Animal cell technology : challenges for the 21st century : proceedings of the Joint International Meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT), 1998, Kyoto, Japan

animal Cell Technology Is A Growing Discipline Of Cell Biology Which Aims Not Only To Understand Structures, Functions And Behaviors Of Differentiated Animal Cells But Also To Ascertain Their Abilities To Be Used In Industrial And Medical Purposes. The Goal Of Animal Cell Technology Includes Accomplishments Of Clonal Expansion Of Differentiated Cells With Useful Ability, Optimization Of Their Culture Conditions, Modulation Of Their Ability To Produce Medically And Pharmaceutically, Important Proteins, And The Application Of Animal Cells To Gene Therapy And Artificial Organs. This Volume Gives The Readers A Complete Review Of Present State Of The Art In Japan. The Proceedings Will Be Useful For Cell Biologists, Biochemists, Molecular Biologists, Immunologists, Biochemical Engineers And Other Disciplines Related To Animal Cell Culture, Working In Either Academic Environments Or In Biotechnology And Pharmaceutical Industries. The Role of Hong Kong SAR in the Development of Biotechnology Industry in China....Pages 1-4 Increase in Intracellular Calcium Influx Enhances Monoclonal Antibody Productivity....Pages 5-9 Energy Metabolism and Antibody Production Based on Flux Analysis in Hybridoma....Pages 11-15 Transient Transfection Induces Early Cytosolic Calcium Signaling in CHO-K1 Cells....Pages 17-22 An On-Line Feeding Strategy for Fed-Batch and Dialysis Cultures of Hybridoma Cells....Pages 23-27 Effects of Serum and Other Medium Components on Suspension Culture of CHO Cells Producing Tissue Plasminogen Activator....Pages 29-32 Effects of Osmotic Pressure on Metabolism of CHO Cells Producing Tissue Plasminogen Activator in Adhesion Culture Compared with that in Suspension Culture....Pages 33-38 Cultivation of CHO Cells on Novel Microcarriers....Pages 39-43 Bleeding Strategy for the Long-Term Perfusion Culture of Hybridoma....Pages 45-49 Metabolic Flux Distributions in Hybridoma Cells at Different Metabolic States....Pages 51-55 On-Line Detection of Metabolic Demand and Changes to it: Heat Flux in Continuous Cultures of Animal Cells....Pages 57-61 Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Culture....Pages 63-67 A Kinetic Model of Macromolecular Metabolism in Batch and Fed-Batch Cultures of Hybridoma Cells Producing Monoclonal Antibody....Pages 69-73 Ehancement of Antibody Productivity in the Near-Zero Specific Growth Rate During Glucose and Glutamine-Limited Fed-Batch Culture of Hybridoma Cells....Pages 75-79 Long-Term Culture of CHO Cells on Porous Microcarriers in a Stirred Tank Equipped with a Modified Cell Retention System....Pages 81-85 Continuous production and recovery of recombinant Ca ++ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor....Pages 87-91 Characterization of Recombinant Bovine Lactoperoxidase Produced by CHO Cells....Pages 93-97 Expression of a Soluble αβ Heterodimeric T Cell Receptor in Animal Cells....Pages 99-103 Posttranslational Modifications of Recombinant Von Willebrand Factor: Limitations and Experimental Improvement at High Yield Expression....Pages 105-109 Incubation with Heparin as an Improved Harvest Method for High Titre Disabled Infectious Single Cycle (DISC) HSV-2 from Microcarrier Cultures....Pages 111-115 Passaging of Microcarrier Cultures as an Alternative Method for the Seeding of Large Scale Bioreactors for the Production of High Titre Disabled Infectious Single Cycle HSV-2 Vaccine (DISC HSV)....Pages 117-121 The Hybrid System Using Both Promoter Activation and Gene Amplification for Establishment of Exogenous Protein Hyper-Producing Cell Lines....Pages 123-127 System for Large Scale Protein Expression in Bombyx mori ....Pages 129-132 Protein-Free Culture of Ras -Amplified Recombinant BHK-21 Cells....Pages 133-137 Effect of Sugar Composition on the Heterogeneity of Antibod in Hybridoma Cultivation....Pages 139-143 Modulation of Antigen Bunding of Human Antibody Via Glycosylation by Hybridoma Culture with Various Carbon Sources....Pages 145-148 Manipulation of Cytokine Production from CD8 + T Cells by Means of Amino Acid-Substituted Analogs of a Peptide Antigen....Pages 149-153 A Novel Effect of Nivalenol (NIV) on the Immune Response....Pages 155-160 Human-Human Hybridomas Producing Monoclonal Antibodies Reactive to House Dust Mites Antigen....Pages 161-165 Establishment of Human-Mouse Hybridomas Secreting Antibodies to Rice-Allergens....Pages 167-170 Cell Culture Engineering Using Intelligent Biomaterials....Pages 171-175 Spatial Development of Mouse Bone Marrow Cells Cultivated in Porous Carriers....Pages 177-180 Tumor Promoting Mechanism of Biomaterials....Pages 181-185 Xenotransplantation of Cultured Newborn Pig Thyroid