Animal cell technology : basic and applied aspects : proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka, Japan, November 24-27, 2008

Animal cell technology is a growing discipline of cell biology which aims not only to understand the structure, function and behavior of differentiated animal cells, but also to ascertain their ability to be used for industrial and medical purposes. Some of the major goals of animal cell technology include: the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability for the production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of the present state-of-the-art research in Japan and other countries where this field is well advanced. The Proceedings will be useful to cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and to those working in either academic environments or in the biotechnology and pharmacy industries related to animal cell culture. Front Matter....Pages i-xxx Purification of Hepatoblasts from Fetal Mouse Livers by Using a Temperature-Reversible Gelation Polymer and Their Application in Regenerative Medicine....Pages 1-6 Computer Aided Rational Design of Biochemical Systems....Pages 7-12 DNA Microarray in Nutrigenomics and Cancer Prevention Research: Elucidating Molecular Signatures of Cancer Preventive Phytochemicals....Pages 13-18 DNA Microarray Technology Evaluation of Effect of Dietary Intake of Crude Rice Glycosphingolipids in Mice....Pages 19-25 Integrated Pathway Analysis of Genome-Wide Expression Changes Associated with Serum-Free Suspension Adaptation of an Antibody-Producing Chinese Hamster Ovary (CHO) Cell Line....Pages 27-32 Affordable Mab by Ensuring Low Cog....Pages 33-39 Evaluation of Neurotoxicity of Environmental Chemicals Using Neural Stem Cells and Neuroblastoma Cells....Pages 41-44 Integration of Endothelial Cell-Covered Hepatocyte Spheroids for Construction of Vascularized Liver Tissue....Pages 45-50 Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines....Pages 51-55 Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage-Dependent Cells....Pages 57-63 Advantage of Alagln as an Additive to Cell Culture Medium....Pages 65-71 Supplementation of Phosphatidylcholine Protects the Hybridoma Cells from Apoptosis Induced by Serum Withdrawal....Pages 73-76 Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition....Pages 77-81 Efficient Selection of Cell Clones with Higher Productivity in the Production of Recombinant Human Monoclonal Antibodies....Pages 83-88 Continuous Cell Production from Three Dimensional Hematopoietic Microenvironment in Polyurethane Foam....Pages 89-94 A New Approach for Drug Discovery and Differentiation Study Using Cutting-Edge 3d Cell Culture System....Pages 95-102 Dimensional Cell Culture Dish for In Vivo-Like Cell Culture....Pages 103-108 Characterization of the Erythropoietin (EPO) Protein Production in the Recombinant Human Cultured Cells, in which the Exogenous EPO Gene was Introduced into Hypoxanthine Phosphoribosyl Transferase (HPRT) Gene Locus....Pages 109-114 Development of Protein High Expression System by Using Fructose Metabolism....Pages 115-119 Controlling Fucosylation Levels of Antibodies with Osmolality during Cell Culture....Pages 121-125 Comparison of Immunoresponses Between Cecal Patch Cells and Peyer’s Patch Cells Stimulated by Bacterial Components....Pages 127-131 Conjugate of β -Lactoglobulin with Carboxymethyl Dextran Increased the Antigen-Specific IgG1 Production, While It Decreased IgE Production Compared with Native β -Lactoglobulin ....Pages 133-137 Anti-Inflammatory Action of Epigallocatechin-3-Gallate at a Physiological Concentration through the 67-KDA Laminin Receptor in Lipopolysaccharide-Stimulated Raw264.7 Cells....Pages 139-144 Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production....Pages 145-150 Production of Human Monoclonal Antibodies Against FC Epsilon Receptor I Alpha by Combining In Vitro Immunization with Phage Display....Pages 151-157 Enhancement of Hepatocyte Function Through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells....Pages 159-163 Magnetic Cell-Patterning for Tissue Engineering....Pages 165-170 Magnetic Force-Based Tissue Engineering of Skeletal Muscle for Bio-Actuator....Pages 171-176 Fabrication of Skeletal Muscle Tissue from C2C12 Myoblast Cell Towards the Use as Bio-Actuator....Pages 177-183 Retroviral Vectors Pseudotyped with Chimeric Vesicular Stomatitis Virus Glycoprotein for Antibody-Dependent Gene Transduction....Pages 185-190 Improvement of CPP-RBD for siRNA Delivery....Pages 191-196 Production