Animal cell technology : products from cells, cells as products : proceedings of the 16th ESACT Meeting : April 25-29, 1999, Lugano, Switzerland

this Book Contains, In The Form Of Concise Papers Of Limited Length, The Proceedings Of The 16th Esact Meeting That Was Held In Lugano, Switzerland, In April 1999. We Hope It Will Become A Useful Resource Of The Most Up-to-date Information In Animal Cell Technology, At Least Until The Next Meeting In 2001. Classical Approaches For The Use Of Animal Cells (for Example, Production Of Virus Vaccines) Remain An Important Technology. However, It Appears That Major Technological Advances And Major Growth Are Occurring In Other Areas. Most Importantly, Protein Production On The Basis Of Recombinant Dna Molecules Transferred Into Animal Cells Appears To Be An Ever-increasing Field Of Interest And Innovation. Increasingly Animal Cells Are Being Used As Substrates For The Study Of Gene Activation And Repression, And Also For The More Rapid Production Of Small And Moderate Quantities Of Interesting Proteins. Tissue Engineering, Somatic Gene/cell Therapy, Organ-replacement Technologies, And Cell-based Bio-sensors All Contribute To A Considerable Widening Of Interest And Research Activity, Based On Animal Cell Technology. Front Matter....Pages 1-1 Recombinant Pharmaceutical Protein Overexpression in an IRF-1 Proliferation Controlled Production System....Pages 3-9 The Real Meaning of High Expression ....Pages 11-17 A Novel Mammalian Gene Regulation System Responsive to Streptogramin Antibiotics....Pages 19-22 Feedback Control of Redox Potential in Hybridoma Cell Culture....Pages 23-29 Genetic Manipulation of the Protein Synthetic Capacity of Mammalian Cells....Pages 31-33 Retinoic Acid Enhances Monoclonal Antibody Production of Human-Human Hybridoma BD9....Pages 35-37 Extracellular Adenosine 5’ Monophosphate Controls Proliferation and Stimulates Protein Production of rCHO Cells....Pages 39-41 The Mode of Actions of Lysozyme as an Immunoglobul in Production Stimulating Factor (IPSF)....Pages 43-45 A Dissection of Recombinant Protein Expression in CHO Cells Indicating Protein-Specific, not Cells Specific, Limitation....Pages 47-49 Effect of Bioreactor Process Control Parameters on Apoptosis and Monoclonal Antibody Production During the Protein-Free Fed-Batch Culture of a Murine Myeloma....Pages 51-53 Adenovirus Vector Production in 293 Fed-Batch Cultyres....Pages 55-57 Influence of BCL-2 Over-Expression on NS0 and CHO Culture Viability and Chimeric Antibody Productivity....Pages 59-61 A Regulatable Selective System Facilitating Isolation of High Expression Mammalian Cells....Pages 63-65 Comparative Analysis of IRES Efficiency of Dicistronic Expression Vectors in Primary Cells and Permanent Cell Line....Pages 67-69 Use of Gel Microdrops Selection System for Recombinant Cells....Pages 71-73 Bovine Serum-Albumin with Grafted RGD Tailed Cyclic Peptudes as an Engineered Proadhesive Protein....Pages 75-77 Matrix Attachment Regions in Stable CHO Cell Line Development....Pages 79-81 High Cell Density Cultivation of Hybridoma Cells: Spin Filter vs Immobilized Culture....Pages 83-85 Influence of the Inoculum on the Production of Antibodies with Mammalian Cells....Pages 87-89 Establishing and Developing Cho Cell Lines for the Commercial Productoin of Human Anti-Rhesus D IgG....Pages 91-93 Front Matter....Pages 1-1 Glucose and Glutamine Replacement for the Enhancement of Fed-Batch Processes Using CHO Cells....Pages 95-97 Pilot Scale Production of Human Prothrombin Using a Recombinant CHO Cell Line....Pages 99-101 Maintenance of High Viability During Direct Transition