUIDIZED BEDS , Pages 372-375 , A.P. Loibner, N. Zach, O. Doblhoff-Dier, M. Reiter, K. Bayer, H. Katinger On-line indirect measurement of oxygen uptake rate in bench scale hybridoma cultures , Pages 376-378 , L.K. Nielsen, P.F. Greenfield Software sensors for the monitoring of high-density hybridoma perfusion cultures , Pages 379-382 , F. PELLETIER, C. FONTEIX, A. LOURENÇO da SILVA, J.L. GOERGEN, A. MARC, J.M. ENGASSER Application of an industrial disc stack centrifuge for the separation of hybridoma cells as a first step in downstream processing , Pages 383-385 , R. Kempken, A. Preißmann, J. Schäfer, W. Berthold DYNAMIC INTERFACIAL TENSION AND RHEOLOGICAL PROPERTIES OF CELL CULTURE MEDIUM WITH SHEAR PROTECTANT ADDITIVES , Pages 389-391 , James Michaels, Ameet K. Mallik, Jason E. Nowak, Darsh T. Wasan, E.T. Papoutsakis MIXING PHENOMENA IN INDUSTRIAL BIOREACTORS WITH PERFUSION SPIN FILTERS , Pages 392-396 , K. Jim Jem, Seif Fateen, James Michaels HYDRODYNAMIC CONTROL OF SUSPENDED NATURAL ANIMAL CELL AGGREGATES , Pages 397-401 , J.L. Moreira, A.S. Feliciano, P.M. Alves, J.G. Aunins, M.J.T. Carrondo MODELLING AND PREDICTION OF ANIMAL CELL DISRUPTION IN SIMPLE FLOWS , Pages 402-404 , Z. Zhang, C. Born, M. Al-Rubeai, C.R. Thomas BIOSYNTHETIC RESPONSES AND SCANNING ELECTRON MICROSCOPIC STUDIES OF MURINE HYBRIDOMAS SUBJECTED TO HYDRODYNAMIC STRESSES , Pages 405-409 , Steve K.W. Oh, Mohamed Al-Rubeai, Nick Emery, Alvin Nienow Fed-batch Strategies for Mammalian Cell Cultures , Pages 413-418 , W. Noe, P. Schorn, R. Bux, W. Berthold GROWTH AND PRODUCTIVITY OF ANIMAL CELLS: A CONTRIBUTION TO SOME FACTORS INFLUENCING BOTH , Pages 419-421 , G. Kretzmer, A. Ludwig, R. Weidemann, M. Teige, J. Tomeczkowski Simultaneous Control of Growth and Productivity Using a Mutant CHO Cell Line , Pages 422-424 , Alison Hovey, Chris Bebbington, Nigel Jenkins FACTORS AFFECTING GROWTH, INFECTION AND PROTEIN YIELD IN BACULOVIRUS -INFECTED INSECT CELLS , Pages 425-427 , N. Kioukia, M. Al-Rubeai, A.W. Nienow, A.N. Emery Effects of the feed medium glucose concentration on growth and metabolism of hybridomas in chemostat culture , Pages 428-430 , J.J. Meijer, J.P. van Dijken, K.Ch.A.M. Luyben Effects of Glucose on the Production of Recombinant Protein C in Mammalian Cell Culture , Pages 431-433 , Takeyuki Sugiura Substrate level control in fed-batch hybridoma cultures , Pages 434-436 , B. Romein, P. van Londen, J.J. Meijer, C. Hellinga, J.P. van Dijken, K.Ch.A.M Luyben Effect of Dissolved Oxygen on Monoclonal Antibody Production from Hybridoma Cultured in Haemodialysers , Pages 437-456 , G.B. Ryan, M.T. Simpson, W.T. Jones, M.J. Nicol, P.H.S. Reynolds Metabolic parameters of a hybridoma cell line in batch and continuous cultivation , Pages 457-459 , Ralf Pörtner, Armin Bohmann, Ines Lüdemann, Herbert Märkl Overproduction Of Monoclonal Antibodies In High Salt Medium In The Presence of Butyrate , Pages 460-464 , Steve K.W. Oh, Pooja Vig, Florence Chua, W.K. Teo, Miranda G.S. Yap GROWTH CONTROL OF PRODUCTION PROCESSES WITH BHK CELLS AND REGULATION OF THE PERFUSION RATE BY MONITORING THE INTRACELLULAR NUCLEOTIDE POOLS , Pages 465-469 , Ulrich Valley, Thomas Ryll, Roland Wagner Control of the maximal cell density in a membrane perfusion reactor , Pages 470-475 , H. PINTON, A. LOURENCO da SILVA, J.L. GOERGEN, A. MARC, J.M. ENGASSER, J.N. RABAUD, G. PIERRY Continuous Medium Recycling in Pilot Scale with a Recombinant Animal Cell Culture for Antithrombin III Production , Pages 476-480 , U. Riese, D. Lutkemeyer, R. Heidemann, H. Büntemeyer, J. Lehmann OPTIMIZATION OF ANTIBODY PRODUCTION IN A FLUIDIZED BED BIOREACTOR , Pages 481-484 , G. Rolef, M. Biselli, R. Dunker, C. Wandrey MONOCLONAL ANTIBODIES RELEASED FROM VIABLE HYBRIDOMA CELLS AT DIFFERENT STAGES OF GROWTH , Pages 485-493 , H. Musielski, K. Rüger, M. Zwanzig, K. Lehmann HIGH CELL DENSITY IN INSECT CELL CULTURES , Pages 494-503 , S. Reuveny, C.W. Kemp, J. Shiloach Semi-continuous production with the baculovirus expression system , Pages 504-506 , Frank L.J. van Lier, Menno M. Engelkes, Leo A. van der Pol, Cornelis D. de Gooijer, Johannes Tramper LONG TERM CULTURES OF BHK SUSPENDED AGGREGATES , Pages 507-510 , J.L. Moreira, A.S. Feliciano, J.G. Aunins, M.J.T. Carrondo Batch Kinetic Data of Hybridoma Growth and Productivity as a Basis for Simulation of Antibody Production in Different Culture Systems , Pages 513-517 , Jörg Thömmes, Manfred Biselli, Maria-Regina Kula, Christian Wandrey Modelling insect cell cultures infected with recombinant baculovirus , Pages 518-520 , L.K. Nielsen, J. Power, K. Radford, S. Reid, P.F. Greenfield KINETIC ANALYSIS OF HYBRIDOMA GROWTH AND MONOCLONAL ANTIBODY PRODUCTION IN SEMICONTINUOUS AND CONTINUOUS CULTURES WITH RESPECT TO BATCH CULTURES , Pages 521-531 , O-W MERTEN, D MOEURS, H KELLER, M LENO, L CABANIE, E COUVE PROCESS DEVELOPMENT AND KINETIC ANALYSIS OF A PROTEIN-FREE HYBRIDOMA PERFUSION SYSTEM , Pages 532-538 , Sylvain Mercille, Mark Johnson, Lucie Bourget, Réal Lemieux, Bernard Massie Steady-state analysis of hybridomas in chemostat culture , Pages 539-541 , J.J. Meijer, B. Romein, J.P. van Dijken, K.Ch.A.M. Luyben KINETIC STUDIES OF FED-BATCH HYBRIDOMA CULTURES: EFFECT OF VARIOUS FEEDING COMPOSITIONS AND FLOW RATES , Pages 542-544 , M. DARDENNE, M. CHERLET, J.M. ENGASSER, A. MARC A general two-step procedure for the kinetic modelling of animal cell cultures , Pages 545-547 , V. Chotteau, G. Bastin Mathematical Modeling of the Growth and Production Kinetics in Hybridoma Cultures , Pages 548-550 , R.K. Biener, R. King, M. Howaldt, W. Noé, E.D. Gilles Impact of Improved Chromatographic Media on Productivity and Process Design in Downstream Processing , Pages 553-556 , Carina Schmidt, Jan H. Berglöf, Lars-Olof Lindquist Direct Capture of Recombinant Proteins from Animal Cell Culture using a Fluidized Bed , Pages 557-562 , John C. Erickson, John D. Finch, David C. Greene Chromatographic Separation of Immunoglobulins on a New “Tentacle”-type “TA”-Phase: Fractogel EMD TA 650(S) , Pages 563-566 , W. Brümmer, E. Müller, W. Müller, E. Merck VALIDATION OF CHROMATOGRAPHIC PROCEDURES USED IN THE FRACTIONATION OF PLASMA DERIVATIVES , Pages 567-570 , A.J. Darling, U.Ch.K. Kreuzburg-Duffy, L. Borland, A.E. Morrow, J.H. Berglöf, I. Andersson Successful products and future business prospects , Pages 573-579 , Rolf G. Werner PRODUCTION OF RECOMBINANT FACTOR VIII FROM PERFUSION CULTURES: I. LARGE-SCALE FERMENTATION , Pages 580-583 , B.G.D. Bödeker, R. Newcomb, P. Yuan, A. Braufman, W. Kelsey