Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Tenth Annual Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 5–8, 1997 Volume 10

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to ascertain their abilities to be used for industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins, and the application of animal cells to gene therapy and artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working either in academic environments or in industries of biotechnology and pharmacy. Booknews Held at Nyoga U. in November 1997, the meeting on the newly growing cell biology discipline of animal cell technology attracted 200 participants. The proceedings of the nine symposia, and numerous oral and poster sessions presenting the state-of-the-art in Japan should interest those working in the basic organic sciences and in biotechnology and pharmacy applications. Topics include: glycoengineering, artificial organ and cell therapy, transgenic livestock, gene transfer to cells and tissues of living animals, cell culture for assessment of physiologically functional substances, cost performance for producing biochemicals, applications of human monoclonal antibody, cell characterization and validation, transplantation and artificial organs, transgenic technology, and cytokines and signal transduction. The index spans AchE to zebrafish. Annotation c. Book News, Inc., Portland, OR (booknews.com) The Function of Dally, a Drosophila Glypican, in DPP Signaling....Pages 1-5 Novel Forms of Protein O-Glycosylation....Pages 7-14 Synthesis of Glycoconjugates: Their Use to Study the Intracellular Traffic of Endogenous Lectins and to Deliver Oligonucleotides and Genes....Pages 15-21 Oligo/Polysialic Acids in Sea Urchin: A Developmentally Regulated Sea Urchin Polysialyltransferase....Pages 23-26 Cloning and Application of Glycosyl Transferases Involved in the Biosynthesis of Selectin Ligands....Pages 27-31 Induction of Ganglioside Biosynthesis, Neurite Outgrowth and Functional Synapse Formation by a Symthetic Ceramide Analog....Pages 33-36 Human Cytotoxic T lymphocytes for Tumor Therapy....Pages 37-41 Organoid Formation with Branched Capillary-Like Networks....Pages 43-47 Cytotoxic Antibodies to Adipocytes: Agricultural and Clinical Potential....Pages 49-54 Immunological Manipulation of Lactation....Pages 55-61 Light Chain Shifting — Characterization of Light Chain Replacement in Human Plasma B Cells....Pages 63-67 Creation of Hybridomas from Mice Expressing Human Antibody by Introduction of a Human Chromosome....Pages 69-73 Transgenically Produced Biopharmaceuticals....Pages 75-79 Optimisation of Transgene Expression in the Mammary Gland....Pages 81-89 Introductory Remark: A Decade of Transgenic Fish....Pages 91-92 Transgenic Medaka Fish Strains with Lightened Body Color and Bioluminescence....Pages 93-96 Transgenic Zebrafish as a Biosensor....Pages 97-100 Transgenic Salmon for Aquaculture....Pages 101-105 Food Components with Potentialities to Suppress Proliferation and Invasion of Cancer Cells....Pages 107-111 Development of Thrombopoietin: Its Structure and Function....Pages 113-117 Baculovirus not only as an Insect Expression Vector but as a Gene Trasfer Vector....Pages 119-123 In Vivo Gene Electroporation: Manipulations of Cells to Whole Body of Living Animals....Pages 125-129 In Vitro Stimulation of Tumor Necrosis Factor Production from Rat Splenocytes by Cabbage Extract....Pages 131-134 Effects of Green, Oolong and Black Teas and Related Components on Proliferation and Invasion of Hepatoma Cells....Pages 135-139 Milk Secretion in Cultured Mammary Epithelial Cells....Pages 141-145 Hyperthermic Induction of Gene Expression Using Stress Inducible Promoter....Pages 147-152 Enhancement of Productivity of Recombinant α-Amidating Enzyme by Low Temperature Culture....Pages 153-157 Dialysis-fixed-bed Cultures for Highly Efficient Production of Chimeric Fab-fragments with Transfec Toma Cells in Protein-free Medium....Pages 159-163 Monitoring Drug Effects on Human Primary Tissues Utilizing Fibre Otical Sensors....Pages 165-169 Increasing Antibody Production by Suppressing Cell Proliferation with Controllable Expression of Cyclin Inhibitor P21 and P27....Pages 171-175 Establishment of an Apoptosis-Resistant and