روش‌های کشت سلول‌های حیوانی (روش‌ها در زیست‌شناسی سلولی، جلد 57)

This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Reviews basic principles of cell culture * Gives options and techniques on how to look at cells Content: Series Editors Page ii Edited by Page iii Copyright Page Page iv Contributors Pages xi-xii Preface Pages xiii-xiv Jennie P. Mather, David Barnes Principles of Cell Culture Pages 1-2 Chapter 1 Animal Cell Culture Equipment and Techniques Original Research Article Pages 3-17 Angela Helmrich, David Barnes Chapter 2 Making Informed Choices: Medium, Serum, and Serum-Free Medium How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create Original Research Article Pages 19-30 Jennie P. Mather Chapter 3 Cell Line Availability: Where to Get the Cell Lines You Need Original Research Article Pages 31-47 Robert J. Hay Chapter 4 Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination Original Research Article Pages 49-65 Carolyn Kay Lincoln, Michael G. Gabridge Establishing Cell Lines Pages 67-68 Chapter 5 Immortalization by Gene Transfection Original Research Article Pages 69-91 Yoshinori Katakura, Shahabuddin Alam, Sanetaka Shirahata Chapter 6 Establishment of Mammalian Testicular Cell Lines Original Research Article Pages 93-110 Marie-Claude C. Hofmann, Jose Luis Millan Chapter 7 Cell Hybridization, Hybridomas, and Human Hybridomas Original Research Article Pages 111-145 Sanetaka Shirahata, Yoshinori Katakura, Kiichiro Teruya Chapter 8 Establishing Human Glioma-Derived Cell Lines Original Research Article Pages 147-165 Manfred Westphal, Hildegard Meissner Chapter 9 Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells Original Research Article Pages 167-186 Ronghao Li Chapter 10 Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms Original Research Article Pages 187-201 Christopher J. Bayne Chapter 11 Cell Line Characterization and Authentication Original Research Article Pages 203-216 Joseph Kaplan, Bharati Hukku Specialized Culture Techniques Pages 217-218 Chapter 12 Laboratory Scaleup of Cell Cultures (0.5-50 Liters) Original Research Article Pages 219-227 Jennie P. Mather Chapter 13 Cell Synchronization Original Research Article Pages 229-249 Gary F. Merrill Chapter 14 Measurement of Cell Death Original Research Article Pages 251-264 Deryk T. Loo, Jill R. Rillema Chapter 15 Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death Original Research Article Pages 265-278 Alison Moore, Christopher J. Donahue, Kenneth D. Bauer, Jennie P. Mather Chapter 16 Embryonic Stem Cells, Creating Transgenic Animals Original Research Article Pages 279-293 Melinda Pirity, Anna-Katerina Hadjantonakis, András Nagy Microscopy and Morphology Page 295 Chapter 17 Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture Original Research Article Pages 297-311 David M. Phillips Chapter 18 Indirect Immunofluorescence Microscopy in Cultured Cells Original Research Article Pages 313-332 Sally P. Wheatley, Yu-li Wang Chapter 19 Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and in Situ Ligand Binding Original Research Article Pages 333-351 Teresa K. Woodruff Index Pages 353-361 Volumes in Series Pages 363-368 This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features* Reviews basic principles of cell culture* Gives options and techniques on how to look at cells Provides complete coverage of the classical and state-ofthe art techniques used in studies of cultured cells. Includes the basic principles of cell culture as well as discussions of the criteria used to make appropriate choices for establishing a model system. Hardcover, plastic-comb bound version available. This chapter is devoted to some of the basics of cell culture equipment and techniques.