Animal Cell Technology - Basic and Applied Aspects: Proceedings of the Fourth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 13-15 November 1991

New data on animal cell technology are brought together in this volume, with emphasis given to the basic characterization of cell lines. The merits of different cell culture systems are examined and investigations into the factors influencing cell growth and productivity are presented. A special section deals with the biological properties of proteins produced by engineered animal cells. All those involved in the culture of animal cells will find this volume invaluable. Front Matter....Pages i-xiii The Value of Models in Animal Cell Biotechnology....Pages 1-5 The Manzanar Project — A Unique Way to End World Hunger....Pages 7-8 Experience in Cell Line Testing....Pages 9-14 Virus Removal and Inactivation in Process Validation....Pages 15-30 Multilocus DNA Fingerprinting in the Analysis of Cell Stocks: Stability Studies and Application to a Wide Range of Species....Pages 31-39 Alteration of Cell Surface Glycoproteins through Differentiation from a Human Histiocytic Lymphoma Cell Line, U-937, to a Macrophage-Like Cell Line, U-M....Pages 41-47 Removability of Virus Particles and Permeability of Protein from Cell Culture Medium Using Cuprammonium Regenerated Cellulose Hollow Fiber (BMMTM)....Pages 49-55 An Evaluation of Hybridoma Cell-Specific Productivity: Perfused Immobilized, Continuous Suspension, and Batch Suspension Cultures....Pages 57-63 Dynamics of Aggregate Culture of Mammalian Cells....Pages 65-69 Effect of Protein Adsorption on Cell Attachment on Solid Surfaces....Pages 71-76 Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriers....Pages 77-80 Application of Three Dimensional Culture of Adult Rat Hepatocytes in PUF Pores for Artificial Liver Support System....Pages 81-86 Novel Validation Method of Virus-Removability for Biological Cell Culture Products Using Polymeric Membrane Filters....Pages 87-102 Selective Formation and Immobilization by Re-Attachment of Floating Spheroids of Rat Hepatocytes....Pages 103-108 Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Production....Pages 109-114 Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture System....Pages 115-120 Oscillatory Behavior in Continuous Aggregate Cultures of Mammalian Cells....Pages 121-127 Detection of Mammalian Cell Attachment and Lysis on a Metal Surface Using a Piezoelectric Sensor....Pages 129-134 Strategies for Economic GMP Production of Mammalian Cell Expressed Proteins....Pages 135-141 Use of Medium Concentrates to Improve Bioreactor Productivity....Pages 143-148 Separation of animal cells in continuous cell culture systems....Pages 149-154 An Automated System for Hollow Fiber Cell Culture....Pages 155-164 Automatic Selection System of Monoclonal Antibody Producing Cells....Pages 165-171 Continuous Monoclonal Antibody Production by a Composite Gel Perfusion Bioreactor in a Protein-Free Medium....Pages 173-178 High Density Cultivation of Hybridoma Cells by Using Permeable Capillaries....Pages 179-184 Recognition and Counting Method of Mammalian Cells on Micro-Carrier Using Image Processing and Neural Network....Pages 185-191 Mitochondrial Genome Transcription in Cultured Animal Cells....Pages 193-200 Large-Scale Mammalian Cell Cultures by Application of Macroporous Cellulose Carriers....Pages 201-207 High Density Perfusion Culture of Animal Cells Using a Novel Continuous Flow Centrifuge....Pages 209-216 Membrane Dialysis Bioreactor with Integrated Radial-Flow Fixed Bed — A New Concept for Continuous Cultivation of Animal Cells....Pages 217-223 Effect of Glutamine Supplementation on the Growth of Hybridoma Cells Cultivated in a Reactor Coupled with an Ammonia Removing System....Pages 225-231 Virus Containment Continuous Culture of Animal Cells....Pages 233-237 A Novel Bioreactor Process for Anchorage-Independent Animal Cells Immobilized Within Porous Biomass Support Particles....Pages 239-244 Effects of Environmental Parameters on CHO Cell Growth in Batch and Chemostat Cultures and on Human IFN-γ Synthesis and Fidelity....Pages 245-249 Design and Characterization of Electrically Controlled Bioreactor.....Pages 251-256 Modulation of the Proliferation of Cultured Hepatoma Cells by Urea Cycle-Related Amino Acids....Pages 257-263 The Effects of Glutamine Concentration on Growth and Monoclonal Antibody Production in Fed-Batch Operation....Pages 265-270 