Growth-Controllable Cell Line by Transfecting With Inducible Antisense c-Jun Gene....Pages 177-184 Improvement of Cell Sirvoval by Inhibition of Caspases Playing Key Roles in Apoptosis....Pages 185-189 In Vitro Immunization of Human Peripheral Blood Lymphocytes with 14–16 kDa Rice Allergen Proteins....Pages 191-197 Options to Eliminate Animal-Derived Components of Cell Culture Media....Pages 199-203 Characterization of the Subpopulations of Rat Ascites Hepatoma AH109A Cells With Different Invasive and Metastatic Activities....Pages 205-209 Functional Expression of NMDA Receptor Channels in Differentiating Neural Precursor Cells....Pages 211-215 TGF-β Reversibly Induces Cell Senescence in Adenocarcinoma Cell Line....Pages 217-221 Evaluation of a Fixed-Bed Type Bioreactor System Using Recombinant CHO Cells Producing Human Transferrin....Pages 223-227 Formation of Cylindrical Multicellular Aggregate (Cylindroid) of Rat Hepatocytes on Pressed Sheet of Polyurethane Foam....Pages 229-233 Production of a Novel Recombinant TGFα-Like Fusion Protein for Use in Serum-Free Media....Pages 235-239 Effect of the Macromolecular Metabolism of Murine Hybridoma Cells on Antibody Productivity in Batch and Fed-Batch Culture....Pages 241-245 Effect of Glycine Betaine as Osmoprotectant on the Production of Erythropoietin by CHO Cells in Hyperosmotic Serum Free Media Culture....Pages 247-250 Establishment of Recombinant CHO Cell Lines Producing Wild-Type and Mutant Human α2-Macroglobulins ....Pages 251-257 Evaluation of Stability in the DHFR Gene Amplification System Using Fluorescence In Situ Hybridization....Pages 259-263 Expression and Characterization of Human Calpain and Calpastatin Using Baculovirus System....Pages 265-269 Evaluation of Spim Insect Cells Using a Novel Baculovirus Expression Vector System Employing the Hyphantria Cunea NPV....Pages 271-275 Development of a Hybrid Artificial Liver Support System with Porcine Hepatocyte Spheroids for Preclinical Use....Pages 277-281 In Vitro Self-Organization of Liver Cells Using Artificial Matrix....Pages 283-287 Thyroid Transplantation: Possibility of Application for the Treatment of Persistent Hypothyroidis and Study of Mechanisms of Interaction between Graft and Hypothalamic-Pituitary Axis of Recipient....Pages 289-293 Fixed-Bed Reactors as a Tool for Artificial Organs....Pages 295-297 Transcriptional Activities of Viral and Cellular Promoters In Vivo in the Oviduct of Living Chickens....Pages 299-302 Live Detection of the Firefly Luciferase Gene Expression in Bovine Preimplantation Embryos....Pages 303-306 Localized in vivo Gene Transfer in Sperma Togenic Cells of the Cryptorchid Mouse Testis....Pages 307-309 Preparation of a Bispecific Antibody to Ovomucoid and Horseradish Peroxidase....Pages 311-315 Vitamin a Acetate for Efficient Production of Monoclonal Antibody by Human-Human Hybridomas....Pages 317-321 Induction of Apoptosis by Osteopontin in MDCK Cells....Pages 323-327 Analysis of Glycosphingolipids on Differentiation-Induction of PC12 Pheochromocytoma Cells by Mannosylerythritol Lipid....Pages 329-333 Mannosylerythritol Lipid Induces Anti-Proliferation and Differentiation of Mouse Melanoma B16 Cells....Pages 335-339 Signal Transduction of Differentiation-Induction by Mannosylerythritol Lipid on PC 12 Pheochromocytoma Cells....Pages 341-345 Altered Expression of the CIP/KIP Family CDK Inhibitors in Tumor Phenotype-Suppressed Human Lung Cancer Cells....Pages 347-351 Screening for Bioactive Compounds that Induce the Differentiation of Bone Marrow-Derived Preadipocytic Cell Line, ST2, into the Cells of Osteoblastic Phenotype....Pages 353-356 Structure and Fuction of Succinoyl Trehalose Lipid on Differentiation-Induction of Human Promyelocytic Leukemia Cell Line HL-60....Pages 357-361 The Establishment of an Assay System for the Compounds Affecting Actin Ring Formation of Murine Osteoclast-Like Cells....Pages 363-367 Protection of Human Melandoma Cells from UV Damage by a Fermented Milk, Kefir....Pages 369-373 Identification of Specific Genes Involved in Cellular Senescence by Using Human Lung Adenocarcinoma Derived Cells....Pages 375-379 Laminin Chains Expressed in Human Keratinocytes, Mouse 3T3-LI Adipocytes and Mouse Embryonal Carcinoma F9....Pages 381-384