PRODUCTION OF RECOMBINANT FACTOR VIII FROM PERFUSION CULTURES: II. LARGE-SCALE PURIFICATION , Pages 584-590 , B.G.D. Bödeker, E. Potere, G. Dove ADVANTAGES OF HIGH CELL DENSITY IN VITRO CULTURES IN DOWN STREAM PROCESSING OF A MONOCLONAL IgG ANTIBODY FOR IN VIVO USE , Pages 591-593 , P. Preikschat, U. Marx, R.v. Baehr Production, purification and application of flatfish interferon , Pages 594-602 , Hiroki Murakami, Tadakazu Tamai, Tohru Noguchi, Nobuyuki Sato, Shouji Kimura, Sanetaka Shirahata Monoclonal Antibody Production srategy for serum dependent clone , Pages 603-604 , Cedric Isch, Alain R. Bernard, Horst D. Blasey HIGH LEVEL MEASLES VIRUS N PROTEIN EXPRESSION USING A RECOMBINANT BACULOVIRUS: ASSEMBLY OF NUCLEOCAPSID-LIKE STRUCTURES , Pages 605-609 , Anthony R. Fooks, John R. Stephenson, Alan Warnes, Barry Dowsett, Gavin W. Wilkinson, Bert K. Rima EQUIMOLAR EXPRESSION OF TWO PROTEIN CHAINS IN RECOMBINANT MAMMALIAN CELLS , Pages 610-616 , Wilhelm Dirks, Hansjörg Hauser Expression of Mammalian Protein Farnesyltransferase in a Baculovirus System , Pages 617-618 , John F. Moomaw, Patrick J. Casey, Wen-Ji Chen, Laurie Overton, Thomas A. Kost LEVELS OF TROPONIN I AND MYOSIN HEAVY CHAIN IN DEDIFFERENTIATED ADULT RABBIT CARDIAC MYOCYTES , Pages 619-621 , I. McPhee, K.A. Kane, C. MacDonald EXPRESSION OF THE D 2 AND D 3 DOPAMINE RECEPTORS IN INSECT CELLS USING THE BACULOVIRUS SYSTEM , Pages 622-624 , C. Woodcock, P.G. Strange, B.C. Rooney PRODUCTION AND CHARACTERIZATION OF SOLUBLE MOUSE AND HUMAN INTERFERON-γ RECEPTORS (IFNγ-R) FROM INSECT CELLS , Pages 625-632 , Georg Schmid, Nathalie Wild, Michael Fountoulakis, Harald Gallati, Reiner Gentz, Laurence Ozmen, Gianni Garotta, F. Hoffmann-La Generation of DNA-packaging proteins by overexpression in the Baculovirus/insect cell system , Pages 633-637 , V. Sandig, J. Forstova, N. Krauzewicz, B.E. Griffin, M. Strauss Metalloproteinases and TIMPs production by AIDS-Kaposi sarcoma derived cells , Pages 638-642 , D. Blankaert, D. Parent, C.M. Farber, J.P. van Vooren, C. Liesnard, J. Wérenne Endothelial cells and their infection with the rickettsia Cowdria ruminantium : prospects for vaccine production , Pages 643-645 , Ph. Totté, D. Blankaert, T. Marique, N. Vachiery, Guerra I. Teixeira, J. Werenne, C. Kirkpatrick, J.P. Vanvooren CHANGES IN THE GLYCOSYLATION PATTERN OF RECOMBINANT PROTEINS EFFECTED BY DEFINED CULTURE CONDITIONS OF BHK-21 CELLS , Pages 649-651 , Martin Gawlitzek, Roland Wagner, Harald S. Conradt, Corinne Villers, André Verbert Glycosylation analysis of a human monoclonal IgG antibody derived from a human- mouse heterohybridoma , Pages 652-657 , H. Leibiger, A. Hansen, H. Böhme, U. Marx Culture pH and ammonia affect expression rates and glycosylation of recombinant mouse placental lactogen proteins by CHO cells , Pages 658-660 , E.T. Papoutsakis, D.I.H. Linzer, M.C. Borys Analysis of the product consistency: Independent of process parameters, rt-PA shows a stable glycosylation pattern , Pages 661-666 , Kristina Kopp, Wolfgang Noé, Michael Schlüter, Franz Walz, Rolf Werner GALACTOSYLATION OF HUMAN/MOUSE IGG ANTI–NP ANTIBODIES AND ITS DETECTION BY ELISA , Pages 667-669 , M. Goodall, T. Bentley, J. Lund, R. Jefferis, N. Takahashi