of CHO-K1 Cell Growth from 10% to 0% Serum; No Adaptation Required....Pages 103-106 Analysis and Simultaneous Isolation of Recombinant t-PA Using High Performance Membrane Affinity Chromatography....Pages 107-109 Front Matter....Pages 111-111 Improvement of the Primary Metabolism of Cell Cultures Introducing a Pyruvate Carboxylase Reaction Pathway....Pages 113-117 Growth Kinetic Studies (In Hours) Based on the Fluorescence of Stable, GFP-Expressing CHO Cells....Pages 119-121 Intracellular Localization of Non-Secreted Recombinant β-Trace Protein in Vesicle-Like Structures of Baculovirus-Infected Insect Cells....Pages 123-125 Induction Kinetics of Apoptosis in Mammalian Cell Cultures....Pages 127-129 A Model for the On-Line Scrutiny of Metabolism: Its Application to the Changing Nutritional Demands of Cultured Animal Cells....Pages 131-133 Caspases as a Target for Apoptosis Inhibition in Hybridoma Cells in Culture....Pages 135-137 Cell Activity After Cell Growth Inhibition by the IRF-1 System....Pages 139-141 Establishment of a Pramary Liver Cell Culture from a Teleost, Oreochromis Mossambicus , the Tilapia: a Valid Tool for Physiological Studies....Pages 143-145 TGF-β Induces Premature and Replicative Senescence in Cancer Cells....Pages 147-149 Development of a Fed-batch Strategy That Utilizes Multiple Carbon Sources to Maximize Recombinant Protein Eepression in CHO Cells....Pages 151-153 Development of a New, Highly Flexible, Fully Computer Integrated Fermentor Control System....Pages 155-157 Cell Growth Rate Estimation in Packed-Bed and Hollow Fibers Bopreactprs: Metabolism and Productivity....Pages 159-161 Influence of Oxygen Limitation on Myeloma Cell Cultures....Pages 163-165 Comparison of Fed-batch Strategies in Hybridoma Cultures....Pages 167-169 Non-Invasive Adaptive Control of the Feed Rate for High Cell Density CHO Cultures According to the pO2 Profile....Pages 171-173 Cell Growth Control by the IRF-1 System in Perfusion Culture....Pages 175-177 Front Matter....Pages 111-111 Modulation of Cell Cycle for Optimal Recombinant Protein Production....Pages 179-181 Application of Bioassays for Suspended 293 Cell Enumerations in Chemically Defined Medium (CDM)....Pages 183-186 Efficient Cell Growth by an Optimised Protein Free Medium Design and a Controlled Feeding Strategy....Pages 187-189 Development of a Serum Free Meditum for a Human Diploid Fibroblast Cell Line....Pages 191-193 The Use of Peptones as Medium Additives for High-Density Perfusion Cultures of Animal Cells....Pages 195-197 Determination of Animal Cell Densities and Viabilities by the New Analyser CEDEX2....Pages 199-201 Determination of Cell Numbers and Viability; Comparison of the Microcyte Flow Cytometer with Trypan Blue Counts and Coulter Counts....Pages 203-205 Mid-Infrared Spectroscopic Monitoring of Animal Cell Culture Bioreactor Processes....Pages 207-209 Mammalian Cell Monitoring Using the Microcyte Flow Cytometer....Pages 211-213 Indirect Biomass Determination in Case of Con-Constant Metabolic Rates....Pages 215-217 Whole Cell-Smart Microsensor: A first Approach Towards Interfacing Live Cells and Electronics....Pages 219-221 Comparison of Immobilised Group Specific Affinity Ligands for the Bioseparation of Antibodies by High Performance Membrane Affinity Chromatography....Pages 223-225 Front Matter....Pages 227-227 Posttranslational Modifications Limit High Level Expression of Functionally Active Chimeric P-Selectin Glycoprotein Ligand-1 in rCHO Cells....Pages 229-235 Glycosylation Analysis of Nanomolar Amounts of Glycoprotein Combining In-gel Enzymatic Digestion and FACE TM ....Pages 237-239 Glycosylation