Animal Cell Technology: Basic & Applied Aspects : Proceedings of the Ninth Annual Meeting of the Japanese Association for Animal Cell Technology, Yokohama, Japan, September 1–4, 1996

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries. Front Matter....Pages i-xi Stress-Inducible Gene Expression in Fish Cells....Pages 1-4 Cell Culture and Some Properties of Fish Immunocytes....Pages 5-9 Telomerase regulation in differentiated serum-free mouse embryo cells....Pages 11-15 Immortalization of Mammalian Central Nervous System Stem Cells From p53 Knock-out Forebrain....Pages 17-22 Neuroprotective Function of Erythropoietin in the Central Nervous System....Pages 23-27 Selection and Stability for a Recombinant CHO Cell Line Expressing Human GM-CSF in Gene Amplification....Pages 29-33 Effect of Culture Conditions on Glycosylation in Hybridoma Antibody Production....Pages 35-39 Antisense Rb RNA Inhibits in Vitro Myogenic Differentiation in a Myoblast Cell Line, C2....Pages 41-44 Establishment of a Stable hGH Producing Verots S3 Transformant....Pages 45-49 Characterization of an Antigen-Producing Cell Line Hugk-14 at the Molecular Level....Pages 51-57 VJ Rearrangement at Previously Excluded Igλ Allele in Human Plasma Cell Line NAT-30....Pages 59-63 Rational Drug Design Using Mammalian Cell Lines Expressing Site-Directed Mutants of the Human H 1 Histaminic Receptor....Pages 65-69 Biologic Response of Endothelial Cells: Modulation of Adhesion and Multiplication by Cytokines and Prospects for the Production of a Vaccine Against the Rickettsia Cowdria ruminantium ....Pages 71-75 Effects of Chemicals on the Immune Response....Pages 77-81 Molecular Mechanisms of Selaginella Tamariscina for Antineoplastic Activity in Human Leukemia Cell Line U937....Pages 83-89 Differential Effect of Antigen Analogs with T cell Inhibitory Activity on in vivo Antibody Response....Pages 91-95 Increase of Intracellular Ca 2+ Level Induces Osteoclast- Like Cell Formation in Coculture of Mouse Bone Marrow Cells and Osteoblastic Cells....Pages 97-101 New Angiogenesis Inhibitors of Microbial Origin....Pages 103-107 Hydrocortisone Induces a Formation of Basophilic Granules in the Human Leukemia Cell Line KU812....Pages 109-113 Cytotoxicity Testing of Tomatine....Pages 115-119 Mannosylerythritol Lipid Induced Differentiation of Human Myelogenous Leukemia Cell Line K-562....Pages 121-125 Coffee’s Inhibitory Action on the Invasion of Hepatoma Cells Co-Cultured with Mesothelial Cells....Pages 127-130 Inhibitory Effects of Tea Catechins on Invasion of Human Fibrosarcoma HT1080 Cells to the Monolayer of Human Umbilical Vein Endothelial Cells....Pages 131-135 Norzoanthamine Suppresses PTH-Stimulated IL-6 Production in Vitro and Bone Resorption in Vivo....Pages 137-141 Preparation and Biological Activities of High Molecular Weight Substances from Dried Safflower Petals....Pages 143-148 Characterization of Human T Cell Clones Isolated from Immortalized T Cell Lines Generated by Oncogene Transfection....Pages 149-153 Acquired Autoantibodies from Human Plasma B Cells by Light Chain Shifting....Pages 155-159 Clinical Application of Anti-Carcinoma Human Monoclonal Antibody....Pages 161-164 Some Properties of Bispecific Antibodies to Bovine Lactoferrin and Horseradish Peroxidase Secreted by a Hybrid-Hybridoma HH1-4-3....Pages 165-169 Development of an Elisa Based Assay for Measuring Glycosyltransferase Activity Using Synthetic Glycopolymers....Pages 171-176 Concanamycin A, a Vacuolar Type H + -Atpase Inhibitor, Selectively Induces Cell Death in Activated CD8 + CTL....Pages 177-182 Yield Improvements Utilising Fed-Batch Cultivation and Optimisation Strategies....Pages 183-189 