Characterization of carbohydrate chain on light chain of human monoclonal antibody , Pages 670-675 , Hiroki Murakami, Hirofumi Tachibana Comparison of the posttranslational modifications of human proteins secreted from different recombinant baculovirus infected insect cell lines , Pages 676-678 , Eckart Grabenhorst, Albrecht Gröner, Volker Jäger, Marcus Ackermann, Christine Karger, Kathrin Rollwage, Manfred Nimtz, Harald S. Conradt CHARACTERIZATION OF INTRA- AND EXTRACELLULAR PROTEASES IN RECOMBINANT MAMMALIAN AND HYBRIDOMA CELLS , Pages 679-682 , Ricardo B. Kratje, Waldemar Lind, Roland Wagner IMPACT OF FACTORS SECRETED BY MAMMALIAN AND INSECT CELLS ON HUMAN MONOCLONAL ANTIBODY INTEGRITY , Pages 683-687 , Marcus Ackermann, Volker Jäger, Uwe Marx BiP - a chaperone involved in antibody formation , Pages 688-693 , INGRID G. HAAS, CORNELIA R. KALOFF, MICHAEL R. KNITTLER Cell systems for in vitro toxicology , Pages 697-710 , Herbert Georg Miltenburger HEPATOCYTES: in vitro PHARMACOLOGY, TOXICOLOGY AND DRUG METABOLISM , Pages 711-722 , J.V. Castell, M.J. Gómez-Lechón, M.T. Donato Development of an Optimised in vitro System for Measurement of Human Tumour Response to Cytotoxic Agents , Pages 723-728 , C. Mothersill, M. Sheridan, J. Harney, J. Bonnar, T.P. Hennessy, C.B. Seymour Threedimensional cell cultures for toxicity testing and for the estimation of the affinity of cells to biocompatible materials? , Pages 729-731 , U. Wellnitz, A. Rakow Stable Expression of the Bovine Adrenal 11ß-Hydroxylase, a Cytochrome P 450 Enzyme, in CHO Cells as Tool for Drug Characterization , Pages 732-734 , Thomas Petri, Manfred Husemann ACTIVATION AND EXPANSION OF HUNAN CYTOTOXIC T LYMPHOCYTES IN HOLLOW FIBER BIOREACTORS , Pages 735-737 , C.H.J. Lamers, R.J. van de Griend, J.W. Gratama, R.L.H. Bolhuis Significance of contamination with viruses of cell lines used in the production of biological medicinal products , Pages 741-750 , P D Minor RESIDUAL IN VITRO ACTIVITY OF DNA IN BIOLOGICALS TREATED BY BETA-PROPIOLACTONE: RABIES VACCINES AS MODEL , Pages 751-753 , S. Morgeaux, N. Tordo, O. Merten, P. Perrin The development of appropriate viral models for the validation of viral inactivation procedures , Pages 754-756 , C.Z. Tao, R. Cameron, C. Harbour, J.P. Barford REGULATORY AFFAIRS SAFETY TESTING FOR GENE THERAPY PRODUCTS , Pages 757-759 , Exeen Morgan, Jeffrey Ostrove PRODUCT QUALITY STUDIES OF ANTITHROMBIN III DURING MEDIUM RECYCLING IN PILOT SCALE , Pages 760-762 , D. Lütkemeyer, U. Riese, R. Heidemann, M. Bretschneider, J. Lehmann, H.S. Conradt Product Consistency During Long-Term Fed-Batch Culture , Pages 763-768 , D.K. Robinson, C.P. Chan, T.C. Seamans, D.K. Lee, A.B. Lenny, J.-S. Tung, D.J. DiStefano, S. Munshi, S.L. Gould, G.E. Mark, C.C. Yu Ip, P.K. Tsai, J. Irwin, M. Silberklang THE APPLICATION OF INSECT CELLS FOR BIOPHARMACEUTICAL PRODUCTION: IMPLICATIONS FOR SAFETY TESTING , Pages 769-774 , C. McLean, A.J. Shepherd Generation of Linear Epitope Specific Antibodies Against the Human Recombinant Tissue-Plasminogen-Activator (rt-PA) , Pages 775-778 , Frank Hanakam, Günther Jung, Wolfgang Berthold, Rolf G. Werner, William Werz List of Participants , Pages 779-815 AUTHOR INDEX , Pages 816-819 Index , Pages 820-830