Patterns of a rec-Fusion Protein Expressed in BHK Cells at Different Metabolic States....Pages 241-243 Elimination of N-Glycolylneuraminic Acid Residues in Recombinant Glycoproteins: A Gene Knock-Out Approach....Pages 245-249 Fucosyltransferase III Production by Mammalian and Insect Cells for the Synthesis of Therapeutic Oligosaccharides....Pages 251-253 Control of Therapeutic Monoclonal Antibody Glycosylation....Pages 255-257 The Effect of Cell Line, Transfection Procedure and Reactor Conditions on the Glycosylation of Recombinant Human Anti-Rhesus D IgGl....Pages 259-261 Metabolic Engineering of Recombinant Plasminogen Sialylation in Animal Cells by Tetracycline-Regulated Expression of Murine CMP-NeuAc Synthetase and Hamster CMP-NeuAc Transporter Genes....Pages 263-265 Front Matter....Pages 267-267 Air-Trap-Technology: Bubble-Free Gas Transfer in Large Scale....Pages 269-275 The Improvement of Aeration in 8000 I Animal Cell Culture Vessels....Pages 277-283 A Novel Continuous Suspension Culture System for Hematopoietic Cells....Pages 285-287 Large Scale Production of HIV-I gp120 Using Recombinant Vaccinia Virus in Packed-Bed Bioreactor....Pages 289-291 The “Shear Tester”....Pages 293-297 Membrane Oxygenation of Mammalian Cell Culture Fermenters Using Dupont Teflon AF-2400 Tubing....Pages 299-301 Configuration of a Simple Dialysis Membrane Bioreactor Suitable for Large-Scale Application....Pages 303-305 Erythropoietin Production from CHO Cells Grown in a Fluidized-Bed Bioreactor with Macroporous Beads....Pages 307-309 Scale-Up of Fixed-Bed Reactors for the Cultivation of Animal Cells....Pages 311-313 A Novel Conical Shaped Bioreactor for Small-Scale Investigations Under Defibed Conditions....Pages 315-317 Continuous Perfused Fluidized Bed Technology....Pages 319-321 Small Scale Bioreactor System for Process Development and Optimization....Pages 323-327 Front Matter....Pages 329-329 Mammalian Transient Gene Expression-Potential, Practical Application and Perspectives....Pages 331-337 Float! — And Express More....Pages 339-345 Multiplicity and Time of Infection as Tools to Maximize Virus-Like Particle Production by Insect Cells....Pages 347-349 Process Development for Transient Gene Expression in Mammalian Cells at The 3 L Scale: 10–50 MG of R-Protein in Days....Pages 351-357 A Method to Determine the Optimal Time to Infect Insect Cells with the Baculovirus Expression System....Pages 359-361 Analysis of Baculovirus Infected Sf9 Cells Using Flow Cytometry....Pages 363-365 Adaptation of Recombinant HEK-293 Cells to Growth in Serum Free Suspension....Pages 367-369 Growth on Microcarriers and Nutritional Needs of High Density Insect Cell Cultures....Pages 371-373 Front Matter....Pages 329-329 Use of Flow Cytometry in the Evaluation of Hydroxyapatite for the Purification of Plasmid DNA....Pages 375-377 Front Matter....Pages 379-379 Regulation and Standardi Sation of Gene Transfer Products in the European Union....Pages 381-387 Study of Stability of Expression of a Humanized Monoclonal Antibody from a Transfected NSO Myeloma Cell Line Using Different Culture Media and Serum Concentrations....Pages 389-391 Analysis of Alterations in Gene Expression after Amplification of Recombinant Genes in CHO Cells....Pages 393-395 Stable Recombinant Expression and Functinal Identity of the Anti HIV-I Monoclonal Antibody 2F5 after IGG3/IGGI Subclass Switch in CHO-Cells....Pages 397-399 Analysis of Genomic DNA and RNA-Transcripts of a Heterohybridoma SDNA imultanously Expressing IgG3 and IgG1 with Identical Specificity....Pages 401-403 Front Matter....Pages 405-405 Tissue Engineering of a Bladder