Quality Aspects of Batch Animal Cell Cultivation — Relevance to Fed-Batch Implementation in Industry....Pages 191-196 Technical Approaches to Minimize Regulatory Hurdles to Fed-Batch and Continuous Medium Supplementation....Pages 197-201 The Use Of Biosensors in Control of Fed-Batch Processes — Opportunities and Limitations....Pages 203-210 Metabolic System Engineering Approach for Saccharomyces cerevisiae and Hybridoma Cell Culture....Pages 211-216 Adaptive Fuzzy Control of Mammalian Cell Culture in Fed-Batch Reactor for Production of an Antithrombin III Variant....Pages 217-221 Cost-Saving Design and Operational Options for Large-Scale Production of Nutrient Medium and Buffers....Pages 223-227 Effect of Shear Stress on the rCHO Cells....Pages 229-233 Quail Embryo Culture Using Artificial Vessels....Pages 235-239 Enhancement of Transfection Efficiency Using Ligand-Modified Lipid Vesicles....Pages 241-245 Recombinant Protein Production by CHO Cells Cultured in a Chemically Defined Medium....Pages 247-252 Removal and Inactivation of Parvovirus from Protein Solution by a BMM Membrane Filter....Pages 253-259 Matricrine Effect of Basement Membrane Components on De Novo Formation of Mesenchymal Tissues....Pages 261-264 High-Cell-Density Suspension Perfusion Culture of Porcine Hepatocyte Spheroids for a Bioartificial Liver....Pages 265-269 Cold-Inducible Gene Expression in Fish Cells....Pages 271-274 Drosophila Cell Lines as the Source of Basement Membrane Components....Pages 275-278 The Binding Effect of Immune Complex by Rat Renal Glomerular Epithelial Cell Expressed with Human CR1 Gene....Pages 279-283 Preparation and Characterization of DNA/Anti DNA Antibody Immune Complex Using Monoclonal Antibodies....Pages 285-289 Morphology and Function of Rat Hepatocytes Cultured on Expanded Polytetrafluoroethylene Surface....Pages 291-293 Evaluation of Ectopic Bone Formation by Demineralized Allografts in 0.6HCL Using a Controlled-Heat Ultrasonic Cleaner....Pages 295-299 Reorganization of vascular wall cells in vitro ....Pages 301-305 Effects of Cell Morphology on Expression of Liver Specific Function and Hormone Response in Primary Hepatocyte Culture....Pages 307-311 HIV Vector Mediated Gene Transfer into CD4 Positive Cells....Pages 313-317 Enhancement of cellular productivity for a recombinant protein by a combination of promoter activation and gene amplification....Pages 319-323 A Tetracycline Regulated Recombinant Protein Production System....Pages 325-329 Improvement of Transient Protein Production By Cos-1 Cells Stably Overexpressing bcl-2 Gene....Pages 331-335 Effect of Cytokines on Growth of a Mouse Hybridoma Cell Line and its Antibody Production....Pages 337-342 Effect of Hypoosmotic Stress on Hybridoma Cell Growth and Antibody Production....Pages 343-347 Development of High Output Expression Vectors for Antibody Production in Mammalian Cells....Pages 349-354 Magnetoliposomes as Intracellular Heat Stress Mediators for the Temperature Sensitive Expression of Cytokine....Pages 355-359 Effect of Ammonium Ion on Glycoform of Erythropoietin Produced by Recombinant CHO Cells Using Lectin-Blotting Technique....Pages 361-365 Effect of Cloned Gene Dosage on the Expression Level and Stability of Chimeric Antibody Producing CHO Cells....Pages 367-371 A Novel Approach to Prepare Appropriate Antibodies for Immunoaffinity Chromatography....Pages 373-377 Characterization of Monoclonal Antibodies Produced by Hybridoma Cell Lines Prepared Against Hepatitis B Virus X Protein....Pages 379-384 Cultured-Animal-Cell Assay for Collagen-Synthesis-Activation Factor Produced by Callus of Woody Plant Eucommia Ulmoides ....Pages 385-389 Back Matter....Pages 391-398