Stimulation of Animal Cell Metabolism by Shear Stress....Pages 271-277 Effects of Conditioned Medium on the Growth Kinetics and the Monoclonal Antibody Productivity of Hybridoma Cells....Pages 279-285 Factors influencing expression and post-translational modification of recombinant protein C....Pages 287-293 A Simple Automatic Control System of pH and DO....Pages 295-302 Hybridoma Culture in Iron-Rich Protein-Free Medium: Cell Growth, Apoptosis, and Nature of Macromolecules Recovered from the Culture Fluid....Pages 303-309 A Novel Vero Cell Line for Use as a Mammalian Host-Vector System in Serum-Free Culture....Pages 311-319 Serum Free Culture of Human-Human Hybridoma Secreting Monoclonal Antibodies Against Stomach Cancer....Pages 321-326 Serum-Free Media for the Growth and Maintenance of the Differentiated State of Keratinocytes, Hepatocytes, and Neural Cells....Pages 327-334 Characterization of an Airway Epithelial Cell from Neonatal Rat....Pages 335-343 Production of Human Antithrombin-III in a Serum-Free Culture of CHO Cells....Pages 345-351 A Novel Protein- and Hormone-Free Medium: Its Application to Hybridoma Culture and Monoclonal Antibody Production....Pages 353-359 EDF (Erythroid Differentiation Factor = Activin A) Production in Recombinant CHO Cells....Pages 361-367 A Novel Serum Free Medium for Mouse Hybridomas and Human Lymphoblastoid Cell Lines....Pages 369-374 A Simple Structured Model Predicted Positively-, Negatively-, or Non-Growth Associated Antibody Production Rate Depending on Culture Conditions....Pages 375-381 Change in Monoclonal Antibody Productivity in Immobilized Hybridoma Cells at Low Dissolved Oxygen Concentrations....Pages 383-389 Improved Monoclonal Antibody Production of a Hybridoma by Varying the Oxygen Tension during a Suspension Culture....Pages 391-396 Enhancement of Productivity by Yield Improvements Using Simulation Techniques....Pages 397-403 Studies on the Production of Recombinant Human NF-IL6 in Mouse L Cell....Pages 405-412 Enhancing Effect of Interleukin-6 on Antibody Productivity of a Hybridoma Cell Line....Pages 413-418 Improved Production of Recombinant Proteins by the Baculovirus Expression System....Pages 419-424 Production and Product Integrity of Recombinant HIV-1 gag -Particles (p55) with the Baculovirus Expression System....Pages 425-430 Enhancement of Biological Productivity in Serum-Free Culture....Pages 431-438 Enhancement of IgM Production of HB4C5 Cells by Chitosan....Pages 439-443 Repression of Ovalbumin Gene Expression in the Chicken Oviduct Cell....Pages 445-451 Constitutive Long-Term Production of Recombinant Human Interferon-Gamma by Transformed Mouse C127 Cells Cultured in Serum Free Medium....Pages 453-462 Possible Involvement of Protein Tyrosine Sulfation in the Polarized Protein Targeting in Filter-Grown Madin-Darby Canine Kidney (MDCK) Cells....Pages 463-471 Protein Tyrosine Sulfation in Animal Cells and its Simulation in Vitro in the Downstream Processing of Proteins....Pages 473-479 Expression of Carp α-Globin Gene in Tilapia Ovary Cells....Pages 481-489 Immunosuppressive Functions of Cells from Intestinal Tissues....Pages 491-497 Biological Functions of Carbohydrates in Erythropoietin....Pages 499-507 Cloning and Expression of Flatfish Interleukin 2 Gene....Pages 509-514 Serum-Free Mouse Embryo (SFME) Cells and Transforming Growth Factor (TGF)β1....Pages 515-519 Anti-Tetanus Human Monoclonal Antibodies-Comparative Studies on their Protective Activity with Polyclonal Antibody....Pages 521-525 Production of chicken monoclonal antibody....Pages 527-534 Generation and Characterization of Murine Anti-Human Transforming Growth Factor-β1 Monoclonal Antibodies....Pages 535-539 Examination of lung cancer using a human monoclonal antibody HB4C5-clone 3....Pages 541-545 Alteration of Reactivity of Human Monoclonal Antibodies Produced by Concanavalin A-Resistant Hybridomas....Pages 547-551 Development of Mouse Fibroblast Dermal Equivalent Using Tissue Culture Technique....Pages 553-558 Effects of Some Food Additives and Mycotoxins on the Growth of HuH-6KK Cells....Pages 559-563 Europium-Linked Immuno Assay: Production of Immunogloblin by Human-Human Hybridoma Cell Lines....Pages 565-570 The Protective Role of Polyphenols in Cytotoxicity of Hydrogen Peroxide....Pages 571-576 Back Matter....Pages 577-584 Proceedings of the Fourth Annual Meeting of the Japanese Association for Animal Cell Technology, Fukuoka, Japan, 13-15 November 1991