Wallpatch....Pages 407-411 Integrated Optimization of Retrovirus Production for Gene Therapy....Pages 413-420 Toxicological Evaluation of Surface Water Samples in Sensitized Cultured Fish Cells, and Comparison with the Microtox 00AE; Method....Pages 421-423 Electrolyzed and Natural Reduced Water Exhibit Insulin-Like Activity on Glucose Uptake into Muscle Cells and Adipocytes....Pages 425-427 Study of DDS for a Cancer Therapy Applying Lipid Vesicle Immobilizing Eucheuma Serra Lectin....Pages 429-431 Study of the Specific Binding between Lipid Vesicles and Human-Human Hybridoma toward Eitger DDS or Gene Transfection....Pages 433-435 Suppression of Apoptosis in UV-Damaged Human Melanomacells by a Fermented Milk, Kefir....Pages 437-439 Large Scale GMP Production of Retroviral Gene Therapy Vectors in Vert Low Serum Medium....Pages 441-443 Front Matter....Pages 445-445 Chimeric Lyssavirus Glycoprotein: New Vector for Multivalent Vaccines....Pages 447-453 The SPP Biotech Module 1: Proteins for Medical Applications a Multidisciplinary Effort for the Development of a Recombinant Anti-RhD Antibody Preparation of Hemolytic Disease of the Newborn....Pages 455-458 Low Serum and Serum-Free Cultivation of Mammalian Cells Used for Virus Production Applications....Pages 459-461 Development of a Serum-Free Medium for MRC-5 Culture....Pages 463-465 Adaptation of Vero Cells to a Serum Free Medium for the Production of Rabies Virus....Pages 467-469 Increased Productivity of a Rickettsia Vaccine: Enhancement of Adhesion Properties of Infected Endhothelial Cells by TGFβ....Pages 471-473 Front Matter....Pages 445-445 Comparative Study of Cell Cultures Perspective for Rubella Virus Production....Pages 475-477 Biogenesis of Epithelial Phenotype During Long-Term Cultivation of MDCK Cells in Serum-Free Ultra-MDCK Medium....Pages 479-481 Sandwich Elisa Today: Limits for Sensitivity, Speed, Precision and Throughput....Pages 483-485 Front Matter....Pages 487-487 Impact of ICH Guidelines on Phases of Development of Biotechnology Products....Pages 489-493 The Microbial Risks of Xenotransplantation....Pages 495-499 Cryopreservation under Protein-Free Medium Conditions: A Reliable Way to Safe Cell Banking....Pages 501-503 Creation of Certified Cell Banks Suitable for Production of Healing and Immunobiological Preparations....Pages 505-507 Endogeneous Retrovirus Particles and Their Eeffcts on the Growth Behavior of Hybridoma Cultivation Processes....Pages 509-511 Testing for Adventitious Retroviruses Using PCR-Based Revese Transcriptase Assays....Pages 513-515 Rapid RNA Quantitation from Xenotropic SP2/0 Retroviruses by Competitive RT-PCR ELISA....Pages 517-519 Human Amniotic Epithelial (HAE) Cells Express Erythropoietin (EPO) and EPO-Receptor....Pages 521-523 The Nordenau-Phenomenon—Facts and Hypotheses....Pages 525-527 Conversion of a Process Development Pilot Plant into a Clinical Phase I/II Manufacturing Facility for Recombinant Proteins Expressed in Mammalian Cells....Pages 529-531 Front Matter....Pages 533-533 Human Keratinocyte Culture on Macroporous Carries at the Air-Liquid Interface....Pages 535-537 Comparison of Viability and Function of Primary Cells with Transformed Cellsfor use in a Bioartificial Liver Support System....Pages 539-541 Determining Optimal Transport Conditions of In Vitro Cultured Human Chondrocytes Prepared for Autologous Re-Implantation Using Flow Cytometry....Pages 543-545 Effects of Collagenase on Human Hematopoietic Cells....Pages 547-549 Validation of the CACO-2 Test for Intestinal Permeability Assessment of Drugs: The Usefulness of PAPP, the Apparent Permeability Coefficient....Pages 551-553