of Recombinant Human EPO and EPO/Fc Fusion Proteins by Chinese Hamster Ovary Cells....Pages 197-202 Development of Oviduct-Specific Gene Expression System for Transgenic Avian Bioreactor....Pages 203-208 Production of Therapeutic Proteins Composed of Seven Dominant Human T Cell Epitopes Derived from the Japanese Cedar Pollen Allergens....Pages 209-214 The Differentiation of C2C12 Cells to Myotube by Paraquat....Pages 215-219 Molecular Cloning and Characterization of a Novel Canine Sulfotransferase....Pages 221-229 In Vitro Analysis of the Interaction Between Human Intestinal Epithelial Cells and Macrophage-Like Cells....Pages 231-236 Role of Ubiquitination Promoted During Restimulation in the Induction of T Cell Anergy....Pages 237-241 Expression Analysis of Senescence-Associated Genes: Their Possible Involvement in Diabetes....Pages 243-246 Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem-Like Cells....Pages 247-252 Growth Control of Mammalian Cells via a Human Artificial Chromosome Harboring a Chimeric Receptor....Pages 253-258 The Impact of Redox State on Regulation of the High-Affinity IgE Receptor Expression....Pages 259-264 The Characteristic of Chimeric Receptors Based on Erythropoietin Receptor....Pages 265-270 Anti-Cancer and Structure-Activity Relationship of Natural Polyacetylenes....Pages 271-275 Effect of Mongolian Medicinal Plant Stellera Chamaejasme on Chronic Leukemia Cells K562....Pages 277-280 Capsaicin Induced the Upregulation of Transcriptional and Translational Expression of Glycolytic Enzymes Related to Energy Metabolism in Human Intestinal Epithelial Cells....Pages 281-283 Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis....Pages 285-287 Electrolyzed Reduced Water Prolongs Caenorhabditis elegans’ Lifespan....Pages 289-293 Anti-Cancer Effects of Enzyme-Digested Fucoidan Extract from Seaweed Mozuku....Pages 295-300 The Downregulation of Mast Cell Activation Through the Suppression of the High-Affinity IgE Receptor Expression by Green Tea Catechin Egcg....Pages 301-306 Anti-Diabetes Effects of Hita Tenryou-Sui Water ® , a Natural Reduced Water....Pages 307-313 Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level....Pages 315-321 Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen ....Pages 323-325 Insulin-Mimetic Activity of Inositol Derivatives Depends on Phosphorylation of PKCζ/λ in L6 Myotubes....Pages 327-331 Enhancement of Phagocytic Activity by a Crude Polysaccharide from Tea ( Camellia sinensis ) Extract....Pages 333-338 Effects of Lupeol on Melanoma In Vitro and In Vivo: Fundamental and Clinical Trials....Pages 339-344 The Effect of Secoisolariciresinol on 3t3-L1 Adipocytes and the Relationship Between Molecular Structure and the Activity....Pages 345-351 Detection of Anti-Allergic Factors in Strawberry Extracts....Pages 353-357 Anti-Melanogenic Activity of Yacon Leaves in Mouse Melanoma Cells....Pages 359-364 Cytokine Responses of Splenocytes of Female and Male Non-Obese Diabetic Mice Induced by Lactic Acid Bacteria....Pages 365-370 Development of a High-Throughput Evaluation System for Antiviral Activities of Food Factors By Using Bioinformatics....Pages 371-376 The Effects of Pesticides on Immune Cells....Pages 377-382 Co-Cultured Hepatocyte-Spheroid Chip Constructed Using the Microfabrication Technique....Pages 383-389 Micropatterned Hepatocyte Culture Using Microstencil Technique....Pages 391-396 Structural Features of Totally Designed Lipidic Materials for Small Interfering RNA Delivery System....Pages 397-402 Thin-Film Assembly of Totally Designed Lipidic Materials as Gene Carrier in Mouse Embryonic Neural Stem Cells: Thin-Film Assembly of Lipidic Material as Potential Gene Carrier....Pages 403-408 Transfection System Using a Cocktail of Totally Designed Lipidic Materials in Chinese Hamster Ovary Cells: Lipidic Cocktail as Potential Gene Carrier for CHO Cells....Pages 409-414 Size Dependent Heat Generation of Magnetic Nanoparticles Under AC Magnetic Field for Cancer Therapy....Pages 415-421 Functional Evaluation of Anticancer Drugs in Human Leukemia Cells Based on Metabolic Profiling Technique....Pages 423-428 Extracts from the Root of Platycodon grandiflorum Modulate Induction of Inflammatory Changes in Coculture of Adipocytes and Macrophages....Pages 429-434 Position Specific Incorporation of Nonnatural Amino Acids into Proteins In Vivo....Pages 435-439 Novel Protease Inhibitor Inhibiting Proliferation of Influenza Virus: Purification and cDNA Cloning of a Novel Protease Inhibitor Secreted by MDCK Cells....Pages 441-445