Tissue for the Treatment of Post-Radioiodine Hypothyroidism In Rats....Pages 187-191 Improved Production of Retroviral Vectors Under Serum-Free Conditions for the Application of Gene Therapy....Pages 193-197 Large Scale Production of Rh-Albumin Expressed in the Milk of Transgenic Cattle — An Economic and Technical Challenge....Pages 199-202 Transgenic Rabbits: A Novel Model for the Study of Atherosclerosis....Pages 203-207 In Vivo Gene Transfer to Chicken Via Blastodermal Cells of Early Developmental Embryos....Pages 209-213 Overview of the International Endeavor toward Harmonization of Technical Requirements for the Control of New Medicines from Biotechnology....Pages 215-219 Reducing Risks of Animal Origin Contaminants in Cell Culture....Pages 221-225 Cytotoxicity Testing for Evaluating Food Safety....Pages 227-229 Factors Specifically Expressed in Osteoblasts....Pages 231-241 Osteoclast Differentiation Factor is a Ligand for Osteoprotegerin / Osteoclastogenesis-Inhibitory Factor....Pages 243-247 Erythropoietin Protects Neurons From Ischemic Damage....Pages 249-253 Monovalent Antigen Activates Antibody/Cytokine Receptor Chimera and Controls Hematopoietic Cell Growth....Pages 255-259 Molecular Cloning of the Genes that Determine the Invasive Activities of Rat Ascites Hepatoma Cells....Pages 261-264 Antitumor Protein (AP) from a Mushroom Induced Apoptosis to Transformed Human Keratinocyte by Controlling the Status of pRB, c-MYC, Cyclin E-CDK2, and p21 WAF1 in the GI/S Transition....Pages 265-271 Transformation of NIH/3T3 and C3H/10T1/2 Cells by Citrinin and Ochratoxina....Pages 273-278 Senescence Induction in Cancer Cells....Pages 279-282 Growth Characteristics of NIH3T3 Cells Expressing bFGF and/or IGF-I and/or IGF-II....Pages 283-287 Targeted Disruption of a Mitochondrial Transcription Factor a in the Chicken DT40 Cell Lines....Pages 289-293 Expression and Characterization of Mouse Epidermal Transglutaminase in Baculovirus-Infected Insect Cells....Pages 295-299 Effect of Tea Polyphenols on Degranulation in Human Mature Basophils Differentiated with IL-4....Pages 301-305 Counteraction of the Active form of Vitamins A and D on Up-Regulation of Adipocyte Differentiation with PPARγ Ligand, a Thiazolidinedione, in 3T3-L1 Cells....Pages 307-311 CCK regulation of monitor peptide gene expression in pancreatic acinar AR42J cells....Pages 313-317 Detection of Mitochondrial Regulatory Region RNA in Cultured Cells and Differentiated Tissue Cells: The Implications for Cellular Growth Control....Pages 319-324 Effect of Ovarian Steroids and Oxytocin on Local Production of Prostaglandin E2, Prostaglandin F2α and Endothelin-1 in Cow Oviductal Epithelial Cell Monolayers....Pages 325-329 TGF-ß Induced Cellular Senescene in Cancer Cells is Reversible and Operates Via Two Separate and Independent Pathways....Pages 331-335 Molecular Cloning and Characterization of Specific Genes Involved in Cellular Senescence....Pages 337-341 Regulatory Mechanisms of Granulosa Cell Apoptosis in Ovarian Follicle Atresia....Pages 343-347 Enhancement of Fc&εRI Expression by Hydrocortisone Induces the Upregulation of Fc&εRI&γ mRNA in the Human Leukemia Cell Line KUS12....Pages 349-354 Telomere Shortening in Cancer Cells by Electrolyzed-Reduced Water....Pages 355-359 Acetic Acid Suppresses the Increase of Glycosidase Activity During Culture of Caco-2 Intestinal Epithelial Cells....Pages 361-365 Effects of Digested Skim Milk on the Proliferation Activity of Human Acute Myeloid Leukemia (HL-60) Cells....Pages 367-371 Differentiation-Inducing Activity of Human Leukemia Cell Lines by Extracts of Wild Plants, Seaweed and Mushrooms in Akita....Pages 373-377 Anti-Tumorigenic Protein from Aralia Elata....Pages 379-383 Suppression of W Damage-Induced Apoptosis of Human Melanoma Cells by a Fermented Milk, Kefir....Pages 385-389 Recognition System for Dietary Fatty Acids in the Rat Small Intestinal Cells and Taste Buds....Pages 391-395 Effect of Rose Bengal on Immunoglobulin Production by Mouse B Lymphoma, WEHI-279 Cells....Pages 397-401 Class — Specific Regulation of Immunoglobulin Production by Rose Bengal in Human B Cell Lines and Peripheral Blood Lymphocytes....Pages 403-407 Inhibitory Action of Carotenoids in Crab Shell on the Invasion of Hepatoma Cells Co-Cultured with Mesothelial Cells....Pages 409-413 The Neurite-Initiating Effect of Microbial Extracellular Glycolipids....Pages 415-419 Induction of Basophilic and Eosinophilic Differentiation in the Human Leukemic Cell Line KU812....Pages 421-425 Mannosylerythritol Lipid Induced Apoptosis and Differentiation of Melanoma B16 Cells....Pages 427-431 A Non-Radioactive In Vitro Bioassay for Recombinant Human IL-11....Pages 433-437 Establishment of Assay System for Immunoregulatory Factors Using Whole Cell Culture of Mouse Spleno-Cytes....Pages 439-443